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The map label for this gene is glcD [H]

Identifier: 73542472

GI number: 73542472

Start: 3057175

End: 3058605

Strand: Direct

Name: glcD [H]

Synonym: Reut_A2787

Alternate gene names: 73542472

Gene position: 3057175-3058605 (Clockwise)

Preceding gene: 73542467

Following gene: 73542473

Centisome position: 80.31

GC content: 67.71

Gene sequence:

>1431_bases
ATGAACCACCCCGCGCCACACGCCGCCCTGGCTCGCCGCCCGTTGCCTTCTGCATTGCGTGACGCGCTGCAGGCACGCTT
CGGCGACCGTTTCTCGACCTCCGCCGGCGTCTGCGAACACCATGGCCGCGACGAATCGCCGTTCCCGCCGGCGCTCCCGG
ACGGCGTGGTGTATGCGCACAGTACCGAGGAAGTGGCCGAGGTTGCGCGTCTGTGCAACGAGCACGGCGTGCCGTTGATC
CCCTACGGTGCGGGCTCCTCGCTCGAAGGCCACCTGCTGGCCGTGGCCGGCGGCATCAGCCTGGACCTCTCGCAGATGAA
CAAGGTGCTGGCGATCCAGCCGGAAGACCTGACCGTCACCGTGCAGCCCGGCGTCACGCGCAAGCAGCTCAACCAGGACA
TCAAGGACACGGGCCTGTTTTTCCCGATTGACCCGGGCGCGGATGCGTCGCTGGGCGGCATGTGCGCGACGCGGGCCTCG
GGGACCAATGCAGTGCGCTACGGCACGATGCGCGAGAACGTGCTCGCGCTGACCGTAGTGACCGCCGACGGGCGCGTGAT
CCACACCGGCACGCATGCGCGCAAGTCGTCGGCGGGCTATGACCTGACGCGCCTGTTTATCGGTAGCGAAGGCACCCTCG
GCATCATCACCGAGGTAACGGTGCGCCTGTACCCGCAGCCGGAAGCGATTTCCGCCGCGGTCTGCGCATTCCCAAGCATG
AGCAGCGCCGTGCAGGCCGTGATCCAGACCATTCAGCTCGGCGTGCCGATCGCGCGCGTGGAGTTCGTCGATGCGCTGGC
CATCCGCGCGATCAACCGGCACGACAACCTGACGCTGCCCGAAATGCCGCATCTGTTCTTCGAGTTCCACGGCACCGAGG
CCGGCGTGCGCGAACAGGCCGAGACCGTGCAGCAGATCACCACCGAGCATGGCGGCCAGGACTTCGAGTGGGCCACGCGG
CCCGAAGACCGCAGCCGTCTGTGGAACGCTCGCCATACCGCGTATTTCGCGATGCTGCAGCTCAAGCCGGGCTGCCGCGC
AGTGACCACCGACGTGTGCGTGCCGATCTCGCGCCTGGCCGACTGCGTGGCCGAGACCGAGAAAGACCTGATCGCCTCGT
CGCTGCCCTGCCCGATCGTCGGGCACGTCGGCGACGGCAATTTCCACGTCGCGATCCTGATCGACCCCGACAAGCCCGAA
GAGATGGCCGAGGCCGAGGACATCAACCGCCGCATCGTCGAGCGCGCGCTGGCGATGGGCGGCACCTGCACCGGCGAACA
TGGCGTCGGCCTGCACAAGATGGATTTCCTGGTGCAGGAGCATGGCGAGGACGCGCTGGACCTGATGCGCGTGATCAAGC
ACGCGCTCGATCCGAACGGCATCCTGAATCCCGGCAAGATCTTCCGCTCCGCGCGCGGCCCGGCGGGCTGA

Upstream 100 bases:

>100_bases
TCAACAACCGGATCGCGCCCCTTCCGTCGCCGACGCCGCCGACAACCGCCGACGGTTCCGGAAGCCTGCCGCATCGCGCA
GCGACCCGCCGGAGACCCGC

Downstream 100 bases:

>100_bases
GCGGCATGGCTTCGATGTTCAACCGCGTCCCGCCTCTGCACCTGCTCGTGGCCTTTGAAGCCGCGGCGCGCCTGGGCAGT
TTCGCGCGCGCGGCCGAGGA

Product: FAD linked oxidase, C-terminal:FAD linked oxidase, N-terminal

Products: pyruvate; ferrocytochrome c

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MNHPAPHAALARRPLPSALRDALQARFGDRFSTSAGVCEHHGRDESPFPPALPDGVVYAHSTEEVAEVARLCNEHGVPLI
PYGAGSSLEGHLLAVAGGISLDLSQMNKVLAIQPEDLTVTVQPGVTRKQLNQDIKDTGLFFPIDPGADASLGGMCATRAS
GTNAVRYGTMRENVLALTVVTADGRVIHTGTHARKSSAGYDLTRLFIGSEGTLGIITEVTVRLYPQPEAISAAVCAFPSM
SSAVQAVIQTIQLGVPIARVEFVDALAIRAINRHDNLTLPEMPHLFFEFHGTEAGVREQAETVQQITTEHGGQDFEWATR
PEDRSRLWNARHTAYFAMLQLKPGCRAVTTDVCVPISRLADCVAETEKDLIASSLPCPIVGHVGDGNFHVAILIDPDKPE
EMAEAEDINRRIVERALAMGGTCTGEHGVGLHKMDFLVQEHGEDALDLMRVIKHALDPNGILNPGKIFRSARGPAG

Sequences:

>Translated_476_residues
MNHPAPHAALARRPLPSALRDALQARFGDRFSTSAGVCEHHGRDESPFPPALPDGVVYAHSTEEVAEVARLCNEHGVPLI
PYGAGSSLEGHLLAVAGGISLDLSQMNKVLAIQPEDLTVTVQPGVTRKQLNQDIKDTGLFFPIDPGADASLGGMCATRAS
GTNAVRYGTMRENVLALTVVTADGRVIHTGTHARKSSAGYDLTRLFIGSEGTLGIITEVTVRLYPQPEAISAAVCAFPSM
SSAVQAVIQTIQLGVPIARVEFVDALAIRAINRHDNLTLPEMPHLFFEFHGTEAGVREQAETVQQITTEHGGQDFEWATR
PEDRSRLWNARHTAYFAMLQLKPGCRAVTTDVCVPISRLADCVAETEKDLIASSLPCPIVGHVGDGNFHVAILIDPDKPE
EMAEAEDINRRIVERALAMGGTCTGEHGVGLHKMDFLVQEHGEDALDLMRVIKHALDPNGILNPGKIFRSARGPAG
>Mature_476_residues
MNHPAPHAALARRPLPSALRDALQARFGDRFSTSAGVCEHHGRDESPFPPALPDGVVYAHSTEEVAEVARLCNEHGVPLI
PYGAGSSLEGHLLAVAGGISLDLSQMNKVLAIQPEDLTVTVQPGVTRKQLNQDIKDTGLFFPIDPGADASLGGMCATRAS
GTNAVRYGTMRENVLALTVVTADGRVIHTGTHARKSSAGYDLTRLFIGSEGTLGIITEVTVRLYPQPEAISAAVCAFPSM
SSAVQAVIQTIQLGVPIARVEFVDALAIRAINRHDNLTLPEMPHLFFEFHGTEAGVREQAETVQQITTEHGGQDFEWATR
PEDRSRLWNARHTAYFAMLQLKPGCRAVTTDVCVPISRLADCVAETEKDLIASSLPCPIVGHVGDGNFHVAILIDPDKPE
EMAEAEDINRRIVERALAMGGTCTGEHGVGLHKMDFLVQEHGEDALDLMRVIKHALDPNGILNPGKIFRSARGPAG

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=444, Percent_Identity=52.027027027027, Blast_Score=487, Evalue=1e-137,
Organism=Homo sapiens, GI37595754, Length=467, Percent_Identity=49.4646680942184, Blast_Score=474, Evalue=1e-133,
Organism=Homo sapiens, GI119964728, Length=420, Percent_Identity=32.8571428571429, Blast_Score=195, Evalue=8e-50,
Organism=Homo sapiens, GI4501993, Length=458, Percent_Identity=24.6724890829694, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1789351, Length=415, Percent_Identity=32.7710843373494, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI1787977, Length=494, Percent_Identity=28.3400809716599, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI48994907, Length=501, Percent_Identity=25.9481037924152, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI48994914, Length=189, Percent_Identity=29.1005291005291, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71992373, Length=441, Percent_Identity=48.5260770975057, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71992381, Length=441, Percent_Identity=47.3922902494331, Blast_Score=413, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI71992392, Length=381, Percent_Identity=50.3937007874016, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17534361, Length=422, Percent_Identity=25.1184834123223, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17556096, Length=202, Percent_Identity=31.6831683168317, Blast_Score=111, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6320027, Length=435, Percent_Identity=40.2298850574713, Blast_Score=326, Evalue=5e-90,
Organism=Saccharomyces cerevisiae, GI6320764, Length=437, Percent_Identity=28.8329519450801, Blast_Score=169, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6320023, Length=432, Percent_Identity=29.1666666666667, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI18921117, Length=420, Percent_Identity=28.5714285714286, Blast_Score=162, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24639277, Length=420, Percent_Identity=28.5714285714286, Blast_Score=162, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24639275, Length=420, Percent_Identity=28.5714285714286, Blast_Score=162, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24653753, Length=458, Percent_Identity=26.2008733624454, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.2.4

Molecular weight: Translated: 51153; Mature: 51153

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHPAPHAALARRPLPSALRDALQARFGDRFSTSAGVCEHHGRDESPFPPALPDGVVYAH
CCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEC
STEEVAEVARLCNEHGVPLIPYGAGSSLEGHLLAVAGGISLDLSQMNKVLAIQPEDLTVT
CHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCEEEHHHCCCEEEECCCCEEEE
VQPGVTRKQLNQDIKDTGLFFPIDPGADASLGGMCATRASGTNAVRYGTMRENVLALTVV
ECCCCCHHHHCCCHHHCCCEEEECCCCCCCCCCEEEECCCCCCEEEECCCCCCEEEEEEE
TADGRVIHTGTHARKSSAGYDLTRLFIGSEGTLGIITEVTVRLYPQPEAISAAVCAFPSM
ECCCEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHHCCCH
SSAVQAVIQTIQLGVPIARVEFVDALAIRAINRHDNLTLPEMPHLFFEFHGTEAGVREQA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHH
ETVQQITTEHGGQDFEWATRPEDRSRLWNARHTAYFAMLQLKPGCRAVTTDVCVPISRLA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHEEEEEEEECCCCCEEHHHHHHHHHHHH
DCVAETEKDLIASSLPCPIVGHVGDGNFHVAILIDPDKPEEMAEAEDINRRIVERALAMG
HHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
GTCTGEHGVGLHKMDFLVQEHGEDALDLMRVIKHALDPNGILNPGKIFRSARGPAG
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MNHPAPHAALARRPLPSALRDALQARFGDRFSTSAGVCEHHGRDESPFPPALPDGVVYAH
CCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEC
STEEVAEVARLCNEHGVPLIPYGAGSSLEGHLLAVAGGISLDLSQMNKVLAIQPEDLTVT
CHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCEEEHHHCCCEEEECCCCEEEE
VQPGVTRKQLNQDIKDTGLFFPIDPGADASLGGMCATRASGTNAVRYGTMRENVLALTVV
ECCCCCHHHHCCCHHHCCCEEEECCCCCCCCCCEEEECCCCCCEEEECCCCCCEEEEEEE
TADGRVIHTGTHARKSSAGYDLTRLFIGSEGTLGIITEVTVRLYPQPEAISAAVCAFPSM
ECCCEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHHCCCH
SSAVQAVIQTIQLGVPIARVEFVDALAIRAINRHDNLTLPEMPHLFFEFHGTEAGVREQA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHH
ETVQQITTEHGGQDFEWATRPEDRSRLWNARHTAYFAMLQLKPGCRAVTTDVCVPISRLA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHEEEEEEEECCCCCEEHHHHHHHHHHHH
DCVAETEKDLIASSLPCPIVGHVGDGNFHVAILIDPDKPEEMAEAEDINRRIVERALAMG
HHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
GTCTGEHGVGLHKMDFLVQEHGEDALDLMRVIKHALDPNGILNPGKIFRSARGPAG
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; ferricytochrome c

Specific reaction: (R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]