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The map label for this gene is def [H]

Identifier: 73541574

GI number: 73541574

Start: 2064675

End: 2065208

Strand: Reverse

Name: def [H]

Synonym: Reut_A1885

Alternate gene names: 73541574

Gene position: 2065208-2064675 (Counterclockwise)

Preceding gene: 73541575

Following gene: 73541570

Centisome position: 54.25

GC content: 64.23

Gene sequence:

>534_bases
ATGATCCGCGAGATTCTCAAGATGGGCGACGCGCGCCTGCTGCAGCAGGCGCGGCCGGTGGAGCGCTTCAATACGCCTGA
ACTGCGCACGCTGATCGAAGACATGTTCGACACCATGGACCACGCCAACGGCGCGGGGCTGGCGGCGCCGCAGATCGGTG
TGGACCTGCAGGTGGTGATCTTCGGCTTCGACCGGAACCCGCGCTATCCCGATGCGCCGAAGGTGCCGAAGACGGTGCTG
ATCAACCCGATGCTCGAAATGCTGTCCGACGATCTGGAGGATGGTTGGGAAGGCTGCCTGTCGGTGCCCGGCCTGCGCGG
CGTGGTGCCGCGCCATACGCGCCTGAGATACAGCGGCTACGACCTGATGGGCGGCAGCATCGACCGCGTCGCCGAAGGCT
TCCATGCGCGCGTGGTGCAGCATGAGTGCGACCACCTGCAAGGCATCCTGTATCCCATGCGGGTGCGCGACTTTACGCGC
TTCGGTTTTACCGAGGTATTGTTTCCGGAATTGCCGCCGAACAGCGACGACTGA

Upstream 100 bases:

>100_bases
AGCTCGACAAGGCCGAGGCGCTGGGTGTACCGGTGCTCGACGAGGCAGGAATGCTGGCGCTGCTGGAATCGGTCGGCGCG
GGCCAACCGGGGGAGCAGTC

Downstream 100 bases:

>100_bases
AGCGCGGCATGGACACAAAAGAAACGGGGCGCCAACTGGCGCCCCGTTTCTTTTGCGACCTCGCCGGCGACGTGCGCCGG
CGATGCGATGCCGATTCTCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MIREILKMGDARLLQQARPVERFNTPELRTLIEDMFDTMDHANGAGLAAPQIGVDLQVVIFGFDRNPRYPDAPKVPKTVL
INPMLEMLSDDLEDGWEGCLSVPGLRGVVPRHTRLRYSGYDLMGGSIDRVAEGFHARVVQHECDHLQGILYPMRVRDFTR
FGFTEVLFPELPPNSDD

Sequences:

>Translated_177_residues
MIREILKMGDARLLQQARPVERFNTPELRTLIEDMFDTMDHANGAGLAAPQIGVDLQVVIFGFDRNPRYPDAPKVPKTVL
INPMLEMLSDDLEDGWEGCLSVPGLRGVVPRHTRLRYSGYDLMGGSIDRVAEGFHARVVQHECDHLQGILYPMRVRDFTR
FGFTEVLFPELPPNSDD
>Mature_177_residues
MIREILKMGDARLLQQARPVERFNTPELRTLIEDMFDTMDHANGAGLAAPQIGVDLQVVIFGFDRNPRYPDAPKVPKTVL
INPMLEMLSDDLEDGWEGCLSVPGLRGVVPRHTRLRYSGYDLMGGSIDRVAEGFHARVVQHECDHLQGILYPMRVRDFTR
FGFTEVLFPELPPNSDD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=175, Percent_Identity=34.8571428571429, Blast_Score=87, Evalue=7e-18,
Organism=Escherichia coli, GI1789682, Length=149, Percent_Identity=36.241610738255, Blast_Score=90, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24645728, Length=171, Percent_Identity=32.1637426900585, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24645726, Length=156, Percent_Identity=34.6153846153846, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20049; Mature: 20049

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIREILKMGDARLLQQARPVERFNTPELRTLIEDMFDTMDHANGAGLAAPQIGVDLQVVI
CHHHHHHHCHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
FGFDRNPRYPDAPKVPKTVLINPMLEMLSDDLEDGWEGCLSVPGLRGVVPRHTRLRYSGY
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCC
DLMGGSIDRVAEGFHARVVQHECDHLQGILYPMRVRDFTRFGFTEVLFPELPPNSDD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIREILKMGDARLLQQARPVERFNTPELRTLIEDMFDTMDHANGAGLAAPQIGVDLQVVI
CHHHHHHHCHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
FGFDRNPRYPDAPKVPKTVLINPMLEMLSDDLEDGWEGCLSVPGLRGVVPRHTRLRYSGY
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCC
DLMGGSIDRVAEGFHARVVQHECDHLQGILYPMRVRDFTRFGFTEVLFPELPPNSDD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]