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The map label for this gene is ligA

Identifier: 73541575

GI number: 73541575

Start: 2065205

End: 2067340

Strand: Reverse

Name: ligA

Synonym: Reut_A1886

Alternate gene names: 73541575

Gene position: 2067340-2065205 (Counterclockwise)

Preceding gene: 73541576

Following gene: 73541574

Centisome position: 54.31

GC content: 66.39

Gene sequence:

>2136_bases
ATGAACGCAACCCATCGCGGTGCGCAGGCCGATGCCTCCGCGCCGGCTGGCGGCTTGCCGCCCGCAACTGCCGCGGAACG
CGTGCAATGGCTGCGCGACGAGCTGGACCGCCACAACTACCAGTATTACGTGCTCGACGCACCGACCATTCCGGATGCCG
AGTACGACGCGCTATTCACCGAACTGCAGGCACTCGAAGCCGAGCATCCCGAACTGCTGACGCCCGATTCGCCGACGCAA
CGCGTGGGCGGCGCGCCGCTGTCGGCCTTTGATACGGTACGCCACCGCGTGCCGATGCTGTCGCTGAACAACGGTTTCTC
CGACGAGGACGTCACCGGTTTTGACCGACGCTGCGCGCAAGGACTCGGGCGCAGCGCCGCAGCAGGCGGCGAGCAGGACT
TGTTCAGCGCCGCGGAAGGGGTGGAATACGCATGCGAGCTGAAATTCGACGGCCTGGCCATGTCGCTGCGCTACGAAGAC
GGTCGCCTGGTGCAGGCGGCCACGCGCGGCGACGGTGAAACCGGTGAAGACGTGACCGTCAATGTGCGCACCATCAAGGC
GATTCCGCTGAAGCTGCGCGGCGCGGCGCCTGCGGTGCTCGAGGTGCGCGGCGAAGTCTTCATGTACCGCCGGGATTTCG
ACAAGCTCAATGCGCGCCAGGCCGAGGCCGGCGATAAGACGTTCGTCAATCCGCGCAATGCCGCGGCCGGCAGCCTGCGG
CAGCTGGATCCGCGCATTACCGCGAAGCGTCCGCTTTCGTTCTTCGCATATGGCCTCGGTGAGTTGCAGGGCATCGAACG
TCCGGCTACTCACAGCGCGATGCTCGATGGCTTTGCGACCATGGGCCTGCCCGTCTGCAACGAGCGCGCGGTGGTGAAAG
GCGCACAAGGCCTGCTCGATTTCTACCGCAAGGTGGGCGAGAAGCGCGACCAGTTGCCGTATGACATCGATGGCGTGGTC
TACAAGGTCAACGCGCTGGCCGAGCAGGAACAGCTTGGCTTCGTGTCGCGCGCGCCGCGCTTCGCGCTCGCGCACAAGTT
CCCGGCGCAGGAGATGACCACCATCGTCGAGGACATCGAAGTGCAGGTCGGCCGCACCGGTGCCATTACGCCTGTCGCGC
GCCTGCAGCCGGTGTTCGTCGGTGGTGTGACGGTGACCAATGCCACCCTGCACAACGAGGACGAGGTGCGCCGCAAGGAC
GTGCATATCGGCGACACCGTGATCGTACGCCGCGCCGGCGATGTGATTCCGGAGGTCGTCGCCGTGGTGCTGGAGCGCCG
CCCCGACGATGCGCGCGCGTTCGTGATGCCGACTGCCTGCCCGGTATGCGGCTCGCACATCGAGCGCCTGGAAGACGAGG
CCATCGCGCGCTGCACGGGCGGCCTGATCTGTGCGGCGCAGCGCAAGCAGGCGCTGCTGCATTTCGCGCAGCGTCGCGCC
ATGGATATCGAAGGCCTTGGCGACAAGGTCGTCGAGCAGCTGGTCGACCAGGGCATCGTGCGCACGCCGGCGGATCTCTA
CAAACTGGGTGTGGCCAAGCTTGCCGCATTGGACCGCATGGCGGACAAGTCGGCGGGCAACCTCGTGGCTGCCATTGAAG
CCTCGCGCAGCACCACGTTCAACCGGTTCATCTTCTCGCTTGGAATTCGACACGTGGGCGAGGCTACGGCCAAGGACCTG
GCCCGCCATTTCGGCAAGCTCGACGGACTGATGGTGGCCGACGAGGCCGCGCTGCTTGAAGTCAACGACGTGGGCCCTGT
GGTGGCGCAGTCGATCGTCAATTTCTTCGGCGAGCCGCACAACGTCGAGGTCATCGAGCAGTTGCGCGCGGCAGGCGTAC
ACTGGCCGGAAAGCGAGCCCGTCGCGCGCGCGCCGGCACCACTCGCGGGCAAGACCTTCGTACTGACAGGCACGCTGCCG
AGCCTGTCGCGCGAGGCAGCCAAGGAAATGCTGGAGGCCGCGGGCGCCAAGGTCGCAGGCTCTGTATCGAAGAAGACAGA
CTACGTCGTGGCCGGCGCCGAGGCTGGCAGCAAGCTCGACAAGGCCGAGGCGCTGGGTGTACCGGTGCTCGACGAGGCAG
GAATGCTGGCGCTGCTGGAATCGGTCGGCGCGGGCCAACCGGGGGAGCAGTCATGA

Upstream 100 bases:

>100_bases
CAACCAGCTCGAGGCCCTCTACCAGAAGCTGGAAACGCGCGGGATGCCGGCCGGTTCGCCGGTGGCGGCACGGTTGTTCA
GCGTCTAGCGGGAGCCTGTC

Downstream 100 bases:

>100_bases
TCCGCGAGATTCTCAAGATGGGCGACGCGCGCCTGCTGCAGCAGGCGCGGCCGGTGGAGCGCTTCAATACGCCTGAACTG
CGCACGCTGATCGAAGACAT

Product: NAD-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 711; Mature: 711

Protein sequence:

>711_residues
MNATHRGAQADASAPAGGLPPATAAERVQWLRDELDRHNYQYYVLDAPTIPDAEYDALFTELQALEAEHPELLTPDSPTQ
RVGGAPLSAFDTVRHRVPMLSLNNGFSDEDVTGFDRRCAQGLGRSAAAGGEQDLFSAAEGVEYACELKFDGLAMSLRYED
GRLVQAATRGDGETGEDVTVNVRTIKAIPLKLRGAAPAVLEVRGEVFMYRRDFDKLNARQAEAGDKTFVNPRNAAAGSLR
QLDPRITAKRPLSFFAYGLGELQGIERPATHSAMLDGFATMGLPVCNERAVVKGAQGLLDFYRKVGEKRDQLPYDIDGVV
YKVNALAEQEQLGFVSRAPRFALAHKFPAQEMTTIVEDIEVQVGRTGAITPVARLQPVFVGGVTVTNATLHNEDEVRRKD
VHIGDTVIVRRAGDVIPEVVAVVLERRPDDARAFVMPTACPVCGSHIERLEDEAIARCTGGLICAAQRKQALLHFAQRRA
MDIEGLGDKVVEQLVDQGIVRTPADLYKLGVAKLAALDRMADKSAGNLVAAIEASRSTTFNRFIFSLGIRHVGEATAKDL
ARHFGKLDGLMVADEAALLEVNDVGPVVAQSIVNFFGEPHNVEVIEQLRAAGVHWPESEPVARAPAPLAGKTFVLTGTLP
SLSREAAKEMLEAAGAKVAGSVSKKTDYVVAGAEAGSKLDKAEALGVPVLDEAGMLALLESVGAGQPGEQS

Sequences:

>Translated_711_residues
MNATHRGAQADASAPAGGLPPATAAERVQWLRDELDRHNYQYYVLDAPTIPDAEYDALFTELQALEAEHPELLTPDSPTQ
RVGGAPLSAFDTVRHRVPMLSLNNGFSDEDVTGFDRRCAQGLGRSAAAGGEQDLFSAAEGVEYACELKFDGLAMSLRYED
GRLVQAATRGDGETGEDVTVNVRTIKAIPLKLRGAAPAVLEVRGEVFMYRRDFDKLNARQAEAGDKTFVNPRNAAAGSLR
QLDPRITAKRPLSFFAYGLGELQGIERPATHSAMLDGFATMGLPVCNERAVVKGAQGLLDFYRKVGEKRDQLPYDIDGVV
YKVNALAEQEQLGFVSRAPRFALAHKFPAQEMTTIVEDIEVQVGRTGAITPVARLQPVFVGGVTVTNATLHNEDEVRRKD
VHIGDTVIVRRAGDVIPEVVAVVLERRPDDARAFVMPTACPVCGSHIERLEDEAIARCTGGLICAAQRKQALLHFAQRRA
MDIEGLGDKVVEQLVDQGIVRTPADLYKLGVAKLAALDRMADKSAGNLVAAIEASRSTTFNRFIFSLGIRHVGEATAKDL
ARHFGKLDGLMVADEAALLEVNDVGPVVAQSIVNFFGEPHNVEVIEQLRAAGVHWPESEPVARAPAPLAGKTFVLTGTLP
SLSREAAKEMLEAAGAKVAGSVSKKTDYVVAGAEAGSKLDKAEALGVPVLDEAGMLALLESVGAGQPGEQS
>Mature_711_residues
MNATHRGAQADASAPAGGLPPATAAERVQWLRDELDRHNYQYYVLDAPTIPDAEYDALFTELQALEAEHPELLTPDSPTQ
RVGGAPLSAFDTVRHRVPMLSLNNGFSDEDVTGFDRRCAQGLGRSAAAGGEQDLFSAAEGVEYACELKFDGLAMSLRYED
GRLVQAATRGDGETGEDVTVNVRTIKAIPLKLRGAAPAVLEVRGEVFMYRRDFDKLNARQAEAGDKTFVNPRNAAAGSLR
QLDPRITAKRPLSFFAYGLGELQGIERPATHSAMLDGFATMGLPVCNERAVVKGAQGLLDFYRKVGEKRDQLPYDIDGVV
YKVNALAEQEQLGFVSRAPRFALAHKFPAQEMTTIVEDIEVQVGRTGAITPVARLQPVFVGGVTVTNATLHNEDEVRRKD
VHIGDTVIVRRAGDVIPEVVAVVLERRPDDARAFVMPTACPVCGSHIERLEDEAIARCTGGLICAAQRKQALLHFAQRRA
MDIEGLGDKVVEQLVDQGIVRTPADLYKLGVAKLAALDRMADKSAGNLVAAIEASRSTTFNRFIFSLGIRHVGEATAKDL
ARHFGKLDGLMVADEAALLEVNDVGPVVAQSIVNFFGEPHNVEVIEQLRAAGVHWPESEPVARAPAPLAGKTFVLTGTLP
SLSREAAKEMLEAAGAKVAGSVSKKTDYVVAGAEAGSKLDKAEALGVPVLDEAGMLALLESVGAGQPGEQS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=85, Percent_Identity=48.2352941176471, Blast_Score=77, Evalue=8e-14,
Organism=Escherichia coli, GI1788750, Length=676, Percent_Identity=59.3195266272189, Blast_Score=777, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=416, Percent_Identity=25.2403846153846, Blast_Score=86, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_CUPPJ (Q470D2)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296095.1
- ProteinModelPortal:   Q470D2
- GeneID:   3609565
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A1886
- NMPDR:   fig|264198.3.peg.2416
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK2503705
- BioCyc:   REUT264198:REUT_A1886-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 76290; Mature: 76290

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 148-148 BINDING 146-146 BINDING 169-169 BINDING 205-205 BINDING 322-322 BINDING 346-346

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATHRGAQADASAPAGGLPPATAAERVQWLRDELDRHNYQYYVLDAPTIPDAEYDALFT
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
ELQALEAEHPELLTPDSPTQRVGGAPLSAFDTVRHRVPMLSLNNGFSDEDVTGFDRRCAQ
HHHHHHCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHH
GLGRSAAAGGEQDLFSAAEGVEYACELKFDGLAMSLRYEDGRLVQAATRGDGETGEDVTV
HCCCCCCCCCHHHHHHHHCCCCEEEEEEECCEEEEEEECCCCEEEEEECCCCCCCCCEEE
NVRTIKAIPLKLRGAAPAVLEVRGEVFMYRRDFDKLNARQAEAGDKTFVNPRNAAAGSLR
EEEEEEEECEEECCCCCCEEEECCCEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCHH
QLDPRITAKRPLSFFAYGLGELQGIERPATHSAMLDGFATMGLPVCNERAVVKGAQGLLD
HCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
FYRKVGEKRDQLPYDIDGVVYKVNALAEQEQLGFVSRAPRFALAHKFPAQEMTTIVEDIE
HHHHHCCHHHCCCCCCCCEEEEEHHHHHHHHHCHHHCCCCHHHHHCCCHHHHHHHHHHHH
VQVGRTGAITPVARLQPVFVGGVTVTNATLHNEDEVRRKDVHIGDTVIVRRAGDVIPEVV
HEECCCCCCCHHHHCCEEEECCEEEEECEECCCHHHHHHCCCCCCEEEEEHHHHHHHHHH
AVVLERRPDDARAFVMPTACPVCGSHIERLEDEAIARCTGGLICAAQRKQALLHFAQRRA
HHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHC
MDIEGLGDKVVEQLVDQGIVRTPADLYKLGVAKLAALDRMADKSAGNLVAAIEASRSTTF
CCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHH
NRFIFSLGIRHVGEATAKDLARHFGKLDGLMVADEAALLEVNDVGPVVAQSIVNFFGEPH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCHHHHHHHHHHCCCCC
NVEVIEQLRAAGVHWPESEPVARAPAPLAGKTFVLTGTLPSLSREAAKEMLEAAGAKVAG
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHC
SVSKKTDYVVAGAEAGSKLDKAEALGVPVLDEAGMLALLESVGAGQPGEQS
CCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNATHRGAQADASAPAGGLPPATAAERVQWLRDELDRHNYQYYVLDAPTIPDAEYDALFT
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
ELQALEAEHPELLTPDSPTQRVGGAPLSAFDTVRHRVPMLSLNNGFSDEDVTGFDRRCAQ
HHHHHHCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHH
GLGRSAAAGGEQDLFSAAEGVEYACELKFDGLAMSLRYEDGRLVQAATRGDGETGEDVTV
HCCCCCCCCCHHHHHHHHCCCCEEEEEEECCEEEEEEECCCCEEEEEECCCCCCCCCEEE
NVRTIKAIPLKLRGAAPAVLEVRGEVFMYRRDFDKLNARQAEAGDKTFVNPRNAAAGSLR
EEEEEEEECEEECCCCCCEEEECCCEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCHH
QLDPRITAKRPLSFFAYGLGELQGIERPATHSAMLDGFATMGLPVCNERAVVKGAQGLLD
HCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
FYRKVGEKRDQLPYDIDGVVYKVNALAEQEQLGFVSRAPRFALAHKFPAQEMTTIVEDIE
HHHHHCCHHHCCCCCCCCEEEEEHHHHHHHHHCHHHCCCCHHHHHCCCHHHHHHHHHHHH
VQVGRTGAITPVARLQPVFVGGVTVTNATLHNEDEVRRKDVHIGDTVIVRRAGDVIPEVV
HEECCCCCCCHHHHCCEEEECCEEEEECEECCCHHHHHHCCCCCCEEEEEHHHHHHHHHH
AVVLERRPDDARAFVMPTACPVCGSHIERLEDEAIARCTGGLICAAQRKQALLHFAQRRA
HHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHC
MDIEGLGDKVVEQLVDQGIVRTPADLYKLGVAKLAALDRMADKSAGNLVAAIEASRSTTF
CCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHH
NRFIFSLGIRHVGEATAKDLARHFGKLDGLMVADEAALLEVNDVGPVVAQSIVNFFGEPH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCHHHHHHHHHHCCCCC
NVEVIEQLRAAGVHWPESEPVARAPAPLAGKTFVLTGTLPSLSREAAKEMLEAAGAKVAG
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHC
SVSKKTDYVVAGAEAGSKLDKAEALGVPVLDEAGMLALLESVGAGQPGEQS
CCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA