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The map label for this gene is glyS [H]

Identifier: 73540213

GI number: 73540213

Start: 550007

End: 552139

Strand: Reverse

Name: glyS [H]

Synonym: Reut_A0507

Alternate gene names: 73540213

Gene position: 552139-550007 (Counterclockwise)

Preceding gene: 73540214

Following gene: 73540212

Centisome position: 14.51

GC content: 67.65

Gene sequence:

>2133_bases
ATGTCGCAACCCATGACCGACTCGCTGCTGATCGAACTGTTCACCGAAGAGCTGCCCCCGAAGGCCCTGGCGCGCCTGGG
CGACGCCTTCGCGCAGGGCCTGTTCGCCGGCCTGCGCGAGCGCGATCTGCTTGAAGCCGATGCCGCCGTCACGCCGTTTG
CTTCGCCGCGCCGCCTCGCCGCGCTGGTGTCCGGCGTGCGCCGCAGCGCGCCCGATCGCGAGCAGCGCGAGAAGGTCCTG
CCGCTGACCGTGGCGCTGGGCGCCAACGGCGAGCCCACCGCGCCGCTGGCCAAGAAGCTTGCCGCGCTGGCGAAGTCCGT
CGGCGTGGCCGAGATCGACTGGCAGACGCTGGAACGTGCCTCGGATGGCAAGGCTGAGGCCTTCTTCTACCGCTACACCG
CCAAGGGTGCCGAACTGGCCGCCGGCCTGCAGGCCGCGCTGGACGACACCATCGGCAAGCTGCCGATCCCGAAGGTGATG
AGTTACCAGCGCCCAGGCGGCGAAACCGTGCACTTCGTGCGCCCCGCGCACCGGCTGATCGCGCTGTTCGGCAGCGAGAT
CGTGCCCGTTACCCTGCTCGGCCTGCCAGCCAGCAACCTGACGCAGGGCCATCGCTTCCTGTCGCACGGCACGATCGAGA
TCGCGCACGCGACGCAGTACGCCGACACCCTGCTCGAGCAGGGCAAAGTCATCGCAAACTACGCGCAGCGCCGTGAATCG
ATCCGCGAGCAACTGCTGAAGGCTGCGGGCGCCGACCAGGTGGTCATGCCCGAAGCGCTGCTCGACGAAGTGAATTCGCT
GGTGGAATGGCCGGTGGTCTACGCCTGCCACTTCGAGGAAGCGTTCCTGGCTGTGCCGCAGGAATGCCTGATCCTGACCA
TGCAGACGAACCAAAAGTACTTTGCGCTGACCGATGCCGAAGGCCACCTGCGCAACCGCTTCCTGATCGTCTCGAACCTC
GCCACGGAAACGCCGCAGTCGATCATCACCGGCAACGAGCGCGTGGTGCGGCCGCGCCTGGCCGATGCCAAGTTCTTCTT
CGACCAGGACCGCAAGAAGACGCTGGCCTCGCGCGTGCCGCAGCTTGCTAGCGTGGTCTATCACAACAAGATCGGCACGC
AGCTCGATCGCGTACAGCGCCTGCAGCAGATCGCCGGCCACATCGCCGACGCGATGAATGCGTCCGGCTTCGCCGTCGAC
AAGGCCGCCGCGATGCGCGGCGCCGAACTGGCCAAGGCCGACCTGCTGACCGACATGGTCGGCGAATTCCCCGAACTGCA
AGGCACCATGGGCACGTACTACGCGCGCCATGACGACGAATCCGAGGACGTCGCGCTGGCCTGCTCCGAGCACTACCGTC
CGCGCTTTGCCGGTGACGTGCTGCCCGAAGGCGCCGTCAGCACTGCCGTGGCGCTCGCCGACAAACTCGAGACGCTGGTC
GGCATCTGGGGCATCGGCCTGCAGCCCACCGGCGAAAAAGACCCGTTTGCGCTGCGCCGCCACGCGCTGGGCATCCTGCG
CATGCTGATCGAGAAGCCGCTGGCCCTGTCGGTGGACACGCTGCTTGCGCTGACCCAGCAGGCCTTTGCCGGCGTGCCGG
CCGTCAAGCCGGCACCCGACGCGATTGCCGATTTCCTCTATGACCGCGTGCGGGGCTACCTGAAGGACAAGGGCTACACC
ACCAATGAAGTCGAGGCCGTGGTCAGCCTGCGCCCGCAGCAGTTGCATGACATTCTCGGCCGCCTGGAAGCCGTGCGCAC
GTTCGCCGCGCTGCCTGAAGCCGAGGCGCTGGCCGCCGCCAACAAGCGCATCACGAACATCCTGAAGAAGAACGCCGAAG
CCGCGCAGGACATCGCTGCGGTCAATCCGGCGCTGTTCCAGGAAGACGCCGAACGTGCACTGCACGGCGCCATCGAGCGC
GTGCGCCCGGACGCGGAAGCAGCCTTTGCGCGCGGCGACTTCACCAGCGCGTTGCGCGACCTGGCCCAGCTGCGCGAAGC
CGTCGACGGCTTCTTCGACAACGTGATGGTGATGGCCGAGGACGCGCAGCTGCGCGCGAACCGACTGGCGCTGCTGGCCT
CGCTGCACGCGCTGGCCAACCGCGTGGCGGACATCTCGAAGCTGGCGGCCTGA

Upstream 100 bases:

>100_bases
TCCGGCAACAGAACGGCCTCTGGCTGCTCTCCGGGGTAGAACCTAATCTTGAGCCGCGCTGCTGATCGCAGCGCGAGCCA
CAACCTGTTGGAAACGATTC

Downstream 100 bases:

>100_bases
TCGCCACGCCACCACGCCAACCGGGAGCCAGACATGCCACAGACACCGCCCAAATTCGTCGTGCTTGATCGCGACGGGGT
CATCAACCTTGACAGCGACC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MSQPMTDSLLIELFTEELPPKALARLGDAFAQGLFAGLRERDLLEADAAVTPFASPRRLAALVSGVRRSAPDREQREKVL
PLTVALGANGEPTAPLAKKLAALAKSVGVAEIDWQTLERASDGKAEAFFYRYTAKGAELAAGLQAALDDTIGKLPIPKVM
SYQRPGGETVHFVRPAHRLIALFGSEIVPVTLLGLPASNLTQGHRFLSHGTIEIAHATQYADTLLEQGKVIANYAQRRES
IREQLLKAAGADQVVMPEALLDEVNSLVEWPVVYACHFEEAFLAVPQECLILTMQTNQKYFALTDAEGHLRNRFLIVSNL
ATETPQSIITGNERVVRPRLADAKFFFDQDRKKTLASRVPQLASVVYHNKIGTQLDRVQRLQQIAGHIADAMNASGFAVD
KAAAMRGAELAKADLLTDMVGEFPELQGTMGTYYARHDDESEDVALACSEHYRPRFAGDVLPEGAVSTAVALADKLETLV
GIWGIGLQPTGEKDPFALRRHALGILRMLIEKPLALSVDTLLALTQQAFAGVPAVKPAPDAIADFLYDRVRGYLKDKGYT
TNEVEAVVSLRPQQLHDILGRLEAVRTFAALPEAEALAAANKRITNILKKNAEAAQDIAAVNPALFQEDAERALHGAIER
VRPDAEAAFARGDFTSALRDLAQLREAVDGFFDNVMVMAEDAQLRANRLALLASLHALANRVADISKLAA

Sequences:

>Translated_710_residues
MSQPMTDSLLIELFTEELPPKALARLGDAFAQGLFAGLRERDLLEADAAVTPFASPRRLAALVSGVRRSAPDREQREKVL
PLTVALGANGEPTAPLAKKLAALAKSVGVAEIDWQTLERASDGKAEAFFYRYTAKGAELAAGLQAALDDTIGKLPIPKVM
SYQRPGGETVHFVRPAHRLIALFGSEIVPVTLLGLPASNLTQGHRFLSHGTIEIAHATQYADTLLEQGKVIANYAQRRES
IREQLLKAAGADQVVMPEALLDEVNSLVEWPVVYACHFEEAFLAVPQECLILTMQTNQKYFALTDAEGHLRNRFLIVSNL
ATETPQSIITGNERVVRPRLADAKFFFDQDRKKTLASRVPQLASVVYHNKIGTQLDRVQRLQQIAGHIADAMNASGFAVD
KAAAMRGAELAKADLLTDMVGEFPELQGTMGTYYARHDDESEDVALACSEHYRPRFAGDVLPEGAVSTAVALADKLETLV
GIWGIGLQPTGEKDPFALRRHALGILRMLIEKPLALSVDTLLALTQQAFAGVPAVKPAPDAIADFLYDRVRGYLKDKGYT
TNEVEAVVSLRPQQLHDILGRLEAVRTFAALPEAEALAAANKRITNILKKNAEAAQDIAAVNPALFQEDAERALHGAIER
VRPDAEAAFARGDFTSALRDLAQLREAVDGFFDNVMVMAEDAQLRANRLALLASLHALANRVADISKLAA
>Mature_709_residues
SQPMTDSLLIELFTEELPPKALARLGDAFAQGLFAGLRERDLLEADAAVTPFASPRRLAALVSGVRRSAPDREQREKVLP
LTVALGANGEPTAPLAKKLAALAKSVGVAEIDWQTLERASDGKAEAFFYRYTAKGAELAAGLQAALDDTIGKLPIPKVMS
YQRPGGETVHFVRPAHRLIALFGSEIVPVTLLGLPASNLTQGHRFLSHGTIEIAHATQYADTLLEQGKVIANYAQRRESI
REQLLKAAGADQVVMPEALLDEVNSLVEWPVVYACHFEEAFLAVPQECLILTMQTNQKYFALTDAEGHLRNRFLIVSNLA
TETPQSIITGNERVVRPRLADAKFFFDQDRKKTLASRVPQLASVVYHNKIGTQLDRVQRLQQIAGHIADAMNASGFAVDK
AAAMRGAELAKADLLTDMVGEFPELQGTMGTYYARHDDESEDVALACSEHYRPRFAGDVLPEGAVSTAVALADKLETLVG
IWGIGLQPTGEKDPFALRRHALGILRMLIEKPLALSVDTLLALTQQAFAGVPAVKPAPDAIADFLYDRVRGYLKDKGYTT
NEVEAVVSLRPQQLHDILGRLEAVRTFAALPEAEALAAANKRITNILKKNAEAAQDIAAVNPALFQEDAERALHGAIERV
RPDAEAAFARGDFTSALRDLAQLREAVDGFFDNVMVMAEDAQLRANRLALLASLHALANRVADISKLAA

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=704, Percent_Identity=43.6079545454545, Blast_Score=536, Evalue=1e-153,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 77153; Mature: 77022

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPMTDSLLIELFTEELPPKALARLGDAFAQGLFAGLRERDLLEADAAVTPFASPRRLA
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
ALVSGVRRSAPDREQREKVLPLTVALGANGEPTAPLAKKLAALAKSVGVAEIDWQTLERA
HHHHHHHHCCCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHC
SDGKAEAFFYRYTAKGAELAAGLQAALDDTIGKLPIPKVMSYQRPGGETVHFVRPAHRLI
CCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEECHHHHHH
ALFGSEIVPVTLLGLPASNLTQGHRFLSHGTIEIAHATQYADTLLEQGKVIANYAQRRES
HHHCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHH
IREQLLKAAGADQVVMPEALLDEVNSLVEWPVVYACHFEEAFLAVPQECLILTMQTNQKY
HHHHHHHHCCCCCEECCHHHHHHHHHHHHCCHHEEEEHHHHHHHCCHHHEEEEEECCCEE
FALTDAEGHLRNRFLIVSNLATETPQSIITGNERVVRPRLADAKFFFDQDRKKTLASRVP
EEEECCCCHHHCCEEEEECCCCCCCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHH
QLASVVYHNKIGTQLDRVQRLQQIAGHIADAMNASGFAVDKAAAMRGAELAKADLLTDMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GEFPELQGTMGTYYARHDDESEDVALACSEHYRPRFAGDVLPEGAVSTAVALADKLETLV
HCCHHHCCCHHHHEECCCCCCCCCEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GIWGIGLQPTGEKDPFALRRHALGILRMLIEKPLALSVDTLLALTQQAFAGVPAVKPAPD
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
AIADFLYDRVRGYLKDKGYTTNEVEAVVSLRPQQLHDILGRLEAVRTFAALPEAEALAAA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
NKRITNILKKNAEAAQDIAAVNPALFQEDAERALHGAIERVRPDAEAAFARGDFTSALRD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHH
LAQLREAVDGFFDNVMVMAEDAQLRANRLALLASLHALANRVADISKLAA
HHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQPMTDSLLIELFTEELPPKALARLGDAFAQGLFAGLRERDLLEADAAVTPFASPRRLA
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
ALVSGVRRSAPDREQREKVLPLTVALGANGEPTAPLAKKLAALAKSVGVAEIDWQTLERA
HHHHHHHHCCCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHC
SDGKAEAFFYRYTAKGAELAAGLQAALDDTIGKLPIPKVMSYQRPGGETVHFVRPAHRLI
CCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEECHHHHHH
ALFGSEIVPVTLLGLPASNLTQGHRFLSHGTIEIAHATQYADTLLEQGKVIANYAQRRES
HHHCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHH
IREQLLKAAGADQVVMPEALLDEVNSLVEWPVVYACHFEEAFLAVPQECLILTMQTNQKY
HHHHHHHHCCCCCEECCHHHHHHHHHHHHCCHHEEEEHHHHHHHCCHHHEEEEEECCCEE
FALTDAEGHLRNRFLIVSNLATETPQSIITGNERVVRPRLADAKFFFDQDRKKTLASRVP
EEEECCCCHHHCCEEEEECCCCCCCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHH
QLASVVYHNKIGTQLDRVQRLQQIAGHIADAMNASGFAVDKAAAMRGAELAKADLLTDMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GEFPELQGTMGTYYARHDDESEDVALACSEHYRPRFAGDVLPEGAVSTAVALADKLETLV
HCCHHHCCCHHHHEECCCCCCCCCEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GIWGIGLQPTGEKDPFALRRHALGILRMLIEKPLALSVDTLLALTQQAFAGVPAVKPAPD
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
AIADFLYDRVRGYLKDKGYTTNEVEAVVSLRPQQLHDILGRLEAVRTFAALPEAEALAAA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
NKRITNILKKNAEAAQDIAAVNPALFQEDAERALHGAIERVRPDAEAAFARGDFTSALRD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHH
LAQLREAVDGFFDNVMVMAEDAQLRANRLALLASLHALANRVADISKLAA
HHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA