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The map label for this gene is prs [H]

Identifier: 73540048

GI number: 73540048

Start: 370915

End: 371868

Strand: Reverse

Name: prs [H]

Synonym: Reut_A0342

Alternate gene names: 73540048

Gene position: 371868-370915 (Counterclockwise)

Preceding gene: 73540049

Following gene: 73540047

Centisome position: 9.77

GC content: 63.31

Gene sequence:

>954_bases
ATGAGCAGCGAAGGCTTGATGGTATTTACCGGCAACGCCAACCCCAAACTCGCGGAGGCTGTTGTACAGCACCTCGGCAT
TCCGCTGGGTAAGGCGCTCGTAGGCCGTTTCTCCGACGGAGAAGTTCAGGTCGAAATCCAGGAAAACGTTCGCGGCAAGC
ACGTCATCGTGTTGCAGTCCACCTGCGCGCCGACCAACGACAACCTGATGGAACTGATGGTGATGGTCGATGCGCTCAAG
CGCGCTTCGGCACGCAGCATCACTGCCGCAATGCCCTATTTCGGCTACGCCCGCCAGGACCGCCGCCCGCGTTCCGCACG
CGTGGCGATCTCGGCCAAGGTTGTGGCCAACATGCTGGAAGTCGCCGGCGTGGAGCGCGTCCTGACCATGGACCTGCACG
CTGACCAGATCCAGGGCTTCTTCGATATCCCCGTCGACAACATCTACGCATCCCCGGTACTGCTGGGCGACCTGCGCGAG
AAGAACTACGGCAACCTGCTGGTCGTGTCGCCGGACGTCGGCGGCGTGGTTCGTGCCCGTGCGCTGGCCAAGGAGCTGAA
CTGCGACCTGGCGATCATCGACAAGCGTCGTCCGAAGGCCAACGTGGCCGAAGTGATGAACATCATCGGTGAAGTCGACG
GCCGCAACTGCGTGATCATGGACGACATGATCGACACCGGCGGCACGCTGTGCAAGGCGGCCCAGGTGCTGAAGGAGCGC
GGCGCGCAGAAGGTCTTCGCCTACTGCACGCACCCGGTGCTGTCCGGCGGCGCGGCTGCCCGCATTGCGGACTCGGCGCT
CGACGAAGTGGTGGTGTGCGACACCATCCCCCTGTCGGAAGAGGCAGCCAAGTGCGGCAAGATCCGTCAGTTGTCGACCG
CTCCGCTGCTGGCCGAGACCTTCACCCGTATCGTCAAGGGCGACTCGATCATGTCGCTGTTTGCGGGTTCCTGA

Upstream 100 bases:

>100_bases
CTGCCATTACATTCCTATAGTGTCGTTACGACACTCAGTATCAGTCACCGGTTTTTGCCGGTGACGCCAAGCTCCCCCAG
CAGCAAGACAGGTGCCCCGA

Downstream 100 bases:

>100_bases
TAGAATTTAGAGCTTTGCTGCGCTGATAGCGGGTGTGTCCCACAATCAGCGTGATGTGACCGGCGCATGCATTTTACATG
CGCCACAACAAGGCTGTCTG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSSEGLMVFTGNANPKLAEAVVQHLGIPLGKALVGRFSDGEVQVEIQENVRGKHVIVLQSTCAPTNDNLMELMVMVDALK
RASARSITAAMPYFGYARQDRRPRSARVAISAKVVANMLEVAGVERVLTMDLHADQIQGFFDIPVDNIYASPVLLGDLRE
KNYGNLLVVSPDVGGVVRARALAKELNCDLAIIDKRRPKANVAEVMNIIGEVDGRNCVIMDDMIDTGGTLCKAAQVLKER
GAQKVFAYCTHPVLSGGAAARIADSALDEVVVCDTIPLSEEAAKCGKIRQLSTAPLLAETFTRIVKGDSIMSLFAGS

Sequences:

>Translated_317_residues
MSSEGLMVFTGNANPKLAEAVVQHLGIPLGKALVGRFSDGEVQVEIQENVRGKHVIVLQSTCAPTNDNLMELMVMVDALK
RASARSITAAMPYFGYARQDRRPRSARVAISAKVVANMLEVAGVERVLTMDLHADQIQGFFDIPVDNIYASPVLLGDLRE
KNYGNLLVVSPDVGGVVRARALAKELNCDLAIIDKRRPKANVAEVMNIIGEVDGRNCVIMDDMIDTGGTLCKAAQVLKER
GAQKVFAYCTHPVLSGGAAARIADSALDEVVVCDTIPLSEEAAKCGKIRQLSTAPLLAETFTRIVKGDSIMSLFAGS
>Mature_316_residues
SSEGLMVFTGNANPKLAEAVVQHLGIPLGKALVGRFSDGEVQVEIQENVRGKHVIVLQSTCAPTNDNLMELMVMVDALKR
ASARSITAAMPYFGYARQDRRPRSARVAISAKVVANMLEVAGVERVLTMDLHADQIQGFFDIPVDNIYASPVLLGDLREK
NYGNLLVVSPDVGGVVRARALAKELNCDLAIIDKRRPKANVAEVMNIIGEVDGRNCVIMDDMIDTGGTLCKAAQVLKERG
AQKVFAYCTHPVLSGGAAARIADSALDEVVVCDTIPLSEEAAKCGKIRQLSTAPLLAETFTRIVKGDSIMSLFAGS

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=313, Percent_Identity=46.3258785942492, Blast_Score=291, Evalue=7e-79,
Organism=Homo sapiens, GI84875539, Length=315, Percent_Identity=45.7142857142857, Blast_Score=289, Evalue=3e-78,
Organism=Homo sapiens, GI4506127, Length=313, Percent_Identity=46.0063897763578, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI28557709, Length=313, Percent_Identity=45.3674121405751, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI4506133, Length=346, Percent_Identity=36.4161849710983, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI194018537, Length=345, Percent_Identity=36.5217391304348, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI310128524, Length=149, Percent_Identity=37.5838926174497, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI310115209, Length=149, Percent_Identity=37.5838926174497, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI310118259, Length=149, Percent_Identity=37.5838926174497, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI310119946, Length=149, Percent_Identity=37.5838926174497, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1787458, Length=310, Percent_Identity=64.1935483870968, Blast_Score=413, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17554704, Length=310, Percent_Identity=46.1290322580645, Blast_Score=288, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17554702, Length=313, Percent_Identity=45.6869009584665, Blast_Score=288, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI25149168, Length=313, Percent_Identity=45.6869009584665, Blast_Score=288, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI71989924, Length=313, Percent_Identity=45.6869009584665, Blast_Score=287, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI17570245, Length=342, Percent_Identity=34.5029239766082, Blast_Score=209, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6320946, Length=318, Percent_Identity=42.4528301886792, Blast_Score=262, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6321776, Length=316, Percent_Identity=43.9873417721519, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6319403, Length=318, Percent_Identity=42.1383647798742, Blast_Score=256, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=43.3673469387755, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324511, Length=109, Percent_Identity=39.4495412844037, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355239, Length=314, Percent_Identity=46.1783439490446, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI45551540, Length=336, Percent_Identity=43.1547619047619, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24651458, Length=358, Percent_Identity=33.2402234636872, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24651456, Length=358, Percent_Identity=33.2402234636872, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI281362873, Length=358, Percent_Identity=33.2402234636872, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24651454, Length=358, Percent_Identity=33.2402234636872, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24651462, Length=216, Percent_Identity=37.5, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24651464, Length=216, Percent_Identity=37.5, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI45552010, Length=216, Percent_Identity=37.5, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 33995; Mature: 33864

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEGLMVFTGNANPKLAEAVVQHLGIPLGKALVGRFSDGEVQVEIQENVRGKHVIVLQS
CCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEEECCCCCCCEEEEEEC
TCAPTNDNLMELMVMVDALKRASARSITAAMPYFGYARQDRRPRSARVAISAKVVANMLE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHEEEEHHHHHHHHHH
VAGVERVLTMDLHADQIQGFFDIPVDNIYASPVLLGDLREKNYGNLLVVSPDVGGVVRAR
HHCHHHEEEEECCHHHHCCEEECCHHHEECCCEEEECCCCCCCCCEEEECCCCCHHHHHH
ALAKELNCDLAIIDKRRPKANVAEVMNIIGEVDGRNCVIMDDMIDTGGTLCKAAQVLKER
HHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHC
GAQKVFAYCTHPVLSGGAAARIADSALDEVVVCDTIPLSEEAAKCGKIRQLSTAPLLAET
CHHHHHHHHCCHHHCCCHHHHHHHHHHCCEEEECCCCCCHHHHHHCCHHHCCCCHHHHHH
FTRIVKGDSIMSLFAGS
HHHHHCCCHHHHHHCCC
>Mature Secondary Structure 
SSEGLMVFTGNANPKLAEAVVQHLGIPLGKALVGRFSDGEVQVEIQENVRGKHVIVLQS
CCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEEECCCCCCCEEEEEEC
TCAPTNDNLMELMVMVDALKRASARSITAAMPYFGYARQDRRPRSARVAISAKVVANMLE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHEEEEHHHHHHHHHH
VAGVERVLTMDLHADQIQGFFDIPVDNIYASPVLLGDLREKNYGNLLVVSPDVGGVVRAR
HHCHHHEEEEECCHHHHCCEEECCHHHEECCCEEEECCCCCCCCCEEEECCCCCHHHHHH
ALAKELNCDLAIIDKRRPKANVAEVMNIIGEVDGRNCVIMDDMIDTGGTLCKAAQVLKER
HHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHC
GAQKVFAYCTHPVLSGGAAARIADSALDEVVVCDTIPLSEEAAKCGKIRQLSTAPLLAET
CHHHHHHHHCCHHHCCCHHHHHHHHHHCCEEEECCCCCCHHHHHHCCHHHCCCCHHHHHH
FTRIVKGDSIMSLFAGS
HHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]