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The map label for this gene is pdhB [H]

Identifier: 73540998

GI number: 73540998

Start: 1397947

End: 1399611

Strand: Direct

Name: pdhB [H]

Synonym: Reut_A1304

Alternate gene names: 73540998

Gene position: 1397947-1399611 (Clockwise)

Preceding gene: 73540997

Following gene: 73540999

Centisome position: 36.72

GC content: 66.19

Gene sequence:

>1665_bases
ATGAGTCAAGCGATTGAAATCAAGGTGCCGGACATCGGCGACTATGACGCCGTTCCCGTCATTGAAGTGCATGTGAAACC
CGGCGACAGCATCAATGCGGAAGACGCGCTGGTCACGCTGGAATCGGACAAGGCCACCATGGACGTGCCCTCGCCGCAGG
CCGGCGTGGTCAAGGAAGTCCGCATCAAGGTGGGCGACAACGTATCCGAAGGCTCCGTGCTGGTGATGCTGGAAGCCGCT
GGCGCTGCCGCGCCTGCGCCGGCTCCCGCAGTTGCCGCACCGGCGCCTGCCCCCGCCCAGGCTGCCGCGCCCGCACCGGC
TCCGGCCGCTGCCCCGGCGCCCGCCGCTGCTCCGGCAGCCGGCGGCACCATTGAAGTCAAGGTGCCGGATATCGGTGATT
ACGACGCTGTTCCCGTGATCGAAGTCCACGTGAAGCCGGGCGACACGATCAACCCCGAAGATGCCGTCGTCACGCTGGAA
TCGGACAAGGCCACGATGGACGTGCCGTCCCCGCAAGGCGGCGTGGTCAAGGAAGTCAAGGTCAAGGTCGGCGACAACGT
GTCCGAGGGCACGCTGCTGATCCTGCTGGAAGGCGCCGCTGGCGCCGCCGCACCGGCTCCGGCCGCTGCAGCACCCGCTC
CGGCTCCCGCGGCAGCACCTGCCCAGGCAGCGGCCCCGGCGCCCGTTGCCGCTGCGCCGGCTCCGGCAGCCGCCGCCTCA
GCGCCTGTCGGCGTGACCGGCAAGGCCGCGCACGCATCGCCGTCGGTGCGCAAGTTCGCGCGCGAACTCGGTGTGGATGT
TTCCCGCGTGCCGGGCACGGGACCGAAGAACCGCATCACCCAGGAAGACGTACAGCGCTACGTCAAGGGCGTGATGACCG
GCCAGGCTGCCGCGCCGGCACAGGCTGCCGCGGCTGGTGGCGGTGGTGGCGAGCTTGGCCTGCTGCCGTGGCCGAAGGTC
GATTTCACGCGCTTCGGCGAGGTGGAAAGCAAGGCCCTGTCGCGCATCAAGAAGATCTCGGGTGCAAACCTGCACCGCAA
CTGGGTCATGATTCCACACGTCACCAACCATGACGAAGCGGACATCACCGACCTGGAAGCCTTCCGCGTCCAACTCAACA
AGGAAAACGAGAAGTCCGGCGTCAAGGTCACGATGCTGGCGTTCATGATCAAGGCCACTGTGGCGGCGCTGAAGAAGTTC
CCGAACTTCAATGCCTCGCTCGACGGTGACAACCTGGTGCTGAAGAAATACTTCAACATCGGTTTCGCGGCCGATACGCC
GAACGGCCTCGTCGTGCCGGTCATCAAGGACGCCGACAAGAAGGGCGTGCTCGAGATCAGCCAGGAAATGAGCGAGCTGG
CCAAGCTCGCGCGCGACGGCAAGCTCAAGCCCGACCAGATGCAGGGCGGCTGCTTCTCGATCTCGTCGCTGGGCGGCATC
GGCGGCACGTACTTCACGCCGATCATCAACGCGCCGGAAGTGGCCATCATGGGCGTGTGCAAGTCGTACATGAAGCCGGT
CTGGGACGGCAAGCAGTTCGCGCCGCGCCTGACGCTGCCGCTGTCGCTGTCGTGGGACCACCGCGTCATCGACGGCGCCG
AGGCGGCCCGCTTCAATACGTACTTCGGGCAACTGCTGGCGGACTTCCGTCGCATCCTTCTCTAA

Upstream 100 bases:

>100_bases
GGTGGCGCCTTGCGGCACAGCGGCGTGACAGAAGCGGGCGGCGAGGGTAATCTCGCCGCTTGCGGATGTCGTGCGCGCCG
GCCTGCCCAGGAGACACTGA

Downstream 100 bases:

>100_bases
GGGGCCGCGGCCATGTCCGGAACGGGCATGGCCATGGTGCTTGCAGTCGAGCGAGGCAAGCCATGACTACCTGTGTCGTC
GTCAGAAAGGGTGCAGAGGT

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSQAIEIKVPDIGDYDAVPVIEVHVKPGDSINAEDALVTLESDKATMDVPSPQAGVVKEVRIKVGDNVSEGSVLVMLEAA
GAAAPAPAPAVAAPAPAPAQAAAPAPAPAAAPAPAAAPAAGGTIEVKVPDIGDYDAVPVIEVHVKPGDTINPEDAVVTLE
SDKATMDVPSPQGGVVKEVKVKVGDNVSEGTLLILLEGAAGAAAPAPAAAAPAPAPAAAPAQAAAPAPVAAAPAPAAAAS
APVGVTGKAAHASPSVRKFARELGVDVSRVPGTGPKNRITQEDVQRYVKGVMTGQAAAPAQAAAAGGGGGELGLLPWPKV
DFTRFGEVESKALSRIKKISGANLHRNWVMIPHVTNHDEADITDLEAFRVQLNKENEKSGVKVTMLAFMIKATVAALKKF
PNFNASLDGDNLVLKKYFNIGFAADTPNGLVVPVIKDADKKGVLEISQEMSELAKLARDGKLKPDQMQGGCFSISSLGGI
GGTYFTPIINAPEVAIMGVCKSYMKPVWDGKQFAPRLTLPLSLSWDHRVIDGAEAARFNTYFGQLLADFRRILL

Sequences:

>Translated_554_residues
MSQAIEIKVPDIGDYDAVPVIEVHVKPGDSINAEDALVTLESDKATMDVPSPQAGVVKEVRIKVGDNVSEGSVLVMLEAA
GAAAPAPAPAVAAPAPAPAQAAAPAPAPAAAPAPAAAPAAGGTIEVKVPDIGDYDAVPVIEVHVKPGDTINPEDAVVTLE
SDKATMDVPSPQGGVVKEVKVKVGDNVSEGTLLILLEGAAGAAAPAPAAAAPAPAPAAAPAQAAAPAPVAAAPAPAAAAS
APVGVTGKAAHASPSVRKFARELGVDVSRVPGTGPKNRITQEDVQRYVKGVMTGQAAAPAQAAAAGGGGGELGLLPWPKV
DFTRFGEVESKALSRIKKISGANLHRNWVMIPHVTNHDEADITDLEAFRVQLNKENEKSGVKVTMLAFMIKATVAALKKF
PNFNASLDGDNLVLKKYFNIGFAADTPNGLVVPVIKDADKKGVLEISQEMSELAKLARDGKLKPDQMQGGCFSISSLGGI
GGTYFTPIINAPEVAIMGVCKSYMKPVWDGKQFAPRLTLPLSLSWDHRVIDGAEAARFNTYFGQLLADFRRILL
>Mature_553_residues
SQAIEIKVPDIGDYDAVPVIEVHVKPGDSINAEDALVTLESDKATMDVPSPQAGVVKEVRIKVGDNVSEGSVLVMLEAAG
AAAPAPAPAVAAPAPAPAQAAAPAPAPAAAPAPAAAPAAGGTIEVKVPDIGDYDAVPVIEVHVKPGDTINPEDAVVTLES
DKATMDVPSPQGGVVKEVKVKVGDNVSEGTLLILLEGAAGAAAPAPAAAAPAPAPAAAPAQAAAPAPVAAAPAPAAAASA
PVGVTGKAAHASPSVRKFARELGVDVSRVPGTGPKNRITQEDVQRYVKGVMTGQAAAPAQAAAAGGGGGELGLLPWPKVD
FTRFGEVESKALSRIKKISGANLHRNWVMIPHVTNHDEADITDLEAFRVQLNKENEKSGVKVTMLAFMIKATVAALKKFP
NFNASLDGDNLVLKKYFNIGFAADTPNGLVVPVIKDADKKGVLEISQEMSELAKLARDGKLKPDQMQGGCFSISSLGGIG
GTYFTPIINAPEVAIMGVCKSYMKPVWDGKQFAPRLTLPLSLSWDHRVIDGAEAARFNTYFGQLLADFRRILL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=452, Percent_Identity=29.646017699115, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI31711992, Length=425, Percent_Identity=31.7647058823529, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI203098816, Length=441, Percent_Identity=27.891156462585, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI203098753, Length=447, Percent_Identity=27.5167785234899, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=31.858407079646, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI260898739, Length=156, Percent_Identity=37.1794871794872, Blast_Score=97, Evalue=6e-20,
Organism=Escherichia coli, GI1786305, Length=555, Percent_Identity=52.972972972973, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI1786946, Length=435, Percent_Identity=30.1149425287356, Blast_Score=180, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=434, Percent_Identity=30.8755760368664, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17560088, Length=439, Percent_Identity=30.9794988610478, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI25146366, Length=413, Percent_Identity=30.0242130750605, Blast_Score=153, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17538894, Length=314, Percent_Identity=31.2101910828025, Blast_Score=126, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=410, Percent_Identity=29.2682926829268, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324258, Length=432, Percent_Identity=27.7777777777778, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI18859875, Length=444, Percent_Identity=31.0810810810811, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24582497, Length=221, Percent_Identity=34.841628959276, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI20129315, Length=203, Percent_Identity=37.4384236453202, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24645909, Length=207, Percent_Identity=33.3333333333333, Blast_Score=115, Evalue=7e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56912; Mature: 56780

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAIEIKVPDIGDYDAVPVIEVHVKPGDSINAEDALVTLESDKATMDVPSPQAGVVKEV
CCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEECCCCCCCCCEEEE
RIKVGDNVSEGSVLVMLEAAGAAAPAPAPAVAAPAPAPAQAAAPAPAPAAAPAPAAAPAA
EEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGTIEVKVPDIGDYDAVPVIEVHVKPGDTINPEDAVVTLESDKATMDVPSPQGGVVKEVK
CCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCEEEEE
VKVGDNVSEGTLLILLEGAAGAAAPAPAAAAPAPAPAAAPAQAAAPAPVAAAPAPAAAAS
EEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCC
APVGVTGKAAHASPSVRKFARELGVDVSRVPGTGPKNRITQEDVQRYVKGVMTGQAAAPA
CCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCH
QAAAAGGGGGELGLLPWPKVDFTRFGEVESKALSRIKKISGANLHRNWVMIPHVTNHDEA
HHHHCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCEECCEEEECCCCCCCCC
DITDLEAFRVQLNKENEKSGVKVTMLAFMIKATVAALKKFPNFNASLDGDNLVLKKYFNI
CCHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
GFAADTPNGLVVPVIKDADKKGVLEISQEMSELAKLARDGKLKPDQMQGGCFSISSLGGI
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEECCCCCC
GGTYFTPIINAPEVAIMGVCKSYMKPVWDGKQFAPRLTLPLSLSWDHRVIDGAEAARFNT
CCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCEECCCHHHHHHH
YFGQLLADFRRILL
HHHHHHHHHHHHHC
>Mature Secondary Structure 
SQAIEIKVPDIGDYDAVPVIEVHVKPGDSINAEDALVTLESDKATMDVPSPQAGVVKEV
CCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEECCCCCCCCCEEEE
RIKVGDNVSEGSVLVMLEAAGAAAPAPAPAVAAPAPAPAQAAAPAPAPAAAPAPAAAPAA
EEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGTIEVKVPDIGDYDAVPVIEVHVKPGDTINPEDAVVTLESDKATMDVPSPQGGVVKEVK
CCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCEEEEE
VKVGDNVSEGTLLILLEGAAGAAAPAPAAAAPAPAPAAAPAQAAAPAPVAAAPAPAAAAS
EEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCC
APVGVTGKAAHASPSVRKFARELGVDVSRVPGTGPKNRITQEDVQRYVKGVMTGQAAAPA
CCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCH
QAAAAGGGGGELGLLPWPKVDFTRFGEVESKALSRIKKISGANLHRNWVMIPHVTNHDEA
HHHHCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCEECCEEEECCCCCCCCC
DITDLEAFRVQLNKENEKSGVKVTMLAFMIKATVAALKKFPNFNASLDGDNLVLKKYFNI
CCHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
GFAADTPNGLVVPVIKDADKKGVLEISQEMSELAKLARDGKLKPDQMQGGCFSISSLGGI
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEECCCCCC
GGTYFTPIINAPEVAIMGVCKSYMKPVWDGKQFAPRLTLPLSLSWDHRVIDGAEAARFNT
CCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCEECCCHHHHHHH
YFGQLLADFRRILL
HHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]