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The map label for this gene is pilR [H]

Identifier: 73542627

GI number: 73542627

Start: 3227851

End: 3229440

Strand: Reverse

Name: pilR [H]

Synonym: Reut_A2943

Alternate gene names: 73542627

Gene position: 3229440-3227851 (Counterclockwise)

Preceding gene: 73542628

Following gene: 73542626

Centisome position: 84.84

GC content: 69.18

Gene sequence:

>1590_bases
ATGCCACCCAGAACGCCTGGCCTCGATCCCATCCTCGTCATCGACGACGAGGCAGACTTGCGCGAGCTGCTGGACATTTC
CATCCGCCGCATGGGGCATGACGTGGTGCTGGCCGGCTCGCTGGCCGAAGCGCGCGAGAAGCTCGTACAGCAACGCTACA
GCCTGGTGCTGACCGATATGCGCCTTGGCGACGGCCTCGGTATCGAGATAGTGCGCCAGCTTGCGGCCGCGCCGGAACGT
GTGCCGGTGGCCGTCATCACCGCGTACGGGAGTGCCGACAACGCCGTCGAAGCGCTCAAGGCCGGTGCGTTCGACTACAT
TGCCAAGCCGGTGTCGCTGGACCAGTTGCGCAGCCTCGTGATGAATGCGCTTGGGCGCCAGCAACGCGAAGACCAGGACA
ACGCAGCCGCCGCGGAAGCCGCCCTGGCTGCAGACCGTGCCGCTGCGCTGTTGCCCGGCCACTCGGCGGCCATGCAGGAG
GTGCGCCGTTCGCTGTCGCGGCTCGCGCGCAGCATGGCACCGGTTGTGATCAGCGGCGAGTCGGGCAGCGGCAAGGAACG
CGCCGCGCGCGCCATCCACGCCACCAGCACGCGTGCCGCGCATCCGTTCGTGGCGGTGAACTGTGGCGCCATCCCTGAAA
ACCTGATGGAGTCCGAGTTCTTTGGCTACGTCAAAGGCGCGTTCACCGGCGCCGACGCCGAGCGCAACGGCTTCTTCCAG
GCCGCAAACGGCGGCACGCTGCTGCTCGACGAGGTGGCCGACCTGCCGCTGGCCATGCAGGTCAAGCTGCTGCGCGCGCT
GCAGGAGCGCCGCGTGCGCAAGATCGGCGCGAGCCGCGAAGATGCCGTCGATGTGCGCGTGATGTGCGCGAGCCACAAGG
ACCTGGCCGCGATGGTCGCGGCCGGTCAGTTCCGGCAGGATTTGTACTACCGGCTCAATGTGCTGGGTTTGCGCATGCCG
ACGCTGCGCGAGCGCGCCGAGGACATTCCCGTGCTGGCCCGCGCCATCCTCGAACACCTGGCCGTGCGTTACAGCGACCC
GCGCCCGCGCCGGCTGTCGCAGGCCGCGCTGGAGCGGCTGGTGGCGTATCCGTTTCCGGGCAACGTCCGCGAACTGGAAA
ACCTGCTCGAACGCGCCTACGCGTTTGCCGAGGCCGAGGATATCCAGGTGGAAGACCTGGGGCCGCTCGACGCCAATATG
GAGCACGCGGCGCTGCGTCCCGCGCCGTCGCCGCTTACGCCGTACACGCCATGGGCCGGCGGCCTGGCGCCAGCGGAGCC
CGCTCCGGCACCCGAGCCCACGCCACAGGCCGCCACCGCGCCAGAGTCCGAGGCCATCGTCGTGCCGGCCGACCTGCCTG
ATACCGCCGAACGCGCTTGCCGTGGCGTCACGTTCCCGATCGATCTGCCAGCCCGGCTGGAAGACGTGGAGCGCGAGATC
ATCCTGCAGGCGCTGGCCCAGACCGGCTTCAATCGCACCGCTGCCGCGCCGCTGCTGGGGCTGAACCTGCGCCAGTTGCG
CTACCGCATCCAGCAACTCGGTATCCGCGACGGGCGCGATGAACGCGAGGAAGGGGAGGGCGCGGCATGA

Upstream 100 bases:

>100_bases
GCCTTCGTGCTGACGCTGCGCATCGACACCGGCGATGCGGCGCCGGCCCGAGGCGCCGATCCGCTTGTCCACCGAAACTC
CGCCTCCGATACGTAGCTCC

Downstream 100 bases:

>100_bases
CGGCTCCGGCTGCAGGTCCCATCCCGGCCAGTTTCCTCCCCGACGCCGAAGGCTGGGTTCCGGCCGCGCGCCGCGTGCCG
TCGCCCAACTTCGACGAGCG

Product: helix-turn-helix, Fis-type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MPPRTPGLDPILVIDDEADLRELLDISIRRMGHDVVLAGSLAEAREKLVQQRYSLVLTDMRLGDGLGIEIVRQLAAAPER
VPVAVITAYGSADNAVEALKAGAFDYIAKPVSLDQLRSLVMNALGRQQREDQDNAAAAEAALAADRAAALLPGHSAAMQE
VRRSLSRLARSMAPVVISGESGSGKERAARAIHATSTRAAHPFVAVNCGAIPENLMESEFFGYVKGAFTGADAERNGFFQ
AANGGTLLLDEVADLPLAMQVKLLRALQERRVRKIGASREDAVDVRVMCASHKDLAAMVAAGQFRQDLYYRLNVLGLRMP
TLRERAEDIPVLARAILEHLAVRYSDPRPRRLSQAALERLVAYPFPGNVRELENLLERAYAFAEAEDIQVEDLGPLDANM
EHAALRPAPSPLTPYTPWAGGLAPAEPAPAPEPTPQAATAPESEAIVVPADLPDTAERACRGVTFPIDLPARLEDVEREI
ILQALAQTGFNRTAAAPLLGLNLRQLRYRIQQLGIRDGRDEREEGEGAA

Sequences:

>Translated_529_residues
MPPRTPGLDPILVIDDEADLRELLDISIRRMGHDVVLAGSLAEAREKLVQQRYSLVLTDMRLGDGLGIEIVRQLAAAPER
VPVAVITAYGSADNAVEALKAGAFDYIAKPVSLDQLRSLVMNALGRQQREDQDNAAAAEAALAADRAAALLPGHSAAMQE
VRRSLSRLARSMAPVVISGESGSGKERAARAIHATSTRAAHPFVAVNCGAIPENLMESEFFGYVKGAFTGADAERNGFFQ
AANGGTLLLDEVADLPLAMQVKLLRALQERRVRKIGASREDAVDVRVMCASHKDLAAMVAAGQFRQDLYYRLNVLGLRMP
TLRERAEDIPVLARAILEHLAVRYSDPRPRRLSQAALERLVAYPFPGNVRELENLLERAYAFAEAEDIQVEDLGPLDANM
EHAALRPAPSPLTPYTPWAGGLAPAEPAPAPEPTPQAATAPESEAIVVPADLPDTAERACRGVTFPIDLPARLEDVEREI
ILQALAQTGFNRTAAAPLLGLNLRQLRYRIQQLGIRDGRDEREEGEGAA
>Mature_528_residues
PPRTPGLDPILVIDDEADLRELLDISIRRMGHDVVLAGSLAEAREKLVQQRYSLVLTDMRLGDGLGIEIVRQLAAAPERV
PVAVITAYGSADNAVEALKAGAFDYIAKPVSLDQLRSLVMNALGRQQREDQDNAAAAEAALAADRAAALLPGHSAAMQEV
RRSLSRLARSMAPVVISGESGSGKERAARAIHATSTRAAHPFVAVNCGAIPENLMESEFFGYVKGAFTGADAERNGFFQA
ANGGTLLLDEVADLPLAMQVKLLRALQERRVRKIGASREDAVDVRVMCASHKDLAAMVAAGQFRQDLYYRLNVLGLRMPT
LRERAEDIPVLARAILEHLAVRYSDPRPRRLSQAALERLVAYPFPGNVRELENLLERAYAFAEAEDIQVEDLGPLDANME
HAALRPAPSPLTPYTPWAGGLAPAEPAPAPEPTPQAATAPESEAIVVPADLPDTAERACRGVTFPIDLPARLEDVEREII
LQALAQTGFNRTAAAPLLGLNLRQLRYRIQQLGIRDGRDEREEGEGAA

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=402, Percent_Identity=43.5323383084577, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI1788550, Length=391, Percent_Identity=41.6879795396419, Blast_Score=302, Evalue=4e-83,
Organism=Escherichia coli, GI1788905, Length=375, Percent_Identity=41.0666666666667, Blast_Score=281, Evalue=1e-76,
Organism=Escherichia coli, GI1790299, Length=509, Percent_Identity=35.1669941060904, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1789233, Length=228, Percent_Identity=50, Blast_Score=224, Evalue=2e-59,
Organism=Escherichia coli, GI87082117, Length=265, Percent_Identity=44.5283018867925, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1786524, Length=350, Percent_Identity=40.5714285714286, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI87082152, Length=368, Percent_Identity=38.8586956521739, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1789087, Length=317, Percent_Identity=40.3785488958991, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI87081872, Length=357, Percent_Identity=34.453781512605, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1787583, Length=278, Percent_Identity=38.8489208633094, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1789828, Length=251, Percent_Identity=33.0677290836653, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI87081858, Length=225, Percent_Identity=34.6666666666667, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 57220; Mature: 57089

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPRTPGLDPILVIDDEADLRELLDISIRRMGHDVVLAGSLAEAREKLVQQRYSLVLTDM
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHH
RLGDGLGIEIVRQLAAAPERVPVAVITAYGSADNAVEALKAGAFDYIAKPVSLDQLRSLV
HCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHCCCCCHHHHHHHH
MNALGRQQREDQDNAAAAEAALAADRAAALLPGHSAAMQEVRRSLSRLARSMAPVVISGE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEECC
SGSGKERAARAIHATSTRAAHPFVAVNCGAIPENLMESEFFGYVKGAFTGADAERNGFFQ
CCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEE
AANGGTLLLDEVADLPLAMQVKLLRALQERRVRKIGASREDAVDVRVMCASHKDLAAMVA
ECCCCEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHH
AGQFRQDLYYRLNVLGLRMPTLRERAEDIPVLARAILEHLAVRYSDPRPRRLSQAALERL
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VAYPFPGNVRELENLLERAYAFAEAEDIQVEDLGPLDANMEHAALRPAPSPLTPYTPWAG
HCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
GLAPAEPAPAPEPTPQAATAPESEAIVVPADLPDTAERACRGVTFPIDLPARLEDVEREI
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHH
ILQALAQTGFNRTAAAPLLGLNLRQLRYRIQQLGIRDGRDEREEGEGAA
HHHHHHHCCCCCHHHCHHHCCCHHHHHHHHHHCCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
PPRTPGLDPILVIDDEADLRELLDISIRRMGHDVVLAGSLAEAREKLVQQRYSLVLTDM
CCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHH
RLGDGLGIEIVRQLAAAPERVPVAVITAYGSADNAVEALKAGAFDYIAKPVSLDQLRSLV
HCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHCCCCCHHHHHHHH
MNALGRQQREDQDNAAAAEAALAADRAAALLPGHSAAMQEVRRSLSRLARSMAPVVISGE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEECC
SGSGKERAARAIHATSTRAAHPFVAVNCGAIPENLMESEFFGYVKGAFTGADAERNGFFQ
CCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEE
AANGGTLLLDEVADLPLAMQVKLLRALQERRVRKIGASREDAVDVRVMCASHKDLAAMVA
ECCCCEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHH
AGQFRQDLYYRLNVLGLRMPTLRERAEDIPVLARAILEHLAVRYSDPRPRRLSQAALERL
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VAYPFPGNVRELENLLERAYAFAEAEDIQVEDLGPLDANMEHAALRPAPSPLTPYTPWAG
HCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
GLAPAEPAPAPEPTPQAATAPESEAIVVPADLPDTAERACRGVTFPIDLPARLEDVEREI
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHH
ILQALAQTGFNRTAAAPLLGLNLRQLRYRIQQLGIRDGRDEREEGEGAA
HHHHHHHCCCCCHHHCHHHCCCHHHHHHHHHHCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]