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The map label for this gene is rlmI [H]

Identifier: 73539988

GI number: 73539988

Start: 314208

End: 315404

Strand: Direct

Name: rlmI [H]

Synonym: Reut_A0282

Alternate gene names: 73539988

Gene position: 314208-315404 (Clockwise)

Preceding gene: 73539987

Following gene: 73539992

Centisome position: 8.25

GC content: 66.67

Gene sequence:

>1197_bases
ATGAACATCATCACCCTCAAGCCCGGCAAGGAAAAATCCCTGCTGCGCCGCCACCCGTGGATCTACGCGACGGGCATTGC
GACCACGGAAGGCCGCTGCGAGCCGGGCGCCACCGTCATCGTGCGCGCCGCCGATGGCCGCTTCCTGGCCAAGGCCGCCT
ACAGCCCCGAATCGCAGATCCGCGCACGCGTGTGGACCTTCGACGAGAACGAGCCCGTCGACCACGCCATGTTCAAGCGC
CGCATCTCGGCGGCGGTGGCGCATCGCCGTCAGTGGGTGAAGGACAGCGACGCGGTACGGCTGATCTTCGGTGAATCCGA
CCGCCTGCCGGGCCTGATCGTCGACTACTACGGCAATGGCGAAAGCGGCCAGCTCGTGTGCCAGTTCAACGCCGCGGGCG
TGGAGCATTGGAAGACCGCCATCGTGCAGGCGCTGGTCAAGGAGACCGGCTGCCCGAACGTGTACGAGCGGTCCGACGCC
GCCGTGCGCCAGCGCGAGGGACTGGAACTCGTGACCGGCGTGCTGGCCGGTGCTGAACCTGACCCGGCGCTGTCGGTGAC
CGAGCATGGCGTGCGTTACTACGTCGACGTGCGCAACGGCCACAAGACTGGCTTCTATGTCGACCAGCGCGACAACCGCA
AACTCGTCGGCGATCTCGCGGCGGGCCGCGAGGTGCTCAACTGCTTCTGCTACACGGGCGGCTTCTCGCTGGCCGCACTG
CGCGGTGGCGCCACGTCGGTGACGTCGATCGATTCCTCGGGCGAGGCGCTGAAGATCGCCGCCGGCAATGTCACGCTCAA
CGGCTTCGATGAGAACCGCGCGACGTGGCTCGACGCCGACGTGTTCAAGACGCTGCGCGAATTCCGCGCGGAGGGCCGCC
AGTTTGACCTGATCGTGCTCGACCCGCCGAAGTTCGCGCCGTCGTCGCAGCATATCGACCGCGCTGCGCGTGCCTACAAG
GAAATCAACCTGGTCGGCATGCAGTTGCTGCGTCCGGGCGGGCTGCTGTTCACGTACTCGTGCTCGGGTGCGATCAGCAT
GGAGCTGTTCCAGAAGATCGTGGCCGGTGCGGTGACGGATGCGCGCGCTGATGCGCGCATCATGCGGCGTCTGTCGGCCG
GCACGGATCACCCGATGCTGGCGGCGTTCCCGGAAGGGGAGTACCTGAAGGGGTTGCTGCTGGAGAAGGTGGCGTAA

Upstream 100 bases:

>100_bases
CTCCGCCCCTCACCCCCAACCCCTCTCCCGTACACGGAGAGGGGGCCGCGCCGCAGTGTGAGAAACGCTTCTGGCGTGGC
GAGCTTTTCCCCATACCAAC

Downstream 100 bases:

>100_bases
AGGGATACGGCGGAAGCGTGAGGCTCACCGCCACATATTCCGCATCCTGCTGGCCAGGTCCACCGGCGCCAGCGTAGCCT
TCGACGGCGCCTCCCCCGGC

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNIITLKPGKEKSLLRRHPWIYATGIATTEGRCEPGATVIVRAADGRFLAKAAYSPESQIRARVWTFDENEPVDHAMFKR
RISAAVAHRRQWVKDSDAVRLIFGESDRLPGLIVDYYGNGESGQLVCQFNAAGVEHWKTAIVQALVKETGCPNVYERSDA
AVRQREGLELVTGVLAGAEPDPALSVTEHGVRYYVDVRNGHKTGFYVDQRDNRKLVGDLAAGREVLNCFCYTGGFSLAAL
RGGATSVTSIDSSGEALKIAAGNVTLNGFDENRATWLDADVFKTLREFRAEGRQFDLIVLDPPKFAPSSQHIDRAARAYK
EINLVGMQLLRPGGLLFTYSCSGAISMELFQKIVAGAVTDARADARIMRRLSAGTDHPMLAAFPEGEYLKGLLLEKVA

Sequences:

>Translated_398_residues
MNIITLKPGKEKSLLRRHPWIYATGIATTEGRCEPGATVIVRAADGRFLAKAAYSPESQIRARVWTFDENEPVDHAMFKR
RISAAVAHRRQWVKDSDAVRLIFGESDRLPGLIVDYYGNGESGQLVCQFNAAGVEHWKTAIVQALVKETGCPNVYERSDA
AVRQREGLELVTGVLAGAEPDPALSVTEHGVRYYVDVRNGHKTGFYVDQRDNRKLVGDLAAGREVLNCFCYTGGFSLAAL
RGGATSVTSIDSSGEALKIAAGNVTLNGFDENRATWLDADVFKTLREFRAEGRQFDLIVLDPPKFAPSSQHIDRAARAYK
EINLVGMQLLRPGGLLFTYSCSGAISMELFQKIVAGAVTDARADARIMRRLSAGTDHPMLAAFPEGEYLKGLLLEKVA
>Mature_398_residues
MNIITLKPGKEKSLLRRHPWIYATGIATTEGRCEPGATVIVRAADGRFLAKAAYSPESQIRARVWTFDENEPVDHAMFKR
RISAAVAHRRQWVKDSDAVRLIFGESDRLPGLIVDYYGNGESGQLVCQFNAAGVEHWKTAIVQALVKETGCPNVYERSDA
AVRQREGLELVTGVLAGAEPDPALSVTEHGVRYYVDVRNGHKTGFYVDQRDNRKLVGDLAAGREVLNCFCYTGGFSLAAL
RGGATSVTSIDSSGEALKIAAGNVTLNGFDENRATWLDADVFKTLREFRAEGRQFDLIVLDPPKFAPSSQHIDRAARAYK
EINLVGMQLLRPGGLLFTYSCSGAISMELFQKIVAGAVTDARADARIMRRLSAGTDHPMLAAFPEGEYLKGLLLEKVA

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=392, Percent_Identity=46.4285714285714, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1787181, Length=168, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43622; Mature: 43622

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIITLKPGKEKSLLRRHPWIYATGIATTEGRCEPGATVIVRAADGRFLAKAAYSPESQI
CEEEEECCCCCHHHHHHCCCEEEECEECCCCCCCCCCEEEEEECCCCEEEEECCCCHHHE
RARVWTFDENEPVDHAMFKRRISAAVAHRRQWVKDSDAVRLIFGESDRLPGLIVDYYGNG
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCC
ESGQLVCQFNAAGVEHWKTAIVQALVKETGCPNVYERSDAAVRQREGLELVTGVLAGAEP
CCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHCCHHHHHHHHCCCCC
DPALSVTEHGVRYYVDVRNGHKTGFYVDQRDNRKLVGDLAAGREVLNCFCYTGGFSLAAL
CCCCEEECCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHEECCCCEEEEE
RGGATSVTSIDSSGEALKIAAGNVTLNGFDENRATWLDADVFKTLREFRAEGRQFDLIVL
CCCCCEEEEECCCCCEEEEEECEEEEECCCCCCCEEECHHHHHHHHHHHHCCCEEEEEEE
DPPKFAPSSQHIDRAARAYKEINLVGMQLLRPGGLLFTYSCSGAISMELFQKIVAGAVTD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHC
ARADARIMRRLSAGTDHPMLAAFPEGEYLKGLLLEKVA
HHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNIITLKPGKEKSLLRRHPWIYATGIATTEGRCEPGATVIVRAADGRFLAKAAYSPESQI
CEEEEECCCCCHHHHHHCCCEEEECEECCCCCCCCCCEEEEEECCCCEEEEECCCCHHHE
RARVWTFDENEPVDHAMFKRRISAAVAHRRQWVKDSDAVRLIFGESDRLPGLIVDYYGNG
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCC
ESGQLVCQFNAAGVEHWKTAIVQALVKETGCPNVYERSDAAVRQREGLELVTGVLAGAEP
CCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHCCHHHHHHHHCCCCC
DPALSVTEHGVRYYVDVRNGHKTGFYVDQRDNRKLVGDLAAGREVLNCFCYTGGFSLAAL
CCCCEEECCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHEECCCCEEEEE
RGGATSVTSIDSSGEALKIAAGNVTLNGFDENRATWLDADVFKTLREFRAEGRQFDLIVL
CCCCCEEEEECCCCCEEEEEECEEEEECCCCCCCEEECHHHHHHHHHHHHCCCEEEEEEE
DPPKFAPSSQHIDRAARAYKEINLVGMQLLRPGGLLFTYSCSGAISMELFQKIVAGAVTD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHC
ARADARIMRRLSAGTDHPMLAAFPEGEYLKGLLLEKVA
HHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA