Click here to switch to the map view.

The map label for this gene is clpB [H]

Identifier: 73541668

GI number: 73541668

Start: 2168504

End: 2171092

Strand: Reverse

Name: clpB [H]

Synonym: Reut_A1980

Alternate gene names: 73541668

Gene position: 2171092-2168504 (Counterclockwise)

Preceding gene: 73541669

Following gene: 73541666

Centisome position: 57.04

GC content: 63.38

Gene sequence:

>2589_bases
ATGCGTCTTGACAAACTGACTACCCGGTTCCAGGAAGCGCTGGCTGATGCGCAAAGCCTGGCACTGGCCAATGACAACCA
ATACATCGAACCGCAGCACCTGCTGCGTGCGCTGATTGCGCAGAACGACGGCGCAGCCAAGGCGCTGCTGCAGCGCGCCG
GCGTCAACGTGAACGGCTTGCAGACCGCGCTGGATGCGGCCATCAAGCGTCTGCCGCAAGTGCAGGGCACCAGCGAGGTC
CAGGTGGGCCGCGATCTGGTCAATCTGCTTAACGCCGCCGAGAAAGAATCCATCAAGCGCGGCGACCAGTTCATCGCCAG
CGAGCTGTTCCTGCTTGCCGTGTCGGAAGACAAAGGCGAGGCCGGGCGCATTGCGCGCGAGAACGGGCTGAACCGCAAGC
CATTGGAAGCGGCCATCCAGGCCGTGCGCGGCGGCGATGCCGTCAATAGCGCCGACGCCGAAACGCAGCGTGAGGCGCTC
AAGAAGTACACCATCGACCTGACCGAGCGCGCTCGAATGGGCAAGCTCGACCCGGTGATCGGCCGCGACGACGAGATTCG
ACGTGCGATTCAGATCCTGCAGCGCCGCACCAAGAACAACCCGGTCCTGATCGGCGAGCCGGGCGTGGGCAAGACCGCGA
TCGTCGAAGGCCTCGCGCAGCGCATCGTCAACGGCGAAGTGCCCGAAAGCCTGAAGAACAAGCGCGTGCTGGTGCTCGAC
ATGGCCGGCCTGCTGGCTGGCGCCAAGTACCGCGGCGAGTTCGAGGAACGCCTGAAGGCCGTGCTGAACGATGTGGCCAA
GGATGAAGGCCAGACCATCGTCTTTATCGACGAGATCCACACCATGGTCGGCGCCGGCAAGGCTGAAGGCGCGATCGACG
CGGGCAACATGCTCAAGCCGGCGCTGGCGCGCGGCGAGCTGCACTGCATCGGCGCGACCACGCTGGACGAGTACCGCAAG
TACATCGAAAAGGACGCCGCGCTCGAGCGCCGTTTCCAGAAAGTGCTGGTCGATGAGCCGAGCGTGGAGGCGACCATCGC
CATCCTGCGCGGCTTGCAGGAAAAGTATGAGCTGCACCATGGCGTTGAAATCACCGACCCGGCCATCGTCGCTGCGGCGG
AGCTGTCGCATCGCTATATCACCGACCGCTTCCTGCCCGACAAGGCCATCGACCTGATCGACGAGGCGGCTGCGCGCATC
AAGATGGAAATCGACTCCAAGCCGGAGGCGATGGACAAGCTCGAGCGCCGCACCATCCAGCTCAAGATCGAACGCGAGGC
GGTCAAGAAAGAGACCGACGAAGCGTCGCTCAAGCGCCTGGACCTGATCGAGCAGGAGATCACGCGCCTGGAAAAGGAGT
ATGCGGATCTCGACGAGATCTGGAAGGCCGAAAAGGGCGCCGCTCAGGGTACGGCTGCGCTGAAGGAAGAGATCGAGAAG
GTCAAGCTGGAGATCGCCAAGCTGCAGCGCGAAGGCAAGCTCGACAAGGTGGCGGAACTGCAGTACGGCAAGCTGCCGGG
CCTGGAGGGCAAACTCAAGGCTGCGACCGACGCCGAAGCGAGCGAGCAGAAGCAGCCCAACAAGCTGTTGCGCACGCAAG
TCGGTGCCGAGGAAATCGCCGAAGTGGTGAGCCGCGCGACGGGCATCCCGGTATCGAAGATGATGCAGGGCGAGCGCGAG
AAGCTGCTCAAGATGGAAGACCGACTGCACAACCGCGTGGTGGGCCAGGACGAAGCCGTGCGGCTGGTGTCCGATGCCAT
CCGCCGTTCGCGTGCGGGCCTCGCGGACGAGAACAAGCCGTATGGCTCGTTCCTGTTCCTGGGACCGACCGGTGTCGGCA
AGACCGAACTGTGCAAGGCCCTGGCCGAGTTCCTGTTCGATTCGGAAGAGCACCTGATCCGCATCGACATGAGCGAGTTC
ATGGAGAAGCACAGCGTGAGCCGCCTGATCGGCGCGCCGCCGGGATATGTCGGATATGAAGAGGGTGGTTACCTGACCGA
AGCCGTGCGCCGCAAGCCGTACAGCGTGGTGCTGCTCGACGAAGTCGAGAAGGCACACCCGGACGTTTTCAACGTGCTGC
TGCAGGTGCTGGACGATGGCCGCCTGACCGATGGCCAGGGACGTACGGTGGACTTCAAGAACACCGTGATCGTGATGACG
TCCAACCTCGGTTCGCAGATCATCCAGTCGATGGTGGGCGAGCCGCAGGAAGCGGTGAAGGGCGCGGTATGGCAGGAGGT
GCGCACGCATTTCCGGCCCGAGTTCCTGAACCGGATCGACGAAGTCGTGGTGTTCCACGCACTCGACCAGAAGAACATCG
AGTCGATCGCGCGCATCCAGCTGCAACGCCTGCAGGGTCGCCTGGCCCGCATGGACATGACGCTGGAGGTCAGCGAACTG
GCGCTGGAGAAGATCGCGAGCGCGGGTTACGACCCGGTGTTCGGGGCCCGTCCGCTCAAGCGGGCGATCCAGCAGCAGAT
CGAAAACCCGGTGGCACGTGCCATCCTGGAAGGAAAGTTTGCTGCGAAGGATGTGGTGCCGGTGGACTATGCCGATGGGG
CGTTTGTGATCGGTGGAGTTGCCCAGTAA

Upstream 100 bases:

>100_bases
CTTGAAATGTCAAGTCAAAGCCTTATCTTTCGGAAAGACATCCAGTGTCCGGCGGGGCGAGCTTCCTAGCATCAGGACAC
TCACTGAGAGAGGGAATCCT

Downstream 100 bases:

>100_bases
GCGCGAAGCCTGACAGCAATGTCGCCTTCATGTGCTTGCCAAAAACCCGCCGCAGTGATGCGGCGGGTTTTTTGTTGTCC
CGGGCTTACCAGTTGAACCG

Product: AAA ATPase, central region:Clp, N terminal

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 862; Mature: 862

Protein sequence:

>862_residues
MRLDKLTTRFQEALADAQSLALANDNQYIEPQHLLRALIAQNDGAAKALLQRAGVNVNGLQTALDAAIKRLPQVQGTSEV
QVGRDLVNLLNAAEKESIKRGDQFIASELFLLAVSEDKGEAGRIARENGLNRKPLEAAIQAVRGGDAVNSADAETQREAL
KKYTIDLTERARMGKLDPVIGRDDEIRRAIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPESLKNKRVLVLD
MAGLLAGAKYRGEFEERLKAVLNDVAKDEGQTIVFIDEIHTMVGAGKAEGAIDAGNMLKPALARGELHCIGATTLDEYRK
YIEKDAALERRFQKVLVDEPSVEATIAILRGLQEKYELHHGVEITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAARI
KMEIDSKPEAMDKLERRTIQLKIEREAVKKETDEASLKRLDLIEQEITRLEKEYADLDEIWKAEKGAAQGTAALKEEIEK
VKLEIAKLQREGKLDKVAELQYGKLPGLEGKLKAATDAEASEQKQPNKLLRTQVGAEEIAEVVSRATGIPVSKMMQGERE
KLLKMEDRLHNRVVGQDEAVRLVSDAIRRSRAGLADENKPYGSFLFLGPTGVGKTELCKALAEFLFDSEEHLIRIDMSEF
MEKHSVSRLIGAPPGYVGYEEGGYLTEAVRRKPYSVVLLDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTVIVMT
SNLGSQIIQSMVGEPQEAVKGAVWQEVRTHFRPEFLNRIDEVVVFHALDQKNIESIARIQLQRLQGRLARMDMTLEVSEL
ALEKIASAGYDPVFGARPLKRAIQQQIENPVARAILEGKFAAKDVVPVDYADGAFVIGGVAQ

Sequences:

>Translated_862_residues
MRLDKLTTRFQEALADAQSLALANDNQYIEPQHLLRALIAQNDGAAKALLQRAGVNVNGLQTALDAAIKRLPQVQGTSEV
QVGRDLVNLLNAAEKESIKRGDQFIASELFLLAVSEDKGEAGRIARENGLNRKPLEAAIQAVRGGDAVNSADAETQREAL
KKYTIDLTERARMGKLDPVIGRDDEIRRAIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPESLKNKRVLVLD
MAGLLAGAKYRGEFEERLKAVLNDVAKDEGQTIVFIDEIHTMVGAGKAEGAIDAGNMLKPALARGELHCIGATTLDEYRK
YIEKDAALERRFQKVLVDEPSVEATIAILRGLQEKYELHHGVEITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAARI
KMEIDSKPEAMDKLERRTIQLKIEREAVKKETDEASLKRLDLIEQEITRLEKEYADLDEIWKAEKGAAQGTAALKEEIEK
VKLEIAKLQREGKLDKVAELQYGKLPGLEGKLKAATDAEASEQKQPNKLLRTQVGAEEIAEVVSRATGIPVSKMMQGERE
KLLKMEDRLHNRVVGQDEAVRLVSDAIRRSRAGLADENKPYGSFLFLGPTGVGKTELCKALAEFLFDSEEHLIRIDMSEF
MEKHSVSRLIGAPPGYVGYEEGGYLTEAVRRKPYSVVLLDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTVIVMT
SNLGSQIIQSMVGEPQEAVKGAVWQEVRTHFRPEFLNRIDEVVVFHALDQKNIESIARIQLQRLQGRLARMDMTLEVSEL
ALEKIASAGYDPVFGARPLKRAIQQQIENPVARAILEGKFAAKDVVPVDYADGAFVIGGVAQ
>Mature_862_residues
MRLDKLTTRFQEALADAQSLALANDNQYIEPQHLLRALIAQNDGAAKALLQRAGVNVNGLQTALDAAIKRLPQVQGTSEV
QVGRDLVNLLNAAEKESIKRGDQFIASELFLLAVSEDKGEAGRIARENGLNRKPLEAAIQAVRGGDAVNSADAETQREAL
KKYTIDLTERARMGKLDPVIGRDDEIRRAIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPESLKNKRVLVLD
MAGLLAGAKYRGEFEERLKAVLNDVAKDEGQTIVFIDEIHTMVGAGKAEGAIDAGNMLKPALARGELHCIGATTLDEYRK
YIEKDAALERRFQKVLVDEPSVEATIAILRGLQEKYELHHGVEITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAARI
KMEIDSKPEAMDKLERRTIQLKIEREAVKKETDEASLKRLDLIEQEITRLEKEYADLDEIWKAEKGAAQGTAALKEEIEK
VKLEIAKLQREGKLDKVAELQYGKLPGLEGKLKAATDAEASEQKQPNKLLRTQVGAEEIAEVVSRATGIPVSKMMQGERE
KLLKMEDRLHNRVVGQDEAVRLVSDAIRRSRAGLADENKPYGSFLFLGPTGVGKTELCKALAEFLFDSEEHLIRIDMSEF
MEKHSVSRLIGAPPGYVGYEEGGYLTEAVRRKPYSVVLLDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTVIVMT
SNLGSQIIQSMVGEPQEAVKGAVWQEVRTHFRPEFLNRIDEVVVFHALDQKNIESIARIQLQRLQGRLARMDMTLEVSEL
ALEKIASAGYDPVFGARPLKRAIQQQIENPVARAILEGKFAAKDVVPVDYADGAFVIGGVAQ

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=307, Percent_Identity=37.785016286645, Blast_Score=202, Evalue=1e-51,
Organism=Escherichia coli, GI1788943, Length=848, Percent_Identity=67.4528301886792, Blast_Score=1153, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=434, Percent_Identity=39.4009216589862, Blast_Score=287, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6320464, Length=711, Percent_Identity=53.1645569620253, Blast_Score=753, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=859, Percent_Identity=43.3061699650757, Blast_Score=665, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95327; Mature: 95327

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKLTTRFQEALADAQSLALANDNQYIEPQHLLRALIAQNDGAAKALLQRAGVNVNGL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCHHH
QTALDAAIKRLPQVQGTSEVQVGRDLVNLLNAAEKESIKRGDQFIASELFLLAVSEDKGE
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCC
AGRIARENGLNRKPLEAAIQAVRGGDAVNSADAETQREALKKYTIDLTERARMGKLDPVI
CCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GRDDEIRRAIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPESLKNKRVLVLD
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHCCCEEEEEE
MAGLLAGAKYRGEFEERLKAVLNDVAKDEGQTIVFIDEIHTMVGAGKAEGAIDAGNMLKP
HHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHHH
ALARGELHCIGATTLDEYRKYIEKDAALERRFQKVLVDEPSVEATIAILRGLQEKYELHH
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
GVEITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAARIKMEIDSKPEAMDKLERRTIQ
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHEE
LKIEREAVKKETDEASLKRLDLIEQEITRLEKEYADLDEIWKAEKGAAQGTAALKEEIEK
EEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHH
VKLEIAKLQREGKLDKVAELQYGKLPGLEGKLKAATDAEASEQKQPNKLLRTQVGAEEIA
HHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCCHHHHH
EVVSRATGIPVSKMMQGEREKLLKMEDRLHNRVVGQDEAVRLVSDAIRRSRAGLADENKP
HHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
YGSFLFLGPTGVGKTELCKALAEFLFDSEEHLIRIDMSEFMEKHSVSRLIGAPPGYVGYE
CCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCC
EGGYLTEAVRRKPYSVVLLDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTVIVMT
CCCHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEE
SNLGSQIIQSMVGEPQEAVKGAVWQEVRTHFRPEFLNRIDEVVVFHALDQKNIESIARIQ
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHH
LQRLQGRLARMDMTLEVSELALEKIASAGYDPVFGARPLKRAIQQQIENPVARAILEGKF
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
AAKDVVPVDYADGAFVIGGVAQ
HCCCCCCCCCCCCCEEECCCCC
>Mature Secondary Structure
MRLDKLTTRFQEALADAQSLALANDNQYIEPQHLLRALIAQNDGAAKALLQRAGVNVNGL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCHHH
QTALDAAIKRLPQVQGTSEVQVGRDLVNLLNAAEKESIKRGDQFIASELFLLAVSEDKGE
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCC
AGRIARENGLNRKPLEAAIQAVRGGDAVNSADAETQREALKKYTIDLTERARMGKLDPVI
CCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GRDDEIRRAIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPESLKNKRVLVLD
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHCCCEEEEEE
MAGLLAGAKYRGEFEERLKAVLNDVAKDEGQTIVFIDEIHTMVGAGKAEGAIDAGNMLKP
HHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHHH
ALARGELHCIGATTLDEYRKYIEKDAALERRFQKVLVDEPSVEATIAILRGLQEKYELHH
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
GVEITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAARIKMEIDSKPEAMDKLERRTIQ
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHEE
LKIEREAVKKETDEASLKRLDLIEQEITRLEKEYADLDEIWKAEKGAAQGTAALKEEIEK
EEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHH
VKLEIAKLQREGKLDKVAELQYGKLPGLEGKLKAATDAEASEQKQPNKLLRTQVGAEEIA
HHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCCHHHHH
EVVSRATGIPVSKMMQGEREKLLKMEDRLHNRVVGQDEAVRLVSDAIRRSRAGLADENKP
HHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
YGSFLFLGPTGVGKTELCKALAEFLFDSEEHLIRIDMSEFMEKHSVSRLIGAPPGYVGYE
CCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCC
EGGYLTEAVRRKPYSVVLLDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTVIVMT
CCCHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEE
SNLGSQIIQSMVGEPQEAVKGAVWQEVRTHFRPEFLNRIDEVVVFHALDQKNIESIARIQ
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHH
LQRLQGRLARMDMTLEVSELALEKIASAGYDPVFGARPLKRAIQQQIENPVARAILEGKF
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
AAKDVVPVDYADGAFVIGGVAQ
HCCCCCCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]