Click here to switch to the map view.

The map label for this gene is rhtB [C]

Identifier: 73540070

GI number: 73540070

Start: 393326

End: 393937

Strand: Direct

Name: rhtB [C]

Synonym: Reut_A0364

Alternate gene names: 73540070

Gene position: 393326-393937 (Clockwise)

Preceding gene: 73540069

Following gene: 73540071

Centisome position: 10.33

GC content: 68.79

Gene sequence:

>612_bases
ATGCCCGACCTGCTGCTGTTCCTTGTGACCTCGATCGCCATCACCCTGGCCCCCGGCCCGGACAACCTGCAAGTGCTGGC
GCGCGGCATGGCACAGGGGCGCCGCGCAGGCGTGGTGGCAGCACTCGGGTTTGCCGTCGGCTGCCTGTTCCATACGATGA
TTGCCGCGGTCGGGCTCGCGGCGGTGCTGCGCTCGTCGCCGCTGGCGTTCCAGCTGATCAAGTACGCCGGCGCGGCTTAC
CTGATCTGGATCGGCATCCAGGCGCTGCGTGCGAAGGGCGGACTCGCACAGGGCGCCGACGTGGCCGCGGTGCCGCTTGG
CAAGGTGTTCCGGCAGAGCGTGCTCGGCAACATGATGAACCCCAAGGTCACGCTGTTCTTCCTCGTGTTCCTGCCGCAGT
TCGTGCATGCCGATGCCACGCACCCGGCCTTGCAGTTCGTGCTGCTGGGCCTGGTGTTCATGCTGCAGACGGCCGTGGTG
TTCTCGCTGTTCGGCCTGTGCGCGGGCTGGCTCGGCGCATGGCTGCGCAAGCGGCCGACCACGGGGCGCTGGCTTGACCG
CGCGGCCGGCGCGATCTTCGTCGGCCTCGGCATCAAGGTGGCGCTTCCCTGA

Upstream 100 bases:

>100_bases
CTGCTGCAGAATGCGGGTTTGCCTCTGTTCACGGTGTGCCGGTTCGATGGCCACTAATATGCCCGTGCGCGCTGCCTGAT
CCTTTCCGACGGAGATTTTC

Downstream 100 bases:

>100_bases
GCGCTGCGCGGGCGTGCTGCCCGCGCCCCTTGGCGTTGCCCCGGTTACATGCGGGGCGCATAATGGCCCATTGACAACAA
CATGATGACCGGGCGGAGCC

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MPDLLLFLVTSIAITLAPGPDNLQVLARGMAQGRRAGVVAALGFAVGCLFHTMIAAVGLAAVLRSSPLAFQLIKYAGAAY
LIWIGIQALRAKGGLAQGADVAAVPLGKVFRQSVLGNMMNPKVTLFFLVFLPQFVHADATHPALQFVLLGLVFMLQTAVV
FSLFGLCAGWLGAWLRKRPTTGRWLDRAAGAIFVGLGIKVALP

Sequences:

>Translated_203_residues
MPDLLLFLVTSIAITLAPGPDNLQVLARGMAQGRRAGVVAALGFAVGCLFHTMIAAVGLAAVLRSSPLAFQLIKYAGAAY
LIWIGIQALRAKGGLAQGADVAAVPLGKVFRQSVLGNMMNPKVTLFFLVFLPQFVHADATHPALQFVLLGLVFMLQTAVV
FSLFGLCAGWLGAWLRKRPTTGRWLDRAAGAIFVGLGIKVALP
>Mature_202_residues
PDLLLFLVTSIAITLAPGPDNLQVLARGMAQGRRAGVVAALGFAVGCLFHTMIAAVGLAAVLRSSPLAFQLIKYAGAAYL
IWIGIQALRAKGGLAQGADVAAVPLGKVFRQSVLGNMMNPKVTLFFLVFLPQFVHADATHPALQFVLLGLVFMLQTAVVF
SLFGLCAGWLGAWLRKRPTTGRWLDRAAGAIFVGLGIKVALP

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=192, Percent_Identity=30.2083333333333, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788099, Length=196, Percent_Identity=27.5510204081633, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI48994978, Length=209, Percent_Identity=29.6650717703349, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1786522, Length=124, Percent_Identity=29.0322580645161, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21437; Mature: 21306

Theoretical pI: Translated: 11.54; Mature: 11.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLLLFLVTSIAITLAPGPDNLQVLARGMAQGRRAGVVAALGFAVGCLFHTMIAAVGLA
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLRSSPLAFQLIKYAGAAYLIWIGIQALRAKGGLAQGADVAAVPLGKVFRQSVLGNMMN
HHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PKVTLFFLVFLPQFVHADATHPALQFVLLGLVFMLQTAVVFSLFGLCAGWLGAWLRKRPT
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TGRWLDRAAGAIFVGLGIKVALP
CCHHHHHHHHHEEEECCEEEECC
>Mature Secondary Structure 
PDLLLFLVTSIAITLAPGPDNLQVLARGMAQGRRAGVVAALGFAVGCLFHTMIAAVGLA
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLRSSPLAFQLIKYAGAAYLIWIGIQALRAKGGLAQGADVAAVPLGKVFRQSVLGNMMN
HHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PKVTLFFLVFLPQFVHADATHPALQFVLLGLVFMLQTAVVFSLFGLCAGWLGAWLRKRPT
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TGRWLDRAAGAIFVGLGIKVALP
CCHHHHHHHHHEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8169201; 10984043 [H]