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The map label for this gene is pncA [H]

Identifier: 73541100

GI number: 73541100

Start: 1522250

End: 1522888

Strand: Direct

Name: pncA [H]

Synonym: Reut_A1406

Alternate gene names: 73541100

Gene position: 1522250-1522888 (Clockwise)

Preceding gene: 73541099

Following gene: 73541101

Centisome position: 39.99

GC content: 68.39

Gene sequence:

>639_bases
ATGAGACCCGGTCCGGATGACTGCCTGCTGGTAGTCGACGTGCAGAACGACTTCATGCCAGGCGGCGCGCTTGCCGTGCC
GCGTGGCGATGAGATCGTGCCTGTCGTCAACCGGCTCGCGCATGCATTCCCGCACGTGGTGCTGACGCAGGACTGGCACC
CGGCGGACCATGTCTCGTTTGCCGCCAACCATGCCGGTACGGAGCCGTTCCAGATGCTCGCGTTGCCGTATGGCCAGCAG
GTGCTGTGGCCGGTCCATTGCGTGCAGGGCACAACGGGTGCGGCGTTCCACGCCGGGCTCGACGTGCCCCACGCCCGGCT
GGTGATCCGAAAGGGCCATCACACGGGCGTGGACAGCTACTCCGCCTTCATGGAGGCGGACCGCAGCACGCGCACCGGAC
TGGCCGGCTACCTGCGAGAGCACGGCGTGAAGCGCGTGTTCCTGGCCGGACTGGCGACGGACTACTGCGTGGCGTGGAGC
GCGCTCGATGCGCGCGCTGCAGGCTTCGACGCCGTGTTGATCGAGGATGCGTGCCGTGCCATCGACCTTAACGGGTCGCT
GGCGCAGGCGTGGCAGGACCTCGCAGCCGCTGGCGTCAAGCGCATCCACGCGGCCGAACTGCTGCAAGCCCCGGGCTGA

Upstream 100 bases:

>100_bases
GAAGCGTGGCAAGTCGCGCCGCTGCTGCAGAAGCTGGCGGACGAGGGCAAGGGCTTCAACGACTGAAGCCAAGGCAGCAG
CAAGGCTGACGGAGGCGAAC

Downstream 100 bases:

>100_bases
ACCGAACTAACCGAACTGAAAGACACCAAGATTCCGAGGAGCAAGACATGAACGAGGCAGTCATCGTATCCACCGCCCGC
ACCGGGCTGGCCAAGAGCTG

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MRPGPDDCLLVVDVQNDFMPGGALAVPRGDEIVPVVNRLAHAFPHVVLTQDWHPADHVSFAANHAGTEPFQMLALPYGQQ
VLWPVHCVQGTTGAAFHAGLDVPHARLVIRKGHHTGVDSYSAFMEADRSTRTGLAGYLREHGVKRVFLAGLATDYCVAWS
ALDARAAGFDAVLIEDACRAIDLNGSLAQAWQDLAAAGVKRIHAAELLQAPG

Sequences:

>Translated_212_residues
MRPGPDDCLLVVDVQNDFMPGGALAVPRGDEIVPVVNRLAHAFPHVVLTQDWHPADHVSFAANHAGTEPFQMLALPYGQQ
VLWPVHCVQGTTGAAFHAGLDVPHARLVIRKGHHTGVDSYSAFMEADRSTRTGLAGYLREHGVKRVFLAGLATDYCVAWS
ALDARAAGFDAVLIEDACRAIDLNGSLAQAWQDLAAAGVKRIHAAELLQAPG
>Mature_212_residues
MRPGPDDCLLVVDVQNDFMPGGALAVPRGDEIVPVVNRLAHAFPHVVLTQDWHPADHVSFAANHAGTEPFQMLALPYGQQ
VLWPVHCVQGTTGAAFHAGLDVPHARLVIRKGHHTGVDSYSAFMEADRSTRTGLAGYLREHGVKRVFLAGLATDYCVAWS
ALDARAAGFDAVLIEDACRAIDLNGSLAQAWQDLAAAGVKRIHAAELLQAPG

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=216, Percent_Identity=42.1296296296296, Blast_Score=155, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI193206735, Length=222, Percent_Identity=31.0810810810811, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17543130, Length=222, Percent_Identity=31.0810810810811, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71999339, Length=202, Percent_Identity=33.1683168316832, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6321401, Length=191, Percent_Identity=34.0314136125654, Blast_Score=116, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648247, Length=202, Percent_Identity=36.1386138613861, Blast_Score=120, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22682; Mature: 22682

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPGPDDCLLVVDVQNDFMPGGALAVPRGDEIVPVVNRLAHAFPHVVLTQDWHPADHVSF
CCCCCCCEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHH
AANHAGTEPFQMLALPYGQQVLWPVHCVQGTTGAAFHAGLDVPHARLVIRKGHHTGVDSY
HHCCCCCCCCEEEECCCCCCEEEEEHHHCCCCCCEEECCCCCCCCEEEEECCCCCCCHHH
SAFMEADRSTRTGLAGYLREHGVKRVFLAGLATDYCVAWSALDARAAGFDAVLIEDACRA
HHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHE
IDLNGSLAQAWQDLAAAGVKRIHAAELLQAPG
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRPGPDDCLLVVDVQNDFMPGGALAVPRGDEIVPVVNRLAHAFPHVVLTQDWHPADHVSF
CCCCCCCEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHH
AANHAGTEPFQMLALPYGQQVLWPVHCVQGTTGAAFHAGLDVPHARLVIRKGHHTGVDSY
HHCCCCCCCCEEEECCCCCCEEEEEHHHCCCCCCEEECCCCCCCCEEEEECCCCCCCHHH
SAFMEADRSTRTGLAGYLREHGVKRVFLAGLATDYCVAWSALDARAAGFDAVLIEDACRA
HHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHE
IDLNGSLAQAWQDLAAAGVKRIHAAELLQAPG
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]