Click here to switch to the map view.

The map label for this gene is odhL [H]

Identifier: 73541733

GI number: 73541733

Start: 2244261

End: 2245685

Strand: Reverse

Name: odhL [H]

Synonym: Reut_A2045

Alternate gene names: 73541733

Gene position: 2245685-2244261 (Counterclockwise)

Preceding gene: 73541734

Following gene: 73541732

Centisome position: 59.0

GC content: 63.58

Gene sequence:

>1425_bases
ATGAGCAAACAATTCGACGTGCTGGTGATCGGCGCCGGCCCCGGCGGCTATATCGCCGCTATCCGTGCCGGCCAGCTCGG
CCTGAACGTGGCGTGCTGCGAAGGCAACGCCTACGACGATCCCAAGAACGAACCGCGCCTGGGCGGCACCTGCCTGAACG
TGGGTTGCATTCCCTCGAAGGCGCTGCTGGCTTCTTCGGAAGAATTCGAGAACGCCCAGCACCATCTGGCCGACCACGGC
ATTACCGTGGGCGACGTCAAGGTCGACGTGTCCAAGATGCTCAAGCGCAAGGACGACATCGTCGGCAAGATGACCAAGGG
CATCGAGTTCCTGTTCCGCAAGAACAAGGTGACGCTGCTCAAGGGCTACGGCAAGTTCGTCGGCAAGACCGCCGAGGGCT
TCCAGGTCGAGGTCGCCGGTGAAGTCGTGACCGCAAAGCAAGTCATCATCGCTACTGGTTCGAAGGCTCGCCATCTTCCG
GGTATCACGGTCGACAACGACCTGGTCAGCGATAACGAAGGCGCGCTCAAGTTCCCAGCAGTGCCGAAGAAGCTGGGCGT
GATCGGCGCCGGCGTGATCGGCCTGGAGCTGGGCTCGGTGTGGCGCCGTCTCGGTGCTGAAGTGACCGTGCTGGAAGCGC
TGCCCGCATTCCTGGGTGCCGCTGACGAAGGCGTGGCCAAGGAAGCGCAGAAGCAGCTGACCAAGCAGGGCCTGAAGTTC
AGCCTCGGCGTGAAGGTTGACGAAGTGACGACCGGCAAGAGCGGCGTGACCGTCAAGTACACCGACAAGGATGGCGCTGC
CCAGACGCTGGAAGTCGATCGCCTGATCGTGTCGGTCGGCCGCGTCCCGAACACCGACAACCTGGGCCTGGAAGCTGTCG
GCCTGGCCGCTGATCAGCGCGGCTTCATCGAAGTCGACGATCACTGCGCCACCAAGGTGCCGGGCCTGTGGGCCATCGGC
GACGTGGTGCGTGGCCCGATGCTCGCCCACAAGGCCGAGGACGAAGGCGTGGCTGTGGCCGAGCGCATCGCCGGCCAGAA
GCCGCACATCGACTACAACTGCATTCCGTGGGTGATCTACACCTTCCCGGAAATCGCATGGGTGGGCAAGACCGAAGCAC
AGCTCAAGGCCGAAGGCCGCGAGTTCAAGGCTGGCCAGTTCCCGTTCATGGCCAATGGCCGTGCACTCGGCATGGGTCAT
CCGGAAGGCTTCGTCAAGATGCTGGCCGATGCCAAGACCGACGAGATCCTGGGCGTGCACATCGTCGCCGCCAACGCGTC
GGACCTGATCGCCGAGGCCGTTGTGGCGATGGAGTTCAAGGCGGCGAGCGAAGATATCGGCCGCGTCTGCCATCCGCACC
CGTCGATGTCGGAAGTCATGCGCGAAGCCGCGCTGGCCGTCGACAAGCGTCAGCTCAATATGTAA

Upstream 100 bases:

>100_bases
GGTTTCCCCGCGCGCTGCCGCAATCGGTCGCGCGGGGCGTGTTCCCAGTGTTGAGGGAGTGCCGGCACAAGCTGCTGCCG
GCGCCCAGAAAGGAATTTCC

Downstream 100 bases:

>100_bases
GGCATTTGCCTGCATTTGCCTGAGCACCGGCGCGCCGCAACAGCGGCGCGCCGTTTTCACTTGCAAACCATGCCGGATCG
AAACGGTGCCGGGCATGGCC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSKQFDVLVIGAGPGGYIAAIRAGQLGLNVACCEGNAYDDPKNEPRLGGTCLNVGCIPSKALLASSEEFENAQHHLADHG
ITVGDVKVDVSKMLKRKDDIVGKMTKGIEFLFRKNKVTLLKGYGKFVGKTAEGFQVEVAGEVVTAKQVIIATGSKARHLP
GITVDNDLVSDNEGALKFPAVPKKLGVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEGVAKEAQKQLTKQGLKF
SLGVKVDEVTTGKSGVTVKYTDKDGAAQTLEVDRLIVSVGRVPNTDNLGLEAVGLAADQRGFIEVDDHCATKVPGLWAIG
DVVRGPMLAHKAEDEGVAVAERIAGQKPHIDYNCIPWVIYTFPEIAWVGKTEAQLKAEGREFKAGQFPFMANGRALGMGH
PEGFVKMLADAKTDEILGVHIVAANASDLIAEAVVAMEFKAASEDIGRVCHPHPSMSEVMREAALAVDKRQLNM

Sequences:

>Translated_474_residues
MSKQFDVLVIGAGPGGYIAAIRAGQLGLNVACCEGNAYDDPKNEPRLGGTCLNVGCIPSKALLASSEEFENAQHHLADHG
ITVGDVKVDVSKMLKRKDDIVGKMTKGIEFLFRKNKVTLLKGYGKFVGKTAEGFQVEVAGEVVTAKQVIIATGSKARHLP
GITVDNDLVSDNEGALKFPAVPKKLGVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEGVAKEAQKQLTKQGLKF
SLGVKVDEVTTGKSGVTVKYTDKDGAAQTLEVDRLIVSVGRVPNTDNLGLEAVGLAADQRGFIEVDDHCATKVPGLWAIG
DVVRGPMLAHKAEDEGVAVAERIAGQKPHIDYNCIPWVIYTFPEIAWVGKTEAQLKAEGREFKAGQFPFMANGRALGMGH
PEGFVKMLADAKTDEILGVHIVAANASDLIAEAVVAMEFKAASEDIGRVCHPHPSMSEVMREAALAVDKRQLNM
>Mature_473_residues
SKQFDVLVIGAGPGGYIAAIRAGQLGLNVACCEGNAYDDPKNEPRLGGTCLNVGCIPSKALLASSEEFENAQHHLADHGI
TVGDVKVDVSKMLKRKDDIVGKMTKGIEFLFRKNKVTLLKGYGKFVGKTAEGFQVEVAGEVVTAKQVIIATGSKARHLPG
ITVDNDLVSDNEGALKFPAVPKKLGVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEGVAKEAQKQLTKQGLKFS
LGVKVDEVTTGKSGVTVKYTDKDGAAQTLEVDRLIVSVGRVPNTDNLGLEAVGLAADQRGFIEVDDHCATKVPGLWAIGD
VVRGPMLAHKAEDEGVAVAERIAGQKPHIDYNCIPWVIYTFPEIAWVGKTEAQLKAEGREFKAGQFPFMANGRALGMGHP
EGFVKMLADAKTDEILGVHIVAANASDLIAEAVVAMEFKAASEDIGRVCHPHPSMSEVMREAALAVDKRQLNM

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=467, Percent_Identity=49.4646680942184, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI50301238, Length=472, Percent_Identity=28.6016949152542, Blast_Score=162, Evalue=9e-40,
Organism=Homo sapiens, GI33519430, Length=447, Percent_Identity=26.3982102908277, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI33519428, Length=447, Percent_Identity=26.3982102908277, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI33519426, Length=447, Percent_Identity=26.3982102908277, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI148277065, Length=447, Percent_Identity=26.3982102908277, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI148277071, Length=447, Percent_Identity=26.3982102908277, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI291045266, Length=441, Percent_Identity=25.1700680272109, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI22035672, Length=461, Percent_Identity=27.9826464208243, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI291045268, Length=435, Percent_Identity=24.1379310344828, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786307, Length=458, Percent_Identity=38.4279475982533, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI87082354, Length=471, Percent_Identity=29.5116772823779, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789915, Length=464, Percent_Identity=28.2327586206897, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87081717, Length=497, Percent_Identity=24.748490945674, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32565766, Length=464, Percent_Identity=52.1551724137931, Blast_Score=459, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17557007, Length=477, Percent_Identity=27.6729559748428, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI71982272, Length=499, Percent_Identity=26.6533066132265, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71983429, Length=466, Percent_Identity=28.755364806867, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71983419, Length=466, Percent_Identity=28.755364806867, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17559934, Length=193, Percent_Identity=32.1243523316062, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=477, Percent_Identity=46.7505241090147, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6325240, Length=482, Percent_Identity=31.9502074688797, Blast_Score=220, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6325166, Length=477, Percent_Identity=27.0440251572327, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI21358499, Length=473, Percent_Identity=51.1627906976744, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24640549, Length=493, Percent_Identity=27.5862068965517, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640553, Length=493, Percent_Identity=27.5862068965517, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640551, Length=493, Percent_Identity=27.5862068965517, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17737741, Length=491, Percent_Identity=25.2545824847251, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50308; Mature: 50177

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQFDVLVIGAGPGGYIAAIRAGQLGLNVACCEGNAYDDPKNEPRLGGTCLNVGCIPSK
CCCCEEEEEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCH
ALLASSEEFENAQHHLADHGITVGDVKVDVSKMLKRKDDIVGKMTKGIEFLFRKNKVTLL
HHHCCCHHHHHHHHHHHHCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
KGYGKFVGKTAEGFQVEVAGEVVTAKQVIIATGSKARHLPGITVDNDLVSDNEGALKFPA
ECCHHHHCCCCCCEEEEEECCEEEEEEEEEECCCCCCCCCCCEECCCCCCCCCCCEECCC
VPKKLGVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEGVAKEAQKQLTKQGLKF
CCHHHCEEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEE
SLGVKVDEVTTGKSGVTVKYTDKDGAAQTLEVDRLIVSVGRVPNTDNLGLEAVGLAADQR
EECEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
GFIEVDDHCATKVPGLWAIGDVVRGPMLAHKAEDEGVAVAERIAGQKPHIDYNCIPWVIY
CCEEECCCHHHCCCCCEEHHHHHCCCHHEECCCCCCHHHHHHHCCCCCCCCCCCCCEEEE
TFPEIAWVGKTEAQLKAEGREFKAGQFPFMANGRALGMGHPEGFVKMLADAKTDEILGVH
ECCCEEECCCCHHHHHCCCCCCCCCCCCEEECCEEEECCCCHHHHHHHHCCCCCCEEEEE
IVAANASDLIAEAVVAMEFKAASEDIGRVCHPHPSMSEVMREAALAVDKRQLNM
EEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKQFDVLVIGAGPGGYIAAIRAGQLGLNVACCEGNAYDDPKNEPRLGGTCLNVGCIPSK
CCCEEEEEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCH
ALLASSEEFENAQHHLADHGITVGDVKVDVSKMLKRKDDIVGKMTKGIEFLFRKNKVTLL
HHHCCCHHHHHHHHHHHHCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
KGYGKFVGKTAEGFQVEVAGEVVTAKQVIIATGSKARHLPGITVDNDLVSDNEGALKFPA
ECCHHHHCCCCCCEEEEEECCEEEEEEEEEECCCCCCCCCCCEECCCCCCCCCCCEECCC
VPKKLGVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEGVAKEAQKQLTKQGLKF
CCHHHCEEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEE
SLGVKVDEVTTGKSGVTVKYTDKDGAAQTLEVDRLIVSVGRVPNTDNLGLEAVGLAADQR
EECEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
GFIEVDDHCATKVPGLWAIGDVVRGPMLAHKAEDEGVAVAERIAGQKPHIDYNCIPWVIY
CCEEECCCHHHCCCCCEEHHHHHCCCHHEECCCCCCHHHHHHHCCCCCCCCCCCCCEEEE
TFPEIAWVGKTEAQLKAEGREFKAGQFPFMANGRALGMGHPEGFVKMLADAKTDEILGVH
ECCCEEECCCCHHHHHCCCCCCCCCCCCEEECCEEEECCCCHHHHHHHHCCCCCCEEEEE
IVAANASDLIAEAVVAMEFKAASEDIGRVCHPHPSMSEVMREAALAVDKRQLNM
EEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]