Click here to switch to the map view.

The map label for this gene is wcaJ [H]

Identifier: 73540447

GI number: 73540447

Start: 803053

End: 804366

Strand: Reverse

Name: wcaJ [H]

Synonym: Reut_A0743

Alternate gene names: 73540447

Gene position: 804366-803053 (Counterclockwise)

Preceding gene: 73540448

Following gene: 73540446

Centisome position: 21.13

GC content: 64.69

Gene sequence:

>1314_bases
ATGCAGAAAACTGACAGCCTGCGCCGGCCCGCGGCCAGCCGCGCCACCGGCTCCGAAACCATCCGCCGCGCCAGCCGCAT
GCTGGCATGGGCCCTATTCGGATTGCTGTTGTTCGGCATCAACATCGCCCTGGCCGAACTGGCCCAGCGCGCCGGCTTCG
TGACCTACGTCTTCCATCGCACGCTGGTGTGGTCGATCGCGCCGTACCTGGCGGCTTTCGTCCTGCTGCACCGCTCGCTG
CACCTGCCGGCGATGGAAGGCAACAGCCTCGCGGGGCTGGCGGCGACGCTGCCCTTCGCGGCGTTGCTGTTCGTATTTGC
GGGTTTCCATATCGAATACTCGCGCGGCGCCTTCCTGCTGGCCTACTTCACCACGCTGGCCTGGATCTGGTTCGGCTACC
GGCATTTCGTGCGCAACTATGTACCGGTCTTCGGCTATGCCGATGCTGCCGCGCTCGCTCAATTGGAAGCCATGCTCGCC
ATGCCCGGCGCGGCCCCGGCCGGCCGCACCCGCTTCCAGCCCATCAACTCGCTGGCCGAGGCCGTGAACTGCGACGGCCT
GATGCTCGACCGCAACGCCGCGGCCGATCCCGAGCGTACCCGCGCCCTGGCCCAGTTAAAACTGAGCCATGTACGCATCT
ATTCGGTCGAGCGCGTCGGCGAGATGCTGACCGGCCGTGTGGGCCTGGCGCACATCGACGAGAATTTCCTCGACGATTAC
GCCGCGCATTACTTCTATGGCTACCTCAAGCGCGTGATCGACATCGGCACGGTAGTCTGCCTCGCTCCGCTTGCATTGCC
GCTGGGACTGATCGTCGCGGCGGCCATCCGCCTGGAATCGCCGGGGCCTGCACTCTTCCGCCAGGTCCGCGCCGGCCTGC
TGGGCAAACCCTTCACCATGCTCAAATTCCGCAGCATGGCTGTGGATAACTCAGGCAACGCGCAGTTTGCCGCGCGCCGG
GACCCGCGGGTGACGGGCGTCGGCCGGTTCATCCGCAAATACCGACTCGATGAAATCCCGCAGCTGTGGAACGTGCTGAT
CGGCGACATGAGCCTGATCGGCCCCCGCCCTGAACAGATTCCGATGGTCGAGGACTTCGAGCGCACCATCGCCTACTACC
CGTATCGCCATCTGGTGCGACCGGGTCTGTCCGGCTGGGCGCAGGTTCAGCAAGGGTATGTGGGCACGCACGAAGAAACC
GTGACCAAGCTCAGCTACGACCTTTACTACGTCAAGCATTGCTCGTTTGCGCTGGACCTGCTGATTGCCCTGAAGACGAT
GCAGACGATCCTGACCGGGTACGGTGCCCGGTGA

Upstream 100 bases:

>100_bases
CAACAGTTCGGCCTGACGCGCATGATCCAGGCCACCTGGGCCGTCTACCAGGACACGCTCGCCAGCGCCCCGCGCTACCA
AGCGTCCGCACGACAACGCC

Downstream 100 bases:

>100_bases
CGGTGCAAGCATCGACCGGCATGCCCGCGCAGCGGCCCATGCGGATCCTGCTGCTTTGTACCGGCCTCAAGATGGGTGGC
GCGGAACAGCAGATTGCCGC

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MQKTDSLRRPAASRATGSETIRRASRMLAWALFGLLLFGINIALAELAQRAGFVTYVFHRTLVWSIAPYLAAFVLLHRSL
HLPAMEGNSLAGLAATLPFAALLFVFAGFHIEYSRGAFLLAYFTTLAWIWFGYRHFVRNYVPVFGYADAAALAQLEAMLA
MPGAAPAGRTRFQPINSLAEAVNCDGLMLDRNAAADPERTRALAQLKLSHVRIYSVERVGEMLTGRVGLAHIDENFLDDY
AAHYFYGYLKRVIDIGTVVCLAPLALPLGLIVAAAIRLESPGPALFRQVRAGLLGKPFTMLKFRSMAVDNSGNAQFAARR
DPRVTGVGRFIRKYRLDEIPQLWNVLIGDMSLIGPRPEQIPMVEDFERTIAYYPYRHLVRPGLSGWAQVQQGYVGTHEET
VTKLSYDLYYVKHCSFALDLLIALKTMQTILTGYGAR

Sequences:

>Translated_437_residues
MQKTDSLRRPAASRATGSETIRRASRMLAWALFGLLLFGINIALAELAQRAGFVTYVFHRTLVWSIAPYLAAFVLLHRSL
HLPAMEGNSLAGLAATLPFAALLFVFAGFHIEYSRGAFLLAYFTTLAWIWFGYRHFVRNYVPVFGYADAAALAQLEAMLA
MPGAAPAGRTRFQPINSLAEAVNCDGLMLDRNAAADPERTRALAQLKLSHVRIYSVERVGEMLTGRVGLAHIDENFLDDY
AAHYFYGYLKRVIDIGTVVCLAPLALPLGLIVAAAIRLESPGPALFRQVRAGLLGKPFTMLKFRSMAVDNSGNAQFAARR
DPRVTGVGRFIRKYRLDEIPQLWNVLIGDMSLIGPRPEQIPMVEDFERTIAYYPYRHLVRPGLSGWAQVQQGYVGTHEET
VTKLSYDLYYVKHCSFALDLLIALKTMQTILTGYGAR
>Mature_437_residues
MQKTDSLRRPAASRATGSETIRRASRMLAWALFGLLLFGINIALAELAQRAGFVTYVFHRTLVWSIAPYLAAFVLLHRSL
HLPAMEGNSLAGLAATLPFAALLFVFAGFHIEYSRGAFLLAYFTTLAWIWFGYRHFVRNYVPVFGYADAAALAQLEAMLA
MPGAAPAGRTRFQPINSLAEAVNCDGLMLDRNAAADPERTRALAQLKLSHVRIYSVERVGEMLTGRVGLAHIDENFLDDY
AAHYFYGYLKRVIDIGTVVCLAPLALPLGLIVAAAIRLESPGPALFRQVRAGLLGKPFTMLKFRSMAVDNSGNAQFAARR
DPRVTGVGRFIRKYRLDEIPQLWNVLIGDMSLIGPRPEQIPMVEDFERTIAYYPYRHLVRPGLSGWAQVQQGYVGTHEET
VTKLSYDLYYVKHCSFALDLLIALKTMQTILTGYGAR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=182, Percent_Identity=41.7582417582418, Blast_Score=148, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 48628; Mature: 48628

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTDSLRRPAASRATGSETIRRASRMLAWALFGLLLFGINIALAELAQRAGFVTYVFHR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TLVWSIAPYLAAFVLLHRSLHLPAMEGNSLAGLAATLPFAALLFVFAGFHIEYSRGAFLL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHHH
AYFTTLAWIWFGYRHFVRNYVPVFGYADAAALAQLEAMLAMPGAAPAGRTRFQPINSLAE
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
AVNCDGLMLDRNAAADPERTRALAQLKLSHVRIYSVERVGEMLTGRVGLAHIDENFLDDY
HHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AAHYFYGYLKRVIDIGTVVCLAPLALPLGLIVAAAIRLESPGPALFRQVRAGLLGKPFTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHH
LKFRSMAVDNSGNAQFAARRDPRVTGVGRFIRKYRLDEIPQLWNVLIGDMSLIGPRPEQI
HHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PMVEDFERTIAYYPYRHLVRPGLSGWAQVQQGYVGTHEETVTKLSYDLYYVKHCSFALDL
CCHHHHHHHHHHCCHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LIALKTMQTILTGYGAR
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQKTDSLRRPAASRATGSETIRRASRMLAWALFGLLLFGINIALAELAQRAGFVTYVFHR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TLVWSIAPYLAAFVLLHRSLHLPAMEGNSLAGLAATLPFAALLFVFAGFHIEYSRGAFLL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHHH
AYFTTLAWIWFGYRHFVRNYVPVFGYADAAALAQLEAMLAMPGAAPAGRTRFQPINSLAE
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
AVNCDGLMLDRNAAADPERTRALAQLKLSHVRIYSVERVGEMLTGRVGLAHIDENFLDDY
HHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AAHYFYGYLKRVIDIGTVVCLAPLALPLGLIVAAAIRLESPGPALFRQVRAGLLGKPFTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHH
LKFRSMAVDNSGNAQFAARRDPRVTGVGRFIRKYRLDEIPQLWNVLIGDMSLIGPRPEQI
HHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PMVEDFERTIAYYPYRHLVRPGLSGWAQVQQGYVGTHEETVTKLSYDLYYVKHCSFALDL
CCHHHHHHHHHHCCHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LIALKTMQTILTGYGAR
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]