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The map label for this gene is cysD [H]

Identifier: 73541221

GI number: 73541221

Start: 1649728

End: 1650624

Strand: Reverse

Name: cysD [H]

Synonym: Reut_A1531

Alternate gene names: 73541221

Gene position: 1650624-1649728 (Counterclockwise)

Preceding gene: 73541226

Following gene: 73541220

Centisome position: 43.36

GC content: 63.55

Gene sequence:

>897_bases
ATGTTGACCCACCTGGAGCAACTGGAGGCGGAGAGCATCCACATCATGCGGGAAGTGGTGGCGGAATGCGAAAACCCCGT
CATGCTCTATTCCATCGGCAAGGACAGCTCCGTCATGCTGCACCTGGCGATGAAGGCCTTCCACCCGGCCCGGCCGCCCT
TTCCGCTACTGCACGTCGACACGACGTGGAAGTTCCGCGAAATGATCGAGTTCCGCGACCAGACTGTCGAACGGCTCGGG
CTCGACCTGCGCGTGCATATCAATCCGGAAGGCATCGCGCGGAACATCAGCCCGCACGAACACGGCTCGGCCGTGCACAC
CGACACCTGGAAGACGCAGGGGCTCAAGCAGGCGCTCGACCACTATGGCTTTGATGCCGCGTTCGGCGGTGCGCGTCGCG
ACGAAGAGAAATCGCGCGCCAAGGAGCGTGTGTTCTCGGTGCGCACTGCGCAGCACCGCTGGGACCCGAAGATGCAACGC
CCCGAACTCTGGCGCCAGTACAACGCCCGCAAGCGCAAGGGCGAAAGCCTGCGCGTGTTCCCGCTGTCGAACTGGACCGA
GCTCGACATCTGGCAATACATCTACCTGAACAAGATACCGATCGTGCCCCTGTACTACGCCAAGGAGCGTCCCGTGGTCG
AGCGCGACGGCACGATGATCATGGTCGATGACGAACGCCTGCCGCTGCGCCCGGGCGAGATGCCGGCCCTGCGCAAAGTG
CGTTTCCGCACGCTGGGCTGCTATCCGCTCACCGGCGCGATCGACAGCGAAGCCGACACGCTCGGCGGGATCATCCAGGA
AATGCTGCTGGCCACCACGTCGGAGCGCCAGGGGCGGCTGATCGACAGCGATTCGGCCGGGTCGATGGAGAAGAAAAAGC
AAGAGGGGTACTTCTGA

Upstream 100 bases:

>100_bases
CCCGGACGAAGTTGCGCAAGGCCTCGGCTCGTTACCTTTTCCCCATGCCAGGCACCGCGAAATCCGTGGACGGCGCCGGC
AACACGAAGAGGAGATCTCG

Downstream 100 bases:

>100_bases
TGGAAGCGCTGATGGCAATGCAGACGGAAACCGTGCCGGTCACGGCCAATGACGACATCGGGCAATACCTGCGTGCGCAG
CAACACAAGGGCCTGCTGCG

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MLTHLEQLEAESIHIMREVVAECENPVMLYSIGKDSSVMLHLAMKAFHPARPPFPLLHVDTTWKFREMIEFRDQTVERLG
LDLRVHINPEGIARNISPHEHGSAVHTDTWKTQGLKQALDHYGFDAAFGGARRDEEKSRAKERVFSVRTAQHRWDPKMQR
PELWRQYNARKRKGESLRVFPLSNWTELDIWQYIYLNKIPIVPLYYAKERPVVERDGTMIMVDDERLPLRPGEMPALRKV
RFRTLGCYPLTGAIDSEADTLGGIIQEMLLATTSERQGRLIDSDSAGSMEKKKQEGYF

Sequences:

>Translated_298_residues
MLTHLEQLEAESIHIMREVVAECENPVMLYSIGKDSSVMLHLAMKAFHPARPPFPLLHVDTTWKFREMIEFRDQTVERLG
LDLRVHINPEGIARNISPHEHGSAVHTDTWKTQGLKQALDHYGFDAAFGGARRDEEKSRAKERVFSVRTAQHRWDPKMQR
PELWRQYNARKRKGESLRVFPLSNWTELDIWQYIYLNKIPIVPLYYAKERPVVERDGTMIMVDDERLPLRPGEMPALRKV
RFRTLGCYPLTGAIDSEADTLGGIIQEMLLATTSERQGRLIDSDSAGSMEKKKQEGYF
>Mature_298_residues
MLTHLEQLEAESIHIMREVVAECENPVMLYSIGKDSSVMLHLAMKAFHPARPPFPLLHVDTTWKFREMIEFRDQTVERLG
LDLRVHINPEGIARNISPHEHGSAVHTDTWKTQGLKQALDHYGFDAAFGGARRDEEKSRAKERVFSVRTAQHRWDPKMQR
PELWRQYNARKRKGESLRVFPLSNWTELDIWQYIYLNKIPIVPLYYAKERPVVERDGTMIMVDDERLPLRPGEMPALRKV
RFRTLGCYPLTGAIDSEADTLGGIIQEMLLATTSERQGRLIDSDSAGSMEKKKQEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=297, Percent_Identity=71.7171717171717, Blast_Score=442, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 34528; Mature: 34528

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTHLEQLEAESIHIMREVVAECENPVMLYSIGKDSSVMLHLAMKAFHPARPPFPLLHVD
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEC
TTWKFREMIEFRDQTVERLGLDLRVHINPEGIARNISPHEHGSAVHTDTWKTQGLKQALD
CCHHHHHHHHHHHHHHHHHCCEEEEEECHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHH
HYGFDAAFGGARRDEEKSRAKERVFSVRTAQHRWDPKMQRPELWRQYNARKRKGESLRVF
HCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEE
PLSNWTELDIWQYIYLNKIPIVPLYYAKERPVVERDGTMIMVDDERLPLRPGEMPALRKV
ECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEECCCCEEEEECCCCCCCCCCCHHHHHH
RFRTLGCYPLTGAIDSEADTLGGIIQEMLLATTSERQGRLIDSDSAGSMEKKKQEGYF
HHHEECCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MLTHLEQLEAESIHIMREVVAECENPVMLYSIGKDSSVMLHLAMKAFHPARPPFPLLHVD
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEC
TTWKFREMIEFRDQTVERLGLDLRVHINPEGIARNISPHEHGSAVHTDTWKTQGLKQALD
CCHHHHHHHHHHHHHHHHHCCEEEEEECHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHH
HYGFDAAFGGARRDEEKSRAKERVFSVRTAQHRWDPKMQRPELWRQYNARKRKGESLRVF
HCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEE
PLSNWTELDIWQYIYLNKIPIVPLYYAKERPVVERDGTMIMVDDERLPLRPGEMPALRKV
ECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEECCCCEEEEECCCCCCCCCCCHHHHHH
RFRTLGCYPLTGAIDSEADTLGGIIQEMLLATTSERQGRLIDSDSAGSMEKKKQEGYF
HHHEECCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA