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The map label for this gene is ispF

Identifier: 73541056

GI number: 73541056

Start: 1466775

End: 1467284

Strand: Direct

Name: ispF

Synonym: Reut_A1362

Alternate gene names: 73541056

Gene position: 1466775-1467284 (Clockwise)

Preceding gene: 73541055

Following gene: 73541063

Centisome position: 38.53

GC content: 65.49

Gene sequence:

>510_bases
ATGATGCCTTTTGATATCCGCGTGGGGCAGGGCTATGACGTCCACGCACTTGTGCCCGGCCGCAAGCTGATCCTCGGCGG
CGTGCATATTCCACACGACCGCGGCCTGCTCGGGCATTCCGATGCCGATGCGCTGCTCCATGCCATTACGGACGCGCTGT
TCGGCGCGGCAGGGCTTGGCGACATCGGGCGCCATTTCCCGGACACGGATGCACAGTTTGCCGGTGCCGACAGCCGCGTG
CTGCTGCGTGAGGCCGCGCGCCGCGTGCGCGAGGCCGGCTACGAAATCGGCAATGTCGATGCGTCGGTGATCGCGCAGCA
GCCCAAGCTGGCACCGCATATTCCGGGCATGGTTGCGAATATTGCCGATGACCTGGGCCTGCCCGCCACGCGTTGCAACG
TCAAGGCCAAGACCAACGAAAAGCTTGGCTTCGAAGGACGGCAGGAAGGTATCGTGGCGCAGGCCGCGGTCCTGATCTGG
CGCGGCGCAATCGCGGACGCGCAGGACTGA

Upstream 100 bases:

>100_bases
GCTGCGCAATTTCAAAGTGACATATCCGGAAGACTTCGCGCTGGCCGAAGTGCTGCTCGGCACCGGGCCGGCCAGGTCTG
CCGATTCAGGAGCCTGATCA

Downstream 100 bases:

>100_bases
GCGCGGCCCGGATCCCTGGCCGAAGCGGAAGCGAAAGCGCTTACGCCTTGGCCGGTGCAGGCGCGCTCTCTGGCGTGATG
GCGTAGCTGTAGTCGATCAG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MMPFDIRVGQGYDVHALVPGRKLILGGVHIPHDRGLLGHSDADALLHAITDALFGAAGLGDIGRHFPDTDAQFAGADSRV
LLREAARRVREAGYEIGNVDASVIAQQPKLAPHIPGMVANIADDLGLPATRCNVKAKTNEKLGFEGRQEGIVAQAAVLIW
RGAIADAQD

Sequences:

>Translated_169_residues
MMPFDIRVGQGYDVHALVPGRKLILGGVHIPHDRGLLGHSDADALLHAITDALFGAAGLGDIGRHFPDTDAQFAGADSRV
LLREAARRVREAGYEIGNVDASVIAQQPKLAPHIPGMVANIADDLGLPATRCNVKAKTNEKLGFEGRQEGIVAQAAVLIW
RGAIADAQD
>Mature_169_residues
MMPFDIRVGQGYDVHALVPGRKLILGGVHIPHDRGLLGHSDADALLHAITDALFGAAGLGDIGRHFPDTDAQFAGADSRV
LLREAARRVREAGYEIGNVDASVIAQQPKLAPHIPGMVANIADDLGLPATRCNVKAKTNEKLGFEGRQEGIVAQAAVLIW
RGAIADAQD

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=58.3333333333333, Blast_Score=176, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_CUPPJ (Q472F1)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_295576.1
- ProteinModelPortal:   Q472F1
- SMR:   Q472F1
- GeneID:   3611561
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A1362
- NMPDR:   fig|264198.3.peg.2103
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   REUT264198:REUT_A1362-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17883; Mature: 17883

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPFDIRVGQGYDVHALVPGRKLILGGVHIPHDRGLLGHSDADALLHAITDALFGAAGLG
CCCCEEEECCCCCEEEECCCCEEEECCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
DIGRHFPDTDAQFAGADSRVLLREAARRVREAGYEIGNVDASVIAQQPKLAPHIPGMVAN
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHH
IADDLGLPATRCNVKAKTNEKLGFEGRQEGIVAQAAVLIWRGAIADAQD
HHHHHCCCCHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MMPFDIRVGQGYDVHALVPGRKLILGGVHIPHDRGLLGHSDADALLHAITDALFGAAGLG
CCCCEEEECCCCCEEEECCCCEEEECCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
DIGRHFPDTDAQFAGADSRVLLREAARRVREAGYEIGNVDASVIAQQPKLAPHIPGMVAN
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHH
IADDLGLPATRCNVKAKTNEKLGFEGRQEGIVAQAAVLIWRGAIADAQD
HHHHHCCCCHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA