BacMap

Definition	Ralstonia eutropha JMP134 chromosome 1, complete sequence.
Accession	NC_007347
Length	3,806,533

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The map label for this gene is ykvO [H]

Identifier: 73541241

GI number: 73541241

Start: 1671370

End: 1672218

Strand: Direct

Name: ykvO [H]

Synonym: Reut_A1551

Alternate gene names: 73541241

Gene position: 1671370-1672218 (Clockwise)

Preceding gene: 73541240

Following gene: 73541242

Centisome position: 43.91

GC content: 69.85

Gene sequence:

>849_bases
ATGACGAAAGCATTGGAAGGTAAGGCAGCACTGGTTACCGGCGGCGCAGGCGGGATTGGCGCGGCGAGCGCGTTGGCACT
GGCGCGCGACGGCGCCGCGGTGGCATTGATGGGCCGGCGGCGCGAAGCGCTGGAAGTTGCCCGGGACGCGCTGCGCGAGA
AGGTGCCCGGCGCCAGCATCGAGATCTGCGTGGGCGATGCATGCCGCGCCGACGATGTGCAGGCCGCATTGCAGCGGGCG
TGGGACCTGCGCGGGCGGCTCGATATCCTCGTGCCAACGGTCGGTGGCGGCGGCTTCAAGCCGATGCTGATGCATGACGC
TGCTTCGTTCATGGCCGAGATCGAGTTCAACATCGCCAGCGCGTTCCTGGCCGTGCGCCACGCCGCGCCGTTGATGGCGC
CGCACGGCGGCAGCATCGTCTGCATTTCGTCGGGCGCGGCGCGCATGAACTGCCGCTGGCTGTCGGCCTACTGTGCGGCC
AAGGCCGGGCTGGAGGCGTTCGTGCGCGGCGCCGCGGAGGAACTGGCCGAAGCCGGCATCCGTGTCAACGCAGTGCGGCC
GGGGCTCACACGCTCGGGCGCGACTGGCCCGATGTTCGACAACGAAGCGGTGCTGGGCCAGTTTGTCGCGCAGATGCCGC
TGGGCCGCGCGGGTGAGCCGGAGGACATCGCCAACGCCGTGCGTTACCTCGCTGGGCCGGAATCCGGATGGGTGACGGGA
CAGAGCTTTGGCGTCGATGGCGGCAACGAGCTGCGCGGCAACCCCATGCTGGACGATGCCATCGCTGCCATGTACGGCAG
GGCCGCGCTGGACGACGTGCTTGCCGGCAGGATTCCGGCCGCGGCATGA

Upstream 100 bases:

>100_bases
AGGACCGCTATGTGCGCCGTCAGGGCGAGTGGCGAATCGCCGCGCGCACGCTGCTTGTCGACTGGACCCAGGATCTCCCA
CAGAAAGGATGAGGCACAGC

Downstream 100 bases:

>100_bases
ACGGCCGGCACAACGAAGGAGGAGAACGCAGATGAAGCAAGAAGGTAGGGTGAAGGGCAAAGTCGCCCTGGTCACCGGCG
CGGCCAGCGGCGTCGGCCGT

Product: Short-chain dehydrogenase/reductase SDR

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTKALEGKAALVTGGAGGIGAASALALARDGAAVALMGRRREALEVARDALREKVPGASIEICVGDACRADDVQAALQRA
WDLRGRLDILVPTVGGGGFKPMLMHDAASFMAEIEFNIASAFLAVRHAAPLMAPHGGSIVCISSGAARMNCRWLSAYCAA
KAGLEAFVRGAAEELAEAGIRVNAVRPGLTRSGATGPMFDNEAVLGQFVAQMPLGRAGEPEDIANAVRYLAGPESGWVTG
QSFGVDGGNELRGNPMLDDAIAAMYGRAALDDVLAGRIPAAA

Sequences:

>Translated_282_residues
MTKALEGKAALVTGGAGGIGAASALALARDGAAVALMGRRREALEVARDALREKVPGASIEICVGDACRADDVQAALQRA
WDLRGRLDILVPTVGGGGFKPMLMHDAASFMAEIEFNIASAFLAVRHAAPLMAPHGGSIVCISSGAARMNCRWLSAYCAA
KAGLEAFVRGAAEELAEAGIRVNAVRPGLTRSGATGPMFDNEAVLGQFVAQMPLGRAGEPEDIANAVRYLAGPESGWVTG
QSFGVDGGNELRGNPMLDDAIAAMYGRAALDDVLAGRIPAAA
>Mature_281_residues
TKALEGKAALVTGGAGGIGAASALALARDGAAVALMGRRREALEVARDALREKVPGASIEICVGDACRADDVQAALQRAW
DLRGRLDILVPTVGGGGFKPMLMHDAASFMAEIEFNIASAFLAVRHAAPLMAPHGGSIVCISSGAARMNCRWLSAYCAAK
AGLEAFVRGAAEELAEAGIRVNAVRPGLTRSGATGPMFDNEAVLGQFVAQMPLGRAGEPEDIANAVRYLAGPESGWVTGQ
SFGVDGGNELRGNPMLDDAIAAMYGRAALDDVLAGRIPAAA

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI5031737, Length=244, Percent_Identity=30.7377049180328, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI19923817, Length=268, Percent_Identity=30.2238805970149, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI32483357, Length=250, Percent_Identity=30.4, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI15277342, Length=263, Percent_Identity=30.7984790874525, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI40254992, Length=245, Percent_Identity=30.2040816326531, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI7705925, Length=252, Percent_Identity=29.3650793650794, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI31542939, Length=232, Percent_Identity=30.1724137931034, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI126723750, Length=253, Percent_Identity=28.4584980237154, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI10190704, Length=256, Percent_Identity=30.859375, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI66933014, Length=256, Percent_Identity=28.90625, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4503301, Length=252, Percent_Identity=24.2063492063492, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI59889578, Length=210, Percent_Identity=27.6190476190476, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI33667109, Length=189, Percent_Identity=30.6878306878307, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI224922801, Length=172, Percent_Identity=31.3953488372093, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787905, Length=245, Percent_Identity=33.469387755102, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789378, Length=250, Percent_Identity=33.2, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI87082160, Length=250, Percent_Identity=29.2, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787335, Length=245, Percent_Identity=33.0612244897959, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI2367365, Length=248, Percent_Identity=33.0645161290323, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082100, Length=262, Percent_Identity=32.824427480916, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789208, Length=247, Percent_Identity=29.1497975708502, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790717, Length=249, Percent_Identity=26.1044176706827, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788459, Length=254, Percent_Identity=31.8897637795276, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786812, Length=256, Percent_Identity=30.078125, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1789057, Length=260, Percent_Identity=28.4615384615385, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI2367175, Length=250, Percent_Identity=30.8, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787526, Length=250, Percent_Identity=27.2, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=262, Percent_Identity=31.6793893129771, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17562990, Length=265, Percent_Identity=30.9433962264151, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17559104, Length=265, Percent_Identity=32.0754716981132, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI72000259, Length=259, Percent_Identity=31.6602316602317, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560332, Length=262, Percent_Identity=32.4427480916031, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17562906, Length=265, Percent_Identity=32.0754716981132, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17562904, Length=281, Percent_Identity=28.8256227758007, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17560220, Length=272, Percent_Identity=30.5147058823529, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17551412, Length=267, Percent_Identity=30.3370786516854, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560676, Length=254, Percent_Identity=29.9212598425197, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17538480, Length=269, Percent_Identity=29.7397769516729, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17563726, Length=257, Percent_Identity=29.9610894941634, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17562910, Length=263, Percent_Identity=31.9391634980989, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17565030, Length=269, Percent_Identity=29.7397769516729, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17560150, Length=259, Percent_Identity=29.3436293436293, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI193204405, Length=265, Percent_Identity=32.4528301886792, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI25147288, Length=251, Percent_Identity=34.2629482071713, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17562908, Length=272, Percent_Identity=31.6176470588235, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17538486, Length=261, Percent_Identity=29.8850574712644, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71994604, Length=249, Percent_Identity=29.718875502008, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17555706, Length=249, Percent_Identity=32.9317269076305, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71994600, Length=245, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17561402, Length=258, Percent_Identity=29.4573643410853, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17536025, Length=256, Percent_Identity=28.90625, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17536651, Length=256, Percent_Identity=29.6875, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17544670, Length=274, Percent_Identity=27.3722627737226, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115534694, Length=245, Percent_Identity=28.1632653061224, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17561272, Length=254, Percent_Identity=27.9527559055118, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=263, Percent_Identity=27.3764258555133, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=246, Percent_Identity=26.8292682926829, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323882, Length=198, Percent_Identity=28.2828282828283, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI23397609, Length=251, Percent_Identity=33.0677290836653, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21355319, Length=256, Percent_Identity=32.03125, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24644339, Length=249, Percent_Identity=32.1285140562249, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24639444, Length=255, Percent_Identity=32.9411764705882, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21357041, Length=254, Percent_Identity=29.1338582677165, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28571526, Length=249, Percent_Identity=28.5140562248996, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24643142, Length=252, Percent_Identity=31.3492063492063, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24644337, Length=256, Percent_Identity=31.640625, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17737361, Length=260, Percent_Identity=31.1538461538462, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 28899; Mature: 28768

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKALEGKAALVTGGAGGIGAASALALARDGAAVALMGRRREALEVARDALREKVPGASI
CCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEE
EICVGDACRADDVQAALQRAWDLRGRLDILVPTVGGGGFKPMLMHDAASFMAEIEFNIAS
EEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
AFLAVRHAAPLMAPHGGSIVCISSGAARMNCRWLSAYCAAKAGLEAFVRGAAEELAEAGI
HHHHHHHHCCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RVNAVRPGLTRSGATGPMFDNEAVLGQFVAQMPLGRAGEPEDIANAVRYLAGPESGWVTG
EEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
QSFGVDGGNELRGNPMLDDAIAAMYGRAALDDVLAGRIPAAA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TKALEGKAALVTGGAGGIGAASALALARDGAAVALMGRRREALEVARDALREKVPGASI
CCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEE
EICVGDACRADDVQAALQRAWDLRGRLDILVPTVGGGGFKPMLMHDAASFMAEIEFNIAS
EEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
AFLAVRHAAPLMAPHGGSIVCISSGAARMNCRWLSAYCAAKAGLEAFVRGAAEELAEAGI
HHHHHHHHCCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RVNAVRPGLTRSGATGPMFDNEAVLGQFVAQMPLGRAGEPEDIANAVRYLAGPESGWVTG
EEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
QSFGVDGGNELRGNPMLDDAIAAMYGRAALDDVLAGRIPAAA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]