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The map label for this gene is dmlA [H]

Identifier: 73540168

GI number: 73540168

Start: 507871

End: 508944

Strand: Direct

Name: dmlA [H]

Synonym: Reut_A0462

Alternate gene names: 73540168

Gene position: 507871-508944 (Clockwise)

Preceding gene: 73540166

Following gene: 73540169

Centisome position: 13.34

GC content: 64.62

Gene sequence:

>1074_bases
ATGAGCCAATACAGGATTGCCGTGATTCCCGGAGACGGAATCGGCACGGAAGTGATGCCCGAGGGCATTCGTGTCATGGA
TGCCGCCGCGCGCAAGTTCGGCATCGACTTCCAGTGGGACCACTTCGATTTTTCCAGCTGCGACTACTACGCGCGCCACG
GCAAGATGTTGCCGGACGACTGGTTCGACACGCTCGTGAAGTACGACGCGATCTATTTTGGCGCGGTGGGCTGGCCCGAT
GCCGTGCCCGATCACGTGTCGCTGTGGGGCTCGCTGCTGCAGTTCCGCCGCTCGTTCGACCAGTACGTAAACCTGCGTCC
GGTTCGGCTGATGCCCGGCATCAAGAGCCCGCTGGCCGACCGCAAGCCCGGCGACATCGACTTCTATGTCGTGCGCGAGA
ACACCGAGGGCGAGTACTCGAGCATCGGCGGCCGCATGTTCCCGAACACCGAGCGCGAGATCGTGATGCAGGAAACGGTG
ATGAGCCGGGTCGGCGTTGACCGCATCCTGAAATTCGCCTTCGAGTTGGCGAAGAAGCGTCCGAAACAGCACCTGACGTC
GGCAACCAAGTCCAACGGCATCTCGATCACCATGCCGTACTGGGACGAGCGCGTGGAGGCGATGGCCGCGAACTATCCCG
GCATCAAGGTCGACAAGTACCACATCGACATCCTGACCGCGCATTTCGTCCAGCATCCCGACTGGTTCGACGTGGTGGTG
GCGAGCAACCTGTTTGGCGACATCCTGTCCGACCTGGGGCCGGCGTGTACCGGCACCATCGGCATCGCGCCGTCGGGCAA
TATCAACCCGGACCGCACGTATCCGAGCCTGTTCGAGCCGGTGCATGGCTCGGCGCCCGACATCGCGGGTCGCGGTGTGG
CCAACCCGATCGGCCAGATCTGGTGCGGCGCCATGATGCTGGAGCACCTCGGGCACGACGCGGCGGGTGCCGCCGTGCTG
GGCGCCATCGAGAAGGTGTTGTCCGAGGGCCCCGACCATGCGCCGCTGACGCGCGATATCGGCGGCAAGGCGGGCACCGA
GGACCTGGGCCGCGCAATCGCGGAGGCGCTGTGA

Upstream 100 bases:

>100_bases
TTTGCAATAGAATGCAGACATTATCCCGCGCCCCCGGGCCGCTGCCAAGCGCACGCGTCCGGGCGCCTCAGGCCATCCCG
CACCACCCTTCCCGACAGCT

Downstream 100 bases:

>100_bases
GCGCGCCGGGGAGTTTCACGGGCGATCCGCGCGCGCTGCTGCTCGAGACGTTCCAGGCGGCGGTGGCGGCGGCGGATCCG
CTGAAGATCGTGGCCGAGTA

Product: tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSQYRIAVIPGDGIGTEVMPEGIRVMDAAARKFGIDFQWDHFDFSSCDYYARHGKMLPDDWFDTLVKYDAIYFGAVGWPD
AVPDHVSLWGSLLQFRRSFDQYVNLRPVRLMPGIKSPLADRKPGDIDFYVVRENTEGEYSSIGGRMFPNTEREIVMQETV
MSRVGVDRILKFAFELAKKRPKQHLTSATKSNGISITMPYWDERVEAMAANYPGIKVDKYHIDILTAHFVQHPDWFDVVV
ASNLFGDILSDLGPACTGTIGIAPSGNINPDRTYPSLFEPVHGSAPDIAGRGVANPIGQIWCGAMMLEHLGHDAAGAAVL
GAIEKVLSEGPDHAPLTRDIGGKAGTEDLGRAIAEAL

Sequences:

>Translated_357_residues
MSQYRIAVIPGDGIGTEVMPEGIRVMDAAARKFGIDFQWDHFDFSSCDYYARHGKMLPDDWFDTLVKYDAIYFGAVGWPD
AVPDHVSLWGSLLQFRRSFDQYVNLRPVRLMPGIKSPLADRKPGDIDFYVVRENTEGEYSSIGGRMFPNTEREIVMQETV
MSRVGVDRILKFAFELAKKRPKQHLTSATKSNGISITMPYWDERVEAMAANYPGIKVDKYHIDILTAHFVQHPDWFDVVV
ASNLFGDILSDLGPACTGTIGIAPSGNINPDRTYPSLFEPVHGSAPDIAGRGVANPIGQIWCGAMMLEHLGHDAAGAAVL
GAIEKVLSEGPDHAPLTRDIGGKAGTEDLGRAIAEAL
>Mature_356_residues
SQYRIAVIPGDGIGTEVMPEGIRVMDAAARKFGIDFQWDHFDFSSCDYYARHGKMLPDDWFDTLVKYDAIYFGAVGWPDA
VPDHVSLWGSLLQFRRSFDQYVNLRPVRLMPGIKSPLADRKPGDIDFYVVRENTEGEYSSIGGRMFPNTEREIVMQETVM
SRVGVDRILKFAFELAKKRPKQHLTSATKSNGISITMPYWDERVEAMAANYPGIKVDKYHIDILTAHFVQHPDWFDVVVA
SNLFGDILSDLGPACTGTIGIAPSGNINPDRTYPSLFEPVHGSAPDIAGRGVANPIGQIWCGAMMLEHLGHDAAGAAVLG
AIEKVLSEGPDHAPLTRDIGGKAGTEDLGRAIAEAL

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=350, Percent_Identity=32.2857142857143, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI28178816, Length=356, Percent_Identity=27.5280898876405, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI28178821, Length=348, Percent_Identity=26.7241379310345, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI4758582, Length=346, Percent_Identity=25.7225433526012, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI28178838, Length=338, Percent_Identity=26.3313609467456, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI28178819, Length=210, Percent_Identity=28.5714285714286, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1788101, Length=362, Percent_Identity=65.4696132596685, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI87081683, Length=359, Percent_Identity=36.7688022284123, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1787381, Length=367, Percent_Identity=26.7029972752044, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17550882, Length=354, Percent_Identity=30.225988700565, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI71986051, Length=339, Percent_Identity=32.4483775811209, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25144293, Length=352, Percent_Identity=25.2840909090909, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17505779, Length=357, Percent_Identity=25.7703081232493, Blast_Score=104, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6322097, Length=362, Percent_Identity=35.3591160220994, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6324709, Length=358, Percent_Identity=29.0502793296089, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319830, Length=362, Percent_Identity=32.0441988950276, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6324291, Length=359, Percent_Identity=28.6908077994429, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24643268, Length=354, Percent_Identity=31.0734463276836, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24643270, Length=354, Percent_Identity=31.0734463276836, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24661184, Length=358, Percent_Identity=28.4916201117318, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI281362242, Length=356, Percent_Identity=29.2134831460674, Blast_Score=141, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24648872, Length=356, Percent_Identity=29.2134831460674, Blast_Score=141, Evalue=8e-34,
Organism=Drosophila melanogaster, GI161078635, Length=360, Percent_Identity=25.8333333333333, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI161078637, Length=360, Percent_Identity=25.8333333333333, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI161078633, Length=360, Percent_Identity=25.5555555555556, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24650122, Length=360, Percent_Identity=25.5555555555556, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20130355, Length=355, Percent_Identity=25.9154929577465, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161078639, Length=266, Percent_Identity=27.0676691729323, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 39314; Mature: 39183

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQYRIAVIPGDGIGTEVMPEGIRVMDAAARKFGIDFQWDHFDFSSCDYYARHGKMLPDD
CCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHCCCCCCHH
WFDTLVKYDAIYFGAVGWPDAVPDHVSLWGSLLQFRRSFDQYVNLRPVRLMPGIKSPLAD
HHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
RKPGDIDFYVVRENTEGEYSSIGGRMFPNTEREIVMQETVMSRVGVDRILKFAFELAKKR
CCCCCEEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHC
PKQHLTSATKSNGISITMPYWDERVEAMAANYPGIKVDKYHIDILTAHFVQHPDWFDVVV
HHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHCCCCHHHHHH
ASNLFGDILSDLGPACTGTIGIAPSGNINPDRTYPSLFEPVHGSAPDIAGRGVANPIGQI
HHHHHHHHHHHCCCHHCCCEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHH
WCGAMMLEHLGHDAAGAAVLGAIEKVLSEGPDHAPLTRDIGGKAGTEDLGRAIAEAL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure 
SQYRIAVIPGDGIGTEVMPEGIRVMDAAARKFGIDFQWDHFDFSSCDYYARHGKMLPDD
CCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHCCCCCCHH
WFDTLVKYDAIYFGAVGWPDAVPDHVSLWGSLLQFRRSFDQYVNLRPVRLMPGIKSPLAD
HHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
RKPGDIDFYVVRENTEGEYSSIGGRMFPNTEREIVMQETVMSRVGVDRILKFAFELAKKR
CCCCCEEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHC
PKQHLTSATKSNGISITMPYWDERVEAMAANYPGIKVDKYHIDILTAHFVQHPDWFDVVV
HHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHCCCCHHHHHH
ASNLFGDILSDLGPACTGTIGIAPSGNINPDRTYPSLFEPVHGSAPDIAGRGVANPIGQI
HHHHHHHHHHHCCCHHCCCEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHH
WCGAMMLEHLGHDAAGAAVLGAIEKVLSEGPDHAPLTRDIGGKAGTEDLGRAIAEAL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]