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The map label for this gene is holB [H]

Identifier: 73541129

GI number: 73541129

Start: 1550227

End: 1551240

Strand: Direct

Name: holB [H]

Synonym: Reut_A1435

Alternate gene names: 73541129

Gene position: 1550227-1551240 (Clockwise)

Preceding gene: 73541128

Following gene: 73541130

Centisome position: 40.73

GC content: 67.26

Gene sequence:

>1014_bases
ATGCTCTATCCCTGGCAAACGGAAGACTGGCAACGGCTTGGCGCGCTGCGCGAGCGGCTCCCCCATGCATTGCTGATCCA
TGGCCAGCAGGGCACCGGCAAGCGTGACCTGGCGCTGCACTTTGCGCAGGGTCTGCTGTGCGAATCGCCGCGGCCAGACG
GCCAGCCCTGTGGCCAGTGCGCTGCCTGTCACTGGTTCAGCCAGGGCAACCATCCTGATTTCACGGTGGTACGACCTGAA
GCGCTGGAGTCCGGCGGTGAAGGCGAGGCCGATGACAGCGGCAAGAAGAAGGCGCCCAGCAAGATCATTCGCATGGAGCA
GGTGCGCGCGCTGATTGACGCGGTGGGGGTGGGCACGCACCGGGCGGGCCTGCGCGTTGTGGTGGTCTATCCGCTGGACG
CGCTGCAGGTTGAAGGGGCCAACGCGTTGCTGAAGACGCTCGAGGAGCCCCCGTCGTCGACGGTCTTCCTGCTCGTTACG
GACCGGCTCGATCGCATCCTGCCGACCATCCTGTCGCGCTGCCGCCAGTTTTCCACTCAGCGGCCAACGCCAGAAGCGGC
GCTGGCCTGGCTCAAGAGCCAGGGCGTTGCCGACGCCGAGGCGCAGCTTGCGCTGGCTGGCGGCTCGCCGCTGACCGCTC
TGCATGCCGCGGAGGCCGAGGAGCAGCCGCTGCAGCGCTGGCTGGTCGGCCAACTGGGTGCTGGCGCCGAATTTGACGCG
ACTGGTGCGGCGGAACAACTACAAAAGCTGCCGGTGCCCGCCGTGCTTGGCATCCTCCAGCGCTGGACGTACGATCTGCT
GAAGTGCCGGCTCGACGCCTCGGGCAACGCGGTGCCGCGCTACTTCCCGAAGGAGCGCACCGTGCTGGCGCGTTGCGCGG
GTGCCTCCAATGCCCATCGGCTGCAGGCATTTGCCACGCGCCTCAGCGCGCACCGGCGCAGCGAGAACCATCCGCTCGCG
CCGAGGCTGGTGATGGAATCCGTGTTTCTTGAATACCGGCAGCTGTTCCACTGA

Upstream 100 bases:

>100_bases
TGATCGATAATAATGGACCGACGCTGTGCCGTTACGTCATCGGCAGCGGCACGGTTGTAAAGGTGACCCCTCGGCTTTCG
CGGCTAACTGACTGATTCGC

Downstream 100 bases:

>100_bases
CGCCTCCGGCGCAGTACGCGGGGCCCGGCTACAGCCGGGGTGCAGGACCAAAACATCTATAACACAAAGCTGTAGCAGGT
CAGAAAGGGGTCAGTCATGA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MLYPWQTEDWQRLGALRERLPHALLIHGQQGTGKRDLALHFAQGLLCESPRPDGQPCGQCAACHWFSQGNHPDFTVVRPE
ALESGGEGEADDSGKKKAPSKIIRMEQVRALIDAVGVGTHRAGLRVVVVYPLDALQVEGANALLKTLEEPPSSTVFLLVT
DRLDRILPTILSRCRQFSTQRPTPEAALAWLKSQGVADAEAQLALAGGSPLTALHAAEAEEQPLQRWLVGQLGAGAEFDA
TGAAEQLQKLPVPAVLGILQRWTYDLLKCRLDASGNAVPRYFPKERTVLARCAGASNAHRLQAFATRLSAHRRSENHPLA
PRLVMESVFLEYRQLFH

Sequences:

>Translated_337_residues
MLYPWQTEDWQRLGALRERLPHALLIHGQQGTGKRDLALHFAQGLLCESPRPDGQPCGQCAACHWFSQGNHPDFTVVRPE
ALESGGEGEADDSGKKKAPSKIIRMEQVRALIDAVGVGTHRAGLRVVVVYPLDALQVEGANALLKTLEEPPSSTVFLLVT
DRLDRILPTILSRCRQFSTQRPTPEAALAWLKSQGVADAEAQLALAGGSPLTALHAAEAEEQPLQRWLVGQLGAGAEFDA
TGAAEQLQKLPVPAVLGILQRWTYDLLKCRLDASGNAVPRYFPKERTVLARCAGASNAHRLQAFATRLSAHRRSENHPLA
PRLVMESVFLEYRQLFH
>Mature_337_residues
MLYPWQTEDWQRLGALRERLPHALLIHGQQGTGKRDLALHFAQGLLCESPRPDGQPCGQCAACHWFSQGNHPDFTVVRPE
ALESGGEGEADDSGKKKAPSKIIRMEQVRALIDAVGVGTHRAGLRVVVVYPLDALQVEGANALLKTLEEPPSSTVFLLVT
DRLDRILPTILSRCRQFSTQRPTPEAALAWLKSQGVADAEAQLALAGGSPLTALHAAEAEEQPLQRWLVGQLGAGAEFDA
TGAAEQLQKLPVPAVLGILQRWTYDLLKCRLDASGNAVPRYFPKERTVLARCAGASNAHRLQAFATRLSAHRRSENHPLA
PRLVMESVFLEYRQLFH

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=194, Percent_Identity=33.5051546391753, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786676, Length=159, Percent_Identity=35.2201257861635, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36954; Mature: 36954

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYPWQTEDWQRLGALRERLPHALLIHGQQGTGKRDLALHFAQGLLCESPRPDGQPCGQC
CCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHH
AACHWFSQGNHPDFTVVRPEALESGGEGEADDSGKKKAPSKIIRMEQVRALIDAVGVGTH
HHHHHHCCCCCCCEEEECHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
RAGLRVVVVYPLDALQVEGANALLKTLEEPPSSTVFLLVTDRLDRILPTILSRCRQFSTQ
CCCCEEEEEECCCCEEECCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCC
RPTPEAALAWLKSQGVADAEAQLALAGGSPLTALHAAEAEEQPLQRWLVGQLGAGAEFDA
CCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCC
TGAAEQLQKLPVPAVLGILQRWTYDLLKCRLDASGNAVPRYFPKERTVLARCAGASNAHR
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHH
LQAFATRLSAHRRSENHPLAPRLVMESVFLEYRQLFH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLYPWQTEDWQRLGALRERLPHALLIHGQQGTGKRDLALHFAQGLLCESPRPDGQPCGQC
CCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHH
AACHWFSQGNHPDFTVVRPEALESGGEGEADDSGKKKAPSKIIRMEQVRALIDAVGVGTH
HHHHHHCCCCCCCEEEECHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
RAGLRVVVVYPLDALQVEGANALLKTLEEPPSSTVFLLVTDRLDRILPTILSRCRQFSTQ
CCCCEEEEEECCCCEEECCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCC
RPTPEAALAWLKSQGVADAEAQLALAGGSPLTALHAAEAEEQPLQRWLVGQLGAGAEFDA
CCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCC
TGAAEQLQKLPVPAVLGILQRWTYDLLKCRLDASGNAVPRYFPKERTVLARCAGASNAHR
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHH
LQAFATRLSAHRRSENHPLAPRLVMESVFLEYRQLFH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]