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The map label for this gene is ruvC

Identifier: 73540192

GI number: 73540192

Start: 528940

End: 529485

Strand: Reverse

Name: ruvC

Synonym: Reut_A0486

Alternate gene names: 73540192

Gene position: 529485-528940 (Counterclockwise)

Preceding gene: 73540193

Following gene: 73540191

Centisome position: 13.91

GC content: 63.74

Gene sequence:

>546_bases
ATGCGTATTCTCGGCATCGACCCCGGCCTCCGCACCACCGGCTTCGGCGTCCTGGAAAAGCACGGCAACAAGCTTTCCTA
CATCGCCTCGGGCACCATCAAAAGCAATGGTGAAACCGACCTGCCCGCGCGCCTGAAGACACTGTTCGATGGCATCAGCG
AGGTAGCCCGCACGTACACACCGGATTGCGCAGCGATTGAAAAGGTCTTCGTCAACGTCAACCCGCAGTCCACGCTGCTA
CTAGGTCAAGCACGCGGCGCGGCAATCTGCGGCCTGGTCGGCCAAGGCCTGCCGGTGTTCGAATACACCGCCCTGCAACT
GAAGGTAGCCGTAGTCGGCTATGGCCGCGCCAACAAGGAACAAGTGCAGGAAATGGTCATGCGTCTGCTAAGCCTGCCGG
GACGGCCCAGCACTGACGCCGCCGATGCATTGGGCGTCGCGATCTGCCACGCCAATGGCGGCGACACGCTCGGCACGCTG
TCGGGCCTCGCCCCTGAACTGGTCCGCAAGGGCATGCGCGTCCGCCGAGGGCGCCTGATCGGCTGA

Upstream 100 bases:

>100_bases
TCGGACAAAAAGTGACTCGCCGGCTACGCCGGCGAAACAGCGGCGCAAGTCAAGAACAAAGCCCGATCAAAGCCTGACAA
AGCAACCCCAAAAAACATAA

Downstream 100 bases:

>100_bases
TCGGCCAACATCGGTCTCCACAAGAGACAGCGCCCGGTCTCTTACAATCGAGGGCATTTGCCGCGCACGGCGGCCACGGA
TCTTATTCCATGCCCAGCTT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MRILGIDPGLRTTGFGVLEKHGNKLSYIASGTIKSNGETDLPARLKTLFDGISEVARTYTPDCAAIEKVFVNVNPQSTLL
LGQARGAAICGLVGQGLPVFEYTALQLKVAVVGYGRANKEQVQEMVMRLLSLPGRPSTDAADALGVAICHANGGDTLGTL
SGLAPELVRKGMRVRRGRLIG

Sequences:

>Translated_181_residues
MRILGIDPGLRTTGFGVLEKHGNKLSYIASGTIKSNGETDLPARLKTLFDGISEVARTYTPDCAAIEKVFVNVNPQSTLL
LGQARGAAICGLVGQGLPVFEYTALQLKVAVVGYGRANKEQVQEMVMRLLSLPGRPSTDAADALGVAICHANGGDTLGTL
SGLAPELVRKGMRVRRGRLIG
>Mature_181_residues
MRILGIDPGLRTTGFGVLEKHGNKLSYIASGTIKSNGETDLPARLKTLFDGISEVARTYTPDCAAIEKVFVNVNPQSTLL
LGQARGAAICGLVGQGLPVFEYTALQLKVAVVGYGRANKEQVQEMVMRLLSLPGRPSTDAADALGVAICHANGGDTLGTL
SGLAPELVRKGMRVRRGRLIG

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=177, Percent_Identity=49.1525423728814, Blast_Score=142, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_CUPPJ (Q475R5)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294712.1
- ProteinModelPortal:   Q475R5
- SMR:   Q475R5
- GeneID:   3611790
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0486
- NMPDR:   fig|264198.3.peg.998
- HOGENOM:   HBG437552
- OMA:   DCAAIEK
- ProtClustDB:   PRK00039
- BioCyc:   REUT264198:REUT_A0486-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 19088; Mature: 19088

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILGIDPGLRTTGFGVLEKHGNKLSYIASGTIKSNGETDLPARLKTLFDGISEVARTYT
CEEEECCCCCCCCCCHHHHHCCCEEEEEEECEECCCCCCCHHHHHHHHHHHHHHHHHHCC
PDCAAIEKVFVNVNPQSTLLLGQARGAAICGLVGQGLPVFEYTALQLKVAVVGYGRANKE
CCHHHHHHHHEECCCCCEEEEECCCCCEEEHHHCCCCCCEEEHEEEEEEEEEECCCCCHH
QVQEMVMRLLSLPGRPSTDAADALGVAICHANGGDTLGTLSGLAPELVRKGMRVRRGRLI
HHHHHHHHHHCCCCCCCCCHHHHHEEEEEECCCCCCHHHHHCCCHHHHHHHHHHHCCCCC
G
C
>Mature Secondary Structure
MRILGIDPGLRTTGFGVLEKHGNKLSYIASGTIKSNGETDLPARLKTLFDGISEVARTYT
CEEEECCCCCCCCCCHHHHHCCCEEEEEEECEECCCCCCCHHHHHHHHHHHHHHHHHHCC
PDCAAIEKVFVNVNPQSTLLLGQARGAAICGLVGQGLPVFEYTALQLKVAVVGYGRANKE
CCHHHHHHHHEECCCCCEEEEECCCCCEEEHHHCCCCCCEEEHEEEEEEEEEECCCCCHH
QVQEMVMRLLSLPGRPSTDAADALGVAICHANGGDTLGTLSGLAPELVRKGMRVRRGRLI
HHHHHHHHHHCCCCCCCCCHHHHHEEEEEECCCCCCHHHHHCCCHHHHHHHHHHHCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA