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The map label for this gene is lspA

Identifier: 73542431

GI number: 73542431

Start: 3016488

End: 3017018

Strand: Direct

Name: lspA

Synonym: Reut_A2746

Alternate gene names: 73542431

Gene position: 3016488-3017018 (Clockwise)

Preceding gene: 73542430

Following gene: 73542432

Centisome position: 79.25

GC content: 63.84

Gene sequence:

>531_bases
ATGGCATCGACCACGTCCCGTTCCGCCCGCCCCGCGCGGCGCAATAACAAGGCGTCGGGCAACACCACGCCACTGCTGTG
GATGGCGTTCGCCCTGCTGGTGGTGGTGCTGGACCAGTTCTTCAAGATCGTCATCGTGCGTACGTTTACGTACGGCGAAT
CGCGCCCGGTGACGCGCTTTTTTAACCTGGTCCTGGTGTACAACAAGGGCGCAGCGTTCAGCTTCCTGGCCGACGCCGGC
GGCTGGCAGCGCTGGTTCTTCACCGGGCTCGGCCTCGTGGTCGGCGCGTTCATCGTCTGGCTGCTGTACCGCCATACGGG
TCAGAAGCTGTTCTGCTTCGCTGTGTCGCTGATCCTCGGCGGCGCAGTGGGCAATGTGGTCGACCGCGTGGTCTACGGCC
ACGTGATCGACTTCCTCGATTTCTACGTGCGCAACTACCACTGGCCGGCGTTCAACGTGGCCGACTGCGCCATTACCGTG
GGTGCGGTGCTGCTGATCGTCGACGAGCTGCGGCGCGTGCGCAAGCACTAA

Upstream 100 bases:

>100_bases
ACTACCGCGCCGACGTCGGACAGAATCCGGACCACCCGACCCTCTGCGGCCGCTGCGACAGCAATCTGTTCGGCGCCGGT
GAACACAGGAGCCATGCCTG

Downstream 100 bases:

>100_bases
GCCGCCGCCGGCAGCCTGCGCAGGCTGCCGGTATCATGCCGAAACCCAATCTCTGACGAGCCTTCCATGGATTTGCGCGG
CAAGCACATCGTTCTCGGCC

Product: lipoprotein signal peptidase

Products: NA

Alternate protein names: Prolipoprotein signal peptidase; Signal peptidase II; SPase II

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MASTTSRSARPARRNNKASGNTTPLLWMAFALLVVVLDQFFKIVIVRTFTYGESRPVTRFFNLVLVYNKGAAFSFLADAG
GWQRWFFTGLGLVVGAFIVWLLYRHTGQKLFCFAVSLILGGAVGNVVDRVVYGHVIDFLDFYVRNYHWPAFNVADCAITV
GAVLLIVDELRRVRKH

Sequences:

>Translated_176_residues
MASTTSRSARPARRNNKASGNTTPLLWMAFALLVVVLDQFFKIVIVRTFTYGESRPVTRFFNLVLVYNKGAAFSFLADAG
GWQRWFFTGLGLVVGAFIVWLLYRHTGQKLFCFAVSLILGGAVGNVVDRVVYGHVIDFLDFYVRNYHWPAFNVADCAITV
GAVLLIVDELRRVRKH
>Mature_175_residues
ASTTSRSARPARRNNKASGNTTPLLWMAFALLVVVLDQFFKIVIVRTFTYGESRPVTRFFNLVLVYNKGAAFSFLADAGG
WQRWFFTGLGLVVGAFIVWLLYRHTGQKLFCFAVSLILGGAVGNVVDRVVYGHVIDFLDFYVRNYHWPAFNVADCAITVG
AVLLIVDELRRVRKH

Specific function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins

COG id: COG0597

COG function: function code MU; Lipoprotein signal peptidase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A8 family

Homologues:

Organism=Escherichia coli, GI1786210, Length=158, Percent_Identity=41.7721518987342, Blast_Score=128, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LSPA_CUPPJ (Q46XM6)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296951.1
- MEROPS:   A08.001
- GeneID:   3612452
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A2746
- NMPDR:   fig|264198.3.peg.2179
- HOGENOM:   HBG724422
- OMA:   VIICDQL
- ProtClustDB:   PRK00376
- BioCyc:   REUT264198:REUT_A2746-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_00161
- InterPro:   IPR001872
- PRINTS:   PR00781
- TIGRFAMs:   TIGR00077

Pfam domain/function: PF01252 Peptidase_A8

EC number: =3.4.23.36

Molecular weight: Translated: 19826; Mature: 19695

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: PS00855 SPASE_II

Important sites: ACT_SITE 128-128 ACT_SITE 155-155

Signals:

None

Transmembrane regions:

HASH(0x11423e04)-; HASH(0x406ee0f8)-; HASH(0x11415b90)-; HASH(0x12a72584)-; HASH(0x406ee890)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTTSRSARPARRNNKASGNTTPLLWMAFALLVVVLDQFFKIVIVRTFTYGESRPVTRF
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
FNLVLVYNKGAAFSFLADAGGWQRWFFTGLGLVVGAFIVWLLYRHTGQKLFCFAVSLILG
HHHHHEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GAVGNVVDRVVYGHVIDFLDFYVRNYHWPAFNVADCAITVGAVLLIVDELRRVRKH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
ASTTSRSARPARRNNKASGNTTPLLWMAFALLVVVLDQFFKIVIVRTFTYGESRPVTRF
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
FNLVLVYNKGAAFSFLADAGGWQRWFFTGLGLVVGAFIVWLLYRHTGQKLFCFAVSLILG
HHHHHEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GAVGNVVDRVVYGHVIDFLDFYVRNYHWPAFNVADCAITVGAVLLIVDELRRVRKH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA