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The map label for this gene is tyrS

Identifier: 73540182

GI number: 73540182

Start: 520417

End: 521658

Strand: Direct

Name: tyrS

Synonym: Reut_A0476

Alternate gene names: 73540182

Gene position: 520417-521658 (Clockwise)

Preceding gene: 73540179

Following gene: 73540183

Centisome position: 13.67

GC content: 65.06

Gene sequence:

>1242_bases
ATGACTGAAGCTTCCTCCGGCCCCGCGGCCAAATATCCCCTCACGCCGTCGGTGATGCACGCGCTGGAGGTGTCCAAGCG
CGGCTGCGACGAACTGCTGATCGAAGCCGAATGGCTGCAGAAGCTGGCCCGCAGCGAGGCGACCGGCGTTCCGCTGCGCA
TCAAGCTCGGCCTCGACCCCACCGCGCCCGACATCCACATCGGCCATACGGTGGTTCTGAACAAGCTGCGTCAGCTGCAA
GACCTGGGCCATCAGGTGATTTTCCTGATCGGCGACTTCACCTCGACCATCGGCGACCCGTCCGGCCGCAACAGCACCCG
GCCGCCGCTGACGCGCGAGCAGATCGAGGCCAACGCCCAGACTTACTACCGCCAGGCCAGCCTGGTGCTCGATCCGGCCC
GCACCGAAATCCGTTACAACAGCGAGTGGTGTGATCCGCTCGGCGCACGCGGCATGATCCAGCTCGCCGCCAAGTACACC
GTGGCCCGGATGATGGAGCGTGACGACTTCACCAAACGGTTCCGGTCTGGGATTCCCATTTCGGTGCATGAGTTCCTTTA
TCCGCTCATGCAGGGCTACGATTCGGTCGCGCTGAAGTCCGATCTGGAGCTGGGCGGCACCGACCAGAAGTTCAACCTGC
TGGTCGGGCGCGAGCTGCAGAAGGAATACGGCCAGGAGCCGCAGTGCATCCTGACCATGCCTCTGCTGGTCGGCCTGGAC
GGTGTCGAGAAGATGTCGAAGTCCAAGGGCAACTACGTTGGCGTGACCGAGGCGCCCAACGAGATGTTCGGCAAGCTGAT
GAGCATTTCGGACGACCTGATGTGGCAGTACTTCACGCTGCTGTCGTTCCGCCCGCTCGCCGAGATCGACCTGATGAAGC
AGGAAATCGCGGCCGGCCGCAATCCGCGCGACTGCAAGGTGCTGCTGGCGCAGGAGATCGTGGCCCGCTTCCACAGCCAG
GCCGATGCCGAAAAGGCGCTCGAGGACTTCAACCACCGCGCGCGCGGCGGCGTGCCTGACGACATCCCGGCAGTCAGCCT
GTCGGGCGCCCCGCTGGGCATCGCGCAACTGCTCAAGCAGGCCAACCTGGTGCCGTCCACGTCCGAGGCCAACCGCAATA
TCGAGCAGGGCGGCGTGAAGATCGATGGCGCGACCGTCAGCGACAAGGCCGTCAAGGTGGCCGCGGGCACCTACGTGGTG
CAGGTCGGCAAGCGCCGCTTTGCCCGCGTGACGCTGGCCTGA

Upstream 100 bases:

>100_bases
CGGAGCCACGTTGTGCCGCAAAGCGCGGATTATACCAAAAACCGGCCCGGGGCCGAGTGGCGCATCCGCCAAATTTTCAT
GTATTTCAAAGACTTACGTC

Downstream 100 bases:

>100_bases
GGCGGGCGATGATCGCACTGATCCAGCGCGTCTCGCAGGCCCGCGTGACGGTCGAGGGCCGCACCACCGGAGAGATTGGC
GCCGGGCTGCTGGCGCTGGT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MTEASSGPAAKYPLTPSVMHALEVSKRGCDELLIEAEWLQKLARSEATGVPLRIKLGLDPTAPDIHIGHTVVLNKLRQLQ
DLGHQVIFLIGDFTSTIGDPSGRNSTRPPLTREQIEANAQTYYRQASLVLDPARTEIRYNSEWCDPLGARGMIQLAAKYT
VARMMERDDFTKRFRSGIPISVHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQEPQCILTMPLLVGLD
GVEKMSKSKGNYVGVTEAPNEMFGKLMSISDDLMWQYFTLLSFRPLAEIDLMKQEIAAGRNPRDCKVLLAQEIVARFHSQ
ADAEKALEDFNHRARGGVPDDIPAVSLSGAPLGIAQLLKQANLVPSTSEANRNIEQGGVKIDGATVSDKAVKVAAGTYVV
QVGKRRFARVTLA

Sequences:

>Translated_413_residues
MTEASSGPAAKYPLTPSVMHALEVSKRGCDELLIEAEWLQKLARSEATGVPLRIKLGLDPTAPDIHIGHTVVLNKLRQLQ
DLGHQVIFLIGDFTSTIGDPSGRNSTRPPLTREQIEANAQTYYRQASLVLDPARTEIRYNSEWCDPLGARGMIQLAAKYT
VARMMERDDFTKRFRSGIPISVHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQEPQCILTMPLLVGLD
GVEKMSKSKGNYVGVTEAPNEMFGKLMSISDDLMWQYFTLLSFRPLAEIDLMKQEIAAGRNPRDCKVLLAQEIVARFHSQ
ADAEKALEDFNHRARGGVPDDIPAVSLSGAPLGIAQLLKQANLVPSTSEANRNIEQGGVKIDGATVSDKAVKVAAGTYVV
QVGKRRFARVTLA
>Mature_412_residues
TEASSGPAAKYPLTPSVMHALEVSKRGCDELLIEAEWLQKLARSEATGVPLRIKLGLDPTAPDIHIGHTVVLNKLRQLQD
LGHQVIFLIGDFTSTIGDPSGRNSTRPPLTREQIEANAQTYYRQASLVLDPARTEIRYNSEWCDPLGARGMIQLAAKYTV
ARMMERDDFTKRFRSGIPISVHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQEPQCILTMPLLVGLDG
VEKMSKSKGNYVGVTEAPNEMFGKLMSISDDLMWQYFTLLSFRPLAEIDLMKQEIAAGRNPRDCKVLLAQEIVARFHSQA
DAEKALEDFNHRARGGVPDDIPAVSLSGAPLGIAQLLKQANLVPSTSEANRNIEQGGVKIDGATVSDKAVKVAAGTYVVQ
VGKRRFARVTLA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=407, Percent_Identity=27.5184275184275, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1787925, Length=404, Percent_Identity=27.2277227722772, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25145802, Length=261, Percent_Identity=28.3524904214559, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6325160, Length=425, Percent_Identity=24.2352941176471, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20130383, Length=401, Percent_Identity=26.4339152119701, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_CUPPJ (Q475S5)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294702.1
- ProteinModelPortal:   Q475S5
- SMR:   Q475S5
- GeneID:   3611385
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0476
- NMPDR:   fig|264198.3.peg.988
- HOGENOM:   HBG288125
- OMA:   YVVQVGK
- ProtClustDB:   PRK05912
- BioCyc:   REUT264198:REUT_A0476-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 45466; Mature: 45335

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 247-247

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEASSGPAAKYPLTPSVMHALEVSKRGCDELLIEAEWLQKLARSEATGVPLRIKLGLDP
CCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
TAPDIHIGHTVVLNKLRQLQDLGHQVIFLIGDFTSTIGDPSGRNSTRPPLTREQIEANAQ
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHCCCCCCCCCCCCCCCHHHHHHHHH
TYYRQASLVLDPARTEIRYNSEWCDPLGARGMIQLAAKYTVARMMERDDFTKRFRSGIPI
HHHHHHHEEECCCHHCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
SVHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQEPQCILTMPLLVGLD
EHHHHHHHHHHCCCCEEHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCC
GVEKMSKSKGNYVGVTEAPNEMFGKLMSISDDLMWQYFTLLSFRPLAEIDLMKQEIAAGR
HHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
NPRDCKVLLAQEIVARFHSQADAEKALEDFNHRARGGVPDDIPAVSLSGAPLGIAQLLKQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHH
ANLVPSTSEANRNIEQGGVKIDGATVSDKAVKVAAGTYVVQVGKRRFARVTLA
CCCCCCCCHHCCCHHHCCEEECCCEECCCCEEEECCHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
TEASSGPAAKYPLTPSVMHALEVSKRGCDELLIEAEWLQKLARSEATGVPLRIKLGLDP
CCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
TAPDIHIGHTVVLNKLRQLQDLGHQVIFLIGDFTSTIGDPSGRNSTRPPLTREQIEANAQ
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHCCCCCCCCCCCCCCCHHHHHHHHH
TYYRQASLVLDPARTEIRYNSEWCDPLGARGMIQLAAKYTVARMMERDDFTKRFRSGIPI
HHHHHHHEEECCCHHCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
SVHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQEPQCILTMPLLVGLD
EHHHHHHHHHHCCCCEEHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCC
GVEKMSKSKGNYVGVTEAPNEMFGKLMSISDDLMWQYFTLLSFRPLAEIDLMKQEIAAGR
HHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
NPRDCKVLLAQEIVARFHSQADAEKALEDFNHRARGGVPDDIPAVSLSGAPLGIAQLLKQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHH
ANLVPSTSEANRNIEQGGVKIDGATVSDKAVKVAAGTYVVQVGKRRFARVTLA
CCCCCCCCHHCCCHHHCCEEECCCEECCCCEEEECCHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA