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The map label for this gene is btuB [H]

Identifier: 73540360

GI number: 73540360

Start: 702494

End: 704410

Strand: Direct

Name: btuB [H]

Synonym: Reut_A0654

Alternate gene names: 73540360

Gene position: 702494-704410 (Clockwise)

Preceding gene: 73540354

Following gene: 73540361

Centisome position: 18.45

GC content: 63.8

Gene sequence:

>1917_bases
ATGCGTTCATCGCTTTACAGGTCGTACCGTCCGGCACTGCCGACGGTGCGTCCGTCCGTAGCTGCCGTCCTGGCTGCCGT
GTCTTCCCTTGTCCTGAATCCCGCCTTGGCCCAGACCGAGACTGGCAGTGGGTCTCAACCGGTTGCCGGTACCGCCGCTG
CGACACAACTGCCGCCGGTGGTGGTCACCTCGACCCGCACGGCACAGCCGTTGACCGAGGCGCTGCCGCATACCACGGTG
GTGACGCAGGAGGATATCGTCAACTCGCAAGCGCCGGACCTGCGCACGCTGCTGCGCAACCAGGCCGGTGTGGAGCTGAC
CGCCAACGGCGGCATGGGCTCGAACACGTCGATATTCATGCGCGGCGCCAACTCCAACCAGACGCTGATTCTGATCGACG
GCGCGCGCGTCTCGTCGGTCAGCAGCGGCACCGCTCAGCTGGCCAACATCCTGCCTGACCAGATCGACCACATCGAAGTG
GTGCGGGGCAACGTGTCGGCGCTGTACGGCTCGGATGCGATCGGCGGCGTGGTGCAGATTTTCACCAAGAGCGGCGCGGG
CCAGGCCCCGGCGGCGAACGCGCAGGTCGAGTACGGCAGCAACAATACGCGCCAGGGGACGGTTGGCTATGGCGGCCAGA
TCGGCGACACCTCGTTCAACGTGACGGGTTCTGCGTTCAAGACCGACGGATTTTCGTCGATCAACACGAAGCAGGCGCCG
CGCGCTAACCCGAACGACAATCCGTACGAGAACCAGAGCGTATCGGCGCAAGTCAAGCACCGCTTCACGCCGGACTGGGA
TGCGGGCATGGCCGGCTACTACTCGAAGAGCAAGCTGTCGTATGACAGCAGCTTCGGCCGCCCGACCGACGACAACCGCA
GCAACAGCGAGCTGTACACGGTGTCGGCATTCGTCAACGGCAAGGTGCTGCCGGACTGGGCCACGCATTTCAAGCTTGCA
CAGAGCGAGGACCGCAGCGAAAACTTCCTCAACGGTGCCAACAACGGCACCTTCAACACGCGTAACCGCCAGTTCAACTG
GCAGAACGAATACGCGCTGGCGCCGACCCACACGCTGCTGTTCGGTACCGACTACCTGCAGCAAGAGCTCGATTCGAACT
CGTACGGCGCGCCGTCGCGCAATGTGTTCTCGGTGTTCGGTGGCTATGACGGGCGCTTTGGCAAGCACCAGATCCAGCTC
AACGGGCGCAACGACCACTATTCGGACTTCGGCAACCAGGGCAGCTTCTTTGCGGGCTATGGCTACAACGTGACCGACGC
GCTCAAGCTGATCGCCAATGTCAGCAATGCTTTCCGTGCGCCAACGTTCAACGAGCTGTATTTCCCGGGCTTCGGCAATC
CGAACCTGAACCCCGAGCGCGCCAAGTCGGCGGAAGTCGGCGTGCAGTACAACACGCCGCAAACCGGCCTGCTGCGCGTG
ACGACGTTCGAGACCCGCTACGACGACCTGATCGTCAACGTCACGCAGCCCAACGGCCTGCTGGCGCCGACCAACGTCAA
CAAGGCGAAGGTGCAGGGCATCGAGGCGTCCTGGCGTGCGTCGCTGTGGGGTACCGACGTGGGCGCGAGCGTGACGTTCC
AGAACCCGGTGGACGAACAGACCGACACGCAGCTGCTGCGCCGCGCGCGCCGCCATGCCGCGTTCGACGTGGGCCGCAAT
TTCGGCAACTGGCGTTTTGGCGGCGAATGGCTGCTGTCCTCGTCGCGCCTGGATACGGGCTCGCGCACGCTGGGCGGATA
CGGTATCGTCAACCTCAACGCGCGCTACAACATCGACAAGCAATGGTTCGTCGCTGCCCGGGTCGAGAACCTGTTCGACA
AGAACTACCAGCTGGTCTACCCCTACAACACGCAGGGCCGGGCCGGCTATATCACGCTGGGCTGGCGCCAGAAATGA

Upstream 100 bases:

>100_bases
GATACCGGCCTGAACGCGGGCTGCCGCCCCTGCGCGGCACGCCTCATCGCCACATGGCCCGCGGGGGAACGGGCCCGGCC
GTCCTATCCATTCCTGCATC

Downstream 100 bases:

>100_bases
CGGGCGGTGTGGCGCGCCATGTCTGACTTCCGGCGCGCCCTCGCGGTGTGGCTGGCGCTCGCTGCGGTCGCGGTGGCGGT
GTTCCTGCTGTCGCTGGCGT

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 638; Mature: 638

Protein sequence:

>638_residues
MRSSLYRSYRPALPTVRPSVAAVLAAVSSLVLNPALAQTETGSGSQPVAGTAAATQLPPVVVTSTRTAQPLTEALPHTTV
VTQEDIVNSQAPDLRTLLRNQAGVELTANGGMGSNTSIFMRGANSNQTLILIDGARVSSVSSGTAQLANILPDQIDHIEV
VRGNVSALYGSDAIGGVVQIFTKSGAGQAPAANAQVEYGSNNTRQGTVGYGGQIGDTSFNVTGSAFKTDGFSSINTKQAP
RANPNDNPYENQSVSAQVKHRFTPDWDAGMAGYYSKSKLSYDSSFGRPTDDNRSNSELYTVSAFVNGKVLPDWATHFKLA
QSEDRSENFLNGANNGTFNTRNRQFNWQNEYALAPTHTLLFGTDYLQQELDSNSYGAPSRNVFSVFGGYDGRFGKHQIQL
NGRNDHYSDFGNQGSFFAGYGYNVTDALKLIANVSNAFRAPTFNELYFPGFGNPNLNPERAKSAEVGVQYNTPQTGLLRV
TTFETRYDDLIVNVTQPNGLLAPTNVNKAKVQGIEASWRASLWGTDVGASVTFQNPVDEQTDTQLLRRARRHAAFDVGRN
FGNWRFGGEWLLSSSRLDTGSRTLGGYGIVNLNARYNIDKQWFVAARVENLFDKNYQLVYPYNTQGRAGYITLGWRQK

Sequences:

>Translated_638_residues
MRSSLYRSYRPALPTVRPSVAAVLAAVSSLVLNPALAQTETGSGSQPVAGTAAATQLPPVVVTSTRTAQPLTEALPHTTV
VTQEDIVNSQAPDLRTLLRNQAGVELTANGGMGSNTSIFMRGANSNQTLILIDGARVSSVSSGTAQLANILPDQIDHIEV
VRGNVSALYGSDAIGGVVQIFTKSGAGQAPAANAQVEYGSNNTRQGTVGYGGQIGDTSFNVTGSAFKTDGFSSINTKQAP
RANPNDNPYENQSVSAQVKHRFTPDWDAGMAGYYSKSKLSYDSSFGRPTDDNRSNSELYTVSAFVNGKVLPDWATHFKLA
QSEDRSENFLNGANNGTFNTRNRQFNWQNEYALAPTHTLLFGTDYLQQELDSNSYGAPSRNVFSVFGGYDGRFGKHQIQL
NGRNDHYSDFGNQGSFFAGYGYNVTDALKLIANVSNAFRAPTFNELYFPGFGNPNLNPERAKSAEVGVQYNTPQTGLLRV
TTFETRYDDLIVNVTQPNGLLAPTNVNKAKVQGIEASWRASLWGTDVGASVTFQNPVDEQTDTQLLRRARRHAAFDVGRN
FGNWRFGGEWLLSSSRLDTGSRTLGGYGIVNLNARYNIDKQWFVAARVENLFDKNYQLVYPYNTQGRAGYITLGWRQK
>Mature_638_residues
MRSSLYRSYRPALPTVRPSVAAVLAAVSSLVLNPALAQTETGSGSQPVAGTAAATQLPPVVVTSTRTAQPLTEALPHTTV
VTQEDIVNSQAPDLRTLLRNQAGVELTANGGMGSNTSIFMRGANSNQTLILIDGARVSSVSSGTAQLANILPDQIDHIEV
VRGNVSALYGSDAIGGVVQIFTKSGAGQAPAANAQVEYGSNNTRQGTVGYGGQIGDTSFNVTGSAFKTDGFSSINTKQAP
RANPNDNPYENQSVSAQVKHRFTPDWDAGMAGYYSKSKLSYDSSFGRPTDDNRSNSELYTVSAFVNGKVLPDWATHFKLA
QSEDRSENFLNGANNGTFNTRNRQFNWQNEYALAPTHTLLFGTDYLQQELDSNSYGAPSRNVFSVFGGYDGRFGKHQIQL
NGRNDHYSDFGNQGSFFAGYGYNVTDALKLIANVSNAFRAPTFNELYFPGFGNPNLNPERAKSAEVGVQYNTPQTGLLRV
TTFETRYDDLIVNVTQPNGLLAPTNVNKAKVQGIEASWRASLWGTDVGASVTFQNPVDEQTDTQLLRRARRHAAFDVGRN
FGNWRFGGEWLLSSSRLDTGSRTLGGYGIVNLNARYNIDKQWFVAARVENLFDKNYQLVYPYNTQGRAGYITLGWRQK

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=601, Percent_Identity=27.6206322795341, Blast_Score=195, Evalue=9e-51,
Organism=Escherichia coli, GI1788478, Length=695, Percent_Identity=26.6187050359712, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1786798, Length=704, Percent_Identity=24.5738636363636, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 69384; Mature: 69384

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSLYRSYRPALPTVRPSVAAVLAAVSSLVLNPALAQTETGSGSQPVAGTAAATQLPPV
CCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHCCCCCE
VVTSTRTAQPLTEALPHTTVVTQEDIVNSQAPDLRTLLRNQAGVELTANGGMGSNTSIFM
EEECCCCCCHHHHHCCCCEEEEHHHHHCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEE
RGANSNQTLILIDGARVSSVSSGTAQLANILPDQIDHIEVVRGNVSALYGSDAIGGVVQI
EECCCCCEEEEEECCCCCCCCCCHHHHHHHCHHHCCEEEEEECCEEEEECCCHHCCEEEE
FTKSGAGQAPAANAQVEYGSNNTRQGTVGYGGQIGDTSFNVTGSAFKTDGFSSINTKQAP
EECCCCCCCCCCCCEEEECCCCCCCCCCCCCCEECCCEEEECCCEEECCCCCCCCCCCCC
RANPNDNPYENQSVSAQVKHRFTPDWDAGMAGYYSKSKLSYDSSFGRPTDDNRSNSELYT
CCCCCCCCCCCCCCEEHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
VSAFVNGKVLPDWATHFKLAQSEDRSENFLNGANNGTFNTRNRQFNWQNEYALAPTHTLL
EEEEECCEECCCHHHHHEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCEEEE
FGTDYLQQELDSNSYGAPSRNVFSVFGGYDGRFGKHQIQLNGRNDHYSDFGNQGSFFAGY
ECCHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEC
GYNVTDALKLIANVSNAFRAPTFNELYFPGFGNPNLNPERAKSAEVGVQYNTPQTGLLRV
CCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHCCCCCCCEEECCCCCCEEEE
TTFETRYDDLIVNVTQPNGLLAPTNVNKAKVQGIEASWRASLWGTDVGASVTFQNPVDEQ
EEECCCCCEEEEEEECCCCEECCCCCCHHHEECCCCCCEEEECCCCCCCEEEECCCCCCH
TDTQLLRRARRHAAFDVGRNFGNWRFGGEWLLSSSRLDTGSRTLGGYGIVNLNARYNIDK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCEECCCH
QWFVAARVENLFDKNYQLVYPYNTQGRAGYITLGWRQK
HEEHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCC
>Mature Secondary Structure
MRSSLYRSYRPALPTVRPSVAAVLAAVSSLVLNPALAQTETGSGSQPVAGTAAATQLPPV
CCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHCCCCCE
VVTSTRTAQPLTEALPHTTVVTQEDIVNSQAPDLRTLLRNQAGVELTANGGMGSNTSIFM
EEECCCCCCHHHHHCCCCEEEEHHHHHCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEE
RGANSNQTLILIDGARVSSVSSGTAQLANILPDQIDHIEVVRGNVSALYGSDAIGGVVQI
EECCCCCEEEEEECCCCCCCCCCHHHHHHHCHHHCCEEEEEECCEEEEECCCHHCCEEEE
FTKSGAGQAPAANAQVEYGSNNTRQGTVGYGGQIGDTSFNVTGSAFKTDGFSSINTKQAP
EECCCCCCCCCCCCEEEECCCCCCCCCCCCCCEECCCEEEECCCEEECCCCCCCCCCCCC
RANPNDNPYENQSVSAQVKHRFTPDWDAGMAGYYSKSKLSYDSSFGRPTDDNRSNSELYT
CCCCCCCCCCCCCCEEHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
VSAFVNGKVLPDWATHFKLAQSEDRSENFLNGANNGTFNTRNRQFNWQNEYALAPTHTLL
EEEEECCEECCCHHHHHEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCEEEE
FGTDYLQQELDSNSYGAPSRNVFSVFGGYDGRFGKHQIQLNGRNDHYSDFGNQGSFFAGY
ECCHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEC
GYNVTDALKLIANVSNAFRAPTFNELYFPGFGNPNLNPERAKSAEVGVQYNTPQTGLLRV
CCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHCCCCCCCEEECCCCCCEEEE
TTFETRYDDLIVNVTQPNGLLAPTNVNKAKVQGIEASWRASLWGTDVGASVTFQNPVDEQ
EEECCCCCEEEEEEECCCCEECCCCCCHHHEECCCCCCEEEECCCCCCCEEEECCCCCCH
TDTQLLRRARRHAAFDVGRNFGNWRFGGEWLLSSSRLDTGSRTLGGYGIVNLNARYNIDK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCEECCCH
QWFVAARVENLFDKNYQLVYPYNTQGRAGYITLGWRQK
HEEHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA