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The map label for this gene is rnhB

Identifier: 73541556

GI number: 73541556

Start: 2043300

End: 2043956

Strand: Reverse

Name: rnhB

Synonym: Reut_A1867

Alternate gene names: 73541556

Gene position: 2043956-2043300 (Counterclockwise)

Preceding gene: 73541557

Following gene: 73541555

Centisome position: 53.7

GC content: 66.36

Gene sequence:

>657_bases
ATGGCGCGCGGCAATCTTTCGTCGGCGCAAATGGGGCTTGACCTTGCGCCGGTGGCAGCAAGCGTCCGGTTCCTGTGCGG
TGTGGACGAGGCTGGCCGCGGGCCGTTGGCCGGGCCGGTGTATGCCGCCGCCGTGGTGTTCGATCCAGCGAAGCCAATGC
TCCGTGGGCTGGCGGATTCCAAGGTTCTCACCGCCAGCAAGCGTGAAGCGCTTTACGACAAGATATGCGAGCGCGCGCTC
GGCTGGCATATCGCCTTCGCCACGGTCGAGGAAATCGATACGCTGAACATCCTCCATGCCTCGATGCTTGCGATGCAACG
CGCGGTGCAGGGGCTGGCGGAGAAGGGCGTCACGCCGGATCTGGTCCAGGTGGATGGCAACCGCTGCCCGCAGGTGCCGT
TTCCGGTCGAGGCGATCGTCAAGGGCGACTCGCTGGTCAAGGCGATCTCGGCGGCGTCCATCCTGGCCAAGGTGGCGCGC
GACCGTGAGCTGCTGACGCTGCACGAGGTCTATCCGCAGTATGGCTTCGACGCACATTCCGGCTATCCGACACCGCAGCA
TCTTGCCGCACTGGCGCAATTCGGCGCCACGCCGCACCACCGGCGCTCGTTTGCGCCCGTGCGCGAGGCCATTGCCCGAG
GCCTGGTGGCCTTCTGA

Upstream 100 bases:

>100_bases
ACGAACACTTCACGCGCATGCACGAAACGCTCAAGTGCAATACGGCGCAGCTCGCCGCGGATGTGGTCGTCGACCTGATG
CGCTCGCGGGGGCTGGTCTG

Downstream 100 bases:

>100_bases
GCCGGGCGAACCGAGATCCACTCCTGTGAAGCACGTCACATCGCGCGACAACGCGCTGTTCAAACATCTTAAGGCTCTGG
CCACGTCGACACACCAGCGC

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MARGNLSSAQMGLDLAPVAASVRFLCGVDEAGRGPLAGPVYAAAVVFDPAKPMLRGLADSKVLTASKREALYDKICERAL
GWHIAFATVEEIDTLNILHASMLAMQRAVQGLAEKGVTPDLVQVDGNRCPQVPFPVEAIVKGDSLVKAISAASILAKVAR
DRELLTLHEVYPQYGFDAHSGYPTPQHLAALAQFGATPHHRRSFAPVREAIARGLVAF

Sequences:

>Translated_218_residues
MARGNLSSAQMGLDLAPVAASVRFLCGVDEAGRGPLAGPVYAAAVVFDPAKPMLRGLADSKVLTASKREALYDKICERAL
GWHIAFATVEEIDTLNILHASMLAMQRAVQGLAEKGVTPDLVQVDGNRCPQVPFPVEAIVKGDSLVKAISAASILAKVAR
DRELLTLHEVYPQYGFDAHSGYPTPQHLAALAQFGATPHHRRSFAPVREAIARGLVAF
>Mature_217_residues
ARGNLSSAQMGLDLAPVAASVRFLCGVDEAGRGPLAGPVYAAAVVFDPAKPMLRGLADSKVLTASKREALYDKICERALG
WHIAFATVEEIDTLNILHASMLAMQRAVQGLAEKGVTPDLVQVDGNRCPQVPFPVEAIVKGDSLVKAISAASILAKVARD
RELLTLHEVYPQYGFDAHSGYPTPQHLAALAQFGATPHHRRSFAPVREAIARGLVAF

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=190, Percent_Identity=58.421052631579, Blast_Score=218, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_CUPPJ (Q470F1)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296076.1
- ProteinModelPortal:   Q470F1
- SMR:   Q470F1
- GeneID:   3609254
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A1867
- NMPDR:   fig|264198.3.peg.2397
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   REUT264198:REUT_A1867-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 23247; Mature: 23116

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARGNLSSAQMGLDLAPVAASVRFLCGVDEAGRGPLAGPVYAAAVVFDPAKPMLRGLADS
CCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCC
KVLTASKREALYDKICERALGWHIAFATVEEIDTLNILHASMLAMQRAVQGLAEKGVTPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LVQVDGNRCPQVPFPVEAIVKGDSLVKAISAASILAKVARDRELLTLHEVYPQYGFDAHS
EEEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
GYPTPQHLAALAQFGATPHHRRSFAPVREAIARGLVAF
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARGNLSSAQMGLDLAPVAASVRFLCGVDEAGRGPLAGPVYAAAVVFDPAKPMLRGLADS
CCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCC
KVLTASKREALYDKICERALGWHIAFATVEEIDTLNILHASMLAMQRAVQGLAEKGVTPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LVQVDGNRCPQVPFPVEAIVKGDSLVKAISAASILAKVARDRELLTLHEVYPQYGFDAHS
EEEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
GYPTPQHLAALAQFGATPHHRRSFAPVREAIARGLVAF
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA