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The map label for this gene is rimO [H]

Identifier: 73541046

GI number: 73541046

Start: 1453630

End: 1454988

Strand: Direct

Name: rimO [H]

Synonym: Reut_A1352

Alternate gene names: 73541046

Gene position: 1453630-1454988 (Clockwise)

Preceding gene: 73541045

Following gene: 73541047

Centisome position: 38.19

GC content: 65.34

Gene sequence:

>1359_bases
ATGACCCAGAAAGACCAGAGCCCGCGCGTCGGCTTCGTTTCGCTTGGCTGCCCGAAGGCGTTGGTCGACTCGGAACAGAT
CATCACCCAGCTGCGCGCCGAGGGTTACGCAATCAGCGGCACCTATGACGGTGCCGACCTGGTTGTGGTCAACACCTGCG
GCTTCATCGACGAAGCCGTGCAGGAGAGCCTCGACGCGATCGGCGAGGCGCTGACCGAGAATGGCAAGGTCATCGTGACC
GGCTGTCTCGGGGCGAAGAAGGATGCGGCGGGGCACGACATCGTGTCGTCGGTGCACCCGAAAGTGCTGGCCGTGACCGG
CCCGCACGCCCTTGGCGAAGTCATGCAGGCGGTGCACACGCACCTGCCCAAGCCGCATGACCCCTTTACCGACCTGGTGC
CGGCGGCCGGCATCAAGCTGACGCCCAAGCACTACGCCTACCTGAAGATTTCCGAAGGCTGCAACCATCGCTGCTCGTTC
TGCATCATCCCGTCGATGCGCGGCGACCTGGTGTCGCGTCCCGTCGCCGAAGTCATGCTGGAAGCCGAGAACCTGTTCAA
GGCCGGCGTGAAGGAATTGCTGGTGATCTCGCAGGACACCAGCGCTTACGGCGTGGACGTGAAGTACCGCACCGGCTTCT
GGAACGGACGTCCGCTCAAGACCCGCATGACCGAACTGGTCGCGGCGCTGGGTGAACTGGCGGCCCAGTACGGTGCGTGG
GTGCGCCTGCACTACGTGTACCCGTACCCGCACGTCGACGAGATCATTCCGTTGATGAACGGCGGTCACGTGCTGCCGTA
CCTGGACGTGCCGCTGCAACACGCGCACCCCGACGTGCTCAAGCGCATGAAGCGTCCGGCCAATGCCGAGAAGACGCTGG
ACCGCATCCGCGCCTGGCGCGAGGTGTGCCCGGACCTGACGATCCGCAGCACTTTTATCGCCGGCTTCCCGGGCGAAACT
GAAGCGGAATTCGAGACCCTGCTCGATTTCATCGCCGAAGCCGAGCTGGACCGCGTGGGCTGCTTTGCGTACTCCCCGGT
GGAAGGCGCGACGGCCAACGACCTGCCGGGCGCCTTGCCCGACGAAGTCCGCGAGGAACGCCGCGCGCGCTTCATGGAGG
TGGCCGAGGAAGTCTCCGCACGCCGCCTGCAGCGCAAGGTTGGCCAGACGCTGCGCGTGCTGATCGACGAGGTGAACCAG
GACGGCGGCATCGGCCGGTCTTCGGCGGATGCGCCGGAAATCGACGGCCTGGTCTATATCGCGCCGCCGGCCAAGCCGTC
GCAGCGCTATCGCGCCGGGGACTTCGTTCAGGTGAAGATCACTGGCGCTGATGGTCACGACTTGTGGGGCGAGATTTGA

Upstream 100 bases:

>100_bases
AGCGCCGGGCGGACTTGCCCGACATCCATCGGGATTACCCGATATACTCCGCCCCGCTTGCCCCACATTCGCATTCCCCG
TGAAAAATCCTTCAGAATCA

Downstream 100 bases:

>100_bases
TGCAACGGGCTTCTGCTGCCCTTAAGTAAAAGTACGATCGTTCGATTCCGTCGCGATGCCGCTATACTGTGCACCGCAAC
ATAACCTCATGGAGAGAGAC

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MTQKDQSPRVGFVSLGCPKALVDSEQIITQLRAEGYAISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALTENGKVIVT
GCLGAKKDAAGHDIVSSVHPKVLAVTGPHALGEVMQAVHTHLPKPHDPFTDLVPAAGIKLTPKHYAYLKISEGCNHRCSF
CIIPSMRGDLVSRPVAEVMLEAENLFKAGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVAALGELAAQYGAW
VRLHYVYPYPHVDEIIPLMNGGHVLPYLDVPLQHAHPDVLKRMKRPANAEKTLDRIRAWREVCPDLTIRSTFIAGFPGET
EAEFETLLDFIAEAELDRVGCFAYSPVEGATANDLPGALPDEVREERRARFMEVAEEVSARRLQRKVGQTLRVLIDEVNQ
DGGIGRSSADAPEIDGLVYIAPPAKPSQRYRAGDFVQVKITGADGHDLWGEI

Sequences:

>Translated_452_residues
MTQKDQSPRVGFVSLGCPKALVDSEQIITQLRAEGYAISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALTENGKVIVT
GCLGAKKDAAGHDIVSSVHPKVLAVTGPHALGEVMQAVHTHLPKPHDPFTDLVPAAGIKLTPKHYAYLKISEGCNHRCSF
CIIPSMRGDLVSRPVAEVMLEAENLFKAGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVAALGELAAQYGAW
VRLHYVYPYPHVDEIIPLMNGGHVLPYLDVPLQHAHPDVLKRMKRPANAEKTLDRIRAWREVCPDLTIRSTFIAGFPGET
EAEFETLLDFIAEAELDRVGCFAYSPVEGATANDLPGALPDEVREERRARFMEVAEEVSARRLQRKVGQTLRVLIDEVNQ
DGGIGRSSADAPEIDGLVYIAPPAKPSQRYRAGDFVQVKITGADGHDLWGEI
>Mature_451_residues
TQKDQSPRVGFVSLGCPKALVDSEQIITQLRAEGYAISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALTENGKVIVTG
CLGAKKDAAGHDIVSSVHPKVLAVTGPHALGEVMQAVHTHLPKPHDPFTDLVPAAGIKLTPKHYAYLKISEGCNHRCSFC
IIPSMRGDLVSRPVAEVMLEAENLFKAGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVAALGELAAQYGAWV
RLHYVYPYPHVDEIIPLMNGGHVLPYLDVPLQHAHPDVLKRMKRPANAEKTLDRIRAWREVCPDLTIRSTFIAGFPGETE
AEFETLLDFIAEAELDRVGCFAYSPVEGATANDLPGALPDEVREERRARFMEVAEEVSARRLQRKVGQTLRVLIDEVNQD
GGIGRSSADAPEIDGLVYIAPPAKPSQRYRAGDFVQVKITGADGHDLWGEI

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=411, Percent_Identity=26.2773722627737, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI28872784, Length=479, Percent_Identity=24.8434237995825, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI28872782, Length=479, Percent_Identity=24.8434237995825, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1787057, Length=451, Percent_Identity=63.8580931263858, Blast_Score=608, Evalue=1e-175,
Organism=Escherichia coli, GI1786882, Length=455, Percent_Identity=25.9340659340659, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553146, Length=483, Percent_Identity=23.3954451345756, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71996771, Length=304, Percent_Identity=28.2894736842105, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356207, Length=486, Percent_Identity=26.3374485596708, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI19922432, Length=466, Percent_Identity=26.1802575107296, Blast_Score=116, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49397; Mature: 49266

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKDQSPRVGFVSLGCPKALVDSEQIITQLRAEGYAISGTYDGADLVVVNTCGFIDEAV
CCCCCCCCCEEEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCCEEEEECCCHHHHHH
QESLDAIGEALTENGKVIVTGCLGAKKDAAGHDIVSSVHPKVLAVTGPHALGEVMQAVHT
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCHHHHHHHHHHHH
HLPKPHDPFTDLVPAAGIKLTPKHYAYLKISEGCNHRCSFCIIPSMRGDLVSRPVAEVML
HCCCCCCCHHHHHCCCCCEECCCCEEEEEECCCCCCCCCEEEECCCCCHHHHCCHHHHHH
EAENLFKAGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVAALGELAAQYGAW
HHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCE
VRLHYVYPYPHVDEIIPLMNGGHVLPYLDVPLQHAHPDVLKRMKRPANAEKTLDRIRAWR
EEEEEECCCCCHHHHHHHCCCCEECEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
EVCPDLTIRSTFIAGFPGETEAEFETLLDFIAEAELDRVGCFAYSPVEGATANDLPGALP
HHCCCCEEEHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCC
DEVREERRARFMEVAEEVSARRLQRKVGQTLRVLIDEVNQDGGIGRSSADAPEIDGLVYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
APPAKPSQRYRAGDFVQVKITGADGHDLWGEI
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TQKDQSPRVGFVSLGCPKALVDSEQIITQLRAEGYAISGTYDGADLVVVNTCGFIDEAV
CCCCCCCCEEEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCCEEEEECCCHHHHHH
QESLDAIGEALTENGKVIVTGCLGAKKDAAGHDIVSSVHPKVLAVTGPHALGEVMQAVHT
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCHHHHHHHHHHHH
HLPKPHDPFTDLVPAAGIKLTPKHYAYLKISEGCNHRCSFCIIPSMRGDLVSRPVAEVML
HCCCCCCCHHHHHCCCCCEECCCCEEEEEECCCCCCCCCEEEECCCCCHHHHCCHHHHHH
EAENLFKAGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVAALGELAAQYGAW
HHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCE
VRLHYVYPYPHVDEIIPLMNGGHVLPYLDVPLQHAHPDVLKRMKRPANAEKTLDRIRAWR
EEEEEECCCCCHHHHHHHCCCCEECEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
EVCPDLTIRSTFIAGFPGETEAEFETLLDFIAEAELDRVGCFAYSPVEGATANDLPGALP
HHCCCCEEEHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCC
DEVREERRARFMEVAEEVSARRLQRKVGQTLRVLIDEVNQDGGIGRSSADAPEIDGLVYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
APPAKPSQRYRAGDFVQVKITGADGHDLWGEI
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA