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The map label for this gene is thlA [H]

Identifier: 73541047

GI number: 73541047

Start: 1455089

End: 1456273

Strand: Direct

Name: thlA [H]

Synonym: Reut_A1353

Alternate gene names: 73541047

Gene position: 1455089-1456273 (Clockwise)

Preceding gene: 73541046

Following gene: 73541048

Centisome position: 38.23

GC content: 66.33

Gene sequence:

>1185_bases
ATGACGCGTGAAGTCGTGGTAGTGAGTGGCGTACGTACGGCAATCGGCACTTTCGGCGGCAGCCTGAAGGACATGGCACC
GACCCAACTGGGTGCACTCGTGGTGCGCGAGGCGCTGGCCCGTGCTCAGGTGTCGGGCGACGATGTTGGTCACGTGGTGT
TCGGTAACGTGATTCAGACCGAGCCGCGCGACATGTATCTGGGCCGCGTGGCGGCCGTCGATGGCGGTGTGACGATCAAT
GCGCCTGCGCTGACCGTGAACCGCCTGTGCGGCTCGGGCCTGCAAGCCATCGTCAGCGCCGCGCAGACCATTCTGCTCGG
CGACGCCGACGTGGCCATCGGCGGCGGTGCGGAAAGCATGAGCCGCGCGCCGTACCTGGCGCCCGCAGTTCGCTGGGGCG
CGCGCATGGGCGATGCCAGCATGGTCGACATGATGCTGGGCGCGCTGCATGACCCGTTCCACCGCATTCACATGGGTGTC
ACGGCCGAGAACGTGGCCAAGGAATACGACATCACGCGCAGCCAGCAGGACGAAGCCGCGCTGGAATCGCACCGTCGCGC
TTCGGCCGCGATCAAGGCTGGTTACTTCAAGGACCAGATCGTTCCGGTGACGCTCAAGACCCGCAAGGGCGAGGTGAGCT
TCGACACCGACGAGCACGTGCGTCACGACGCCACGATCGACGACATGACCAAGCTCAAGCCGGTCTTCGTCAAGGAAAAC
GGCACCGTGACGGCGGGCAATGCCTCCGGCCTGAACGACGCCGCTGCGGCTGTCGTGCTGATGGAGCGCGCCGAAGCCGA
GCGCCGTGACCTGAAGCCGCTGGCTCGCCTGGTGTCGTATGGCCACGCTGGCGTGGATCCGAAGACCATGGGTATCGGCC
CGGTGCCGGCTACCAAGAAGGCGCTGGAGCGCGCTGGCCTGTCGGTGTCCGACCTGGACGTGATCGAAGCCAACGAAGCG
TTTGCCGCGCAGGCTTGCGCGGTGACCAAGGCGCTGGGCCTGGACCCGGCCAAGGTCAATCCCAACGGTTCCGGCATTTC
GCTTGGCCACCCGATCGGCGCCACCGGCGCGCTGATCACGGTCAAGGCGTTGCATGAGCTGCAACGCGTGCAGGGCCGCT
ATGCGCTGGTGACGATGTGCATCGGCGGCGGCCAGGGCATTGCCGCCATCTTCGAGCGCATCTGA

Upstream 100 bases:

>100_bases
TGCAACGGGCTTCTGCTGCCCTTAAGTAAAAGTACGATCGTTCGATTCCGTCGCGATGCCGCTATACTGTGCACCGCAAC
ATAACCTCATGGAGAGAGAC

Downstream 100 bases:

>100_bases
GTCTTGTTGATGTGTCGTCGCACCCCGTTGCAGCGCCGTTCGCTCGTCAGCCTGGCTGCCGCGCTGGCGCTTGCGGGGGC
GATGCCGGTGCGGGCCGCCG

Product: beta-ketothiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MTREVVVVSGVRTAIGTFGGSLKDMAPTQLGALVVREALARAQVSGDDVGHVVFGNVIQTEPRDMYLGRVAAVDGGVTIN
APALTVNRLCGSGLQAIVSAAQTILLGDADVAIGGGAESMSRAPYLAPAVRWGARMGDASMVDMMLGALHDPFHRIHMGV
TAENVAKEYDITRSQQDEAALESHRRASAAIKAGYFKDQIVPVTLKTRKGEVSFDTDEHVRHDATIDDMTKLKPVFVKEN
GTVTAGNASGLNDAAAAVVLMERAEAERRDLKPLARLVSYGHAGVDPKTMGIGPVPATKKALERAGLSVSDLDVIEANEA
FAAQACAVTKALGLDPAKVNPNGSGISLGHPIGATGALITVKALHELQRVQGRYALVTMCIGGGQGIAAIFERI

Sequences:

>Translated_394_residues
MTREVVVVSGVRTAIGTFGGSLKDMAPTQLGALVVREALARAQVSGDDVGHVVFGNVIQTEPRDMYLGRVAAVDGGVTIN
APALTVNRLCGSGLQAIVSAAQTILLGDADVAIGGGAESMSRAPYLAPAVRWGARMGDASMVDMMLGALHDPFHRIHMGV
TAENVAKEYDITRSQQDEAALESHRRASAAIKAGYFKDQIVPVTLKTRKGEVSFDTDEHVRHDATIDDMTKLKPVFVKEN
GTVTAGNASGLNDAAAAVVLMERAEAERRDLKPLARLVSYGHAGVDPKTMGIGPVPATKKALERAGLSVSDLDVIEANEA
FAAQACAVTKALGLDPAKVNPNGSGISLGHPIGATGALITVKALHELQRVQGRYALVTMCIGGGQGIAAIFERI
>Mature_393_residues
TREVVVVSGVRTAIGTFGGSLKDMAPTQLGALVVREALARAQVSGDDVGHVVFGNVIQTEPRDMYLGRVAAVDGGVTINA
PALTVNRLCGSGLQAIVSAAQTILLGDADVAIGGGAESMSRAPYLAPAVRWGARMGDASMVDMMLGALHDPFHRIHMGVT
AENVAKEYDITRSQQDEAALESHRRASAAIKAGYFKDQIVPVTLKTRKGEVSFDTDEHVRHDATIDDMTKLKPVFVKENG
TVTAGNASGLNDAAAAVVLMERAEAERRDLKPLARLVSYGHAGVDPKTMGIGPVPATKKALERAGLSVSDLDVIEANEAF
AAQACAVTKALGLDPAKVNPNGSGISLGHPIGATGALITVKALHELQRVQGRYALVTMCIGGGQGIAAIFERI

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=391, Percent_Identity=47.3145780051151, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI167614485, Length=393, Percent_Identity=45.8015267175573, Blast_Score=355, Evalue=3e-98,
Organism=Homo sapiens, GI4557237, Length=400, Percent_Identity=41, Blast_Score=281, Evalue=8e-76,
Organism=Homo sapiens, GI4501853, Length=396, Percent_Identity=41.1616161616162, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=35.7647058823529, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI194353979, Length=391, Percent_Identity=31.2020460358056, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1788554, Length=398, Percent_Identity=50.251256281407, Blast_Score=347, Evalue=7e-97,
Organism=Escherichia coli, GI87082165, Length=392, Percent_Identity=46.1734693877551, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=45.3865336658354, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI48994986, Length=399, Percent_Identity=40.1002506265664, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1788683, Length=412, Percent_Identity=33.252427184466, Blast_Score=204, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI133906874, Length=391, Percent_Identity=46.8030690537084, Blast_Score=360, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI25147385, Length=394, Percent_Identity=36.8020304568528, Blast_Score=252, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17535921, Length=396, Percent_Identity=33.8383838383838, Blast_Score=218, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17551802, Length=424, Percent_Identity=31.6037735849057, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17535917, Length=406, Percent_Identity=28.0788177339901, Blast_Score=157, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6325229, Length=406, Percent_Identity=39.1625615763547, Blast_Score=273, Evalue=5e-74,
Organism=Saccharomyces cerevisiae, GI6322031, Length=399, Percent_Identity=36.5914786967419, Blast_Score=223, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=45.4081632653061, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI17648125, Length=395, Percent_Identity=45.3164556962025, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24640423, Length=397, Percent_Identity=41.0579345088161, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=33.0210772833724, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41145; Mature: 41013

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTREVVVVSGVRTAIGTFGGSLKDMAPTQLGALVVREALARAQVSGDDVGHVVFGNVIQT
CCCCEEEECCHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCC
EPRDMYLGRVAAVDGGVTINAPALTVNRLCGSGLQAIVSAAQTILLGDADVAIGGGAESM
CCHHHHHEEEEEECCCEEECCCHHHHHHHHCCHHHHHHHHHHHEEECCCCEEECCCHHHH
SRAPYLAPAVRWGARMGDASMVDMMLGALHDPFHRIHMGVTAENVAKEYDITRSQQDEAA
CCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHCCCCCCCHHHH
LESHRRASAAIKAGYFKDQIVPVTLKTRKGEVSFDTDEHVRHDATIDDMTKLKPVFVKEN
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHCCCCHHHHHHCCEEEEECC
GTVTAGNASGLNDAAAAVVLMERAEAERRDLKPLARLVSYGHAGVDPKTMGIGPVPATKK
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
ALERAGLSVSDLDVIEANEAFAAQACAVTKALGLDPAKVNPNGSGISLGHPIGATGALIT
HHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEECCCCCCEECCCCCCCCCHHHH
VKALHELQRVQGRYALVTMCIGGGQGIAAIFERI
HHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure 
TREVVVVSGVRTAIGTFGGSLKDMAPTQLGALVVREALARAQVSGDDVGHVVFGNVIQT
CCCEEEECCHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCC
EPRDMYLGRVAAVDGGVTINAPALTVNRLCGSGLQAIVSAAQTILLGDADVAIGGGAESM
CCHHHHHEEEEEECCCEEECCCHHHHHHHHCCHHHHHHHHHHHEEECCCCEEECCCHHHH
SRAPYLAPAVRWGARMGDASMVDMMLGALHDPFHRIHMGVTAENVAKEYDITRSQQDEAA
CCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHCCCCCCCHHHH
LESHRRASAAIKAGYFKDQIVPVTLKTRKGEVSFDTDEHVRHDATIDDMTKLKPVFVKEN
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHCCCCHHHHHHCCEEEEECC
GTVTAGNASGLNDAAAAVVLMERAEAERRDLKPLARLVSYGHAGVDPKTMGIGPVPATKK
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
ALERAGLSVSDLDVIEANEAFAAQACAVTKALGLDPAKVNPNGSGISLGHPIGATGALIT
HHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEECCCCCCEECCCCCCCCCHHHH
VKALHELQRVQGRYALVTMCIGGGQGIAAIFERI
HHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]