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The map label for this gene is pilS [H]

Identifier: 73542628

GI number: 73542628

Start: 3229445

End: 3231562

Strand: Reverse

Name: pilS [H]

Synonym: Reut_A2944

Alternate gene names: 73542628

Gene position: 3231562-3229445 (Counterclockwise)

Preceding gene: 73542629

Following gene: 73542627

Centisome position: 84.9

GC content: 68.93

Gene sequence:

>2118_bases
ATGAGCACGGCGCGGCCGACGATCACCGCCCGGCACGCCCCGTGGCTCGGCTTTGCCCAACTGTGGCGAGCGCCCGAGCC
GCCTGATTTCCACTGGCGCCTGCTGCGCTATTTCTGCTGGACCCGCGTGGCCGTGGGCCTGCTGTTGCTGGCCTTCACCA
TCATGCAGCGAGGCGCGGATACGCACGGCGTGTCGCTCGTGCTGCCGTTCCAGCTCACCTTGCCCGCCCACACGACGGCG
GCCATGCAACTGGCCATCCCTTACCTTGGCATCGCGCTCGCCATGCTGGCGGGCACGTTGTGGCGCAGGCGTTTCCATTT
GCGCGTGCGGCTGCAGGTGCTGGCCGATCTGGCCATGCTTGGCCTGATCTACGAGATGCTCGGCCGCAACGGCCATGCCG
ATGGCCTGGCGATGATCTTCCTGCTGCCGGCGCTGGAAGCCGGCGCGCTCACCAGCCTGCTGTTCGCGCTGTTTGCCGCC
TCGCTCTCGGCGCTGGTGATCATGGCGCACCCGTTCATGCAGACCATCCTGCTGCGCCAGGCCGATGCCGGCCTGCTCGG
CTCGGGCCTGTACGGGCTGGTGTTCATGATCGCGGCGCTGCTGATGTATATGCTGGCCTACCGGCAGGTCGCGCAGGAAA
AGATCGCGCTCGCGCGCGAGCAGGAGCTGCGCCTGCAGCAGCTCGTGAACCGGCTGATGGTCAACGACATGCAGGATGGC
GTGATGCTGGTGCGCGCCAATGGCATGGTCGTGGCCGCCAACCCGGCCGCCGTGGTGCTGCTGGGCGTGCAGCCGCATGA
GCGCGTCTATTCGCGCCGCGGCCGCGAAGGCGAGGGCGACGAAGGTGGCCAGGCCGCGCTGTTCGACCTGCGCCGCATTC
CGCGCCTGCAGCCGCTCATGGAAATGCTGCGGGACTGGCTGCGCAGCAAGGACGATGTGCCCCGCATCCTGCAACTGCTG
CCCATCGTCTCGCGTCCGGCGCCGCCCGCCGGCGGCACGCTGCACACGCGGCTGCGCCTGCGTTTCGTGCTGCCGGGGCT
GGCCGGGTTGCGTGCCACCGTGGCAAGCACCTCGACGATGCCATCGAACCTCGACACGGCCGCCTGGAACGAACTGACGC
CCGAAGCCGCCGCGCGCCTGCGCCTGGCCATTGCCCAGCAGGAAGCCATGGCCTGGACGCCCGACGACGAGGCGCTGCTG
CGCAGCGAGATGCGCGACACCGTGCTGGTCCACATCGAAAGCTGGGAACGCATCGCCGAACAGGTCCAGCAGGAAAAGCT
CGCCGCCATGGGCCGGCTGGTGGCCAGCGTTGCGCACCAGATCCGCAACCCGCTGGCCGCGATCAGCCAGGCCAGCGAAC
TACTCGGCGACGGCGCCAGCATGGGCAGCGGCGGCGGCGTGGAGTCCGATGTCGATGCGCGCCTGCTGCGCATCATCCAT
GACAATGTGCGCCGGCTCGACCAGGTCGTGTCAGACGTGCTGCAGATCTCGCGCCGCCCGCGCACGGGCCGCAGCACCGT
GCACCTGGCGCAGGCGCTGCCCGAGATCGTCGACCGCTGGCGCCTGGAAATGCGCTTGCGCGTGCCGCGCGGCGGTGACA
CGCGCCAGCCCGGACGCGGCGAGATCAATGCGAATGCCATCCGGCTTACGGTAGACGTGCCGCAGCAGGTTGTCTTCGAT
ACCTCGCAGCTCCAGCAGGTGCTTGGGAACCTGCTCGACAACGCATGGCGCTATTGCAGCCGCCTGCCGGGCGCCATCCG
CCTGCTCGCGCATGCGCTGGACCAGCACCATGCCGAGCTGATCGTCTGGAACGACGGCACGGAGATTCCGCGCGAGCACC
AGCGCAGCCTGTTCGAGCCGTTCTTTACCAGCAATGCACAGGGCACCGGGCTCGGCCTGTTCATGGCGCGCGAGCTATGC
GGCGCTAATGACGCGCAGGTCCGCTACGGCACCATCGCACTCGACGACCTGCTCGCGCGCACCGGACTGCTGGCTGCGCC
GCGCGATACACTGCCTGCCAAGGCCTTCGTGCTGACGCTGCGCATCGACACCGGCGATGCGGCGCCGGCCCGAGGCGCCG
ATCCGCTTGTCCACCGAAACTCCGCCTCCGATACGTAG

Upstream 100 bases:

>100_bases
CCCATTGCGGCGTGCACCTGCCGAAGAGCGAGGCCATTGCCTGGCGCGGCCTGCATTACTGCCGGCGCAGCCACTTGCCC
GACGACAGCGGCGACCGCGC

Downstream 100 bases:

>100_bases
CTCCATGCCACCCAGAACGCCTGGCCTCGATCCCATCCTCGTCATCGACGACGAGGCAGACTTGCGCGAGCTGCTGGACA
TTTCCATCCGCCGCATGGGG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 705; Mature: 704

Protein sequence:

>705_residues
MSTARPTITARHAPWLGFAQLWRAPEPPDFHWRLLRYFCWTRVAVGLLLLAFTIMQRGADTHGVSLVLPFQLTLPAHTTA
AMQLAIPYLGIALAMLAGTLWRRRFHLRVRLQVLADLAMLGLIYEMLGRNGHADGLAMIFLLPALEAGALTSLLFALFAA
SLSALVIMAHPFMQTILLRQADAGLLGSGLYGLVFMIAALLMYMLAYRQVAQEKIALAREQELRLQQLVNRLMVNDMQDG
VMLVRANGMVVAANPAAVVLLGVQPHERVYSRRGREGEGDEGGQAALFDLRRIPRLQPLMEMLRDWLRSKDDVPRILQLL
PIVSRPAPPAGGTLHTRLRLRFVLPGLAGLRATVASTSTMPSNLDTAAWNELTPEAAARLRLAIAQQEAMAWTPDDEALL
RSEMRDTVLVHIESWERIAEQVQQEKLAAMGRLVASVAHQIRNPLAAISQASELLGDGASMGSGGGVESDVDARLLRIIH
DNVRRLDQVVSDVLQISRRPRTGRSTVHLAQALPEIVDRWRLEMRLRVPRGGDTRQPGRGEINANAIRLTVDVPQQVVFD
TSQLQQVLGNLLDNAWRYCSRLPGAIRLLAHALDQHHAELIVWNDGTEIPREHQRSLFEPFFTSNAQGTGLGLFMARELC
GANDAQVRYGTIALDDLLARTGLLAAPRDTLPAKAFVLTLRIDTGDAAPARGADPLVHRNSASDT

Sequences:

>Translated_705_residues
MSTARPTITARHAPWLGFAQLWRAPEPPDFHWRLLRYFCWTRVAVGLLLLAFTIMQRGADTHGVSLVLPFQLTLPAHTTA
AMQLAIPYLGIALAMLAGTLWRRRFHLRVRLQVLADLAMLGLIYEMLGRNGHADGLAMIFLLPALEAGALTSLLFALFAA
SLSALVIMAHPFMQTILLRQADAGLLGSGLYGLVFMIAALLMYMLAYRQVAQEKIALAREQELRLQQLVNRLMVNDMQDG
VMLVRANGMVVAANPAAVVLLGVQPHERVYSRRGREGEGDEGGQAALFDLRRIPRLQPLMEMLRDWLRSKDDVPRILQLL
PIVSRPAPPAGGTLHTRLRLRFVLPGLAGLRATVASTSTMPSNLDTAAWNELTPEAAARLRLAIAQQEAMAWTPDDEALL
RSEMRDTVLVHIESWERIAEQVQQEKLAAMGRLVASVAHQIRNPLAAISQASELLGDGASMGSGGGVESDVDARLLRIIH
DNVRRLDQVVSDVLQISRRPRTGRSTVHLAQALPEIVDRWRLEMRLRVPRGGDTRQPGRGEINANAIRLTVDVPQQVVFD
TSQLQQVLGNLLDNAWRYCSRLPGAIRLLAHALDQHHAELIVWNDGTEIPREHQRSLFEPFFTSNAQGTGLGLFMARELC
GANDAQVRYGTIALDDLLARTGLLAAPRDTLPAKAFVLTLRIDTGDAAPARGADPLVHRNSASDT
>Mature_704_residues
STARPTITARHAPWLGFAQLWRAPEPPDFHWRLLRYFCWTRVAVGLLLLAFTIMQRGADTHGVSLVLPFQLTLPAHTTAA
MQLAIPYLGIALAMLAGTLWRRRFHLRVRLQVLADLAMLGLIYEMLGRNGHADGLAMIFLLPALEAGALTSLLFALFAAS
LSALVIMAHPFMQTILLRQADAGLLGSGLYGLVFMIAALLMYMLAYRQVAQEKIALAREQELRLQQLVNRLMVNDMQDGV
MLVRANGMVVAANPAAVVLLGVQPHERVYSRRGREGEGDEGGQAALFDLRRIPRLQPLMEMLRDWLRSKDDVPRILQLLP
IVSRPAPPAGGTLHTRLRLRFVLPGLAGLRATVASTSTMPSNLDTAAWNELTPEAAARLRLAIAQQEAMAWTPDDEALLR
SEMRDTVLVHIESWERIAEQVQQEKLAAMGRLVASVAHQIRNPLAAISQASELLGDGASMGSGGGVESDVDARLLRIIHD
NVRRLDQVVSDVLQISRRPRTGRSTVHLAQALPEIVDRWRLEMRLRVPRGGDTRQPGRGEINANAIRLTVDVPQQVVFDT
SQLQQVLGNLLDNAWRYCSRLPGAIRLLAHALDQHHAELIVWNDGTEIPREHQRSLFEPFFTSNAQGTGLGLFMARELCG
ANDAQVRYGTIALDDLLARTGLLAAPRDTLPAKAFVLTLRIDTGDAAPARGADPLVHRNSASDT

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=243, Percent_Identity=26.7489711934156, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1790436, Length=222, Percent_Identity=28.3783783783784, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 77736; Mature: 77604

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTARPTITARHAPWLGFAQLWRAPEPPDFHWRLLRYFCWTRVAVGLLLLAFTIMQRGAD
CCCCCCCEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
THGVSLVLPFQLTLPAHTTAAMQLAIPYLGIALAMLAGTLWRRRFHLRVRLQVLADLAML
CCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLIYEMLGRNGHADGLAMIFLLPALEAGALTSLLFALFAASLSALVIMAHPFMQTILLRQ
HHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADAGLLGSGLYGLVFMIAALLMYMLAYRQVAQEKIALAREQELRLQQLVNRLMVNDMQDG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VMLVRANGMVVAANPAAVVLLGVQPHERVYSRRGREGEGDEGGQAALFDLRRIPRLQPLM
EEEEEECCEEEEECCCEEEEECCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHH
EMLRDWLRSKDDVPRILQLLPIVSRPAPPAGGTLHTRLRLRFVLPGLAGLRATVASTSTM
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHEEHHHCCCHHHHHHHHHCCCCC
PSNLDTAAWNELTPEAAARLRLAIAQQEAMAWTPDDEALLRSEMRDTVLVHIESWERIAE
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEHHHHHHHHH
QVQQEKLAAMGRLVASVAHQIRNPLAAISQASELLGDGASMGSGGGVESDVDARLLRIIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
DNVRRLDQVVSDVLQISRRPRTGRSTVHLAQALPEIVDRWRLEMRLRVPRGGDTRQPGRG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCC
EINANAIRLTVDVPQQVVFDTSQLQQVLGNLLDNAWRYCSRLPGAIRLLAHALDQHHAEL
CCCCCEEEEEEECCHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEE
IVWNDGTEIPREHQRSLFEPFFTSNAQGTGLGLFMARELCGANDAQVRYGTIALDDLLAR
EEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHH
TGLLAAPRDTLPAKAFVLTLRIDTGDAAPARGADPLVHRNSASDT
HCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEECCCCCCC
>Mature Secondary Structure 
STARPTITARHAPWLGFAQLWRAPEPPDFHWRLLRYFCWTRVAVGLLLLAFTIMQRGAD
CCCCCCEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
THGVSLVLPFQLTLPAHTTAAMQLAIPYLGIALAMLAGTLWRRRFHLRVRLQVLADLAML
CCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLIYEMLGRNGHADGLAMIFLLPALEAGALTSLLFALFAASLSALVIMAHPFMQTILLRQ
HHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADAGLLGSGLYGLVFMIAALLMYMLAYRQVAQEKIALAREQELRLQQLVNRLMVNDMQDG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VMLVRANGMVVAANPAAVVLLGVQPHERVYSRRGREGEGDEGGQAALFDLRRIPRLQPLM
EEEEEECCEEEEECCCEEEEECCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHH
EMLRDWLRSKDDVPRILQLLPIVSRPAPPAGGTLHTRLRLRFVLPGLAGLRATVASTSTM
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHEEHHHCCCHHHHHHHHHCCCCC
PSNLDTAAWNELTPEAAARLRLAIAQQEAMAWTPDDEALLRSEMRDTVLVHIESWERIAE
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEHHHHHHHHH
QVQQEKLAAMGRLVASVAHQIRNPLAAISQASELLGDGASMGSGGGVESDVDARLLRIIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
DNVRRLDQVVSDVLQISRRPRTGRSTVHLAQALPEIVDRWRLEMRLRVPRGGDTRQPGRG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCC
EINANAIRLTVDVPQQVVFDTSQLQQVLGNLLDNAWRYCSRLPGAIRLLAHALDQHHAEL
CCCCCEEEEEEECCHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEE
IVWNDGTEIPREHQRSLFEPFFTSNAQGTGLGLFMARELCGANDAQVRYGTIALDDLLAR
EEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHH
TGLLAAPRDTLPAKAFVLTLRIDTGDAAPARGADPLVHRNSASDT
HCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]