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The map label for this gene is purH [H]

Identifier: 73540193

GI number: 73540193

Start: 529871

End: 531442

Strand: Reverse

Name: purH [H]

Synonym: Reut_A0487

Alternate gene names: 73540193

Gene position: 531442-529871 (Counterclockwise)

Preceding gene: 73540194

Following gene: 73540192

Centisome position: 13.96

GC content: 65.33

Gene sequence:

>1572_bases
ATGATCAAGCAAGCTCTCCTCTCTGTTTCCGACAAGACCGGCATCGTCGAATTCGCGCGCGAACTGAACGCGCTCGGCGT
CACGCTGCTTTCCACCGGCGGCACCGCCAAGCTGCTGGCCGACAGCGGCCTGCCCGTCACGGAAGTGGCCGACTACACCG
GCTTCCCCGAAATGCTCGACGGCCGCGTGAAGACGCTGCATCCGAAGGTGCACGGCGGCATCCTCGCGCGCCGCGACCTG
CCCGAGCACATGGCCGCACTGGCCGAGCACGACATCCCGACGATCGACTTGCTGGTGGTGAACCTGTACCCGTTCCAGCA
GACCGTGGCAAAGGATGACTGCACGCTGCCCGACGCGATCGAGAACATCGACATCGGCGGCCCGACCATGCTGCGCTCGG
CTGCCAAGAACCACCGCGACGTGACCGTGATCGTCGATCCGGTCGACTACGCCGTGGTGCTGGACGAAATGCGCGCCAAT
GGCAACAAGGTTGGCTACGACACCAACTTCCGCCTCGCGACCAAGGTGTTCGCGCACACCGCGCAATACGACGGCGCGAT
CACCAACTACCTGACCAGCCTCGGTGCCGACAAGTCGCACCAGGCCCGCAGCGCCTACCCGCAGACGCTGAACCTGGCCT
TCGAGAAGGTCCAGGAAATGCGCTACGGCGAAAACCCGCACCAGTCGGCCGCGTTCTACCGCGACCTGAAGGCCGTGGAT
GGCGCACTGGCCAACTACGTGCAGCTGCAGGGCAAGGAACTGTCGTACAACAACATCGCCGACGCCGATGCGGCCTGGGA
ATGCGTGAAGTCCTTCGCCGTGACCACGCCCGCCTGCGTGATCATCAAGCACGCCAATCCGTGCGGCGTGGCTGTGGGCG
CCAATGCCCTCGAAGCCTACGACAAGGCTTTCAAGACCGACTCCACCTCGGCCTTCGGCGGCATCATCGCGTTCAACGTC
GAACTCGACGAAACCGCGGCCCAGGCCGTGGCGAAGCAGTTCGTCGAAGTGCTGATCGCCCCGTCGTTCAGCGCTGCCGC
GCGCGCCGTATTCGCATCCAAGCAGAACGTGCGCCTGCTCGAAATCCCGCTGGGCAAGGGCATCAACCAGTATGACCTCA
AGCGCGTGGGCGGTGGCCTGCTGGTGCAGAGCCCCGACGCCAAGAACGTGCAGCCGACCGAACTGCGCGTGGTCACGCGC
CGTCATCCGACGCCGAAGGAAATGGACGATCTGATGTTCGCCTGGCGCGTCGCCAAGTTCGTCAAGTCCAACGCCATCGT
ATTCTGCGGCGGCGGCATGACGCTGGGCGTCGGCGCAGGCCAGATGAGCCGCGTGGATTCGGCCCGCATCGCCAGCATCA
AGGCGCAGAACGCGGGCCTGACGCTGGCGGGTTCGGCCGTGGCATCGGACGCCTTCTTCCCGTTCCGTGATGGCCTGGAC
GTCGTGGTAGACGCCGGCGCAACCTGTGTCATCCAGCCGGGCGGTTCGATGCGCGACGACGAAGTGATCGCCGCCGCCGA
CGATCGCGGCATCGCCATGGTCCTGACCGGCACCCGCCATTTCCGCCACTAA

Upstream 100 bases:

>100_bases
CAGTCGCTGGCCGCCGCCTACCTCGGCATCAATCGCAATACGCTGCGCAAGAAGTTGCAGCTGCACGGTTTACTTTGAAT
TTGAAGCGTTTCGCACAGCC

Downstream 100 bases:

>100_bases
GCAAGGCAAAGAAATAGAAAGCTCCGGGGCGCGGACCTATCCGCGCCCCGGAGCTTTTTTTCTTTTTCTCAGCGCGGCCC
AGAGGCACAGTGCCCGAACC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MIKQALLSVSDKTGIVEFARELNALGVTLLSTGGTAKLLADSGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMAALAEHDIPTIDLLVVNLYPFQQTVAKDDCTLPDAIENIDIGGPTMLRSAAKNHRDVTVIVDPVDYAVVLDEMRAN
GNKVGYDTNFRLATKVFAHTAQYDGAITNYLTSLGADKSHQARSAYPQTLNLAFEKVQEMRYGENPHQSAAFYRDLKAVD
GALANYVQLQGKELSYNNIADADAAWECVKSFAVTTPACVIIKHANPCGVAVGANALEAYDKAFKTDSTSAFGGIIAFNV
ELDETAAQAVAKQFVEVLIAPSFSAAARAVFASKQNVRLLEIPLGKGINQYDLKRVGGGLLVQSPDAKNVQPTELRVVTR
RHPTPKEMDDLMFAWRVAKFVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLD
VVVDAGATCVIQPGGSMRDDEVIAAADDRGIAMVLTGTRHFRH

Sequences:

