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The map label for this gene is cysL [H]

Identifier: 73541198

GI number: 73541198

Start: 1625935

End: 1626849

Strand: Reverse

Name: cysL [H]

Synonym: Reut_A1507

Alternate gene names: 73541198

Gene position: 1626849-1625935 (Counterclockwise)

Preceding gene: 73541199

Following gene: 73541197

Centisome position: 42.74

GC content: 64.92

Gene sequence:

>915_bases
ATGCCGGTTCCCTTTGACATCACCGATTTCCGCCTGTTCGTCAACGTTGCGGAAACGCGCAGCCTGACCCGCGGTGCCGA
GCGATCGTTCCTGTCCGTGCCGGCCGTCAGCAACCGGATCAAGAACCTCGAAGATACGCTGGGCTTGCGCTTGCTGGAAC
GCTCGCCGCAGGGGGTGACGCTGACCGCCGCCGGCGAGGTCTATCTGCAGCACGCGCGCGTGGTGCTGACGGAACTGGAA
CGCCTGACCGGAAACCTGCAACCGTTCACCACGGGCCTGAGCGGGCAGCTCAAACTGGTGGCCAACACCACGGCCATTAC
GGAATACCTGCCGCCGGTCATCGGCGACTACCTGGCCACCCACCCCGATGTGCGCATTGAAGTGCGCGAGCGCCTGAGCG
ACGATATCGTGCGCCTGGTACGCGAAGGCGGTACGGATCTCGGCATCATCTCGGGCAACATCCCGACCCAGGGCCTGCAA
TCGGTACCGTTCGTCAAGAGTTCGCTGATCGTGGTCGCTCCGCTGGGCCACCCGCTCCTGGCCGAGCGGGACGTGTGGTT
CGGGCAAGTCCTGGACCACGCCTTTGTCGCGCTGCTCGAAGGCAGCGCGTTCCAGATGTTCATGACGCGCGCGGCCGGCT
CTCTGCACAAGCCCATGACCATCCGCGTGCATGTCGCAAGCTACGACGCCATCTGCCGCATGGTGGCCGCGGGCGCCGGG
ATTGCCATCATGCCAAAGGCAGCATTCGTGCGCCTGAAGGGCCAGCAGCAGATTGGCGCTTGCAACCTGCGCGATGACTG
GGCCGTGCGCACGTTCCAGGTTTGCGCGCGCGATCTGGACGGCCTGTCCAGCTTTGCACGTGAATTCGCCAATCTGCTGA
TCGGCCACTACGCGCCCGGCGCGGAAGGAAGCTAG

Upstream 100 bases:

>100_bases
CCCGCGCCATGCGGCAGCGCGTTACAGGCAGTTGGCGGCGATTCCGCGGTGACGCGGCTATCATCGCGGGTCAGTGAATG
TTCGCCGCGAGATTCGAAGA

Downstream 100 bases:

>100_bases
CCCATGCGCCGGCCTCGCGCCGGTGGACTTAAGCCAGCGCTAAGGGCGCTTGCAGAAGTCGAAATTGTGAACCGCCGGGC
AGCGTGGCAACCTTGTTTCG

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MPVPFDITDFRLFVNVAETRSLTRGAERSFLSVPAVSNRIKNLEDTLGLRLLERSPQGVTLTAAGEVYLQHARVVLTELE
RLTGNLQPFTTGLSGQLKLVANTTAITEYLPPVIGDYLATHPDVRIEVRERLSDDIVRLVREGGTDLGIISGNIPTQGLQ
SVPFVKSSLIVVAPLGHPLLAERDVWFGQVLDHAFVALLEGSAFQMFMTRAAGSLHKPMTIRVHVASYDAICRMVAAGAG
IAIMPKAAFVRLKGQQQIGACNLRDDWAVRTFQVCARDLDGLSSFAREFANLLIGHYAPGAEGS

Sequences:

>Translated_304_residues
MPVPFDITDFRLFVNVAETRSLTRGAERSFLSVPAVSNRIKNLEDTLGLRLLERSPQGVTLTAAGEVYLQHARVVLTELE
RLTGNLQPFTTGLSGQLKLVANTTAITEYLPPVIGDYLATHPDVRIEVRERLSDDIVRLVREGGTDLGIISGNIPTQGLQ
SVPFVKSSLIVVAPLGHPLLAERDVWFGQVLDHAFVALLEGSAFQMFMTRAAGSLHKPMTIRVHVASYDAICRMVAAGAG
IAIMPKAAFVRLKGQQQIGACNLRDDWAVRTFQVCARDLDGLSSFAREFANLLIGHYAPGAEGS
>Mature_303_residues
PVPFDITDFRLFVNVAETRSLTRGAERSFLSVPAVSNRIKNLEDTLGLRLLERSPQGVTLTAAGEVYLQHARVVLTELER
LTGNLQPFTTGLSGQLKLVANTTAITEYLPPVIGDYLATHPDVRIEVRERLSDDIVRLVREGGTDLGIISGNIPTQGLQS
VPFVKSSLIVVAPLGHPLLAERDVWFGQVLDHAFVALLEGSAFQMFMTRAAGSLHKPMTIRVHVASYDAICRMVAAGAGI
AIMPKAAFVRLKGQQQIGACNLRDDWAVRTFQVCARDLDGLSSFAREFANLLIGHYAPGAEGS

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=292, Percent_Identity=23.6301369863014, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI157672245, Length=186, Percent_Identity=29.5698924731183, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787879, Length=256, Percent_Identity=26.953125, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788887, Length=244, Percent_Identity=26.6393442622951, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787601, Length=202, Percent_Identity=30.6930693069307, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788748, Length=262, Percent_Identity=27.0992366412214, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI2367136, Length=241, Percent_Identity=27.3858921161826, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=27.0916334661355, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693193, Length=169, Percent_Identity=32.5443786982249, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790399, Length=247, Percent_Identity=25.9109311740891, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787806, Length=266, Percent_Identity=24.0601503759398, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788297, Length=253, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786713, Length=115, Percent_Identity=39.1304347826087, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081904, Length=195, Percent_Identity=34.8717948717949, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786401, Length=237, Percent_Identity=24.0506329113924, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787589, Length=258, Percent_Identity=27.5193798449612, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789173, Length=176, Percent_Identity=28.9772727272727, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787128, Length=157, Percent_Identity=27.3885350318471, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786448, Length=174, Percent_Identity=29.3103448275862, Blast_Score=61, Evalue=8e-11,
Organism=Escherichia coli, GI1789204, Length=241, Percent_Identity=21.1618257261411, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33060; Mature: 32928

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVPFDITDFRLFVNVAETRSLTRGAERSFLSVPAVSNRIKNLEDTLGLRLLERSPQGVT
CCCCCCCHHHHEEEEHHHHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEE
LTAAGEVYLQHARVVLTELERLTGNLQPFTTGLSGQLKLVANTTAITEYLPPVIGDYLAT
EEECHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHC
HPDVRIEVRERLSDDIVRLVREGGTDLGIISGNIPTQGLQSVPFVKSSLIVVAPLGHPLL
CCCEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCH
AERDVWFGQVLDHAFVALLEGSAFQMFMTRAAGSLHKPMTIRVHVASYDAICRMVAAGAG
HCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHCCCC
IAIMPKAAFVRLKGQQQIGACNLRDDWAVRTFQVCARDLDGLSSFAREFANLLIGHYAPG
EEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AEGS
CCCC
>Mature Secondary Structure 
PVPFDITDFRLFVNVAETRSLTRGAERSFLSVPAVSNRIKNLEDTLGLRLLERSPQGVT
CCCCCCHHHHEEEEHHHHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEE
LTAAGEVYLQHARVVLTELERLTGNLQPFTTGLSGQLKLVANTTAITEYLPPVIGDYLAT
EEECHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHC
HPDVRIEVRERLSDDIVRLVREGGTDLGIISGNIPTQGLQSVPFVKSSLIVVAPLGHPLL
CCCEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCH
AERDVWFGQVLDHAFVALLEGSAFQMFMTRAAGSLHKPMTIRVHVASYDAICRMVAAGAG
HCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHCCCC
IAIMPKAAFVRLKGQQQIGACNLRDDWAVRTFQVCARDLDGLSSFAREFANLLIGHYAPG
EEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AEGS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]