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The map label for this gene is fadA [H]

Identifier: 73541101

GI number: 73541101

Start: 1522936

End: 1524114

Strand: Direct

Name: fadA [H]

Synonym: Reut_A1407

Alternate gene names: 73541101

Gene position: 1522936-1524114 (Clockwise)

Preceding gene: 73541100

Following gene: 73541102

Centisome position: 40.01

GC content: 66.07

Gene sequence:

>1179_bases
ATGAACGAGGCAGTCATCGTATCCACCGCCCGCACCGGGCTGGCCAAGAGCTGGAAGGGCGCGTTCAACATGACCCATGG
CGCGACGCTCGGCGGCCACGCCGTGGAGCACGCCATTGCGCGCGCAAAGATCGAAGCCGGCGAAGTGGAAGACGTGCTGA
TGGGCTGTGCCAACCCCGAAGGCGCCACCGGCTCCAACATCGCCCGCCAGATCGCGCTGCGTGCTGGTTGCCCGGTCACC
GTGCCGGGTGCGACCGTGAACCGCTTCTGCTCGTCCGGCCTGCAGACCATTGCTATGGCGGCGCAGCGCGTGATCGCCGA
TGAAGGCGATATCTTCGTGGCCGGCGGCGTGGAAAGCATCTCCTGCGTCCAGGCGGAAATGAACCGCCACATGCTCGCCG
ATCCGTGGCTGGTCAAGAACAAGCCGGAAATCTACTGGAACATGCTCCAGACCGCCGAGAACGTGGCCAAGCGCTACAAC
ATCTCGAAGGATCGCCAGGACGAGTATGGCGTGCGCAGCCAGCAGCGCGCCGCCGCCGCCCAGGAAGCCGGCAAGTTCAA
GGACGAGATCGTTCCGATCACGGTGCTCGCAGGCGTGGCCGACAAGTCCACGGGCCAGTTGGTCACGAAGGAAGTGACGC
TGGACCGCGACGAAGGCATCCGTGGCGACACCACGCTGGAAGCCGTGTCGAAAATCCGTACTGCCGTGCCGGGTGGCGTG
GTCACCGCCGGCAATGCGTCGCAGTTCTCGGACGGCGCTTCGGCTGCCGTGGTGATGAACGCGCGCGTCGCTGCCGCCAA
GGGCCTGCAGCCGCTGGGCGTGTTCCGCGGCTTCGCCGTGGCGGGTTGCGAGCCCGATGAAATGGGCATCGGCCCGGTGT
TCGCGGTGCCCAAGCTGCTGAAGAAGGCCGGCCTGAAGGTCGACGACATCGGCCTGTGGGAACTGAACGAAGCCTTCGCC
GTGCAGGTGCTGTACTGCGCCGACAAGCTGGGCATCCCGATGGATCGCCTGAACGTCAACGGTGGCGCTATCGCGGTGGG
CCACCCGTATGGCGTGTCGGGTGCTCGCCTGGTCGGCCATGCGCTGATTGAAGGCAAGCGCCGTGGCGTGAAGTACGTCG
TCGTGACGATGTGCATCGGCGGCGGGCAGGGCGCGGCCGGGTTGTTCGAAGTGCTGTAA

Upstream 100 bases:

>100_bases
CCGCTGGCGTCAAGCGCATCCACGCGGCCGAACTGCTGCAAGCCCCGGGCTGAACCGAACTAACCGAACTGAAAGACACC
AAGATTCCGAGGAGCAAGAC

Downstream 100 bases:

>100_bases
GTCTGGTTTGCTTCCCTCTCCCGTGCGCGGGAGAGGGAGCACCCATGTAGTCACTGAAAGGCAGGCGTTCCTGCAAATTC
AATACCGCTCCAGGGTCATT

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MNEAVIVSTARTGLAKSWKGAFNMTHGATLGGHAVEHAIARAKIEAGEVEDVLMGCANPEGATGSNIARQIALRAGCPVT
VPGATVNRFCSSGLQTIAMAAQRVIADEGDIFVAGGVESISCVQAEMNRHMLADPWLVKNKPEIYWNMLQTAENVAKRYN
ISKDRQDEYGVRSQQRAAAAQEAGKFKDEIVPITVLAGVADKSTGQLVTKEVTLDRDEGIRGDTTLEAVSKIRTAVPGGV
VTAGNASQFSDGASAAVVMNARVAAAKGLQPLGVFRGFAVAGCEPDEMGIGPVFAVPKLLKKAGLKVDDIGLWELNEAFA
VQVLYCADKLGIPMDRLNVNGGAIAVGHPYGVSGARLVGHALIEGKRRGVKYVVVTMCIGGGQGAAGLFEVL

Sequences:

>Translated_392_residues
MNEAVIVSTARTGLAKSWKGAFNMTHGATLGGHAVEHAIARAKIEAGEVEDVLMGCANPEGATGSNIARQIALRAGCPVT
VPGATVNRFCSSGLQTIAMAAQRVIADEGDIFVAGGVESISCVQAEMNRHMLADPWLVKNKPEIYWNMLQTAENVAKRYN
ISKDRQDEYGVRSQQRAAAAQEAGKFKDEIVPITVLAGVADKSTGQLVTKEVTLDRDEGIRGDTTLEAVSKIRTAVPGGV
VTAGNASQFSDGASAAVVMNARVAAAKGLQPLGVFRGFAVAGCEPDEMGIGPVFAVPKLLKKAGLKVDDIGLWELNEAFA
VQVLYCADKLGIPMDRLNVNGGAIAVGHPYGVSGARLVGHALIEGKRRGVKYVVVTMCIGGGQGAAGLFEVL
>Mature_392_residues
MNEAVIVSTARTGLAKSWKGAFNMTHGATLGGHAVEHAIARAKIEAGEVEDVLMGCANPEGATGSNIARQIALRAGCPVT
VPGATVNRFCSSGLQTIAMAAQRVIADEGDIFVAGGVESISCVQAEMNRHMLADPWLVKNKPEIYWNMLQTAENVAKRYN
ISKDRQDEYGVRSQQRAAAAQEAGKFKDEIVPITVLAGVADKSTGQLVTKEVTLDRDEGIRGDTTLEAVSKIRTAVPGGV
VTAGNASQFSDGASAAVVMNARVAAAKGLQPLGVFRGFAVAGCEPDEMGIGPVFAVPKLLKKAGLKVDDIGLWELNEAFA
VQVLYCADKLGIPMDRLNVNGGAIAVGHPYGVSGARLVGHALIEGKRRGVKYVVVTMCIGGGQGAAGLFEVL

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=391, Percent_Identity=41.6879795396419, Blast_Score=258, Evalue=7e-69,
Organism=Homo sapiens, GI4557237, Length=405, Percent_Identity=38.5185185185185, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI167614485, Length=397, Percent_Identity=36.7758186397985, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=35.1758793969849, Blast_Score=204, Evalue=9e-53,
Organism=Homo sapiens, GI4504327, Length=401, Percent_Identity=31.4214463840399, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI194353979, Length=394, Percent_Identity=30.7106598984772, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI1787663, Length=412, Percent_Identity=40.0485436893204, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI48994986, Length=396, Percent_Identity=40.4040404040404, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI1788554, Length=416, Percent_Identity=39.9038461538462, Blast_Score=241, Evalue=8e-65,
Organism=Escherichia coli, GI87082165, Length=402, Percent_Identity=37.3134328358209, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1788683, Length=421, Percent_Identity=30.6413301662708, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI133906874, Length=397, Percent_Identity=39.2947103274559, Blast_Score=244, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI17535921, Length=403, Percent_Identity=34.9875930521092, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17551802, Length=437, Percent_Identity=31.5789473684211, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI25147385, Length=401, Percent_Identity=31.9201995012469, Blast_Score=184, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI17535917, Length=391, Percent_Identity=26.3427109974425, Blast_Score=128, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6322031, Length=396, Percent_Identity=38.1313131313131, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6325229, Length=400, Percent_Identity=33, Blast_Score=177, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24655093, Length=403, Percent_Identity=39.2059553349876, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24640423, Length=407, Percent_Identity=35.8722358722359, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI17648125, Length=359, Percent_Identity=38.7186629526462, Blast_Score=222, Evalue=3e-58,
Organism=Drosophila melanogaster, GI17137578, Length=410, Percent_Identity=33.6585365853659, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 40953; Mature: 40953

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEAVIVSTARTGLAKSWKGAFNMTHGATLGGHAVEHAIARAKIEAGEVEDVLMGCANPE
CCCEEEEEECCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCC
GATGSNIARQIALRAGCPVTVPGATVNRFCSSGLQTIAMAAQRVIADEGDIFVAGGVESI
CCCCHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHH
SCVQAEMNRHMLADPWLVKNKPEIYWNMLQTAENVAKRYNISKDRQDEYGVRSQQRAAAA
HHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHH
QEAGKFKDEIVPITVLAGVADKSTGQLVTKEVTLDRDEGIRGDTTLEAVSKIRTAVPGGV
HHHCCHHHCEEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCE
VTAGNASQFSDGASAAVVMNARVAAAKGLQPLGVFRGFAVAGCEPDEMGIGPVFAVPKLL
EECCCCHHCCCCCCEEEEEECHHHHHCCCCHHHHHCCCEEECCCCCCCCCCHHHHHHHHH
KKAGLKVDDIGLWELNEAFAVQVLYCADKLGIPMDRLNVNGGAIAVGHPYGVSGARLVGH
HHCCCCEECCCCEECCHHHEEEEEEEHHHHCCCHHHEECCCCEEEECCCCCCCHHHHHHH
ALIEGKRRGVKYVVVTMCIGGGQGAAGLFEVL
HHHHHCCCCCEEEEEEEEECCCCCCCHHHHCC
>Mature Secondary Structure
MNEAVIVSTARTGLAKSWKGAFNMTHGATLGGHAVEHAIARAKIEAGEVEDVLMGCANPE
CCCEEEEEECCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCC
GATGSNIARQIALRAGCPVTVPGATVNRFCSSGLQTIAMAAQRVIADEGDIFVAGGVESI
CCCCHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHH
SCVQAEMNRHMLADPWLVKNKPEIYWNMLQTAENVAKRYNISKDRQDEYGVRSQQRAAAA
HHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHH
QEAGKFKDEIVPITVLAGVADKSTGQLVTKEVTLDRDEGIRGDTTLEAVSKIRTAVPGGV
HHHCCHHHCEEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCE
VTAGNASQFSDGASAAVVMNARVAAAKGLQPLGVFRGFAVAGCEPDEMGIGPVFAVPKLL
EECCCCHHCCCCCCEEEEEECHHHHHCCCCHHHHHCCCEEECCCCCCCCCCHHHHHHHHH
KKAGLKVDDIGLWELNEAFAVQVLYCADKLGIPMDRLNVNGGAIAVGHPYGVSGARLVGH
HHCCCCEECCCCEECCHHHEEEEEEEHHHHCCCHHHEECCCCEEEECCCCCCCHHHHHHH
ALIEGKRRGVKYVVVTMCIGGGQGAAGLFEVL
HHHHHCCCCCEEEEEEEEECCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]