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The map label for this gene is kdsB

Identifier: 73540297

GI number: 73540297

Start: 642631

End: 643440

Strand: Reverse

Name: kdsB

Synonym: Reut_A0591

Alternate gene names: 73540297

Gene position: 643440-642631 (Counterclockwise)

Preceding gene: 73540298

Following gene: 73540296

Centisome position: 16.9

GC content: 69.26

Gene sequence:

>810_bases
ATGAGCATCCCGGCCTTCACTGTCGTCATTCCGGCGCGGCTGGCTTCCACGCGGCTGCCGAACAAGCCGCTGGCCGATAT
CGGTGGTCACCCGATGATCGTGCGCGTGGCCGAGCGTGCGCACGCGTCGTCAGCGCAGCGCACCGTGGTGGCCACCGATG
CGCCGGCTGTCGCCGAAGCGTGCGCCGCGCATGGCATCGACGCGGTACTGACGCGTGCTGACCACCCGTCGGGCACGGAC
CGGCTGGCCGAAGTCGCAGCACAGCTTGCGCTGCCTGACGATGCCATCGTCGTCAACGTACAGGGCGACGAGCCGCTGAT
CGACCCGCAGCTGATCGACGACGTGGCAATGCACCTTGCTCACCACGCCGATTGTGCGATCGCCACTGCCGCGCATGCGC
TGCACGACGTGGCTGAAGTCTTCAACCCGAACGTCGTCAAGGTCGTTTGCGATGCCGGCGGACGTGCGCTGTACTTCTCG
CGCGCACCGATTCCGTGGTCGCGCGACGACTGGGCCGGCGTGCCGGCGGTGCCCGCCTCGGCCGCGCAGGTGCCGCTGCC
GGCCATGCCGGTGTTGCGCCATATTGGCCTGTACGCCTACCGTGCCGGCTTCCTGCGCCGCTTCCCTACCATGGCGGCGG
CGCCGCTAGAGCAGACCGAAGCCCTGGAGCAACTGCGCGCCATGTGGCATGGCGAGCGCATTGCGGTGCTGGAAACGGCG
GCCGCACCGGCCCCCGGTGTCGACACCGCCGCGGATCTCGAACGCGTGCGCGCGCTGTGGGCGCAAGGTATGGCTCAGGA
AGGTCCCTGA

Upstream 100 bases:

>100_bases
CGCGACGGCATTCCCGTCATGCTGGCCGACGAGGCCCGGCAATCGGTGCCAGGGCGCGTGATCGACCCGGCCGCGCCGCG
GACGGAGTGAGGCGCGCATC

Downstream 100 bases:

>100_bases
CCACTTTGCCACGCGGCGGAGTGCTTCGCGCCGCCTTTGCGTTGGGGTTCATACTGATGCAACCATGGAATCAGCCGAAT
TCGGGCCGGATTCGTTGCGC

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSIPAFTVVIPARLASTRLPNKPLADIGGHPMIVRVAERAHASSAQRTVVATDAPAVAEACAAHGIDAVLTRADHPSGTD
RLAEVAAQLALPDDAIVVNVQGDEPLIDPQLIDDVAMHLAHHADCAIATAAHALHDVAEVFNPNVVKVVCDAGGRALYFS
RAPIPWSRDDWAGVPAVPASAAQVPLPAMPVLRHIGLYAYRAGFLRRFPTMAAAPLEQTEALEQLRAMWHGERIAVLETA
AAPAPGVDTAADLERVRALWAQGMAQEGP

Sequences:

>Translated_269_residues
MSIPAFTVVIPARLASTRLPNKPLADIGGHPMIVRVAERAHASSAQRTVVATDAPAVAEACAAHGIDAVLTRADHPSGTD
RLAEVAAQLALPDDAIVVNVQGDEPLIDPQLIDDVAMHLAHHADCAIATAAHALHDVAEVFNPNVVKVVCDAGGRALYFS
RAPIPWSRDDWAGVPAVPASAAQVPLPAMPVLRHIGLYAYRAGFLRRFPTMAAAPLEQTEALEQLRAMWHGERIAVLETA
AAPAPGVDTAADLERVRALWAQGMAQEGP
>Mature_268_residues
SIPAFTVVIPARLASTRLPNKPLADIGGHPMIVRVAERAHASSAQRTVVATDAPAVAEACAAHGIDAVLTRADHPSGTDR
LAEVAAQLALPDDAIVVNVQGDEPLIDPQLIDDVAMHLAHHADCAIATAAHALHDVAEVFNPNVVKVVCDAGGRALYFSR
APIPWSRDDWAGVPAVPASAAQVPLPAMPVLRHIGLYAYRAGFLRRFPTMAAAPLEQTEALEQLRAMWHGERIAVLETAA
APAPGVDTAADLERVRALWAQGMAQEGP

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=255, Percent_Identity=53.7254901960784, Blast_Score=254, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_CUPPJ (Q475G0)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294817.1
- ProteinModelPortal:   Q475G0
- SMR:   Q475G0
- GeneID:   3610775
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0591
- NMPDR:   fig|264198.3.peg.1103
- HOGENOM:   HBG637773
- OMA:   FSRAPLP
- ProtClustDB:   PRK05450
- BioCyc:   REUT264198:REUT_A0591-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 28341; Mature: 28209

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPAFTVVIPARLASTRLPNKPLADIGGHPMIVRVAERAHASSAQRTVVATDAPAVAEA
CCCCCEEEEEHHHHHHCCCCCCCHHHCCCCCEEEEEHHHHHCCCCCCEEEECCCHHHHHH
CAAHGIDAVLTRADHPSGTDRLAEVAAQLALPDDAIVVNVQGDEPLIDPQLIDDVAMHLA
HHHCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
HHADCAIATAAHALHDVAEVFNPNVVKVVCDAGGRALYFSRAPIPWSRDDWAGVPAVPAS
HHCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCC
AAQVPLPAMPVLRHIGLYAYRAGFLRRFPTMAAAPLEQTEALEQLRAMWHGERIAVLETA
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHCCCEEEEEEEC
AAPAPGVDTAADLERVRALWAQGMAQEGP
CCCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SIPAFTVVIPARLASTRLPNKPLADIGGHPMIVRVAERAHASSAQRTVVATDAPAVAEA
CCCCEEEEEHHHHHHCCCCCCCHHHCCCCCEEEEEHHHHHCCCCCCEEEECCCHHHHHH
CAAHGIDAVLTRADHPSGTDRLAEVAAQLALPDDAIVVNVQGDEPLIDPQLIDDVAMHLA
HHHCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
HHADCAIATAAHALHDVAEVFNPNVVKVVCDAGGRALYFSRAPIPWSRDDWAGVPAVPAS
HHCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCC
AAQVPLPAMPVLRHIGLYAYRAGFLRRFPTMAAAPLEQTEALEQLRAMWHGERIAVLETA
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHCCCEEEEEEEC
AAPAPGVDTAADLERVRALWAQGMAQEGP
CCCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA