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The map label for this gene is yjfH [C]

Identifier: 73541555

GI number: 73541555

Start: 2042489

End: 2043274

Strand: Reverse

Name: yjfH [C]

Synonym: Reut_A1866

Alternate gene names: 73541555

Gene position: 2043274-2042489 (Counterclockwise)

Preceding gene: 73541556

Following gene: 73541553

Centisome position: 53.68

GC content: 65.39

Gene sequence:

>786_bases
GTGAAGCACGTCACATCGCGCGACAACGCGCTGTTCAAACATCTTAAGGCTCTGGCCACGTCGACACACCAGCGCCGCAA
GGCAGGGCAGTCGCTGCTCGATGGCGTGCATCTTGCCCAGGCCTATGTCGCGGCGCTCGGTCAGCCGGTGAGTTGCGTGG
TGTCGGAGCGGCACTACGATCATGCCGAGGTGGCACCGCTGCTGGCAGCGGTCGACAGCCAGCGGGTCGTGGTCCTGGCC
GATGTTTTGTTCACGCAGGTCAGCGGCGTGGTCAACGGCATCGATCTGATGCTCGTGATCGAGACGCCGTCTGGGCACTT
GCCGGCACGCATAGAAGAAGACTGCATTATCCTCGATGGGCTGCAGGATGCAGGGAATGTCGGATCCATCCTGCGCAGCG
CCGCAGCAGCCGGCATCCGCCACGCATTCCTCGGTACCGGTTGCGCGTTCGCGTGGTCGGTCAAGACCCTGCGCGCAGGC
ATGGGCGCCAACTTCCACCTGAATATTGTCGAGCACTGCACGATCGACATGCTCGCGCCGCGGCTTGCGGTGCCGTTGCT
GGCGACGTCGTCGCATGCGGATGCCGCAGTGTTCGACACCGACCTGCGAGGCCCCGTGGCCTGGATCGTCGGAAACGAAG
GCGCCGGCGTCAGTGACGCATGGATGGAGCACGTTGCGCGCACCGTCGGCATTCCTCAGCCGGGCGGCCTGGAGTCGCTG
AATGTGGGCGCCGCCACCGCGATCTGCCTGTTCGAGGCAGTGCGGCAGCGCCGCGGCACCCAGTAA

Upstream 100 bases:

>100_bases
GGCGCCACGCCGCACCACCGGCGCTCGTTTGCGCCCGTGCGCGAGGCCATTGCCCGAGGCCTGGTGGCCTTCTGAGCCGG
GCGAACCGAGATCCACTCCT

Downstream 100 bases:

>100_bases
CGGCAGTACGGCTCAGTAGTTGTCGCGGTCGACCTTGATTTCCTGAAGGATCGTCGTCGCGATTTCCTCGATGGACTTAT
GCGTCGAGGACAGCCACTTG

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKHVTSRDNALFKHLKALATSTHQRRKAGQSLLDGVHLAQAYVAALGQPVSCVVSERHYDHAEVAPLLAAVDSQRVVVLA
DVLFTQVSGVVNGIDLMLVIETPSGHLPARIEEDCIILDGLQDAGNVGSILRSAAAAGIRHAFLGTGCAFAWSVKTLRAG
MGANFHLNIVEHCTIDMLAPRLAVPLLATSSHADAAVFDTDLRGPVAWIVGNEGAGVSDAWMEHVARTVGIPQPGGLESL
NVGAATAICLFEAVRQRRGTQ

Sequences:

>Translated_261_residues
MKHVTSRDNALFKHLKALATSTHQRRKAGQSLLDGVHLAQAYVAALGQPVSCVVSERHYDHAEVAPLLAAVDSQRVVVLA
DVLFTQVSGVVNGIDLMLVIETPSGHLPARIEEDCIILDGLQDAGNVGSILRSAAAAGIRHAFLGTGCAFAWSVKTLRAG
MGANFHLNIVEHCTIDMLAPRLAVPLLATSSHADAAVFDTDLRGPVAWIVGNEGAGVSDAWMEHVARTVGIPQPGGLESL
NVGAATAICLFEAVRQRRGTQ
>Mature_261_residues
MKHVTSRDNALFKHLKALATSTHQRRKAGQSLLDGVHLAQAYVAALGQPVSCVVSERHYDHAEVAPLLAAVDSQRVVVLA
DVLFTQVSGVVNGIDLMLVIETPSGHLPARIEEDCIILDGLQDAGNVGSILRSAAAAGIRHAFLGTGCAFAWSVKTLRAG
MGANFHLNIVEHCTIDMLAPRLAVPLLATSSHADAAVFDTDLRGPVAWIVGNEGAGVSDAWMEHVARTVGIPQPGGLESL
NVGAATAICLFEAVRQRRGTQ

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=242, Percent_Identity=30.5785123966942, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27527; Mature: 27527

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHVTSRDNALFKHLKALATSTHQRRKAGQSLLDGVHLAQAYVAALGQPVSCVVSERHYD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCC
HAEVAPLLAAVDSQRVVVLADVLFTQVSGVVNGIDLMLVIETPSGHLPARIEEDCIILDG
HHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCEEEEEC
LQDAGNVGSILRSAAAAGIRHAFLGTGCAFAWSVKTLRAGMGANFHLNIVEHCTIDMLAP
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHH
RLAVPLLATSSHADAAVFDTDLRGPVAWIVGNEGAGVSDAWMEHVARTVGIPQPGGLESL
HHHHHHHCCCCCCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
NVGAATAICLFEAVRQRRGTQ
CCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKHVTSRDNALFKHLKALATSTHQRRKAGQSLLDGVHLAQAYVAALGQPVSCVVSERHYD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCC
HAEVAPLLAAVDSQRVVVLADVLFTQVSGVVNGIDLMLVIETPSGHLPARIEEDCIILDG
HHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCEEEEEC
LQDAGNVGSILRSAAAAGIRHAFLGTGCAFAWSVKTLRAGMGANFHLNIVEHCTIDMLAP
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHH
RLAVPLLATSSHADAAVFDTDLRGPVAWIVGNEGAGVSDAWMEHVARTVGIPQPGGLESL
HHHHHHHCCCCCCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
NVGAATAICLFEAVRQRRGTQ
CCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]