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The map label for this gene is dusC [H]

Identifier: 73541045

GI number: 73541045

Start: 1452475

End: 1453449

Strand: Direct

Name: dusC [H]

Synonym: Reut_A1351

Alternate gene names: 73541045

Gene position: 1452475-1453449 (Clockwise)

Preceding gene: 73541044

Following gene: 73541046

Centisome position: 38.16

GC content: 66.87

Gene sequence:

>975_bases
ATGAGCCGGCTATTCCTCGCCCCGATGGAGGGGCTCGCTGACTATGTGCTGCGCGACGTGCTGACAGGCCTCGGCGGCTA
CGACGGCTGCGTGTCCGAGTTCGTGCGGGTGACGGGCTCGCTGCTTCCTCCGCGCGTCTACGAGCGCGATACTCCCGAGA
TCCTGCAGGGCGGCCATACCCCGAGCGGCACCCCGATGGTGATCCAGCTGCTCGGCAGCGATCCCGAGTGGCTGGCACGC
AATGCGACTTATGCCGCGACCCTGTCGCCGCACGGTATCGACCTGAACTTCGGTTGCCCGGCCAAGGTCGTCAACCGGCA
CGGGGGCGGCGCGATGCTGCTGGCGCATCCCGAGCAACTGCATAGCATCGTTTCCGCGGTACGCGCAGCGGTCCCGCCGC
ATATCGCGGTCACGGCCAAAATGCGGCTCGGCGTTTCCGATACCGCGCTGGCGCTCGATTGCGCCACGGCGCTGGCGGAG
GGTGGGGCGGCGTCGCTGGTGGTGCACGCCCGCACGCGTGACCACGGCTACCGGCCACCGGCGCACTGGGAATGGATCGC
CCGCATCGCCGACACCGTCGATATCCCCGTCATTGCCAACGGTGACGTCTGGTCGGTTGCCGACTGGGAGCGTTGCCGCG
CGGTCAGTGGCTGCGCCGATGTGATGATCGGCCGCGGCGCGGTATCGGATCCGTTCCTGGCCCTGCGTATCCGAGGCCTC
ATGGACAGCACGCCATCCGATGCTGATTGGCCGCTCGTGCTGCAGCAGATTGCGGCTTACCTGAGGAAGCTGCATGACCG
GATCTCATCCTGCCATGAGCACGGACGGGTGAAGTTGTGGCTCAGCTATCTCAAGCGGACCTGGCCGCAAGCCACGGCGC
TGCACGATGCGATCCGCAGGATCCACGATTCGGCCCAAATCGAGCAGGTACTGCAGAGTGCCTCTGATGCCACGCAGGGG
ATCGCCCCGGCATAG

Upstream 100 bases:

>100_bases
GGACTCGCCTGCAGCGCGATATCCCGATGATTCCAAGCCTTCCCCGTTCCACTTTGTCTGCCCCGGGTTCCGGCGTGACC
GCGCTGGCTCGAGCCTGACC

Downstream 100 bases:

>100_bases
GGCCAGAGCCGCCTGTCTTGCCGTGTGCCGCGTCAGGCGCCGTTTGACTGCGGCAAACGGTTAAAATCCGGGTTGCTTGC
AGCGCCGGGCGGACTTGCCC

Product: dihydrouridine synthase, DuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSRLFLAPMEGLADYVLRDVLTGLGGYDGCVSEFVRVTGSLLPPRVYERDTPEILQGGHTPSGTPMVIQLLGSDPEWLAR
NATYAATLSPHGIDLNFGCPAKVVNRHGGGAMLLAHPEQLHSIVSAVRAAVPPHIAVTAKMRLGVSDTALALDCATALAE
GGAASLVVHARTRDHGYRPPAHWEWIARIADTVDIPVIANGDVWSVADWERCRAVSGCADVMIGRGAVSDPFLALRIRGL
MDSTPSDADWPLVLQQIAAYLRKLHDRISSCHEHGRVKLWLSYLKRTWPQATALHDAIRRIHDSAQIEQVLQSASDATQG
IAPA

Sequences:

>Translated_324_residues
MSRLFLAPMEGLADYVLRDVLTGLGGYDGCVSEFVRVTGSLLPPRVYERDTPEILQGGHTPSGTPMVIQLLGSDPEWLAR
NATYAATLSPHGIDLNFGCPAKVVNRHGGGAMLLAHPEQLHSIVSAVRAAVPPHIAVTAKMRLGVSDTALALDCATALAE
GGAASLVVHARTRDHGYRPPAHWEWIARIADTVDIPVIANGDVWSVADWERCRAVSGCADVMIGRGAVSDPFLALRIRGL
MDSTPSDADWPLVLQQIAAYLRKLHDRISSCHEHGRVKLWLSYLKRTWPQATALHDAIRRIHDSAQIEQVLQSASDATQG
IAPA
>Mature_323_residues
SRLFLAPMEGLADYVLRDVLTGLGGYDGCVSEFVRVTGSLLPPRVYERDTPEILQGGHTPSGTPMVIQLLGSDPEWLARN
ATYAATLSPHGIDLNFGCPAKVVNRHGGGAMLLAHPEQLHSIVSAVRAAVPPHIAVTAKMRLGVSDTALALDCATALAEG
GAASLVVHARTRDHGYRPPAHWEWIARIADTVDIPVIANGDVWSVADWERCRAVSGCADVMIGRGAVSDPFLALRIRGLM
DSTPSDADWPLVLQQIAAYLRKLHDRISSCHEHGRVKLWLSYLKRTWPQATALHDAIRRIHDSAQIEQVLQSASDATQGI
APA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=168, Percent_Identity=31.547619047619, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI239788483, Length=227, Percent_Identity=27.7533039647577, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI239788462, Length=227, Percent_Identity=27.7533039647577, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI8923374, Length=152, Percent_Identity=34.8684210526316, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI40807366, Length=198, Percent_Identity=29.7979797979798, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1788462, Length=312, Percent_Identity=41.6666666666667, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI1789660, Length=311, Percent_Identity=31.1897106109325, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI145693211, Length=214, Percent_Identity=28.9719626168224, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=213, Percent_Identity=28.6384976525822, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17507177, Length=147, Percent_Identity=32.6530612244898, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17543114, Length=153, Percent_Identity=27.4509803921569, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323437, Length=176, Percent_Identity=30.1136363636364, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=171, Percent_Identity=28.0701754385965, Blast_Score=76, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323433, Length=182, Percent_Identity=26.9230769230769, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585320, Length=230, Percent_Identity=31.7391304347826, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24580595, Length=233, Percent_Identity=27.8969957081545, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920448, Length=233, Percent_Identity=27.8969957081545, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921524, Length=174, Percent_Identity=31.6091954022989, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI45549423, Length=139, Percent_Identity=35.9712230215827, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34981; Mature: 34850

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLFLAPMEGLADYVLRDVLTGLGGYDGCVSEFVRVTGSLLPPRVYERDTPEILQGGHT
CCCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCCCHHHHCCCCC
PSGTPMVIQLLGSDPEWLARNATYAATLSPHGIDLNFGCPAKVVNRHGGGAMLLAHPEQL
CCCCCEEEEEECCCHHHHHCCCEEEEEECCCCEEECCCCCHHHHHCCCCCEEEEECHHHH
HSIVSAVRAAVPPHIAVTAKMRLGVSDTALALDCATALAEGGAASLVVHARTRDHGYRPP
HHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
AHWEWIARIADTVDIPVIANGDVWSVADWERCRAVSGCADVMIGRGAVSDPFLALRIRGL
HHHHHHHHHHHHCCCCEEECCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
MDSTPSDADWPLVLQQIAAYLRKLHDRISSCHEHGRVKLWLSYLKRTWPQATALHDAIRR
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHH
IHDSAQIEQVLQSASDATQGIAPA
HHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SRLFLAPMEGLADYVLRDVLTGLGGYDGCVSEFVRVTGSLLPPRVYERDTPEILQGGHT
CCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCCCHHHHCCCCC
PSGTPMVIQLLGSDPEWLARNATYAATLSPHGIDLNFGCPAKVVNRHGGGAMLLAHPEQL
CCCCCEEEEEECCCHHHHHCCCEEEEEECCCCEEECCCCCHHHHHCCCCCEEEEECHHHH
HSIVSAVRAAVPPHIAVTAKMRLGVSDTALALDCATALAEGGAASLVVHARTRDHGYRPP
HHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
AHWEWIARIADTVDIPVIANGDVWSVADWERCRAVSGCADVMIGRGAVSDPFLALRIRGL
HHHHHHHHHHHHCCCCEEECCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
MDSTPSDADWPLVLQQIAAYLRKLHDRISSCHEHGRVKLWLSYLKRTWPQATALHDAIRR
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHH
IHDSAQIEQVLQSASDATQGIAPA
HHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9555876 [H]