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The map label for this gene is yfcG [H]

Identifier: 73541179

GI number: 73541179

Start: 1607999

End: 1608613

Strand: Direct

Name: yfcG [H]

Synonym: Reut_A1486

Alternate gene names: 73541179

Gene position: 1607999-1608613 (Clockwise)

Preceding gene: 73541178

Following gene: 73541180

Centisome position: 42.24

GC content: 58.86

Gene sequence:

>615_bases
ATGATTGATCTCTATTCGTGGAGTACGCCGAACGGTCGAAAAATATCGATCGCGCTTGAGGAGCTCAACCTGGGTTACAC
ATTGCATCCGGTCGACATCTCGAAGGGCGAGCAGTTCAGCGCGGAGTTTCTGGCGATGAATCCGAACAATAAGATTCCTG
TCATCCGCGACAGCGATGGGGTGGACGGGCATCCGGTCACGCTGTTCGAGTCGGGTGCGATTCTCGTTTATCTCGCTGAC
AAGGCCCGGCGCCTGCTGCCGGTAGCGGGGCGCGCACGGTATGAAACAATCCAGTGGCTGATGTTCCAGATGGGCGGCGT
GGGTCCGATGTTCGGACAAACGCATCACTTCCAGCGTTACGCCGCGCACGAGAAGTACAGCCTTGAGCGCTACACCACCG
AGACCCATCGGCTATACAAGGTGCTCGACGGCCAGCTCGCGAAAAGCGAGTTCGTGGGTGGCGACGCGTACAGCATTGCC
GATATCGCGATCTATCCGTGGGTTGCGCGCTTCGGTCTGCATCAGATCGACCTGGCTTCTGTGCCGCACGTCAAACGCTG
GTATGACACCTTGGGTGAACGGCCGGCGGTGCAGCGTGGCATGGAGGTGCTGTGA

Upstream 100 bases:

>100_bases
ATGTGCCGGCCAGCGCCGGCAATCCGGCGGCACGCTAACGTCGTAGCCGTATTCAAGATATCGCGCTCCTCGAGCGCCTA
GTCATTCTGGAGAACGAAAC

Downstream 100 bases:

>100_bases
TGACCAGCCATTCGACCCTGACTGTGCTTGGACGTGCGAACTCTTCTAACGTACAAAAAGTGATGTGGCTGCTGGCCGAA
ATCGGCCAGTCGTGCCATCG

Product: glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MIDLYSWSTPNGRKISIALEELNLGYTLHPVDISKGEQFSAEFLAMNPNNKIPVIRDSDGVDGHPVTLFESGAILVYLAD
KARRLLPVAGRARYETIQWLMFQMGGVGPMFGQTHHFQRYAAHEKYSLERYTTETHRLYKVLDGQLAKSEFVGGDAYSIA
DIAIYPWVARFGLHQIDLASVPHVKRWYDTLGERPAVQRGMEVL

Sequences:

>Translated_204_residues
MIDLYSWSTPNGRKISIALEELNLGYTLHPVDISKGEQFSAEFLAMNPNNKIPVIRDSDGVDGHPVTLFESGAILVYLAD
KARRLLPVAGRARYETIQWLMFQMGGVGPMFGQTHHFQRYAAHEKYSLERYTTETHRLYKVLDGQLAKSEFVGGDAYSIA
DIAIYPWVARFGLHQIDLASVPHVKRWYDTLGERPAVQRGMEVL
>Mature_204_residues
MIDLYSWSTPNGRKISIALEELNLGYTLHPVDISKGEQFSAEFLAMNPNNKIPVIRDSDGVDGHPVTLFESGAILVYLAD
KARRLLPVAGRARYETIQWLMFQMGGVGPMFGQTHHFQRYAAHEKYSLERYTTETHRLYKVLDGQLAKSEFVGGDAYSIA
DIAIYPWVARFGLHQIDLASVPHVKRWYDTLGERPAVQRGMEVL

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=203, Percent_Identity=51.2315270935961, Blast_Score=201, Evalue=2e-53,
Organism=Escherichia coli, GI87082195, Length=213, Percent_Identity=44.131455399061, Blast_Score=159, Evalue=1e-40,
Organism=Escherichia coli, GI87081789, Length=175, Percent_Identity=32, Blast_Score=80, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324100, Length=237, Percent_Identity=32.4894514767932, Blast_Score=99, Evalue=5e-22,
Organism=Drosophila melanogaster, GI28571670, Length=171, Percent_Identity=30.4093567251462, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17864592, Length=183, Percent_Identity=30.6010928961749, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17864598, Length=184, Percent_Identity=29.3478260869565, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17864594, Length=187, Percent_Identity=28.8770053475936, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19922160, Length=201, Percent_Identity=26.865671641791, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17933730, Length=206, Percent_Identity=28.1553398058252, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20129811, Length=197, Percent_Identity=28.4263959390863, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI85725204, Length=197, Percent_Identity=26.3959390862944, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17737923, Length=197, Percent_Identity=26.3959390862944, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646249, Length=166, Percent_Identity=30.7228915662651, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 23113; Mature: 23113

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLYSWSTPNGRKISIALEELNLGYTLHPVDISKGEQFSAEFLAMNPNNKIPVIRDSDG
CCEECCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCCEEEEEECCCCCCCEEECCCC
VDGHPVTLFESGAILVYLADKARRLLPVAGRARYETIQWLMFQMGGVGPMFGQTHHFQRY
CCCCCEEEEECCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
AAHEKYSLERYTTETHRLYKVLDGQLAKSEFVGGDAYSIADIAIYPWVARFGLHQIDLAS
HHHCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEECCCC
VPHVKRWYDTLGERPAVQRGMEVL
CCHHHHHHHHHCCCHHHHCCCCCC
>Mature Secondary Structure
MIDLYSWSTPNGRKISIALEELNLGYTLHPVDISKGEQFSAEFLAMNPNNKIPVIRDSDG
CCEECCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCCEEEEEECCCCCCCEEECCCC
VDGHPVTLFESGAILVYLADKARRLLPVAGRARYETIQWLMFQMGGVGPMFGQTHHFQRY
CCCCCEEEEECCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
AAHEKYSLERYTTETHRLYKVLDGQLAKSEFVGGDAYSIADIAIYPWVARFGLHQIDLAS
HHHCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEECCCC
VPHVKRWYDTLGERPAVQRGMEVL
CCHHHHHHHHHCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]