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The map label for this gene is ampD [H]

Identifier: 73542626

GI number: 73542626

Start: 3227243

End: 3227854

Strand: Reverse

Name: ampD [H]

Synonym: Reut_A2942

Alternate gene names: 73542626

Gene position: 3227854-3227243 (Counterclockwise)

Preceding gene: 73542627

Following gene: 73542625

Centisome position: 84.8

GC content: 66.99

Gene sequence:

>612_bases
ATGACGGCTCCGGCTGCAGGTCCCATCCCGGCCAGTTTCCTCCCCGACGCCGAAGGCTGGGTTCCGGCCGCGCGCCGCGT
GCCGTCGCCCAACTTCGACGAGCGGCCCGCCGGCATGCCGGTGGACCTGGTGGTGCTCCATAACATCAGCCTGCCGCCGG
GCCAGTTCGGCAGCGGTGATATCGAGGCCTTCTTCCAGAACCGGCTCGACCCCGACCGCCACCCGTTCTTCGCGACGATC
CATCAGGTGCAGGTATCGGCGCACTTCCTGGTGCGGCGCGATGGTGAACTCGTGCAGTTCGTCCCGTGCACCAAGCGCGC
CTGGCATGCCGGCCAGTCCGATTTCTTCGGCCGCAGCCGTTGCAATGACTTCTCGGTCGGCATCGAGATCGAAGGCACTG
ACGACTTGCCATTCACCGCCGCCCAGTACACCACCACCTCCACGCTGGTGAAGGCCCTGCGCGCCACCCTGCCAGTGCGG
GCGGTTGCAGGCCATAGCGACATCGCTCCGGGCCGCAAGACCGACCCCGGACCGCACTTCGACTGGGACCGTTTTGCCGG
TCTCGCCGGGGTTCCGCAAAACCTGTTACCCTACCGCCCCTCGCACAACTAA

Upstream 100 bases:

>100_bases
CGCCGCTGCTGGGGCTGAACCTGCGCCAGTTGCGCTACCGCATCCAGCAACTCGGTATCCGCGACGGGCGCGATGAACGC
GAGGAAGGGGAGGGCGCGGC

Downstream 100 bases:

>100_bases
GGGGCTGTCGGGCAGCCGCCACAGCACCATGTCGGCGCGGTGTCACATGCCAATTCCCCAGGGTGGTGTGGGGCTATCAG
GCGCGCCACACGGATAAAAA

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MTAPAAGPIPASFLPDAEGWVPAARRVPSPNFDERPAGMPVDLVVLHNISLPPGQFGSGDIEAFFQNRLDPDRHPFFATI
HQVQVSAHFLVRRDGELVQFVPCTKRAWHAGQSDFFGRSRCNDFSVGIEIEGTDDLPFTAAQYTTTSTLVKALRATLPVR
AVAGHSDIAPGRKTDPGPHFDWDRFAGLAGVPQNLLPYRPSHN

Sequences:

>Translated_203_residues
MTAPAAGPIPASFLPDAEGWVPAARRVPSPNFDERPAGMPVDLVVLHNISLPPGQFGSGDIEAFFQNRLDPDRHPFFATI
HQVQVSAHFLVRRDGELVQFVPCTKRAWHAGQSDFFGRSRCNDFSVGIEIEGTDDLPFTAAQYTTTSTLVKALRATLPVR
AVAGHSDIAPGRKTDPGPHFDWDRFAGLAGVPQNLLPYRPSHN
>Mature_202_residues
TAPAAGPIPASFLPDAEGWVPAARRVPSPNFDERPAGMPVDLVVLHNISLPPGQFGSGDIEAFFQNRLDPDRHPFFATIH
QVQVSAHFLVRRDGELVQFVPCTKRAWHAGQSDFFGRSRCNDFSVGIEIEGTDDLPFTAAQYTTTSTLVKALRATLPVRA
VAGHSDIAPGRKTDPGPHFDWDRFAGLAGVPQNLLPYRPSHN

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=174, Percent_Identity=56.8965517241379, Blast_Score=203, Evalue=8e-54,
Organism=Escherichia coli, GI1787092, Length=128, Percent_Identity=39.84375, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 22161; Mature: 22030

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPAAGPIPASFLPDAEGWVPAARRVPSPNFDERPAGMPVDLVVLHNISLPPGQFGSGD
CCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCH
IEAFFQNRLDPDRHPFFATIHQVQVSAHFLVRRDGELVQFVPCTKRAWHAGQSDFFGRSR
HHHHHHHCCCCCCCCHHHHHHHHHHHEEEEEECCCCEEEEECCCHHHHCCCCCCCCCCCC
CNDFSVGIEIEGTDDLPFTAAQYTTTSTLVKALRATLPVRAVAGHSDIAPGRKTDPGPHF
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCCCCCCC
DWDRFAGLAGVPQNLLPYRPSHN
CHHHHCCCCCCCHHHCCCCCCCC
>Mature Secondary Structure 
TAPAAGPIPASFLPDAEGWVPAARRVPSPNFDERPAGMPVDLVVLHNISLPPGQFGSGD
CCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCH
IEAFFQNRLDPDRHPFFATIHQVQVSAHFLVRRDGELVQFVPCTKRAWHAGQSDFFGRSR
HHHHHHHCCCCCCCCHHHHHHHHHHHEEEEEECCCCEEEEECCCHHHHCCCCCCCCCCCC
CNDFSVGIEIEGTDDLPFTAAQYTTTSTLVKALRATLPVRAVAGHSDIAPGRKTDPGPHF
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCCCCCCC
DWDRFAGLAGVPQNLLPYRPSHN
CHHHHCCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 8419294 [H]