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The map label for this gene is gcvA [H]

Identifier: 73542473

GI number: 73542473

Start: 3058611

End: 3059501

Strand: Direct

Name: gcvA [H]

Synonym: Reut_A2788

Alternate gene names: 73542473

Gene position: 3058611-3059501 (Clockwise)

Preceding gene: 73542472

Following gene: 73542474

Centisome position: 80.35

GC content: 66.44

Gene sequence:

>891_bases
ATGGCTTCGATGTTCAACCGCGTCCCGCCTCTGCACCTGCTCGTGGCCTTTGAAGCCGCGGCGCGCCTGGGCAGTTTCGC
GCGCGCGGCCGAGGAGTTGTCGGTCACGCCCAGCGCGGTGTCGCACCGCATCAAGAACCTGGAAGAGCTCTGGGGCGAGG
ATCTGTTCGTGCGCTCGAACGCCGCGCTGCGCCTGACTGCGGCCGGCACGCGTTATCTGCGCAACGTGCAGGACGCGCTC
AAATCGCTCAACGAACTGGCGCGGCCCGAGTACAACAAGATGCGCACGCGGCTGCGCGTGGCGATCCCACCGACGTTCGG
GCGCCAGCACCTGGTGCCGCGCTTGCCCGAGTTCGGCGCGCTGTATCCGCATATCGACATCGAGCTGCACCTGGCCATCC
CGTTCCTAGACGTGAAGGCCGAAGACACCGATGTCGAGATCCGCTACGGAACGGGCCGCTATCCGGACCTGAAGACGACC
AAGCTGCTGGTCGAGCCCGTGTTCCCGGCCTGCGGGCGCGAGTACTACGAGCGCGTCAACGGCCGCGCCATCACCAAGCC
CGAGCATCTGCACGGACTGGTGCTGCTGCGCAGTCCGCTGGAACCGTGGAAGCCGTGGTTCGAAACCGCCGGCCTGGACT
GGCCCGAGCCGCAGACCGGTCCGCAGCTCAACGATATCGGCCTGATGCTGGAAGCGGTCGCCTCCAACCAGGGCGTGGCG
CTTGTGCGCCAGCGCATGGCGCGGCACTGGCTGTCGCTGGGCCAGATGGTAAGGCTGCTCGATATCGAATCGGTGTCGCC
GCACGGCTACTACATCGTCGAGCGCGAACAGGCGCCACTCAAGCCCGAAGCGCGCTACTTCGTCGACTGGCTCTTGAGCC
TGGACTGGTAA

Upstream 100 bases:

>100_bases
TGGACCTGATGCGCGTGATCAAGCACGCGCTCGATCCGAACGGCATCCTGAATCCCGGCAAGATCTTCCGCTCCGCGCGC
GGCCCGGCGGGCTGAGCGGC

Downstream 100 bases:

>100_bases
GGAAGAGACTCGCCATGGAACTGCGCCTGTTGACCCCCACCGATGCTCTCGCTTTCCAGGCCTTGCGCCTGGCCGGGTTG
GTCGAGGCGCCCGAGGCATT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MASMFNRVPPLHLLVAFEAAARLGSFARAAEELSVTPSAVSHRIKNLEELWGEDLFVRSNAALRLTAAGTRYLRNVQDAL
KSLNELARPEYNKMRTRLRVAIPPTFGRQHLVPRLPEFGALYPHIDIELHLAIPFLDVKAEDTDVEIRYGTGRYPDLKTT
KLLVEPVFPACGREYYERVNGRAITKPEHLHGLVLLRSPLEPWKPWFETAGLDWPEPQTGPQLNDIGLMLEAVASNQGVA
LVRQRMARHWLSLGQMVRLLDIESVSPHGYYIVEREQAPLKPEARYFVDWLLSLDW

Sequences:

>Translated_296_residues
MASMFNRVPPLHLLVAFEAAARLGSFARAAEELSVTPSAVSHRIKNLEELWGEDLFVRSNAALRLTAAGTRYLRNVQDAL
KSLNELARPEYNKMRTRLRVAIPPTFGRQHLVPRLPEFGALYPHIDIELHLAIPFLDVKAEDTDVEIRYGTGRYPDLKTT
KLLVEPVFPACGREYYERVNGRAITKPEHLHGLVLLRSPLEPWKPWFETAGLDWPEPQTGPQLNDIGLMLEAVASNQGVA
LVRQRMARHWLSLGQMVRLLDIESVSPHGYYIVEREQAPLKPEARYFVDWLLSLDW
>Mature_295_residues
ASMFNRVPPLHLLVAFEAAARLGSFARAAEELSVTPSAVSHRIKNLEELWGEDLFVRSNAALRLTAAGTRYLRNVQDALK
SLNELARPEYNKMRTRLRVAIPPTFGRQHLVPRLPEFGALYPHIDIELHLAIPFLDVKAEDTDVEIRYGTGRYPDLKTTK
LLVEPVFPACGREYYERVNGRAITKPEHLHGLVLLRSPLEPWKPWFETAGLDWPEPQTGPQLNDIGLMLEAVASNQGVAL
VRQRMARHWLSLGQMVRLLDIESVSPHGYYIVEREQAPLKPEARYFVDWLLSLDW

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=34.5890410958904, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=32.8719723183391, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1788706, Length=286, Percent_Identity=30.0699300699301, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI157672245, Length=229, Percent_Identity=31.8777292576419, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=26.8965517241379, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786401, Length=304, Percent_Identity=27.3026315789474, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=25.4295532646048, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790262, Length=246, Percent_Identity=26.8292682926829, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87081978, Length=162, Percent_Identity=30.8641975308642, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33744; Mature: 33613

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASMFNRVPPLHLLVAFEAAARLGSFARAAEELSVTPSAVSHRIKNLEELWGEDLFVRSN
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECC
AALRLTAAGTRYLRNVQDALKSLNELARPEYNKMRTRLRVAIPPTFGRQHLVPRLPEFGA
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEECCCCCCCCCCCCCCCCCC
LYPHIDIELHLAIPFLDVKAEDTDVEIRYGTGRYPDLKTTKLLVEPVFPACGREYYERVN
CCCCEEEEEEEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHHHC
GRAITKPEHLHGLVLLRSPLEPWKPWFETAGLDWPEPQTGPQLNDIGLMLEAVASNQGVA
CCCCCCCHHHHEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHH
LVRQRMARHWLSLGQMVRLLDIESVSPHGYYIVEREQAPLKPEARYFVDWLLSLDW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASMFNRVPPLHLLVAFEAAARLGSFARAAEELSVTPSAVSHRIKNLEELWGEDLFVRSN
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECC
AALRLTAAGTRYLRNVQDALKSLNELARPEYNKMRTRLRVAIPPTFGRQHLVPRLPEFGA
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEECCCCCCCCCCCCCCCCCC
LYPHIDIELHLAIPFLDVKAEDTDVEIRYGTGRYPDLKTTKLLVEPVFPACGREYYERVN
CCCCEEEEEEEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHHHC
GRAITKPEHLHGLVLLRSPLEPWKPWFETAGLDWPEPQTGPQLNDIGLMLEAVASNQGVA
CCCCCCCHHHHEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHH
LVRQRMARHWLSLGQMVRLLDIESVSPHGYYIVEREQAPLKPEARYFVDWLLSLDW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]