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The map label for this gene is ilvD1

Identifier: 73540345

GI number: 73540345

Start: 686135

End: 687808

Strand: Reverse

Name: ilvD1

Synonym: Reut_A0639

Alternate gene names: 73540345

Gene position: 687808-686135 (Counterclockwise)

Preceding gene: 73540348

Following gene: 73540344

Centisome position: 18.07

GC content: 63.38

Gene sequence:

>1674_bases
ATGGCATTCAACAAACGCTCCCGCAACATCACGCAAGGTGTGGCGCGTTCCCCGAACCGCTCGATGTACTACGCCCTGGG
CTACCAGAAGGAAGACTTCGACAAGCCCATGGTCGGCATCGCCAACGGCCACTCGACGATCACGCCGTGCAACGCCGGCC
TGCAGCGCCTGGCCGACGCCGCGATCGACGCCGTGAAGGCCTCGGACGCCAATCCGCAGGTATTCGGCACGCCGACGATC
TCCGACGGCATGTCGATGGGCACCGAGGGCATGAAGTACTCGCTGATCTCGCGCGAAGTCATTGCCGACTGCATCGAGAC
CGCCGCCCAGGGCCAGTGGATGGACGGCGTGGTGGTGATCGGCGGCTGCGACAAGAACATGCCGGGCGGCATGATCGCGC
TCGCGCGCACCAATGTGCCGGGCATCTACGTCTATGGCGGCACCATCAAGCCCGGCAACTGGAAGGGCAAGGACCTGACC
ATCGTGTCGTCGTTCGAGGCCGTGGGCGAATTCACCGCCGGCCGCATGAGCGAAGAAGATTTCGAGGGCGTGGAAAGGAA
CGCTTGTCCGTCGACCGGATCGTGCGGCGGCATGTACACCGCCAATACGATGAGCTCGTCGTTCGAGGCGCTTGGGATGT
CGCTGCTGTATTCGTCGACGATGGCCAACCCCGATCAGGAAAAGGTCGATAGCGCTGCCGAATCGGCGCGCGTGCTGGTC
GAGGCGATCAAGAAGGACATCAAGCCGCGCGACATCATCACGCGCAAGTCGATCGAAAACGCCGTCGCGCTGATCATGGC
CACCGGTGGCTCGACCAACGCGGTGCTGCACTACCTGGCCATTGCGCACTCGGCCGAAGTCGAATGGACCATCGACGATT
TCGAGCGCATCCGTCGCAAGGTGCCTGTGATCTGCAACCTGAAGCCGTCGGGCCAGTACGTGGCCACCGACCTGCACAAG
GCGGGCGGCATCCCGCAGGTCATGAAGATCCTGCTGAAGGCCGGACTGCTGCATGGCGACTGCCTGACCATCACAGGCCG
CACGCTGGCTGAAGAGCTGGAACACGTGCCGGACCAGCCGCGGGCCGACCAGGACGTGATCATGCCAATCGAGAAGGCGC
TCTATGCGGAGGGGCATCTGGCGATCCTGAAGGGCAATCTCGCCGAGGAAGGCGCGGTGGCCAAGATCACCGGCCTGAAG
AATCCGGTCATCACCGGGCCGGCGCGGGTGTTCGAGGATGAACAGAGCGCGATGGAGGCGATCCTGGCCGACAAGATCAA
TGCGGGGGATGTGCTGGTGCTGCGCTATCTTGGGCCCAAGGGCGGGCCCGGGATGCCGGAAATGCTGGCGCCTACGTCAG
CCATTATTGGCAAGGGGTTGGGAGAGTCGGTGGGCTTCATCACCGATGGCCGGTTCTCCGGGGGCACCTGGGGGATGGTC
GTCGGCCATGTCGCGCCGGAAGCGTATGTAGGCGGCAATATTGCGCTCGTGCGGGAAGGGGATTCGATCACGATCGATGC
GCACAAGCTGGTGTTGCAGGTGAATGTGTCGGATGAGGAGTTGGCTCGCCGCCGGGCTGAGTGGAAACAACCTGTTGCGC
GTTATACACGGGGGGTATTGGCCAAGTTTGCTCGACTGGCGTCGACGGCCAGTAAGGGGGCGGTGACGGATTGA

Upstream 100 bases:

>100_bases
ATAAAGGTCAAATAATATATTTGACACTCCAAAACGCAAGTCGCATTCTTTGCACAAACCCCCGAGCCTCAGCCGAAGAA
CATTCCCCGGAGACACGACC

Downstream 100 bases:

>100_bases
AGGAGAGGTTCGGGCTGGGGTATCGCTCTTGGCATGCGCCGCTGTTTCGCCGGCGTAGCCGGCGACCTACTTCTTGTCCA
AGCGACAAGAAGTAGGCAAG

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD 1

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MAFNKRSRNITQGVARSPNRSMYYALGYQKEDFDKPMVGIANGHSTITPCNAGLQRLADAAIDAVKASDANPQVFGTPTI
SDGMSMGTEGMKYSLISREVIADCIETAAQGQWMDGVVVIGGCDKNMPGGMIALARTNVPGIYVYGGTIKPGNWKGKDLT
IVSSFEAVGEFTAGRMSEEDFEGVERNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
EAIKKDIKPRDIITRKSIENAVALIMATGGSTNAVLHYLAIAHSAEVEWTIDDFERIRRKVPVICNLKPSGQYVATDLHK
AGGIPQVMKILLKAGLLHGDCLTITGRTLAEELEHVPDQPRADQDVIMPIEKALYAEGHLAILKGNLAEEGAVAKITGLK
NPVITGPARVFEDEQSAMEAILADKINAGDVLVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMV
VGHVAPEAYVGGNIALVREGDSITIDAHKLVLQVNVSDEELARRRAEWKQPVARYTRGVLAKFARLASTASKGAVTD

Sequences:

>Translated_557_residues
MAFNKRSRNITQGVARSPNRSMYYALGYQKEDFDKPMVGIANGHSTITPCNAGLQRLADAAIDAVKASDANPQVFGTPTI
SDGMSMGTEGMKYSLISREVIADCIETAAQGQWMDGVVVIGGCDKNMPGGMIALARTNVPGIYVYGGTIKPGNWKGKDLT
IVSSFEAVGEFTAGRMSEEDFEGVERNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
EAIKKDIKPRDIITRKSIENAVALIMATGGSTNAVLHYLAIAHSAEVEWTIDDFERIRRKVPVICNLKPSGQYVATDLHK
AGGIPQVMKILLKAGLLHGDCLTITGRTLAEELEHVPDQPRADQDVIMPIEKALYAEGHLAILKGNLAEEGAVAKITGLK
NPVITGPARVFEDEQSAMEAILADKINAGDVLVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMV
VGHVAPEAYVGGNIALVREGDSITIDAHKLVLQVNVSDEELARRRAEWKQPVARYTRGVLAKFARLASTASKGAVTD
>Mature_556_residues
AFNKRSRNITQGVARSPNRSMYYALGYQKEDFDKPMVGIANGHSTITPCNAGLQRLADAAIDAVKASDANPQVFGTPTIS
DGMSMGTEGMKYSLISREVIADCIETAAQGQWMDGVVVIGGCDKNMPGGMIALARTNVPGIYVYGGTIKPGNWKGKDLTI
VSSFEAVGEFTAGRMSEEDFEGVERNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLVE
AIKKDIKPRDIITRKSIENAVALIMATGGSTNAVLHYLAIAHSAEVEWTIDDFERIRRKVPVICNLKPSGQYVATDLHKA
GGIPQVMKILLKAGLLHGDCLTITGRTLAEELEHVPDQPRADQDVIMPIEKALYAEGHLAILKGNLAEEGAVAKITGLKN
PVITGPARVFEDEQSAMEAILADKINAGDVLVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMVV
GHVAPEAYVGGNIALVREGDSITIDAHKLVLQVNVSDEELARRRAEWKQPVARYTRGVLAKFARLASTASKGAVTD

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=606, Percent_Identity=37.7887788778878, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI2367371, Length=502, Percent_Identity=36.0557768924303, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1786464, Length=476, Percent_Identity=36.9747899159664, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI1788157, Length=469, Percent_Identity=31.9829424307036, Blast_Score=199, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6322476, Length=563, Percent_Identity=46.3587921847247, Blast_Score=505, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD1_CUPPJ (Q475B2)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294865.1
- ProteinModelPortal:   Q475B2
- SMR:   Q475B2
- GeneID:   3610628
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0639
- NMPDR:   fig|264198.3.peg.1128
- HOGENOM:   HBG671001
- OMA:   NMPGAMI
- ProtClustDB:   PRK00911
- BioCyc:   REUT264198:REUT_A0639-MONOMER
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 59280; Mature: 59148

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFNKRSRNITQGVARSPNRSMYYALGYQKEDFDKPMVGIANGHSTITPCNAGLQRLADA
CCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
AIDAVKASDANPQVFGTPTISDGMSMGTEGMKYSLISREVIADCIETAAQGQWMDGVVVI
HHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEE
GGCDKNMPGGMIALARTNVPGIYVYGGTIKPGNWKGKDLTIVSSFEAVGEFTAGRMSEED
ECCCCCCCCCEEEEEECCCCEEEEECCEECCCCCCCCEEEEEECHHHHHHHHCCCCCCHH
FEGVERNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
HHCCHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EAIKKDIKPRDIITRKSIENAVALIMATGGSTNAVLHYLAIAHSAEVEWTIDDFERIRRK
HHHHHCCCCHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHC
VPVICNLKPSGQYVATDLHKAGGIPQVMKILLKAGLLHGDCLTITGRTLAEELEHVPDQP
CCEEEEECCCCCEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCC
RADQDVIMPIEKALYAEGHLAILKGNLAEEGAVAKITGLKNPVITGPARVFEDEQSAMEA
CCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCEECCHHHHCCHHHHHHH
ILADKINAGDVLVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMV
HHHCCCCCCCEEEEEEECCCCCCCCCHHHCCHHHHHHCCCCCCCCEEECCCCCCCCCEEE
VGHVAPEAYVGGNIALVREGDSITIDAHKLVLQVNVSDEELARRRAEWKQPVARYTRGVL
EECCCCCCEECCCEEEEECCCEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
AKFARLASTASKGAVTD
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AFNKRSRNITQGVARSPNRSMYYALGYQKEDFDKPMVGIANGHSTITPCNAGLQRLADA
CCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
AIDAVKASDANPQVFGTPTISDGMSMGTEGMKYSLISREVIADCIETAAQGQWMDGVVVI
HHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEE
GGCDKNMPGGMIALARTNVPGIYVYGGTIKPGNWKGKDLTIVSSFEAVGEFTAGRMSEED
ECCCCCCCCCEEEEEECCCCEEEEECCEECCCCCCCCEEEEEECHHHHHHHHCCCCCCHH
FEGVERNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
HHCCHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EAIKKDIKPRDIITRKSIENAVALIMATGGSTNAVLHYLAIAHSAEVEWTIDDFERIRRK
HHHHHCCCCHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHC
VPVICNLKPSGQYVATDLHKAGGIPQVMKILLKAGLLHGDCLTITGRTLAEELEHVPDQP
CCEEEEECCCCCEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCC
RADQDVIMPIEKALYAEGHLAILKGNLAEEGAVAKITGLKNPVITGPARVFEDEQSAMEA
CCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCEECCHHHHCCHHHHHHH
ILADKINAGDVLVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMV
HHHCCCCCCCEEEEEEECCCCCCCCCHHHCCHHHHHHCCCCCCCCEEECCCCCCCCCEEE
VGHVAPEAYVGGNIALVREGDSITIDAHKLVLQVNVSDEELARRRAEWKQPVARYTRGVL
EECCCCCCEECCCEEEEECCCEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
AKFARLASTASKGAVTD
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA