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The map label for this gene is lpxK

Identifier: 73540299

GI number: 73540299

Start: 643635

End: 644735

Strand: Reverse

Name: lpxK

Synonym: Reut_A0593

Alternate gene names: 73540299

Gene position: 644735-643635 (Counterclockwise)

Preceding gene: 73540300

Following gene: 73540298

Centisome position: 16.94

GC content: 67.94

Gene sequence:

>1101_bases
ATGTCCGCTCCGCGCCATGACCTCGCCGATTTCGTGACCGCCCAGTGGCAACGCCGGGGTTGGTTCGCCTGGCTGATGCT
GCCGTTCTCGCTGCTGTTCGGGCTGATCGCGCGCGTGCGCCGCTACGGTTACCTGCATGGCTGGTTCAAGTCGACGCGGC
TGCCGATGCCGGTGATCGTCGTGGGTAATGTGACCGTTGGCGGTACCGGCAAGACGCCGGCAGTGATCGCGCTGGCGCAG
GCACTGACCGAGGCCGGCCTGCGTCCGGGGGTGGTGTCGCGCGGTTACGGCGTTACGCTCAAGCATCCGCGCCGAGTCAA
GCCGACGTCAAAGGCGTCGGATGTCGGCGACGAACCGCTGCTGATTGCGCGCGCCGCCGACGTGCCGGTCTGGGTATTCC
CCGACCGCGCGCTGTGCGCGCAGACCATGCTGGTGTCGCACCCGGGCGTCAACGTGCTGCTGCTGGACGACGGCCTGCAG
CACTACCGCCTGCAGCGCGACTTCGAGATCGTCATGTTCGATTCGCGCATGGGCGGCAACGGCCTGATGCTGCCCGCCGG
TCCGCTGCGCGAGCCGCTTTCACGCCCGCGCGACGCCACGCTGATCAATGACCCGAACTTCCGCGCCACGCCCGAGCGGC
CCGATGTCTATGGCATGCGGCTGGATCTTGATGAGGCCTGGCAGCTCAACGATCCGACCATGGCACGCCCGGTCAGCGCG
TTTGCCGGGCGCCGCGTGCTGGCCGCAGCCGGTATCGGCAACCCCGAACGGTTCTTCGCGAGCCTGCGCGGTGCGGGCCT
GAGTCCGAAGACCCTGCCGCTGCCCGATCACTACGATTTCGCCGAGGACCCGTTCGCCGGCAACCCTGACGCGCTCGACG
CCGACGTCATCCTGATCACCGAGAAGGATGCCGTAAAATGCGAGCGATTCGACGACCCGCGCATCTGGGTCGTCCCCACC
ACGCCCGTGATCGATGCGGGCCTGATCGACAAGATCCGACGCGCCGTGCTGGCGCGCGTACCGGCCGCAAAGAGCACGCC
CGCATCGGGCGGCGCCACGGGCCTCAACAAGGAACACCAAGATGGACAACCGGCTGCTTGA

Upstream 100 bases:

>100_bases
CCGGCAGGGCGCCGGGGTTGCCCCGTGCGCAGGGCAATGCCCATAATGTGCGCGTCCGGCCGGCCGCCAGGCCAGCCAAC
CACCCTTCCCCTGCCCTTCC

Downstream 100 bases:

>100_bases
AATCCTGGTCTGCCCGCTTTGCAAGGGCAAGCTGGAATACGACCGCGCCGCGCAGGAACTGATCTGCCACGCGGACAAGC
TCGCCTACCCGATCCGCGAC

Product: tetraacyldisaccharide 4'-kinase

Products: NA

Alternate protein names: Lipid A 4'-kinase

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MSAPRHDLADFVTAQWQRRGWFAWLMLPFSLLFGLIARVRRYGYLHGWFKSTRLPMPVIVVGNVTVGGTGKTPAVIALAQ
ALTEAGLRPGVVSRGYGVTLKHPRRVKPTSKASDVGDEPLLIARAADVPVWVFPDRALCAQTMLVSHPGVNVLLLDDGLQ
HYRLQRDFEIVMFDSRMGGNGLMLPAGPLREPLSRPRDATLINDPNFRATPERPDVYGMRLDLDEAWQLNDPTMARPVSA
FAGRRVLAAAGIGNPERFFASLRGAGLSPKTLPLPDHYDFAEDPFAGNPDALDADVILITEKDAVKCERFDDPRIWVVPT
TPVIDAGLIDKIRRAVLARVPAAKSTPASGGATGLNKEHQDGQPAA

Sequences:

>Translated_366_residues
MSAPRHDLADFVTAQWQRRGWFAWLMLPFSLLFGLIARVRRYGYLHGWFKSTRLPMPVIVVGNVTVGGTGKTPAVIALAQ
ALTEAGLRPGVVSRGYGVTLKHPRRVKPTSKASDVGDEPLLIARAADVPVWVFPDRALCAQTMLVSHPGVNVLLLDDGLQ
HYRLQRDFEIVMFDSRMGGNGLMLPAGPLREPLSRPRDATLINDPNFRATPERPDVYGMRLDLDEAWQLNDPTMARPVSA
FAGRRVLAAAGIGNPERFFASLRGAGLSPKTLPLPDHYDFAEDPFAGNPDALDADVILITEKDAVKCERFDDPRIWVVPT
TPVIDAGLIDKIRRAVLARVPAAKSTPASGGATGLNKEHQDGQPAA
>Mature_365_residues
SAPRHDLADFVTAQWQRRGWFAWLMLPFSLLFGLIARVRRYGYLHGWFKSTRLPMPVIVVGNVTVGGTGKTPAVIALAQA
LTEAGLRPGVVSRGYGVTLKHPRRVKPTSKASDVGDEPLLIARAADVPVWVFPDRALCAQTMLVSHPGVNVLLLDDGLQH
YRLQRDFEIVMFDSRMGGNGLMLPAGPLREPLSRPRDATLINDPNFRATPERPDVYGMRLDLDEAWQLNDPTMARPVSAF
AGRRVLAAAGIGNPERFFASLRGAGLSPKTLPLPDHYDFAEDPFAGNPDALDADVILITEKDAVKCERFDDPRIWVVPTT
PVIDAGLIDKIRRAVLARVPAAKSTPASGGATGLNKEHQDGQPAA

Specific function: Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1- P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA)

COG id: COG1663

COG function: function code M; Tetraacyldisaccharide-1-P 4'-kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lpxK family

Homologues:

Organism=Escherichia coli, GI1787144, Length=301, Percent_Identity=40.8637873754153, Blast_Score=194, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXK_CUPPJ (Q475F8)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294819.1
- GeneID:   3610777
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0593
- NMPDR:   fig|264198.3.peg.1105
- HOGENOM:   HBG663914
- OMA:   GNIHAGG
- ProtClustDB:   PRK01906
- BioCyc:   REUT264198:REUT_A0593-MONOMER
- HAMAP:   MF_00409
- InterPro:   IPR003758
- TIGRFAMs:   TIGR00682

Pfam domain/function: PF02606 LpxK

EC number: =2.7.1.130

Molecular weight: Translated: 39942; Mature: 39811

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPRHDLADFVTAQWQRRGWFAWLMLPFSLLFGLIARVRRYGYLHGWFKSTRLPMPVIV
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEE
VGNVTVGGTGKTPAVIALAQALTEAGLRPGVVSRGYGVTLKHPRRVKPTSKASDVGDEPL
EECEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCE
LIARAADVPVWVFPDRALCAQTMLVSHPGVNVLLLDDGLQHYRLQRDFEIVMFDSRMGGN
EEEEECCCEEEECCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCEEEEEEECCCCCC
GLMLPAGPLREPLSRPRDATLINDPNFRATPERPDVYGMRLDLDEAWQLNDPTMARPVSA
EEEECCCHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHH
FAGRRVLAAAGIGNPERFFASLRGAGLSPKTLPLPDHYDFAEDPFAGNPDALDADVILIT
HHCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
EKDAVKCERFDDPRIWVVPTTPVIDAGLIDKIRRAVLARVPAAKSTPASGGATGLNKEHQ
CCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
DGQPAA
CCCCCC
>Mature Secondary Structure 
SAPRHDLADFVTAQWQRRGWFAWLMLPFSLLFGLIARVRRYGYLHGWFKSTRLPMPVIV
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEE
VGNVTVGGTGKTPAVIALAQALTEAGLRPGVVSRGYGVTLKHPRRVKPTSKASDVGDEPL
EECEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCE
LIARAADVPVWVFPDRALCAQTMLVSHPGVNVLLLDDGLQHYRLQRDFEIVMFDSRMGGN
EEEEECCCEEEECCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCEEEEEEECCCCCC
GLMLPAGPLREPLSRPRDATLINDPNFRATPERPDVYGMRLDLDEAWQLNDPTMARPVSA
EEEECCCHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHH
FAGRRVLAAAGIGNPERFFASLRGAGLSPKTLPLPDHYDFAEDPFAGNPDALDADVILIT
HHCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
EKDAVKCERFDDPRIWVVPTTPVIDAGLIDKIRRAVLARVPAAKSTPASGGATGLNKEHQ
CCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
DGQPAA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA