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The map label for this gene is rhtB [H]

Identifier: 73539993

GI number: 73539993

Start: 319646

End: 320290

Strand: Direct

Name: rhtB [H]

Synonym: Reut_A0287

Alternate gene names: 73539993

Gene position: 319646-320290 (Clockwise)

Preceding gene: 73539992

Following gene: 73539996

Centisome position: 8.4

GC content: 68.53

Gene sequence:

>645_bases
ATGCGTTGGGAAGTCTGGCTGGCCTATTTCGCCGCGTGCTGGGTCATTGCGGTGTCGCCCGGTTCGGGCGCGGTGCTGTC
GATGAGCCACGGCCTGTCGTACGGCGTGCGTCGGACCTCGACCACGATCTTCGGCCTGCAGGCGGGGCTGGTGATCATCC
TGCTCGTGGCCGGCGGCGGGCTCGGCGCGCTGCTGCTCGCGTCGGAGCATGCGTTTGCCGTGGTCAAGACCGTCGGCGCG
ATGTACCTGATCTATCTGGGCCTGCAGCAATGGCGAGCGAGGGTCGAGGAGGGCGGCACCGACAGCGGCAGCACCGCTGT
CCGCGTGACGCCCATGAGCCCGCGCCGGCGCTTCGCCACGGGGCTGCTGACCAATGTGACCAACCCGAAGGGCATCATCT
TCATGGTCGCCGTGCTGCCGCAGTTCATCGACCCGTCCCGCCCGCTGGCACCGCAGCTCGCCATCCTGGCGGCCACCATG
TGCGGCGTGGACCTGATCGTCATGCACGGCTACGCGTTGCTGGCCTCGCGCATGCAGGGCCTGTTCCGTAACGCGCGTGC
CGTGCGCTGGCAGAACCGCTTCTTCGGCAGCGTGTTGATGGCGGTAGGCGCGGCGCTGTTCTTCGTGCGTCGGCAGCACG
CCTGA

Upstream 100 bases:

>100_bases
GCGCGCGCGACCAGCGCAACGCCGAGCAGGTGGAGCAGTTCCTGGGCGGATTCCATCCGTATTGATCCACCCGGCAAGAT
TTATTCGAAGGAGAGAAGTG

Downstream 100 bases:

>100_bases
TAGCCGCATCCCGGTTGCGTGCTGGCGGCCTGACGCCCGGTTTGCTTCAATGGGAGAGACCCCTCGCCGCGGAGATCTCC
CGAATGCCGACCATTCCGTT

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MRWEVWLAYFAACWVIAVSPGSGAVLSMSHGLSYGVRRTSTTIFGLQAGLVIILLVAGGGLGALLLASEHAFAVVKTVGA
MYLIYLGLQQWRARVEEGGTDSGSTAVRVTPMSPRRRFATGLLTNVTNPKGIIFMVAVLPQFIDPSRPLAPQLAILAATM
CGVDLIVMHGYALLASRMQGLFRNARAVRWQNRFFGSVLMAVGAALFFVRRQHA

Sequences:

>Translated_214_residues
MRWEVWLAYFAACWVIAVSPGSGAVLSMSHGLSYGVRRTSTTIFGLQAGLVIILLVAGGGLGALLLASEHAFAVVKTVGA
MYLIYLGLQQWRARVEEGGTDSGSTAVRVTPMSPRRRFATGLLTNVTNPKGIIFMVAVLPQFIDPSRPLAPQLAILAATM
CGVDLIVMHGYALLASRMQGLFRNARAVRWQNRFFGSVLMAVGAALFFVRRQHA
>Mature_214_residues
MRWEVWLAYFAACWVIAVSPGSGAVLSMSHGLSYGVRRTSTTIFGLQAGLVIILLVAGGGLGALLLASEHAFAVVKTVGA
MYLIYLGLQQWRARVEEGGTDSGSTAVRVTPMSPRRRFATGLLTNVTNPKGIIFMVAVLPQFIDPSRPLAPQLAILAATM
CGVDLIVMHGYALLASRMQGLFRNARAVRWQNRFFGSVLMAVGAALFFVRRQHA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=206, Percent_Identity=42.7184466019417, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI1788099, Length=168, Percent_Identity=25.5952380952381, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23096; Mature: 23096

Theoretical pI: Translated: 11.70; Mature: 11.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWEVWLAYFAACWVIAVSPGSGAVLSMSHGLSYGVRRTSTTIFGLQAGLVIILLVAGGG
CCHHHHHHHHHHHHEEEECCCCCEEEEECCCCHHCCHHHHHHHHHHHHHHHEEEEECCCC
LGALLLASEHAFAVVKTVGAMYLIYLGLQQWRARVEEGGTDSGSTAVRVTPMSPRRRFAT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHH
GLLTNVTNPKGIIFMVAVLPQFIDPSRPLAPQLAILAATMCGVDLIVMHGYALLASRMQG
HHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFRNARAVRWQNRFFGSVLMAVGAALFFVRRQHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRWEVWLAYFAACWVIAVSPGSGAVLSMSHGLSYGVRRTSTTIFGLQAGLVIILLVAGGG
CCHHHHHHHHHHHHEEEECCCCCEEEEECCCCHHCCHHHHHHHHHHHHHHHEEEEECCCC
LGALLLASEHAFAVVKTVGAMYLIYLGLQQWRARVEEGGTDSGSTAVRVTPMSPRRRFAT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHH
GLLTNVTNPKGIIFMVAVLPQFIDPSRPLAPQLAILAATMCGVDLIVMHGYALLASRMQG
HHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFRNARAVRWQNRFFGSVLMAVGAALFFVRRQHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]