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The map label for this gene is sucB [H]

Identifier: 73541734

GI number: 73541734

Start: 2245791

End: 2247050

Strand: Reverse

Name: sucB [H]

Synonym: Reut_A2046

Alternate gene names: 73541734

Gene position: 2247050-2245791 (Counterclockwise)

Preceding gene: 73541735

Following gene: 73541733

Centisome position: 59.03

GC content: 65.71

Gene sequence:

>1260_bases
ATGGCTATTGTTGACGTCAAGGTTCCGCAACTGTCCGAATCGGTCGCCGAAGCGACCATGCTGAACTGGAAGAAGAAGCC
TGGCGAAGCCGTCGCCCAGGACGAGATCCTGATCGAGATCGAGACCGACAAGGTCGTGCTGGAAGTGCCGGCACCGTCGG
CCGGCGTGCTGTCGCAGATCATCAAGAACGACGGCGACACCGTCGTGGCCGATGAACTGATCGCCAAGATCGACACCGAA
GCCACCGCTGGCGCTGCGGCTCCCGCCGCCGCCGCACCGGCTCCGGCCGCTGCCGCACCGGCTGCTGCCGCCGCTGCTGC
GCCGGCCGCCGCTGGCGCAGTCGCCATGCCGTCGGCTGCCAAGCTGATGGCCGAAGCCGGCCTGTCGGCTGGCCAGGTCG
CCGGCACCGGCAAGGATGGCCGCATCACCAAGGGCGACGTGCTGGGCGCTTCGGCTGCTCCGGCACCCGCACCGGCCGCC
AAGGCGGCGCCGGCACCCGCCGCCGCCAAGCCGGCCCTGCCGCAAGTTGCTGCCAATGTGGACTTCGCCGCGCTGGGCGA
CCGTCCGGAAGAGCGCGTGCCGATGAGCCGCCTGCGCGCCCGTATCGCCGAGCGCCTGGTGCAGTCGCAATCGACCAACG
CCATCCTCACCACCTTCAATGAAGTGAACATGAAGCCGGTGATGGATCTGCGCAACAAGTACAAGGACCGCTTCGAGAAG
GAACACGGCGTGAAGCTCGGCTTCATGTCGTTCTTCGTGAAGGCCGCTGTCCACGCGCTGAAGAAGTACCCGGTCATCAA
CGCGTCGGTCGACGGCAACGACATCGTTTACCACGGTTACTTCGACATCGGTATCGCTGTCGGCTCGCCGCGTGGCCTGG
TGGTGCCGATTCTGCGCAACGCCGACCAGATGAGCCTGGCCGACATCGAGAAGAAGATCGCCGAGTTCGGCGCCAAGGCC
CGCGACGGCAAGCTGTCGCTGGAAGAACTGACCGGCGGTACGTTCTCAATCTCGAACGGCGGCACGTTCGGTTCGATGCT
GTCGACTCCGATCATCAACCCGCCGCAATCGGCCATCCTGGGCGTGCACGCGACCAAGGATCGCGCCGTGGTGGAAGACG
GCCAGATCGTGATCCGTCCGATGAACTACCTGGCCATGTCGTATGACCACCGCATCATCGATGGCCGCGAAGCCGTGCTG
GGCCTGGTGGCGATGAAGGAAGCGCTGGAAGATCCGGCCCGCCTGCTGCTGGACCTGTAA

Upstream 100 bases:

>100_bases
TCCTGACCAAGTAAATGTGGCAGCACGGCGGCGCTCGCAGGTGACCGCCGTGTTCTTTCCTGCCTGAAGAACAAAACGCA
TACACGAGGAATTCACAACC

Downstream 100 bases:

>100_bases
GAACCGGTTTCCCCGCGCGCTGCCGCAATCGGTCGCGCGGGGCGTGTTCCCAGTGTTGAGGGAGTGCCGGCACAAGCTGC
TGCCGGCGCCCAGAAAGGAA

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MAIVDVKVPQLSESVAEATMLNWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLSQIIKNDGDTVVADELIAKIDTE
ATAGAAAPAAAAPAPAAAAPAAAAAAAPAAAGAVAMPSAAKLMAEAGLSAGQVAGTGKDGRITKGDVLGASAAPAPAPAA
KAAPAPAAAKPALPQVAANVDFAALGDRPEERVPMSRLRARIAERLVQSQSTNAILTTFNEVNMKPVMDLRNKYKDRFEK
EHGVKLGFMSFFVKAAVHALKKYPVINASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSLADIEKKIAEFGAKA
RDGKLSLEELTGGTFSISNGGTFGSMLSTPIINPPQSAILGVHATKDRAVVEDGQIVIRPMNYLAMSYDHRIIDGREAVL
GLVAMKEALEDPARLLLDL

Sequences:

>Translated_419_residues
MAIVDVKVPQLSESVAEATMLNWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLSQIIKNDGDTVVADELIAKIDTE
ATAGAAAPAAAAPAPAAAAPAAAAAAAPAAAGAVAMPSAAKLMAEAGLSAGQVAGTGKDGRITKGDVLGASAAPAPAPAA
KAAPAPAAAKPALPQVAANVDFAALGDRPEERVPMSRLRARIAERLVQSQSTNAILTTFNEVNMKPVMDLRNKYKDRFEK
EHGVKLGFMSFFVKAAVHALKKYPVINASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSLADIEKKIAEFGAKA
RDGKLSLEELTGGTFSISNGGTFGSMLSTPIINPPQSAILGVHATKDRAVVEDGQIVIRPMNYLAMSYDHRIIDGREAVL
GLVAMKEALEDPARLLLDL
>Mature_418_residues
AIVDVKVPQLSESVAEATMLNWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLSQIIKNDGDTVVADELIAKIDTEA
TAGAAAPAAAAPAPAAAAPAAAAAAAPAAAGAVAMPSAAKLMAEAGLSAGQVAGTGKDGRITKGDVLGASAAPAPAPAAK
AAPAPAAAKPALPQVAANVDFAALGDRPEERVPMSRLRARIAERLVQSQSTNAILTTFNEVNMKPVMDLRNKYKDRFEKE
HGVKLGFMSFFVKAAVHALKKYPVINASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSLADIEKKIAEFGAKAR
DGKLSLEELTGGTFSISNGGTFGSMLSTPIINPPQSAILGVHATKDRAVVEDGQIVIRPMNYLAMSYDHRIIDGREAVLG
LVAMKEALEDPARLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=263, Percent_Identity=52.0912547528517, Blast_Score=281, Evalue=7e-76,
Organism=Homo sapiens, GI31711992, Length=443, Percent_Identity=32.5056433408578, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI110671329, Length=434, Percent_Identity=28.5714285714286, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI203098753, Length=454, Percent_Identity=30.1762114537445, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI203098816, Length=454, Percent_Identity=30.1762114537445, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=37.888198757764, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786946, Length=419, Percent_Identity=52.744630071599, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1786305, Length=431, Percent_Identity=32.0185614849188, Blast_Score=167, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=425, Percent_Identity=43.0588235294118, Blast_Score=317, Evalue=6e-87,
Organism=Caenorhabditis elegans, GI17560088, Length=452, Percent_Identity=32.0796460176991, Blast_Score=184, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17537937, Length=437, Percent_Identity=26.7734553775744, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=320, Percent_Identity=32.1875, Blast_Score=145, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6320352, Length=413, Percent_Identity=42.1307506053269, Blast_Score=322, Evalue=1e-88,
Organism=Saccharomyces cerevisiae, GI6324258, Length=455, Percent_Identity=29.010989010989, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24645909, Length=229, Percent_Identity=55.8951965065502, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI18859875, Length=440, Percent_Identity=28.6363636363636, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24582497, Length=230, Percent_Identity=33.0434782608696, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI20129315, Length=230, Percent_Identity=33.0434782608696, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43440; Mature: 43309

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVDVKVPQLSESVAEATMLNWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLSQI
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCEEEEEECCCCHHHHHHH
IKNDGDTVVADELIAKIDTEATAGAAAPAAAAPAPAAAAPAAAAAAAPAAAGAVAMPSAA
HHCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHCCCCCCCHHH
KLMAEAGLSAGQVAGTGKDGRITKGDVLGASAAPAPAPAAKAAPAPAAAKPALPQVAANV
HHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCC
DFAALGDRPEERVPMSRLRARIAERLVQSQSTNAILTTFNEVNMKPVMDLRNKYKDRFEK
CEEECCCCCHHCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHH
EHGVKLGFMSFFVKAAVHALKKYPVINASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRN
HHCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEEEEEEEEECCCCCEEEEEECC
ADQMSLADIEKKIAEFGAKARDGKLSLEELTGGTFSISNGGTFGSMLSTPIINPPQSAIL
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEECCCCCHHHHHCCCCCCCCHHHEE
GVHATKDRAVVEDGQIVIRPMNYLAMSYDHRIIDGREAVLGLVAMKEALEDPARLLLDL
EEECCCCCEEEECCCEEEEEHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
AIVDVKVPQLSESVAEATMLNWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLSQI
EEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCEEEEEECCCCHHHHHHH
IKNDGDTVVADELIAKIDTEATAGAAAPAAAAPAPAAAAPAAAAAAAPAAAGAVAMPSAA
HHCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHCCCCCCCHHH
KLMAEAGLSAGQVAGTGKDGRITKGDVLGASAAPAPAPAAKAAPAPAAAKPALPQVAANV
HHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCC
DFAALGDRPEERVPMSRLRARIAERLVQSQSTNAILTTFNEVNMKPVMDLRNKYKDRFEK
CEEECCCCCHHCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHH
EHGVKLGFMSFFVKAAVHALKKYPVINASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRN
HHCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEEEEEEEEECCCCCEEEEEECC
ADQMSLADIEKKIAEFGAKARDGKLSLEELTGGTFSISNGGTFGSMLSTPIINPPQSAIL
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEECCCCCHHHHHCCCCCCCCHHHEE
GVHATKDRAVVEDGQIVIRPMNYLAMSYDHRIIDGREAVLGLVAMKEALEDPARLLLDL
EEECCCCCEEEECCCEEEEEHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]