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The map label for this gene is moeB [H]

Identifier: 73540008

GI number: 73540008

Start: 334338

End: 335087

Strand: Reverse

Name: moeB [H]

Synonym: Reut_A0302

Alternate gene names: 73540008

Gene position: 335087-334338 (Counterclockwise)

Preceding gene: 73540009

Following gene: 73539995

Centisome position: 8.8

GC content: 66.67

Gene sequence:

>750_bases
ATGAACGACGATCAACTGCTGCGCTATTCGCGCCATATCCTGCTCGATGAACTCGGCATCGAAGGCCAGGAGCGCCTGCT
GGGTGCCCATGCGCTGGTGATTGGCGCGGGCGGCCTGGGCGCCGCCGCCCTGCCCTATCTGGCGTCCGCCGGCGTCGGCC
GGATCAGCGTGGTCGACAACGACCATGTGGACCTGACCAATCTCCAGAGACAGATCATCCATACCACGGACAATGTCGGG
CGCCTGAAGGTGGAGTCCGCGCGTGAGGGCATGCTGCGGATCAATCCGGGTGTGCAGGTTGATGTCATCGCCGCTCGCGT
CGGTGATGCCGATCTGGAACAGCTCGTGGCACAGGCCGACGTGGTACTCGACTGCTGCGACAACTTCGCCACGCGCCAGG
CGGTCAACCGCGCCTGCGTGCATCACCGCGTACCGCTGGTCTCCGGGGCGGCGCTGCGTTTCGACGGGCAGATCAGCGTC
TTCGACCGGCGCCAGGACAACGCACCGTGCTACGCATGCCTGTTCCCGCCGTCAGAGCCCGCACCGGAAGTCGCCTGCGC
GACCATGGGCGTGTTCGCGCCGCTGGTCGGCATGGTTGGTACCGTGCAGGCGGCCGAGGCGCTCAAACTGCTCAGTGGCG
TGGGCCAGAGCCTGGCCGGCCGGCTGCTGATGGTCAATGCGATGACGATGGAGTGGACCACGATGCAGCTCGCGCGCACA
CCCGACTGCCCGGTTTGCGCCGGGCATTGA

Upstream 100 bases:

>100_bases
GGGCACGCCGACCGGCAAGGCGCCCTGAGCGGCACCCTGACCAGAAGCGGCCCTGCGGGGCCGCTTTTTACATTGCACGT
CCTGTCCTGAGCCTCATGCC

Downstream 100 bases:

>100_bases
CAGGCATCAGCGTGCCGAGCGCTCGCGCAGCCAGTCGGTGACCGCCGCGCGGTCGCCGCGGAACACCACACGCGCGGCGC
GTTTCTCTTCCTGGTACGCG

Product: molybdopterin biosynthesis protein MoeB

Products: NA

Alternate protein names: MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNDDQLLRYSRHILLDELGIEGQERLLGAHALVIGAGGLGAAALPYLASAGVGRISVVDNDHVDLTNLQRQIIHTTDNVG
RLKVESAREGMLRINPGVQVDVIAARVGDADLEQLVAQADVVLDCCDNFATRQAVNRACVHHRVPLVSGAALRFDGQISV
FDRRQDNAPCYACLFPPSEPAPEVACATMGVFAPLVGMVGTVQAAEALKLLSGVGQSLAGRLLMVNAMTMEWTTMQLART
PDCPVCAGH

Sequences:

>Translated_249_residues
MNDDQLLRYSRHILLDELGIEGQERLLGAHALVIGAGGLGAAALPYLASAGVGRISVVDNDHVDLTNLQRQIIHTTDNVG
RLKVESAREGMLRINPGVQVDVIAARVGDADLEQLVAQADVVLDCCDNFATRQAVNRACVHHRVPLVSGAALRFDGQISV
FDRRQDNAPCYACLFPPSEPAPEVACATMGVFAPLVGMVGTVQAAEALKLLSGVGQSLAGRLLMVNAMTMEWTTMQLART
PDCPVCAGH
>Mature_249_residues
MNDDQLLRYSRHILLDELGIEGQERLLGAHALVIGAGGLGAAALPYLASAGVGRISVVDNDHVDLTNLQRQIIHTTDNVG
RLKVESAREGMLRINPGVQVDVIAARVGDADLEQLVAQADVVLDCCDNFATRQAVNRACVHHRVPLVSGAALRFDGQISV
FDRRQDNAPCYACLFPPSEPAPEVACATMGVFAPLVGMVGTVQAAEALKLLSGVGQSLAGRLLMVNAMTMEWTTMQLART
PDCPVCAGH

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=229, Percent_Identity=44.5414847161572, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI4885649, Length=157, Percent_Identity=30.5732484076433, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI23510338, Length=153, Percent_Identity=30.0653594771242, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI23510340, Length=153, Percent_Identity=30.0653594771242, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI38045948, Length=154, Percent_Identity=31.1688311688312, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1787048, Length=248, Percent_Identity=47.5806451612903, Blast_Score=231, Evalue=4e-62,
Organism=Escherichia coli, GI87082356, Length=249, Percent_Identity=44.578313253012, Blast_Score=203, Evalue=8e-54,
Organism=Escherichia coli, GI1789177, Length=138, Percent_Identity=36.231884057971, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540406, Length=258, Percent_Identity=40.3100775193798, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI193203301, Length=194, Percent_Identity=29.3814432989691, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI86565433, Length=147, Percent_Identity=29.2517006802721, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI86565431, Length=147, Percent_Identity=29.2517006802721, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17508819, Length=182, Percent_Identity=30.2197802197802, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=252, Percent_Identity=34.9206349206349, Blast_Score=162, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6322639, Length=168, Percent_Identity=27.9761904761905, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320598, Length=141, Percent_Identity=28.3687943262411, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24582879, Length=251, Percent_Identity=38.2470119521912, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24660640, Length=173, Percent_Identity=33.5260115606936, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI28573937, Length=162, Percent_Identity=30.2469135802469, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR012730
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 26486; Mature: 26486

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDDQLLRYSRHILLDELGIEGQERLLGAHALVIGAGGLGAAALPYLASAGVGRISVVDN
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHEEEEEECCCCCHHHHHHHHHCCCCEEEEECC
DHVDLTNLQRQIIHTTDNVGRLKVESAREGMLRINPGVQVDVIAARVGDADLEQLVAQAD
CCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEECCCCEEEEEEEECCCHHHHHHHHHHH
VVLDCCDNFATRQAVNRACVHHRVPLVSGAALRFDGQISVFDRRQDNAPCYACLFPPSEP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCCEEEEECCCCCC
APEVACATMGVFAPLVGMVGTVQAAEALKLLSGVGQSLAGRLLMVNAMTMEWTTMQLART
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCEEEEEEEEEEEEEEEEEECC
PDCPVCAGH
CCCCCCCCC
>Mature Secondary Structure
MNDDQLLRYSRHILLDELGIEGQERLLGAHALVIGAGGLGAAALPYLASAGVGRISVVDN
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHEEEEEECCCCCHHHHHHHHHCCCCEEEEECC
DHVDLTNLQRQIIHTTDNVGRLKVESAREGMLRINPGVQVDVIAARVGDADLEQLVAQAD
CCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEECCCCEEEEEEEECCCHHHHHHHHHHH
VVLDCCDNFATRQAVNRACVHHRVPLVSGAALRFDGQISVFDRRQDNAPCYACLFPPSEP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCCEEEEECCCCCC
APEVACATMGVFAPLVGMVGTVQAAEALKLLSGVGQSLAGRLLMVNAMTMEWTTMQLART
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCEEEEEEEEEEEEEEEEEECC
PDCPVCAGH
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3045084; 8905232; 9278503 [H]