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The map label for this gene is ttcA [H]

Identifier: 73539933

GI number: 73539933

Start: 252297

End: 253235

Strand: Direct

Name: ttcA [H]

Synonym: Reut_A0227

Alternate gene names: 73539933

Gene position: 252297-253235 (Clockwise)

Preceding gene: 73539932

Following gene: 73539935

Centisome position: 6.63

GC content: 62.41

Gene sequence:

>939_bases
ATGAGCCACTCCAATAACTTCTACCGCCTCGAAACGAGGCTGCAATCGCAAACCGGCAAGGCCATCGGCGACTTCGGCAT
GATCGAGGACGGCGACACCGTGCTGGTCTGCATGAGTGGCGGCAAAGACTCCTACACCATGCTGTCGGTCCTGATGGCGC
TGCAGAAGCGCGCGCCCATCCAGTTCAAGCTGATTGCGATGAACCTGGACCAGAAGCAACCCGGCTTCCCCGAGCATGTG
CTGCCGGAGTACCTGAAGTCGACGGGCGTGGAATACGTGATCGTCGAGGCGGACACCTACTCGATCGTCAAGGAGAAGGT
GCCCGAGGGCAAGACCACGTGCTCGCTGTGCTCGCGTCTGCGCCGCGGCGTGATCTACCGCACCGCCAAGGAGCTTGGCG
CCAACAAGATCGCGCTGGGCCACCACCGCGACGACATCGTCAACACGTTCTTCCTGAACATGTTCTTCGGCGGCAAGATG
AAGGCCATGCCGCCCAAGCTCGCCACGGACGACGGCGCGCACATCGTGATCCGGCCGCTCGCGTATTGCTCGGAGAAGGA
CATCGCCGCGTATGCGCGCGCGATGGAATTCCCGATCATCCCGTGCAACCTGTGCGGCTCGCAGGAGAACCTGCAGCGCA
AGAAGGTCAGTGAAATGCTGCAGGCATGGGAGCGCGAGAACCCGGGCCGTATCGACAATATCTTCGCGTCGCTGCGCAAT
GTGGTGCCGTCGCACCTGGCTGATACTGACCTGTTCCCGTTCACGGGGCTGGCCACGGGCTTGGCCAAGGTGGACGAGGC
CTCCCTGTTCGGCGAGACCACGTTCACGCAGCAGCCGCTGGTGTTTGCGGGCAGCAGCGACGAGAACCGGCTGGAATTTG
TGCGTTTCGAACGTCCGCCACAGACGTCGGCGACCGTCCAGCAGGCCAGCATCGAATAA

Upstream 100 bases:

>100_bases
TCCACATCAAGCAGAACTGAGCCGGCCCGAGTAAAATCTTGGCCCTTCCGAGCCATCCCGGCCATTTCATCCGGGCGGCC
GCAACGATCAGGTATGTCTC

Downstream 100 bases:

>100_bases
GCGGCCCGCCGTCAGACAGAACCCCGCGTCGTGACGCGGGCGTTTTGTTTGGGTTCGGCTTACTTGTTGGCCGCCTGGGA
CGTGTTGCCCTGCCGCTCGC

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSHSNNFYRLETRLQSQTGKAIGDFGMIEDGDTVLVCMSGGKDSYTMLSVLMALQKRAPIQFKLIAMNLDQKQPGFPEHV
LPEYLKSTGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRLRRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKM
KAMPPKLATDDGAHIVIRPLAYCSEKDIAAYARAMEFPIIPCNLCGSQENLQRKKVSEMLQAWERENPGRIDNIFASLRN
VVPSHLADTDLFPFTGLATGLAKVDEASLFGETTFTQQPLVFAGSSDENRLEFVRFERPPQTSATVQQASIE

Sequences:

>Translated_312_residues
MSHSNNFYRLETRLQSQTGKAIGDFGMIEDGDTVLVCMSGGKDSYTMLSVLMALQKRAPIQFKLIAMNLDQKQPGFPEHV
LPEYLKSTGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRLRRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKM
KAMPPKLATDDGAHIVIRPLAYCSEKDIAAYARAMEFPIIPCNLCGSQENLQRKKVSEMLQAWERENPGRIDNIFASLRN
VVPSHLADTDLFPFTGLATGLAKVDEASLFGETTFTQQPLVFAGSSDENRLEFVRFERPPQTSATVQQASIE
>Mature_311_residues
SHSNNFYRLETRLQSQTGKAIGDFGMIEDGDTVLVCMSGGKDSYTMLSVLMALQKRAPIQFKLIAMNLDQKQPGFPEHVL
PEYLKSTGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRLRRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKMK
AMPPKLATDDGAHIVIRPLAYCSEKDIAAYARAMEFPIIPCNLCGSQENLQRKKVSEMLQAWERENPGRIDNIFASLRNV
VPSHLADTDLFPFTGLATGLAKVDEASLFGETTFTQQPLVFAGSSDENRLEFVRFERPPQTSATVQQASIE

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=292, Percent_Identity=63.3561643835616, Blast_Score=404, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI193211090, Length=235, Percent_Identity=33.6170212765957, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17540090, Length=195, Percent_Identity=27.1794871794872, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24651972, Length=235, Percent_Identity=25.9574468085106, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 34742; Mature: 34611

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSNNFYRLETRLQSQTGKAIGDFGMIEDGDTVLVCMSGGKDSYTMLSVLMALQKRAPI
CCCCCCEEEEHHHHHHHCCCHHCCCCCEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
QFKLIAMNLDQKQPGFPEHVLPEYLKSTGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRL
EEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCCCHHHHHHHHH
RRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKMKAMPPKLATDDGAHIVIRPL
HHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEH
AYCSEKDIAAYARAMEFPIIPCNLCGSQENLQRKKVSEMLQAWERENPGRIDNIFASLRN
HHCCCCHHHHHHHHHCCCCEEHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VVPSHLADTDLFPFTGLATGLAKVDEASLFGETTFTQQPLVFAGSSDENRLEFVRFERPP
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEECCCC
QTSATVQQASIE
CCCHHHHHHCCC
>Mature Secondary Structure 
SHSNNFYRLETRLQSQTGKAIGDFGMIEDGDTVLVCMSGGKDSYTMLSVLMALQKRAPI
CCCCCEEEEHHHHHHHCCCHHCCCCCEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
QFKLIAMNLDQKQPGFPEHVLPEYLKSTGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRL
EEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCCCHHHHHHHHH
RRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKMKAMPPKLATDDGAHIVIRPL
HHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEH
AYCSEKDIAAYARAMEFPIIPCNLCGSQENLQRKKVSEMLQAWERENPGRIDNIFASLRN
HHCCCCHHHHHHHHHCCCCEEHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VVPSHLADTDLFPFTGLATGLAKVDEASLFGETTFTQQPLVFAGSSDENRLEFVRFERPP
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEECCCC
QTSATVQQASIE
CCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA