Click here to switch to the map view.

The map label for this gene is ipk

Identifier: 73540049

GI number: 73540049

Start: 372099

End: 372989

Strand: Reverse

Name: ipk

Synonym: Reut_A0343

Alternate gene names: 73540049

Gene position: 372989-372099 (Counterclockwise)

Preceding gene: 73540050

Following gene: 73540048

Centisome position: 9.8

GC content: 64.98

Gene sequence:

>891_bases
TTGACCGCAGCCTTGACCGCGGCACCCACCCTGCCGCCGCCGGAACTGCGCGACTGCCCCGCGCCAGCCAAGCTCAACCT
GTTCCTGCACGTCACGGGCCGGCGCCCGGACGGCTACCACATGCTGCAGACGGTATTCCAGCTCGTGGACTGGTGCGACA
CGCTGCATTTCCGCCGCCGCGATGACGGCGTCGTGACGCGCACCACCGACATCCCGGGCGTACCGGCGGAGACCGACCTG
ATCGTGCGTGCCGCGCGCGCGTTGCAGCAGGCCACGGGCACGCGCTTCGGTGCCGATATCGCGATCGACAAGATCCTGCC
GATGGGCGGCGGCATCGGCGGCGGCTCGTCCGACGCCGCCACGACGCTGCTCGCGCTGAACCACCTGTGGGGCCTGGGCC
TGCCGCGCGCCGAACTCATGCGGATCGGGCTGGCGCTGGGTGCCGACGTGCCCGTGTTCGTGTTCGGCGAGAACGCGTTC
GCCGAGGGCATTGGCGAGGAACTGACTGCCGTCGACTTGCCGGACAGCTGGTTCGTGATCGTCCATCCGAAACAACATGT
GCCGACCGCAGAAATTTTTTCGGACGAGTGCTTGACAAGGGACACGCCCCTCTCCATAATTGCGGTCTTCGCTGCTTGCA
CAAACAAATTCTCTTTCGGTCGCAACGACCTGGAAGCGATAGCAACAGCGAAGTTCGGCGAAGTTGCCCGAGCCCTTGAA
TGGCTGAAGCAGTTTAATCAGCATGCAAGGATGACCGGCTCCGGAGCCTGCGTGTTTGCACGTTTTCCCGACGAGCAGAA
GGCGCAGCAGGTTCTGAAGCGGTTGCCTCCCGAGTGGGATGGCAGGTGTGTGCGCAGTCTGGCACGTCACCCGCTCGCAG
CGTTCGCATAG

Upstream 100 bases:

>100_bases
CGCAAGCGCCCGCCCCGCGGCCGCGCCGCATCGACCTGGAACGCGAAGGCGGCCCCACGCCGCTGGCCGTGAAGCTCGTG
ATCGATCCGGAGGAAGCGCC

Downstream 100 bases:

>100_bases
CAAGTTATCGTTCGGCGCGGTACGGCAGTCAGGTACCACGAAGAACGGCAAGGTTGTGTAGGGGAGTCGCCAAGTTGGTC
AAGGCACCGGATTTTGATTC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MTAALTAAPTLPPPELRDCPAPAKLNLFLHVTGRRPDGYHMLQTVFQLVDWCDTLHFRRRDDGVVTRTTDIPGVPAETDL
IVRAARALQQATGTRFGADIAIDKILPMGGGIGGGSSDAATTLLALNHLWGLGLPRAELMRIGLALGADVPVFVFGENAF
AEGIGEELTAVDLPDSWFVIVHPKQHVPTAEIFSDECLTRDTPLSIIAVFAACTNKFSFGRNDLEAIATAKFGEVARALE
WLKQFNQHARMTGSGACVFARFPDEQKAQQVLKRLPPEWDGRCVRSLARHPLAAFA

Sequences:

>Translated_296_residues
MTAALTAAPTLPPPELRDCPAPAKLNLFLHVTGRRPDGYHMLQTVFQLVDWCDTLHFRRRDDGVVTRTTDIPGVPAETDL
IVRAARALQQATGTRFGADIAIDKILPMGGGIGGGSSDAATTLLALNHLWGLGLPRAELMRIGLALGADVPVFVFGENAF
AEGIGEELTAVDLPDSWFVIVHPKQHVPTAEIFSDECLTRDTPLSIIAVFAACTNKFSFGRNDLEAIATAKFGEVARALE
WLKQFNQHARMTGSGACVFARFPDEQKAQQVLKRLPPEWDGRCVRSLARHPLAAFA
>Mature_295_residues
TAALTAAPTLPPPELRDCPAPAKLNLFLHVTGRRPDGYHMLQTVFQLVDWCDTLHFRRRDDGVVTRTTDIPGVPAETDLI
VRAARALQQATGTRFGADIAIDKILPMGGGIGGGSSDAATTLLALNHLWGLGLPRAELMRIGLALGADVPVFVFGENAFA
EGIGEELTAVDLPDSWFVIVHPKQHVPTAEIFSDECLTRDTPLSIIAVFAACTNKFSFGRNDLEAIATAKFGEVARALEW
LKQFNQHARMTGSGACVFARFPDEQKAQQVLKRLPPEWDGRCVRSLARHPLAAFA

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=279, Percent_Identity=50.8960573476703, Blast_Score=243, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_CUPPJ (Q476F8)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294569.1
- ProteinModelPortal:   Q476F8
- SMR:   Q476F8
- GeneID:   3609584
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0343
- NMPDR:   fig|264198.3.peg.855
- HOGENOM:   HBG734593
- OMA:   LNLFLHV
- ProtClustDB:   PRK00343
- BioCyc:   REUT264198:REUT_A0343-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 32131; Mature: 32000

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: ACT_SITE 24-24 ACT_SITE 149-149

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAALTAAPTLPPPELRDCPAPAKLNLFLHVTGRRPDGYHMLQTVFQLVDWCDTLHFRRR
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
DDGVVTRTTDIPGVPAETDLIVRAARALQQATGTRFGADIAIDKILPMGGGIGGGSSDAA
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHH
TTLLALNHLWGLGLPRAELMRIGLALGADVPVFVFGENAFAEGIGEELTAVDLPDSWFVI
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHCCCCCCEEEECCCCCEEE
VHPKQHVPTAEIFSDECLTRDTPLSIIAVFAACTNKFSFGRNDLEAIATAKFGEVARALE
ECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
WLKQFNQHARMTGSGACVFARFPDEQKAQQVLKRLPPEWDGRCVRSLARHPLAAFA
HHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHCC
>Mature Secondary Structure 
TAALTAAPTLPPPELRDCPAPAKLNLFLHVTGRRPDGYHMLQTVFQLVDWCDTLHFRRR
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
DDGVVTRTTDIPGVPAETDLIVRAARALQQATGTRFGADIAIDKILPMGGGIGGGSSDAA
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHH
TTLLALNHLWGLGLPRAELMRIGLALGADVPVFVFGENAFAEGIGEELTAVDLPDSWFVI
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHCCCCCCEEEECCCCCEEE
VHPKQHVPTAEIFSDECLTRDTPLSIIAVFAACTNKFSFGRNDLEAIATAKFGEVARALE
ECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
WLKQFNQHARMTGSGACVFARFPDEQKAQQVLKRLPPEWDGRCVRSLARHPLAAFA
HHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA