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The map label for this gene is waaA [H]

Identifier: 73540451

GI number: 73540451

Start: 808236

End: 809525

Strand: Direct

Name: waaA [H]

Synonym: Reut_A0747

Alternate gene names: 73540451

Gene position: 808236-809525 (Clockwise)

Preceding gene: 73540450

Following gene: 73540452

Centisome position: 21.23

GC content: 68.91

Gene sequence:

>1290_bases
ATGCTACGCGTTCTGTACAGCTTGCTTTGGGTCGTCGTCCTGCCGCTGGCGCTGCTGCGGCTAGCATGGCGCGGTCGCAA
GGAGCCGGGATACGTTCAACATGTTGGCGAGCGTCTCGGCGCCTACGGACACCTGCCCGCGCAAGGTCCATGGCTGTGGG
TGCATGCGGTGTCCGTGGGCGAGACCCGCGCCGCACAGCCGCTGGTCGAGGCGCTGCTAGGCGCCTATCCGTCCCACCGG
CTGCTGCTCACGCACATGACGCCGACCGGCCGGCAGACCGGCGCGCAGCTTTTCGGCAAGGAGCCCCGCGTACTCCAGTG
CTATCTGCCCTATGACCTGCCGTGGCTGGTGCGTCGTTTCCTGCGGTATTTCCAGCCGGCGGCGGGCATGCTGATGGAAA
CCGAGGTCTGGCCGAATCTCGTACGCGGTGCGCGCCGTGCCGGCGTGCCGCTGTTCCTCGTCAATGCTCGGCTGTCGCCG
CGCAGCTACCGCCGCACGGTGCGTTTTGGACGCGCCGCAGCCGCCATGTACGCGGACTTTGCCGGCGTCCTGGCCCAGAC
CAGCGGTGACGCGGAGCGGTTCCAGGCGCTGGGCATTGATGCGGTGCAGATCACCGGCAACCTCAAATTCGACATGCAGC
CGGCGCCAGCCGGTGTCGAGCGCGGCCATCGCTTGCGTCAGGGTTTCGGCGGCCGTGCCGTGCTCGCTGCGGCCAGCACG
CGTGAAGGGGAGGAGCCGTTGCTGCTCGATGCGCTGTCTCGCTGGGCGCAGTTGGGTGGCCAGACGCCACGGCCTGCGAT
GCTGCTTGTGCCGCGACATCCGCAACGCTTTGACGAGGTGGCGAGCATGGCTGAGCGCGCGGGCTTCACGGTCCAGCGCC
GCAGCGAAATGGATATCGACAAGCTGGCTGGGCCCATCACCGCCGACATCGTGCTCGGCGATTCGATGGGCGAGATGGCG
ATGTACTTTGCCGCCTCGGACCTGGCCTTCGTCGGCGGCAGCCTGCTGCCGCTCGGCGGGCAGAACCTGATCGAGTCCTG
CGCAGTGGGCACTCCGGTGCTGGTCGGCCCGCATACGTTCAATTTTGCGCAGGCAACGGAAGATGCGATCGCGGCCGGCG
CATGCCGGCGCGTGACGAATGCCGACGAGCTGATGCAGGCTGCGGCCGGCCTGCTCGCCGATGCCGCGGCGCTGGCCGAC
ATGCGCGCGCATGCACGCACGTTTGCGGGCATGCACCGCGGTGCCACCGTGCGCACGCTGGCCGCGGTGGCGCCCGCGCT
GGGCGCCTGA

Upstream 100 bases:

>100_bases
ACTGGTCCGAACGCATCGCCAATGTGGGCGACGACGGCGTCGTGCCCACGGTCGATGAAGTCTGGCAGGCCACGTGCCGC
GTGCGCGGGACAGGAAACTG

Downstream 100 bases:

>100_bases
GCCGGCATCGCGCAATCCATGCCGCGCAGGGAATTCGCGGCAATGCCCCAGGCTCTAAGGGGCATCAACCTGGTGCGCCG
GGCAGTCAGTTGCCTGCCCG

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MLRVLYSLLWVVVLPLALLRLAWRGRKEPGYVQHVGERLGAYGHLPAQGPWLWVHAVSVGETRAAQPLVEALLGAYPSHR
LLLTHMTPTGRQTGAQLFGKEPRVLQCYLPYDLPWLVRRFLRYFQPAAGMLMETEVWPNLVRGARRAGVPLFLVNARLSP
RSYRRTVRFGRAAAAMYADFAGVLAQTSGDAERFQALGIDAVQITGNLKFDMQPAPAGVERGHRLRQGFGGRAVLAAAST
REGEEPLLLDALSRWAQLGGQTPRPAMLLVPRHPQRFDEVASMAERAGFTVQRRSEMDIDKLAGPITADIVLGDSMGEMA
MYFAASDLAFVGGSLLPLGGQNLIESCAVGTPVLVGPHTFNFAQATEDAIAAGACRRVTNADELMQAAAGLLADAAALAD
MRAHARTFAGMHRGATVRTLAAVAPALGA

Sequences:

>Translated_429_residues
MLRVLYSLLWVVVLPLALLRLAWRGRKEPGYVQHVGERLGAYGHLPAQGPWLWVHAVSVGETRAAQPLVEALLGAYPSHR
LLLTHMTPTGRQTGAQLFGKEPRVLQCYLPYDLPWLVRRFLRYFQPAAGMLMETEVWPNLVRGARRAGVPLFLVNARLSP
RSYRRTVRFGRAAAAMYADFAGVLAQTSGDAERFQALGIDAVQITGNLKFDMQPAPAGVERGHRLRQGFGGRAVLAAAST
REGEEPLLLDALSRWAQLGGQTPRPAMLLVPRHPQRFDEVASMAERAGFTVQRRSEMDIDKLAGPITADIVLGDSMGEMA
MYFAASDLAFVGGSLLPLGGQNLIESCAVGTPVLVGPHTFNFAQATEDAIAAGACRRVTNADELMQAAAGLLADAAALAD
MRAHARTFAGMHRGATVRTLAAVAPALGA
>Mature_429_residues
MLRVLYSLLWVVVLPLALLRLAWRGRKEPGYVQHVGERLGAYGHLPAQGPWLWVHAVSVGETRAAQPLVEALLGAYPSHR
LLLTHMTPTGRQTGAQLFGKEPRVLQCYLPYDLPWLVRRFLRYFQPAAGMLMETEVWPNLVRGARRAGVPLFLVNARLSP
RSYRRTVRFGRAAAAMYADFAGVLAQTSGDAERFQALGIDAVQITGNLKFDMQPAPAGVERGHRLRQGFGGRAVLAAAST
REGEEPLLLDALSRWAQLGGQTPRPAMLLVPRHPQRFDEVASMAERAGFTVQRRSEMDIDKLAGPITADIVLGDSMGEMA
MYFAASDLAFVGGSLLPLGGQNLIESCAVGTPVLVGPHTFNFAQATEDAIAAGACRRVTNADELMQAAAGLLADAAALAD
MRAHARTFAGMHRGATVRTLAAVAPALGA

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=430, Percent_Identity=38.8372093023256, Blast_Score=266, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 46315; Mature: 46315

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVLYSLLWVVVLPLALLRLAWRGRKEPGYVQHVGERLGAYGHLPAQGPWLWVHAVSVG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEECC
ETRAAQPLVEALLGAYPSHRLLLTHMTPTGRQTGAQLFGKEPRVLQCYLPYDLPWLVRRF
CHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHH
LRYFQPAAGMLMETEVWPNLVRGARRAGVPLFLVNARLSPRSYRRTVRFGRAAAAMYADF
HHHHCHHCCCEEEHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
AGVLAQTSGDAERFQALGIDAVQITGNLKFDMQPAPAGVERGHRLRQGFGGRAVLAAAST
HHHHHHCCCCHHHHHHHCCEEEEEECCEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCC
REGEEPLLLDALSRWAQLGGQTPRPAMLLVPRHPQRFDEVASMAERAGFTVQRRSEMDID
CCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEECCCCCCHH
KLAGPITADIVLGDSMGEMAMYFAASDLAFVGGSLLPLGGQNLIESCAVGTPVLVGPHTF
HHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEECCCCC
NFAQATEDAIAAGACRRVTNADELMQAAAGLLADAAALADMRAHARTFAGMHRGATVRTL
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AAVAPALGA
HHHHHHCCC
>Mature Secondary Structure
MLRVLYSLLWVVVLPLALLRLAWRGRKEPGYVQHVGERLGAYGHLPAQGPWLWVHAVSVG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEECC
ETRAAQPLVEALLGAYPSHRLLLTHMTPTGRQTGAQLFGKEPRVLQCYLPYDLPWLVRRF
CHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHH
LRYFQPAAGMLMETEVWPNLVRGARRAGVPLFLVNARLSPRSYRRTVRFGRAAAAMYADF
HHHHCHHCCCEEEHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
AGVLAQTSGDAERFQALGIDAVQITGNLKFDMQPAPAGVERGHRLRQGFGGRAVLAAAST
HHHHHHCCCCHHHHHHHCCEEEEEECCEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCC
REGEEPLLLDALSRWAQLGGQTPRPAMLLVPRHPQRFDEVASMAERAGFTVQRRSEMDID
CCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEECCCCCCHH
KLAGPITADIVLGDSMGEMAMYFAASDLAFVGGSLLPLGGQNLIESCAVGTPVLVGPHTF
HHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEECCCCC
NFAQATEDAIAAGACRRVTNADELMQAAAGLLADAAALADMRAHARTFAGMHRGATVRTL
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AAVAPALGA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]