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The map label for this gene is ptsN [H]

Identifier: 73540059

GI number: 73540059

Start: 383384

End: 383839

Strand: Reverse

Name: ptsN [H]

Synonym: Reut_A0353

Alternate gene names: 73540059

Gene position: 383839-383384 (Counterclockwise)

Preceding gene: 73540060

Following gene: 73540058

Centisome position: 10.08

GC content: 62.06

Gene sequence:

>456_bases
ATGAATCGTTTGGCCAAACTGCTGCCACCCGGCAACATCAACCTCGACGTCAGCGTCACCAGCAAGAAGCGTGTCTTCGA
GCAAGCCGGGCTCCTCTTCGAGAACAACCATGGCGTGGCGCGGGCGACCGTGACCGACAACCTGTTTGCGCGCGAGTCGC
TCGGTTCGACCGGCCTTGGCGCAGGCGTGGCCATTCCGCATGGGCGCATCAAGGGACTGAAGCAGCCGTTGGCCGCGTTC
ATGCGGTTGGCCGAGCCGATCCCTTTCGAATCACCCGACGGCAAACCCGTTTCGCTGCTGATCTTCCTGCTGGTGCCGGA
ACAGGCCACGCAGCAGCATCTCGAGATCCTCTCGGAAATCGCGCAGTTGCTGTCCGATCGCGACATGCGCGAAGGCCTCG
CAACGCTGGCCACACCCGAAGAAGTGCATCAGCTACTGACACAGTGGCATCCCTGA

Upstream 100 bases:

>100_bases
CCAAACGCTTGGCAAACCATGGTATCGCCATAAAGCGGCCCGCAGCCTTGCACGGCCGAGCAGTCCCATCTGCCTGCTAA
CTGAAATCCTCGCATCGCCC

Downstream 100 bases:

>100_bases
CCCTTCCCCTGGGCTGGCAACAGACCGGACGACCCTAAAGCCCGGCACTGCCCCCGCCCCGAAAGTCATACCGCATGGAA
CTCACCGGCGTCACCTCGCA

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MNRLAKLLPPGNINLDVSVTSKKRVFEQAGLLFENNHGVARATVTDNLFARESLGSTGLGAGVAIPHGRIKGLKQPLAAF
MRLAEPIPFESPDGKPVSLLIFLLVPEQATQQHLEILSEIAQLLSDRDMREGLATLATPEEVHQLLTQWHP

Sequences:

>Translated_151_residues
MNRLAKLLPPGNINLDVSVTSKKRVFEQAGLLFENNHGVARATVTDNLFARESLGSTGLGAGVAIPHGRIKGLKQPLAAF
MRLAEPIPFESPDGKPVSLLIFLLVPEQATQQHLEILSEIAQLLSDRDMREGLATLATPEEVHQLLTQWHP
>Mature_151_residues
MNRLAKLLPPGNINLDVSVTSKKRVFEQAGLLFENNHGVARATVTDNLFARESLGSTGLGAGVAIPHGRIKGLKQPLAAF
MRLAEPIPFESPDGKPVSLLIFLLVPEQATQQHLEILSEIAQLLSDRDMREGLATLATPEEVHQLLTQWHP

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=132, Percent_Identity=36.3636363636364, Blast_Score=86, Evalue=9e-19,
Organism=Escherichia coli, GI1786951, Length=99, Percent_Identity=34.3434343434343, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI2367327, Length=116, Percent_Identity=30.1724137931034, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1788726, Length=145, Percent_Identity=29.6551724137931, Blast_Score=60, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16487; Mature: 16487

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLAKLLPPGNINLDVSVTSKKRVFEQAGLLFENNHGVARATVTDNLFARESLGSTGLG
CCCCHHHCCCCCCEEEEEECHHHHHHHHHCCEEECCCCEEEEEHHHHHHHHHHCCCCCCC
AGVAIPHGRIKGLKQPLAAFMRLAEPIPFESPDGKPVSLLIFLLVPEQATQQHLEILSEI
CCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEEEEEECCCHHHHHHHHHHHHH
AQLLSDRDMREGLATLATPEEVHQLLTQWHP
HHHHCCCHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNRLAKLLPPGNINLDVSVTSKKRVFEQAGLLFENNHGVARATVTDNLFARESLGSTGLG
CCCCHHHCCCCCCEEEEEECHHHHHHHHHCCEEECCCCEEEEEHHHHHHHHHHCCCCCCC
AGVAIPHGRIKGLKQPLAAFMRLAEPIPFESPDGKPVSLLIFLLVPEQATQQHLEILSEI
CCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEEEEEECCCHHHHHHHHHHHHH
AQLLSDRDMREGLATLATPEEVHQLLTQWHP
HHHHCCCHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]