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The map label for this gene is ribH [H]

Identifier: 73540473

GI number: 73540473

Start: 834397

End: 834900

Strand: Reverse

Name: ribH [H]

Synonym: Reut_A0769

Alternate gene names: 73540473

Gene position: 834900-834397 (Counterclockwise)

Preceding gene: 229220876

Following gene: 73540472

Centisome position: 21.93

GC content: 65.48

Gene sequence:

>504_bases
ATGGATCACGGCTTCTACCCCAGCAACCTCGACGGTGAAGGCCTGCGTATCGGCATCGTGCAGGCCCGCTTCAACGAGCC
GGTCTGCGCCGAACTGCTCGAAGCCTGCGTGGCCGAGCTCGAAAAGCTCGGCGTCGAAGGCGAAGACACCCTGGTGGTGA
CCGTGCCTGGCGCGCTGGAAGTGCCGCTGGCACTGCAGAAGATGGCTGAAAGCGGCCAGTTCGACGCACTGGTGGCGCTG
GGCGCCGTGGTGCGCGGCGAGACCTACCATTTCGAGCTGGTCTCGAACGAGTCGGGTGCCGGCATCACGCGCGTCGGCCT
GGATTTCAACATGCCGGTCGCCAACGGCATCCTGACCGTCGATACCGACGAGCAGGCCCACGCCCGCACGCGCGAAAAGG
GCCGCGACTGCGCCCGCGCCGCCGTGGAAATGGCCAACCTGGTTGTCGCGCTCGATTCGCTGCGCGATCAGGAAGATGAA
GAAGACGAGGACGACGATGAGTGA

Upstream 100 bases:

>100_bases
ACCGGCTACCAGCCCATGAACGGGCAGGCCAAGCAAAACTGAATATCCTGGTGGCCACGCCCTGGCGTCGCCACCATAGC
GAACTCTGGAGAACTGAACA

Downstream 100 bases:

>100_bases
CACGCCTGAAACCGGCAAGCCGGCGGCTGGCACCAAGCCCGCTGCCCGCACCGAGGCCAAGGCGCCGCCCAAGAGCGCGC
GCCGCCGCTCGCGCGAGCTG

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MDHGFYPSNLDGEGLRIGIVQARFNEPVCAELLEACVAELEKLGVEGEDTLVVTVPGALEVPLALQKMAESGQFDALVAL
GAVVRGETYHFELVSNESGAGITRVGLDFNMPVANGILTVDTDEQAHARTREKGRDCARAAVEMANLVVALDSLRDQEDE
EDEDDDE

Sequences:

>Translated_167_residues
MDHGFYPSNLDGEGLRIGIVQARFNEPVCAELLEACVAELEKLGVEGEDTLVVTVPGALEVPLALQKMAESGQFDALVAL
GAVVRGETYHFELVSNESGAGITRVGLDFNMPVANGILTVDTDEQAHARTREKGRDCARAAVEMANLVVALDSLRDQEDE
EDEDDDE
>Mature_167_residues
MDHGFYPSNLDGEGLRIGIVQARFNEPVCAELLEACVAELEKLGVEGEDTLVVTVPGALEVPLALQKMAESGQFDALVAL
GAVVRGETYHFELVSNESGAGITRVGLDFNMPVANGILTVDTDEQAHARTREKGRDCARAAVEMANLVVALDSLRDQEDE
EDEDDDE

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786617, Length=143, Percent_Identity=41.958041958042, Blast_Score=105, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324429, Length=148, Percent_Identity=33.1081081081081, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002180 [H]

Pfam domain/function: PF00885 DMRL_synthase [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 17988; Mature: 17988

Theoretical pI: Translated: 3.89; Mature: 3.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHGFYPSNLDGEGLRIGIVQARFNEPVCAELLEACVAELEKLGVEGEDTLVVTVPGALE
CCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
VPLALQKMAESGQFDALVALGAVVRGETYHFELVSNESGAGITRVGLDFNMPVANGILTV
CCHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCEECCCCCCCEEEE
DTDEQAHARTREKGRDCARAAVEMANLVVALDSLRDQEDEEDEDDDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MDHGFYPSNLDGEGLRIGIVQARFNEPVCAELLEACVAELEKLGVEGEDTLVVTVPGALE
CCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
VPLALQKMAESGQFDALVALGAVVRGETYHFELVSNESGAGITRVGLDFNMPVANGILTV
CCHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCEECCCCCCCEEEE
DTDEQAHARTREKGRDCARAAVEMANLVVALDSLRDQEDEEDEDDDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA