Click here to switch to the map view.

The map label for this gene is sbp [H]

Identifier: 73541666

GI number: 73541666

Start: 2163984

End: 2164991

Strand: Reverse

Name: sbp [H]

Synonym: Reut_A1978

Alternate gene names: 73541666

Gene position: 2164991-2163984 (Counterclockwise)

Preceding gene: 73541668

Following gene: 73541665

Centisome position: 56.88

GC content: 62.7

Gene sequence:

>1008_bases
ATGATCCGCAAGCTGGCCATTGGCCTGGCCGCATTTGCCGCTATCGGCGCCGCGCAGACGGCGACGGCAACCAATCTGCT
GAACGTTTCGTATGATCCGACGCGCGAACTGTACGTGGACGTGAACGCCGCGTTTGCCAAGGCCTGGAAGGCGCAGGGCG
GCGATACCGTGACAGTACGCCAGTCGCACGGCGGCTCGGGCAAGCAGGCCCGCTCCGTGATTGACGGCCTTGATGCCGAT
GTGGTTACGCTGGCGCTCGGCTACGACATCGACGCGATTGCCGAGAAAGGCCTGATCAAGCCCGACTGGCAGAAGCGCTT
GCCGCACAACGCCTCGCCGTACACCTCGACCATCGTGTTCCTGGTGCGCAAAGGTAACCCGAAAGGCATCAAGGACTGGA
ACGACCTGGTGAAGCCAGGCGTGCAGGTCATCACGCCCAATCCCAAGACTTCGGGCGGCGCGCGCTGGAACTACCTGGCC
GCATGGGGCTATGCGCAGCGACAGCCGGGCGGCAATGACGCCAAGGCGCGCGAATTTGTTGGCGAACTGCTCAAGCACGT
GCCCGTGCTCGATTCCGGCGCGCGCGGCGCGACCACGACCTTTGTCGAGCGCGGCCTTGGCGACGTGCTGATCGCATGGG
AAAACGAAGCGATCCTGGCGATCAAGGAACTCGGTCCGGACAAGTTTGACATCGTCGCGCCGTCGGTCTCGATCCTGGCC
GAGCCGCCCGTCGCGGTGGTCGACAAGGTGGTCGACAAGAAGGGCACGCGCAAGGTGGCCGAAGCCTACCTGCAGTTCCT
GTACACCGATGAAGGCCAGGAAATCGCTGCCAAGAACTACTACCGTCCGATTTCAGCGAAGGTGGCCGCCAAGTACGCGG
CAAACTTCCCGAAGGTGAAGCTGTTCACCATCGATGAAGCTTTCGGCGGCTGGCAGAAGGCGCAGAAGACCCACTTTGCC
GATGGCGGTTCGTTCGACCAGATCTACCAGCCCGGCAAGAAGCAATAG

Upstream 100 bases:

>100_bases
CCTCGGTAGTCTTGCTCCTGACATAAGCCCGACCACACATTGCGGGCAAGCCAATGAGGCGCCTCCGGCGCCAGTCACGA
GACCTGACGGGAGCAACAAG

Downstream 100 bases:

>100_bases
TCATCCAGAAGACGGACGGGCCGGCCCCAAGGTTTCGGGCGACCGGCACCGTCCCGCCAACCAGAAGAAGGAAGCCCCGA
TGTCGATCCTTACGCTGTCC

Product: thiosulphate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MIRKLAIGLAAFAAIGAAQTATATNLLNVSYDPTRELYVDVNAAFAKAWKAQGGDTVTVRQSHGGSGKQARSVIDGLDAD
VVTLALGYDIDAIAEKGLIKPDWQKRLPHNASPYTSTIVFLVRKGNPKGIKDWNDLVKPGVQVITPNPKTSGGARWNYLA
AWGYAQRQPGGNDAKAREFVGELLKHVPVLDSGARGATTTFVERGLGDVLIAWENEAILAIKELGPDKFDIVAPSVSILA
EPPVAVVDKVVDKKGTRKVAEAYLQFLYTDEGQEIAAKNYYRPISAKVAAKYAANFPKVKLFTIDEAFGGWQKAQKTHFA
DGGSFDQIYQPGKKQ

Sequences:

>Translated_335_residues
MIRKLAIGLAAFAAIGAAQTATATNLLNVSYDPTRELYVDVNAAFAKAWKAQGGDTVTVRQSHGGSGKQARSVIDGLDAD
VVTLALGYDIDAIAEKGLIKPDWQKRLPHNASPYTSTIVFLVRKGNPKGIKDWNDLVKPGVQVITPNPKTSGGARWNYLA
AWGYAQRQPGGNDAKAREFVGELLKHVPVLDSGARGATTTFVERGLGDVLIAWENEAILAIKELGPDKFDIVAPSVSILA
EPPVAVVDKVVDKKGTRKVAEAYLQFLYTDEGQEIAAKNYYRPISAKVAAKYAANFPKVKLFTIDEAFGGWQKAQKTHFA
DGGSFDQIYQPGKKQ
>Mature_335_residues
MIRKLAIGLAAFAAIGAAQTATATNLLNVSYDPTRELYVDVNAAFAKAWKAQGGDTVTVRQSHGGSGKQARSVIDGLDAD
VVTLALGYDIDAIAEKGLIKPDWQKRLPHNASPYTSTIVFLVRKGNPKGIKDWNDLVKPGVQVITPNPKTSGGARWNYLA
AWGYAQRQPGGNDAKAREFVGELLKHVPVLDSGARGATTTFVERGLGDVLIAWENEAILAIKELGPDKFDIVAPSVSILA
EPPVAVVDKVVDKKGTRKVAEAYLQFLYTDEGQEIAAKNYYRPISAKVAAKYAANFPKVKLFTIDEAFGGWQKAQKTHFA
DGGSFDQIYQPGKKQ

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=327, Percent_Identity=70.0305810397553, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1788765, Length=313, Percent_Identity=50.4792332268371, Blast_Score=314, Evalue=5e-87,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36170; Mature: 36170

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS00401 PROK_SULFATE_BIND_1 ; PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKLAIGLAAFAAIGAAQTATATNLLNVSYDPTRELYVDVNAAFAKAWKAQGGDTVTVR
CCHHHHHHHHHHHHHCCHHHHHHHHEEECCCCCCCEEEEECHHHHHHHHHCCCCCEEEEE
QSHGGSGKQARSVIDGLDADVVTLALGYDIDAIAEKGLIKPDWQKRLPHNASPYTSTIVF
CCCCCCCHHHHHHHCCCCCCCEEEEECCCHHHHHHCCCCCCCHHHHCCCCCCCCCEEEEE
LVRKGNPKGIKDWNDLVKPGVQVITPNPKTSGGARWNYLAAWGYAQRQPGGNDAKAREFV
EEECCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCEEEEEECCCHHCCCCCCCHHHHHHH
GELLKHVPVLDSGARGATTTFVERGLGDVLIAWENEAILAIKELGPDKFDIVAPSVSILA
HHHHHHCCCCCCCCCCCHHHHHHCCCCCEEEEECCCEEEEEECCCCCCEEEECCCHHEEC
EPPVAVVDKVVDKKGTRKVAEAYLQFLYTDEGQEIAAKNYYRPISAKVAAKYAANFPKVK
CCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCEE
LFTIDEAFGGWQKAQKTHFADGGSFDQIYQPGKKQ
EEEECCHHCCHHHHHHHCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MIRKLAIGLAAFAAIGAAQTATATNLLNVSYDPTRELYVDVNAAFAKAWKAQGGDTVTVR
CCHHHHHHHHHHHHHCCHHHHHHHHEEECCCCCCCEEEEECHHHHHHHHHCCCCCEEEEE
QSHGGSGKQARSVIDGLDADVVTLALGYDIDAIAEKGLIKPDWQKRLPHNASPYTSTIVF
CCCCCCCHHHHHHHCCCCCCCEEEEECCCHHHHHHCCCCCCCHHHHCCCCCCCCCEEEEE
LVRKGNPKGIKDWNDLVKPGVQVITPNPKTSGGARWNYLAAWGYAQRQPGGNDAKAREFV
EEECCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCEEEEEECCCHHCCCCCCCHHHHHHH
GELLKHVPVLDSGARGATTTFVERGLGDVLIAWENEAILAIKELGPDKFDIVAPSVSILA
HHHHHHCCCCCCCCCCCHHHHHHCCCCCEEEEECCCEEEEEECCCCCCEEEECCCHHEEC
EPPVAVVDKVVDKKGTRKVAEAYLQFLYTDEGQEIAAKNYYRPISAKVAAKYAANFPKVK
CCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCEE
LFTIDEAFGGWQKAQKTHFADGGSFDQIYQPGKKQ
EEEECCHHCCHHHHHHHCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]