>Translated_523_residues
MIKQALLSVSDKTGIVEFARELNALGVTLLSTGGTAKLLADSGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMAALAEHDIPTIDLLVVNLYPFQQTVAKDDCTLPDAIENIDIGGPTMLRSAAKNHRDVTVIVDPVDYAVVLDEMRAN
GNKVGYDTNFRLATKVFAHTAQYDGAITNYLTSLGADKSHQARSAYPQTLNLAFEKVQEMRYGENPHQSAAFYRDLKAVD
GALANYVQLQGKELSYNNIADADAAWECVKSFAVTTPACVIIKHANPCGVAVGANALEAYDKAFKTDSTSAFGGIIAFNV
ELDETAAQAVAKQFVEVLIAPSFSAAARAVFASKQNVRLLEIPLGKGINQYDLKRVGGGLLVQSPDAKNVQPTELRVVTR
RHPTPKEMDDLMFAWRVAKFVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLD
VVVDAGATCVIQPGGSMRDDEVIAAADDRGIAMVLTGTRHFRH
>Mature_523_residues
MIKQALLSVSDKTGIVEFARELNALGVTLLSTGGTAKLLADSGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMAALAEHDIPTIDLLVVNLYPFQQTVAKDDCTLPDAIENIDIGGPTMLRSAAKNHRDVTVIVDPVDYAVVLDEMRAN
GNKVGYDTNFRLATKVFAHTAQYDGAITNYLTSLGADKSHQARSAYPQTLNLAFEKVQEMRYGENPHQSAAFYRDLKAVD
GALANYVQLQGKELSYNNIADADAAWECVKSFAVTTPACVIIKHANPCGVAVGANALEAYDKAFKTDSTSAFGGIIAFNV
ELDETAAQAVAKQFVEVLIAPSFSAAARAVFASKQNVRLLEIPLGKGINQYDLKRVGGGLLVQSPDAKNVQPTELRVVTR
RHPTPKEMDDLMFAWRVAKFVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLD
VVVDAGATCVIQPGGSMRDDEVIAAADDRGIAMVLTGTRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=478, Percent_Identity=38.2845188284519, Blast_Score=279, Evalue=5e-75,
Organism=Escherichia coli, GI1790439, Length=527, Percent_Identity=61.6698292220114, Blast_Score=643, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=609, Percent_Identity=32.8407224958949, Blast_Score=284, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI71985574, Length=315, Percent_Identity=27.3015873015873, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985556, Length=130, Percent_Identity=33.8461538461538, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323768, Length=484, Percent_Identity=36.3636363636364, Blast_Score=264, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6323056, Length=487, Percent_Identity=35.9342915811088, Blast_Score=258, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24649832, Length=485, Percent_Identity=36.701030927835, Blast_Score=259, Evalue=2e-69,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55980; Mature: 55980

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKQALLSVSDKTGIVEFARELNALGVTLLSTGGTAKLLADSGLPVTEVADYTGFPEMLD
CCHHHHHHCCCCCHHHHHHHHHHHCEEEEEECCCCEEEEECCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMAALAEHDIPTIDLLVVNLYPFQQTVAKDDCTLPDAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEEEEEEEECHHHHHCCCCCCCCHHH
ENIDIGGPTMLRSAAKNHRDVTVIVDPVDYAVVLDEMRANGNKVGYDTNFRLATKVFAHT
HCCCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
AQYDGAITNYLTSLGADKSHQARSAYPQTLNLAFEKVQEMRYGENPHQSAAFYRDLKAVD
HHCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GALANYVQLQGKELSYNNIADADAAWECVKSFAVTTPACVIIKHANPCGVAVGANALEAY
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCHHHHHH
DKAFKTDSTSAFGGIIAFNVELDETAAQAVAKQFVEVLIAPSFSAAARAVFASKQNVRLL
HHHHCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEE
EIPLGKGINQYDLKRVGGGLLVQSPDAKNVQPTELRVVTRRHPTPKEMDDLMFAWRVAKF
EEECCCCCCHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
VKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLD
HHCCEEEEECCCEEEEECCCHHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCCE
VVVDAGATCVIQPGGSMRDDEVIAAADDRGIAMVLTGTRHFRH
EEEECCCEEEECCCCCCCCCCEEEEECCCCEEEEEECCHHCCC
>Mature Secondary Structure
MIKQALLSVSDKTGIVEFARELNALGVTLLSTGGTAKLLADSGLPVTEVADYTGFPEMLD
CCHHHHHHCCCCCHHHHHHHHHHHCEEEEEECCCCEEEEECCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMAALAEHDIPTIDLLVVNLYPFQQTVAKDDCTLPDAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEEEEEEEECHHHHHCCCCCCCCHHH
ENIDIGGPTMLRSAAKNHRDVTVIVDPVDYAVVLDEMRANGNKVGYDTNFRLATKVFAHT
HCCCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
AQYDGAITNYLTSLGADKSHQARSAYPQTLNLAFEKVQEMRYGENPHQSAAFYRDLKAVD
HHCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GALANYVQLQGKELSYNNIADADAAWECVKSFAVTTPACVIIKHANPCGVAVGANALEAY
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCHHHHHH
DKAFKTDSTSAFGGIIAFNVELDETAAQAVAKQFVEVLIAPSFSAAARAVFASKQNVRLL
HHHHCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEE
EIPLGKGINQYDLKRVGGGLLVQSPDAKNVQPTELRVVTRRHPTPKEMDDLMFAWRVAKF
EEECCCCCCHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
VKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLD
HHCCEEEEECCCEEEEECCCHHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCCE
VVVDAGATCVIQPGGSMRDDEVIAAADDRGIAMVLTGTRHFRH
EEEECCCEEEECCCCCCCCCCEEEEECCCCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA