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The map label for this gene is tolC [H]

Identifier: 73540453

GI number: 73540453

Start: 810542

End: 812035

Strand: Reverse

Name: tolC [H]

Synonym: Reut_A0749

Alternate gene names: 73540453

Gene position: 812035-810542 (Counterclockwise)

Preceding gene: 73540454

Following gene: 73540448

Centisome position: 21.33

GC content: 66.67

Gene sequence:

>1494_bases
ATGACGTTTGCGCCTTTCGTCGCGACCGGCAGGATGCGACTGGCAGTTGCGCTGTCCTTGTATGCGCTTCTGCAAGTGCC
GACGGCCTCGGCCGCCGACCTGCTGCAGGTCTACCGCGATGCCCAGTCCAACGACGCCCAGTTCGCGAGCGCGCGCTCGC
AACTGCTGGCCACGCGTGAAAAGCTGCCGCAGGGCCGGGCCGGACTGCTGCCGCTCGTCACGGGCACCGCTGGCGCCACG
CGGACCAGGCTTGACCAGACCGCGGCGCTGGCGCAGGGGCTGCCCAGCGCCTCGGGGGTTCGGTTCTTCAACAACAACAA
CTGGGCGCTCCAACTGAGCCAGCCGCTGTTCCGCTGGGACCGCTGGGAAACCTACAAGCAGGGCGAACTGGCCGCGATGG
CCGGCGAAGTCACGTTCCAGCAGGCGCAGCTCGATTTGATCACCCGGTCAGCGCAGGCCTACTTCGACGTGCTCACGGCG
CAGGACAATCTCTATCTGGCCGGCGCGCAGAAGAAGGCCATCGGCGAGCAACTTGCGCAGGCGAAGCGCAACTTCGAGGT
CGGCACCGCCACCATCACAGACGCCAACGATGCGCAGGCCCGCTACGACCTGGCCACGTCGACGGAGATCGCCGCGCAGA
GCGCACTGGAAGTGGCCCGAGCCAACCTGCAGCAGATCACCGGCAAGCCCGTGGATGAGCTGCTCGGACTACGGCCCGAG
GCGGATCTGCCGGCGCCAGTGCCTGCAGATTCCAATGCCTGGGCCGCGCAGGCCGAGGCCAGCAACCTTCAGGTTGGCCT
GGCCAACTACAACCTCGAAACCGCCCAGCGCGAAACCAACAAGGCGAAAGCCGCGCACCTGCCTTCGGTAGACCTGGTGG
CTTCCTACGGCTTCACCAACCAGACCGGCAGCGCCACGCAGACGATCTCGACGCACTACAACGCGTCGCAGATCGGCGTG
CAGCTGAGCATGCCGATCTTCGCGGGCGGCCAGATCCAGTCGCGCGTACGTGAAACGATCGCACTGGCCGACAAGGCCGC
CAGCGACCTCGAATTCGCGCGCCGCACGGCCGCGCAAAGCGCGCGCCAGAGCTATTCAGGCGTGTCCAACGGCCTCGCAC
AGGTCAAGGCGCTGGAAGCTGCCGAACGCTCGGCGCAGAGCGCGGTGGAATCGAACCAGCTCGGCTACGAGGTCGGCGTG
CGCATCAACATCGATGTGCTGAACGCCGAGGCGCAACTGTTCCAGACGCGCGCCAACCTGGCCAAGGCACGCTATGACAC
GATCATGAACGGCCTGCGGCTGAAGGCCTCGACAGGCGTGCTGCAGGAAAACGATATCGTCCAGATCAACACGCTGCTGA
CATCGGCACCGGGAGCGATGTATCAGTTGCCGCAGAAGGCGAAGATCGGCGCCGTGACGGCGCCGTCGCCGGCCCCGATG
TCTCGCCGCAACGGCCGGCGCGAAGCGCAGGCAACCGGCACGCCGCGCACCTGA

Upstream 100 bases:

>100_bases
TGAGAGGCCGAAGCCCCGCTTGGTTGCCGATCGCCCGCGAAGTCAAACCCAAACAAAAAATGACCGTTGGCCGCGCCTGT
GCCGGCAACCTGGAGATGTG

Downstream 100 bases:

>100_bases
AGAGAACGATCGACAAAACGAAATGGGCCGCAATGCGGCCTGTTTCGTTTGGGTGGCAGCCTAGTTCGCCCGAGTGCGCG
ACTCCACGCTAAGCAGCTGC

Product: Type I secretion outer membrane protein, TolC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTFAPFVATGRMRLAVALSLYALLQVPTASAADLLQVYRDAQSNDAQFASARSQLLATREKLPQGRAGLLPLVTGTAGAT
RTRLDQTAALAQGLPSASGVRFFNNNNWALQLSQPLFRWDRWETYKQGELAAMAGEVTFQQAQLDLITRSAQAYFDVLTA
QDNLYLAGAQKKAIGEQLAQAKRNFEVGTATITDANDAQARYDLATSTEIAAQSALEVARANLQQITGKPVDELLGLRPE
ADLPAPVPADSNAWAAQAEASNLQVGLANYNLETAQRETNKAKAAHLPSVDLVASYGFTNQTGSATQTISTHYNASQIGV
QLSMPIFAGGQIQSRVRETIALADKAASDLEFARRTAAQSARQSYSGVSNGLAQVKALEAAERSAQSAVESNQLGYEVGV
RINIDVLNAEAQLFQTRANLAKARYDTIMNGLRLKASTGVLQENDIVQINTLLTSAPGAMYQLPQKAKIGAVTAPSPAPM
SRRNGRREAQATGTPRT

Sequences:

>Translated_497_residues
MTFAPFVATGRMRLAVALSLYALLQVPTASAADLLQVYRDAQSNDAQFASARSQLLATREKLPQGRAGLLPLVTGTAGAT
RTRLDQTAALAQGLPSASGVRFFNNNNWALQLSQPLFRWDRWETYKQGELAAMAGEVTFQQAQLDLITRSAQAYFDVLTA
QDNLYLAGAQKKAIGEQLAQAKRNFEVGTATITDANDAQARYDLATSTEIAAQSALEVARANLQQITGKPVDELLGLRPE
ADLPAPVPADSNAWAAQAEASNLQVGLANYNLETAQRETNKAKAAHLPSVDLVASYGFTNQTGSATQTISTHYNASQIGV
QLSMPIFAGGQIQSRVRETIALADKAASDLEFARRTAAQSARQSYSGVSNGLAQVKALEAAERSAQSAVESNQLGYEVGV
RINIDVLNAEAQLFQTRANLAKARYDTIMNGLRLKASTGVLQENDIVQINTLLTSAPGAMYQLPQKAKIGAVTAPSPAPM
SRRNGRREAQATGTPRT
>Mature_496_residues
TFAPFVATGRMRLAVALSLYALLQVPTASAADLLQVYRDAQSNDAQFASARSQLLATREKLPQGRAGLLPLVTGTAGATR
TRLDQTAALAQGLPSASGVRFFNNNNWALQLSQPLFRWDRWETYKQGELAAMAGEVTFQQAQLDLITRSAQAYFDVLTAQ
DNLYLAGAQKKAIGEQLAQAKRNFEVGTATITDANDAQARYDLATSTEIAAQSALEVARANLQQITGKPVDELLGLRPEA
DLPAPVPADSNAWAAQAEASNLQVGLANYNLETAQRETNKAKAAHLPSVDLVASYGFTNQTGSATQTISTHYNASQIGVQ
LSMPIFAGGQIQSRVRETIALADKAASDLEFARRTAAQSARQSYSGVSNGLAQVKALEAAERSAQSAVESNQLGYEVGVR
INIDVLNAEAQLFQTRANLAKARYDTIMNGLRLKASTGVLQENDIVQINTLLTSAPGAMYQLPQKAKIGAVTAPSPAPMS
RRNGRREAQATGTPRT

Specific function: Required for proper expression of outer membrane protein genes such as ompF, nmpC, protein 2, hemolysin, colicin V, or colicin E1. May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=480, Percent_Identity=32.9166666666667, Blast_Score=220, Evalue=1e-58,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53140; Mature: 53009

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFAPFVATGRMRLAVALSLYALLQVPTASAADLLQVYRDAQSNDAQFASARSQLLATRE
CCCCCCHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KLPQGRAGLLPLVTGTAGATRTRLDQTAALAQGLPSASGVRFFNNNNWALQLSQPLFRWD
HCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCHHHHHH
RWETYKQGELAAMAGEVTFQQAQLDLITRSAQAYFDVLTAQDNLYLAGAQKKAIGEQLAQ
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHH
AKRNFEVGTATITDANDAQARYDLATSTEIAAQSALEVARANLQQITGKPVDELLGLRPE
HHHCCCCCEEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCC
ADLPAPVPADSNAWAAQAEASNLQVGLANYNLETAQRETNKAKAAHLPSVDLVASYGFTN
CCCCCCCCCCCCCCEECCCCCCEEEEEECCCCHHHHHHHCCHHHHCCCCHHHHEECCCCC
QTGSATQTISTHYNASQIGVQLSMPIFAGGQIQSRVRETIALADKAASDLEFARRTAAQS
CCCCCCEEEECCCCHHHCCEEEECCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARQSYSGVSNGLAQVKALEAAERSAQSAVESNQLGYEVGVRINIDVLNAEAQLFQTRANL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHHHHHHH
AKARYDTIMNGLRLKASTGVLQENDIVQINTLLTSAPGAMYQLPQKAKIGAVTAPSPAPM
HHHHHHHHHCCCEEEECCCCCCCCCEEEEEEHHHCCCCHHHHCCCCCCCCEEECCCCCCC
SRRNGRREAQATGTPRT
HHCCCCCCHHCCCCCCC
>Mature Secondary Structure 
TFAPFVATGRMRLAVALSLYALLQVPTASAADLLQVYRDAQSNDAQFASARSQLLATRE
CCCCCHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KLPQGRAGLLPLVTGTAGATRTRLDQTAALAQGLPSASGVRFFNNNNWALQLSQPLFRWD
HCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCHHHHHH
RWETYKQGELAAMAGEVTFQQAQLDLITRSAQAYFDVLTAQDNLYLAGAQKKAIGEQLAQ
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHH
AKRNFEVGTATITDANDAQARYDLATSTEIAAQSALEVARANLQQITGKPVDELLGLRPE
HHHCCCCCEEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCC
ADLPAPVPADSNAWAAQAEASNLQVGLANYNLETAQRETNKAKAAHLPSVDLVASYGFTN
CCCCCCCCCCCCCCEECCCCCCEEEEEECCCCHHHHHHHCCHHHHCCCCHHHHEECCCCC
QTGSATQTISTHYNASQIGVQLSMPIFAGGQIQSRVRETIALADKAASDLEFARRTAAQS
CCCCCCEEEECCCCHHHCCEEEECCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARQSYSGVSNGLAQVKALEAAERSAQSAVESNQLGYEVGVRINIDVLNAEAQLFQTRANL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHHHHHHH
AKARYDTIMNGLRLKASTGVLQENDIVQINTLLTSAPGAMYQLPQKAKIGAVTAPSPAPM
HHHHHHHHHCCCEEEECCCCCCCCCEEEEEEHHHCCCCHHHHCCCCCCCCEEECCCCCCC
SRRNGRREAQATGTPRT
HHCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6312426; 2216730; 9278503; 6303857; 9298646; 9629924; 10879525 [H]