Click here to switch to the map view.

The map label for this gene is phbC [H]

Identifier: 73541041

GI number: 73541041

Start: 1447474

End: 1449249

Strand: Direct

Name: phbC [H]

Synonym: Reut_A1347

Alternate gene names: 73541041

Gene position: 1447474-1449249 (Clockwise)

Preceding gene: 73541039

Following gene: 73541042

Centisome position: 38.03

GC content: 64.7

Gene sequence:

>1776_bases
ATGGCGACCGGCAAAGGTGCGGCAGCTTCCTCAACGGAAGGCAAGTCCCAACCGTTCAAGTTCCCGCCGGGGCCATTGGA
TCCAGCAACCTGGCTGGAGTGGTCCCGTCAGTGGCAAGGCCCTGAGGGCAACGGCGGCACGGTTCCCGGTGGTTTTCCGG
GTTTCGAGGCCTTCGCGGCGAGCCCGCTTGCGGGTGTCAAGATCGACCCGGCCCAGCTCGCGGAAATCCAGCAGCGCTAC
ATGCGCGATTTCACCGAACTGTGGCGCGGCCTGGCGGGAGGCGATACCGAATCCGCGGGCAAGCTCCACGATCGTCGCTT
TGCCAGCGAGGCCTGGCACAAGAACGCTCCGTACCGCTACACCGCGGCGTTCTACCTGCTCAATGCGCGCGCCCTGACGG
AACTGGCCGATGCAGTCGAGGCGGATCCCAAGACACGCCAGCGTATCCGCTTCGCCGTGTCGCAATGGGTCGATGCGATG
TCGCCGGCCAATTTCCTGGCGACGAATCCCGATGCGCAGAACCGGCTGATCGAGTCGCGCGGTGAATCGCTGCGCGCCGG
CATGCGGAACATGCTCGAGGACCTGACGCGCGGCAAGATTTCGCAGACCGACGAGACCGCGTTCGAAGTCGGCCGCAACA
TGGCCGTCACCGAAGGCGCGGTGGTCTTCGAAAACGAATTCTTCCAGCTGCTGCAATACAAGCCGCTGACGGACAAGGTC
TATACGCGTCCGCTGCTGCTGGTGCCGCCTTGCATCAACAAGTTCTACATTCTCGACCTGCAGCCGGAAGGCTCGCTGGT
GCGGTACGCTGTCGAGCAGGGCCATACCGTGTTCCTGGTGTCGTGGCGCAACCCGGATGCCAGCATGGCGGGATGCACGT
GGGACGACTACATCGAGAACGCGGCCATCCGCGCCATCGAAGTGGTGCGCGATATCAGCGGCCAGGACAAGATCAACACG
CTTGGCTTCTGCGTGGGCGGGACCATCATTTCGACCGCGCTGGCCGTGCTGGCCGCGCGTGGCGAACACCCGGTGGCCAG
CCTGACGCTGCTGACCACGCTGCTGGACTTCACCGATACCGGCATCCTTGACGTATTCGTCGACGAACCTCACGTGCAGT
TGCGCGAAGCCACGCTCGGCGGCGCGTCCGGTGGCCTGCTGCGCGGCGTGGAACTGGCCAATACGTTCTCGTTCCTGCGC
CCGAACGACCTGGTCTGGAACTACGTGGTCGACAACTACCTGAAGGGCAACACGCCGGTGCCGTTCGACCTGCTGTTCTG
GAACGGCGACGCCACCAACCTGCCGGGGCCGTGGTATTGCTGGTACCTGCGCCACACCTATCTGCAGAACGAACTGAAGG
AGCCGGGCAAGCTGACCGTGTGCAACGAGCCGGTGGACCTCGGCGCCATCAACGTGCCGACCTATATCTACGGCTCGCGC
GAGGACCACATCGTGCCGTGGACGGCGGCGTATGCGTCGACCGCATTGCTCAAGAATGACCTGCGCTTCGTGCTCGGGGC
GTCGGGGCACATTGCCGGCGTCATCAACCCGCCCGCCAAGAAAAAGCGCAGCCACTGGACCAACGATGCGTTGCCGGAAT
CGGCGCAAGACTGGCTGGCCGGTGCCGAGGAGCACCATGGCAGCTGGTGGCCGGACTGGATGACGTGGCTGGGCAAGCAG
GCCGGCGCCAAGCGCGCCGCACCGACCGAATACGGCAGCAAGCGTTACGCTGCCATCGAACCCGCGCCGGGCCGTTACGT
CAAGGCCAAGGCATGA

Upstream 100 bases:

>100_bases
GTCAGGTCCATCCGGATTGCGTCTCCAGATGCATAAGTGCCGGCCAGGGCAATGTCCCGGAGCCGGTCTGACATAGTGAC
GGCAGAGAGACAATTACATC

Downstream 100 bases:

>100_bases
CTCTTGCGAGCGGTCCCGCTATTCCGGGACCTGCCCGCTGACCCCTAAGCTATTCATTGAGAGGACTCCAAATGACCGAT
ATCGTCATCGTTTCCGCCGC

Product: Poly(R)-hydroxyalkanoic acid synthase, class I

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MATGKGAAASSTEGKSQPFKFPPGPLDPATWLEWSRQWQGPEGNGGTVPGGFPGFEAFAASPLAGVKIDPAQLAEIQQRY
MRDFTELWRGLAGGDTESAGKLHDRRFASEAWHKNAPYRYTAAFYLLNARALTELADAVEADPKTRQRIRFAVSQWVDAM
SPANFLATNPDAQNRLIESRGESLRAGMRNMLEDLTRGKISQTDETAFEVGRNMAVTEGAVVFENEFFQLLQYKPLTDKV
YTRPLLLVPPCINKFYILDLQPEGSLVRYAVEQGHTVFLVSWRNPDASMAGCTWDDYIENAAIRAIEVVRDISGQDKINT
LGFCVGGTIISTALAVLAARGEHPVASLTLLTTLLDFTDTGILDVFVDEPHVQLREATLGGASGGLLRGVELANTFSFLR
PNDLVWNYVVDNYLKGNTPVPFDLLFWNGDATNLPGPWYCWYLRHTYLQNELKEPGKLTVCNEPVDLGAINVPTYIYGSR
EDHIVPWTAAYASTALLKNDLRFVLGASGHIAGVINPPAKKKRSHWTNDALPESAQDWLAGAEEHHGSWWPDWMTWLGKQ
AGAKRAAPTEYGSKRYAAIEPAPGRYVKAKA

Sequences:

>Translated_591_residues
MATGKGAAASSTEGKSQPFKFPPGPLDPATWLEWSRQWQGPEGNGGTVPGGFPGFEAFAASPLAGVKIDPAQLAEIQQRY
MRDFTELWRGLAGGDTESAGKLHDRRFASEAWHKNAPYRYTAAFYLLNARALTELADAVEADPKTRQRIRFAVSQWVDAM
SPANFLATNPDAQNRLIESRGESLRAGMRNMLEDLTRGKISQTDETAFEVGRNMAVTEGAVVFENEFFQLLQYKPLTDKV
YTRPLLLVPPCINKFYILDLQPEGSLVRYAVEQGHTVFLVSWRNPDASMAGCTWDDYIENAAIRAIEVVRDISGQDKINT
LGFCVGGTIISTALAVLAARGEHPVASLTLLTTLLDFTDTGILDVFVDEPHVQLREATLGGASGGLLRGVELANTFSFLR
PNDLVWNYVVDNYLKGNTPVPFDLLFWNGDATNLPGPWYCWYLRHTYLQNELKEPGKLTVCNEPVDLGAINVPTYIYGSR
EDHIVPWTAAYASTALLKNDLRFVLGASGHIAGVINPPAKKKRSHWTNDALPESAQDWLAGAEEHHGSWWPDWMTWLGKQ
AGAKRAAPTEYGSKRYAAIEPAPGRYVKAKA
>Mature_590_residues
ATGKGAAASSTEGKSQPFKFPPGPLDPATWLEWSRQWQGPEGNGGTVPGGFPGFEAFAASPLAGVKIDPAQLAEIQQRYM
RDFTELWRGLAGGDTESAGKLHDRRFASEAWHKNAPYRYTAAFYLLNARALTELADAVEADPKTRQRIRFAVSQWVDAMS
PANFLATNPDAQNRLIESRGESLRAGMRNMLEDLTRGKISQTDETAFEVGRNMAVTEGAVVFENEFFQLLQYKPLTDKVY
TRPLLLVPPCINKFYILDLQPEGSLVRYAVEQGHTVFLVSWRNPDASMAGCTWDDYIENAAIRAIEVVRDISGQDKINTL
GFCVGGTIISTALAVLAARGEHPVASLTLLTTLLDFTDTGILDVFVDEPHVQLREATLGGASGGLLRGVELANTFSFLRP
NDLVWNYVVDNYLKGNTPVPFDLLFWNGDATNLPGPWYCWYLRHTYLQNELKEPGKLTVCNEPVDLGAINVPTYIYGSRE
DHIVPWTAAYASTALLKNDLRFVLGASGHIAGVINPPAKKKRSHWTNDALPESAQDWLAGAEEHHGSWWPDWMTWLGKQA
GAKRAAPTEYGSKRYAAIEPAPGRYVKAKA

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 65238; Mature: 65107

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGKGAAASSTEGKSQPFKFPPGPLDPATWLEWSRQWQGPEGNGGTVPGGFPGFEAFAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHC
SPLAGVKIDPAQLAEIQQRYMRDFTELWRGLAGGDTESAGKLHDRRFASEAWHKNAPYRY
CCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEE
TAAFYLLNARALTELADAVEADPKTRQRIRFAVSQWVDAMSPANFLATNPDAQNRLIESR
EHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHC
GESLRAGMRNMLEDLTRGKISQTDETAFEVGRNMAVTEGAVVFENEFFQLLQYKPLTDKV
CHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEECCEEEEHHHHHHHHHCCCCCHHH
YTRPLLLVPPCINKFYILDLQPEGSLVRYAVEQGHTVFLVSWRNPDASMAGCTWDDYIEN
HCCCEEEECCCCCCEEEEEECCCCCEEHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHH
AAIRAIEVVRDISGQDKINTLGFCVGGTIISTALAVLAARGEHPVASLTLLTTLLDFTDT
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
GILDVFVDEPHVQLREATLGGASGGLLRGVELANTFSFLRPNDLVWNYVVDNYLKGNTPV
CEEEEEECCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
PFDLLFWNGDATNLPGPWYCWYLRHTYLQNELKEPGKLTVCNEPVDLGAINVPTYIYGSR
CEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHCCCCCEEEECCCCCCCEECCCEEEECCC
EDHIVPWTAAYASTALLKNDLRFVLGASGHIAGVINPPAKKKRSHWTNDALPESAQDWLA
CCCCCEEHHHHHHHHHHHHCCCEEECCCCCEEEEECCCHHHHHCCCCCCCCCCHHHHHHC
GAEEHHGSWWPDWMTWLGKQAGAKRAAPTEYGSKRYAAIEPAPGRYVKAKA
CCHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCEEECCC
>Mature Secondary Structure 
ATGKGAAASSTEGKSQPFKFPPGPLDPATWLEWSRQWQGPEGNGGTVPGGFPGFEAFAA
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHC
SPLAGVKIDPAQLAEIQQRYMRDFTELWRGLAGGDTESAGKLHDRRFASEAWHKNAPYRY
CCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEE
TAAFYLLNARALTELADAVEADPKTRQRIRFAVSQWVDAMSPANFLATNPDAQNRLIESR
EHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHC
GESLRAGMRNMLEDLTRGKISQTDETAFEVGRNMAVTEGAVVFENEFFQLLQYKPLTDKV
CHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEECCEEEEHHHHHHHHHCCCCCHHH
YTRPLLLVPPCINKFYILDLQPEGSLVRYAVEQGHTVFLVSWRNPDASMAGCTWDDYIEN
HCCCEEEECCCCCCEEEEEECCCCCEEHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHH
AAIRAIEVVRDISGQDKINTLGFCVGGTIISTALAVLAARGEHPVASLTLLTTLLDFTDT
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
GILDVFVDEPHVQLREATLGGASGGLLRGVELANTFSFLRPNDLVWNYVVDNYLKGNTPV
CEEEEEECCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
PFDLLFWNGDATNLPGPWYCWYLRHTYLQNELKEPGKLTVCNEPVDLGAINVPTYIYGSR
CEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHCCCCCEEEECCCCCCCEECCCEEEECCC
EDHIVPWTAAYASTALLKNDLRFVLGASGHIAGVINPPAKKKRSHWTNDALPESAQDWLA
CCCCCEEHHHHHHHHHHHHCCCEEECCCCCEEEEECCCHHHHHCCCCCCCCCCHHHHHHC
GAEEHHGSWWPDWMTWLGKQAGAKRAAPTEYGSKRYAAIEPAPGRYVKAKA
CCHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2670936; 1987116 [H]