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The map label for this gene is glmU

Identifier: 73539935

GI number: 73539935

Start: 254434

End: 255798

Strand: Direct

Name: glmU

Synonym: Reut_A0229

Alternate gene names: 73539935

Gene position: 254434-255798 (Clockwise)

Preceding gene: 73539933

Following gene: 73539936

Centisome position: 6.68

GC content: 65.27

Gene sequence:

>1365_bases
GTGAATATCGTCATTCTCGCCGCGGGCATGGGCAAGCGGATGCATTCCGATTTGCCAAAGGTCCTGCACCCTGTTGCCGG
GCGCCCGATGCTCGCGCATGTCATCGATACGGCGCGTACGCTGTCGCCTTCGCGGCTCGTGGTGGTCATTGGCCACGGCG
CGGAGCGCGTGCGCGAAGCCGTCGCCGCCGATGACGTCACCTTTGCCGAACAGGACCAGCAACTGGGTACCGGCCATGCG
GTGATGCAGGCACTTCCTCAGTTGGACGACAACCAGCCCACATTGGTTCTCTATGGTGACGTGCCGCTCACCACGGCAGC
CACCCTCAAGGCGCTGGTCGAGGCAGCCGGCAACACGCGTTTCGGCGTGCTGACCGTGGAAATGCCCGATCCGACTGGCT
ACGGGCGCATCGTGCGCGATGCCGCGGGCAGCATCGTACGGATCGTCGAGCAGAAGGACGCCAGCGAGGCCGTCCGCGCG
ATCCGCGAGATCAACACCGGCATCATCGTGTGCCCGACAGGCCATCTGCGCCGCTGGCTGGCCACGCTGCGCAACGACAA
TGCCCAGGGCGAGTACTACCTGACCGATACCGTCGAGCGCGCGGCGACCGAAGGCATCGACATTGTCTCGGCCCAGCCTG
CGGCGCTGTGGGAGACGCTCGGCGTCAACAGCAAGGTGCAACTTGCCGAAGTGGAACGCATCCACCAGCGCAATCTGGCG
CAGCGCCTGCTCGAAACTGGCGTGACGCTGGCCGATCCGGCGCGCATCGACGTACGCGGCGAGCTGACCTGCGGCCGCGA
TGTTTCCATCGACATCGGCTGCGTGTTCGAAGGCCGCGTGCATCTCGGCGACGGCGTGCAGATCGGCGCCAATTGCGTCA
TTCGCAACAGCAGCATCGATGCCGGCGCGCAGGTGCAGCCGTTCTGCCATATCGACAGCGCGAAGATCGGCGCGGACGGC
CGCATCGGCCCGTATGCGCGCCTGCGCCCCGGCACCGAACTGGGCGAAGATGTCCATATCGGCAACTTTGTCGAAGTGAA
GAATTCGCAGGTCGCCGCGCACAGCAAGGCCAATCATCTGGCTTATGTGGGCGACGCCACGGTCGGCGCGCGCGTGAACA
TTGGCGCCGGCACCATCACCTGCAACTATGACGGTGCGAACAAGTTCCGCACGGTGATCGAGGACGACGTTTTTATCGGC
TCCGATACGCAACTGGTTGCGCCGGTCACGGTGCGCCGCGGTGCGACTATCGGCGCAGGCACGACGCTGACCAAGGAGGC
GCCGGCCGACAAGCTGACGCTGTCGCGCGCCAAGCAGATGACGCTCGACGCCTGGCAGCGCCCAGTCAAGAAGGCGAAGC
AGTAA

Upstream 100 bases:

>100_bases
GCGGAAGCCGGGTTGTCGAACGCCGTGACGCCGGTTATCGAACCTCCGGCTGGTGCTTTAAAGAATATATTTCCCGATAC
ACGCACCAAGGGGTCTCCTT

Downstream 100 bases:

>100_bases
GCGGTATGGCCGCCTGCATGTCGTGTACGGTGGCCATCGATCGACACAATACGACGGACGCAGTGTTGCGGCCGTTCCGG
CGCGCCGCTGTGGCTGCGCA

Product: UDP-N-acetylglucosamine pyrophosphorylase / glucosamine-1-phosphate N-acetyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MNIVILAAGMGKRMHSDLPKVLHPVAGRPMLAHVIDTARTLSPSRLVVVIGHGAERVREAVAADDVTFAEQDQQLGTGHA
VMQALPQLDDNQPTLVLYGDVPLTTAATLKALVEAAGNTRFGVLTVEMPDPTGYGRIVRDAAGSIVRIVEQKDASEAVRA
IREINTGIIVCPTGHLRRWLATLRNDNAQGEYYLTDTVERAATEGIDIVSAQPAALWETLGVNSKVQLAEVERIHQRNLA
QRLLETGVTLADPARIDVRGELTCGRDVSIDIGCVFEGRVHLGDGVQIGANCVIRNSSIDAGAQVQPFCHIDSAKIGADG
RIGPYARLRPGTELGEDVHIGNFVEVKNSQVAAHSKANHLAYVGDATVGARVNIGAGTITCNYDGANKFRTVIEDDVFIG
SDTQLVAPVTVRRGATIGAGTTLTKEAPADKLTLSRAKQMTLDAWQRPVKKAKQ

Sequences:

>Translated_454_residues
MNIVILAAGMGKRMHSDLPKVLHPVAGRPMLAHVIDTARTLSPSRLVVVIGHGAERVREAVAADDVTFAEQDQQLGTGHA
VMQALPQLDDNQPTLVLYGDVPLTTAATLKALVEAAGNTRFGVLTVEMPDPTGYGRIVRDAAGSIVRIVEQKDASEAVRA
IREINTGIIVCPTGHLRRWLATLRNDNAQGEYYLTDTVERAATEGIDIVSAQPAALWETLGVNSKVQLAEVERIHQRNLA
QRLLETGVTLADPARIDVRGELTCGRDVSIDIGCVFEGRVHLGDGVQIGANCVIRNSSIDAGAQVQPFCHIDSAKIGADG
RIGPYARLRPGTELGEDVHIGNFVEVKNSQVAAHSKANHLAYVGDATVGARVNIGAGTITCNYDGANKFRTVIEDDVFIG
SDTQLVAPVTVRRGATIGAGTTLTKEAPADKLTLSRAKQMTLDAWQRPVKKAKQ
>Mature_454_residues
MNIVILAAGMGKRMHSDLPKVLHPVAGRPMLAHVIDTARTLSPSRLVVVIGHGAERVREAVAADDVTFAEQDQQLGTGHA
VMQALPQLDDNQPTLVLYGDVPLTTAATLKALVEAAGNTRFGVLTVEMPDPTGYGRIVRDAAGSIVRIVEQKDASEAVRA
IREINTGIIVCPTGHLRRWLATLRNDNAQGEYYLTDTVERAATEGIDIVSAQPAALWETLGVNSKVQLAEVERIHQRNLA
QRLLETGVTLADPARIDVRGELTCGRDVSIDIGCVFEGRVHLGDGVQIGANCVIRNSSIDAGAQVQPFCHIDSAKIGADG
RIGPYARLRPGTELGEDVHIGNFVEVKNSQVAAHSKANHLAYVGDATVGARVNIGAGTITCNYDGANKFRTVIEDDVFIG
SDTQLVAPVTVRRGATIGAGTTLTKEAPADKLTLSRAKQMTLDAWQRPVKKAKQ

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=451, Percent_Identity=52.7716186252772, Blast_Score=482, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_CUPPJ (Q476S2)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294455.1
- ProteinModelPortal:   Q476S2
- SMR:   Q476S2
- GeneID:   3610349
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0229
- NMPDR:   fig|264198.3.peg.741
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- ProtClustDB:   CLSK896503
- BioCyc:   REUT264198:REUT_A0229-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 48503; Mature: 48503

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 359-359 BINDING 71-71 BINDING 135-135 BINDING 150-150 BINDING 165-165 BINDING 383-383 BINDING 401-401 BINDING 419-419 BINDING 436-436

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVILAAGMGKRMHSDLPKVLHPVAGRPMLAHVIDTARTLSPSRLVVVIGHGAERVREA
CEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHH
VAADDVTFAEQDQQLGTGHAVMQALPQLDDNQPTLVLYGDVPLTTAATLKALVEAAGNTR
HHHCCCEEHHCHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCE
FGVLTVEMPDPTGYGRIVRDAAGSIVRIVEQKDASEAVRAIREINTGIIVCPTGHLRRWL
EEEEEEECCCCCCCCHHHHHHCCCEEEEHHHCCHHHHHHHHHHCCCCEEEECCHHHHHHH
ATLRNDNAQGEYYLTDTVERAATEGIDIVSAQPAALWETLGVNSKVQLAEVERIHQRNLA
HHHHCCCCCCCEEEEHHHHHHHHCCCEEECCCCHHHHHHHCCCCCEEHHHHHHHHHHHHH
QRLLETGVTLADPARIDVRGELTCGRDVSIDIGCVFEGRVHLGDGVQIGANCVIRNSSID
HHHHHCCCEECCCCEEEEEEEEEECCCCEEEEEEEEECEEEECCCEEECCEEEEECCCCC
AGAQVQPFCHIDSAKIGADGRIGPYARLRPGTELGEDVHIGNFVEVKNSQVAAHSKANHL
CCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEECCCCCCE
AYVGDATVGARVNIGAGTITCNYDGANKFRTVIEDDVFIGSDTQLVAPVTVRRGATIGAG
EEECCCCCCEEEEECCEEEEEECCCCHHHHHHHCCCEEECCCCEEEEEEEEECCCEECCC
TTLTKEAPADKLTLSRAKQMTLDAWQRPVKKAKQ
CEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIVILAAGMGKRMHSDLPKVLHPVAGRPMLAHVIDTARTLSPSRLVVVIGHGAERVREA
CEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHH
VAADDVTFAEQDQQLGTGHAVMQALPQLDDNQPTLVLYGDVPLTTAATLKALVEAAGNTR
HHHCCCEEHHCHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCE
FGVLTVEMPDPTGYGRIVRDAAGSIVRIVEQKDASEAVRAIREINTGIIVCPTGHLRRWL
EEEEEEECCCCCCCCHHHHHHCCCEEEEHHHCCHHHHHHHHHHCCCCEEEECCHHHHHHH
ATLRNDNAQGEYYLTDTVERAATEGIDIVSAQPAALWETLGVNSKVQLAEVERIHQRNLA
HHHHCCCCCCCEEEEHHHHHHHHCCCEEECCCCHHHHHHHCCCCCEEHHHHHHHHHHHHH
QRLLETGVTLADPARIDVRGELTCGRDVSIDIGCVFEGRVHLGDGVQIGANCVIRNSSID
HHHHHCCCEECCCCEEEEEEEEEECCCCEEEEEEEEECEEEECCCEEECCEEEEECCCCC
AGAQVQPFCHIDSAKIGADGRIGPYARLRPGTELGEDVHIGNFVEVKNSQVAAHSKANHL
CCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEECCCCCCE
AYVGDATVGARVNIGAGTITCNYDGANKFRTVIEDDVFIGSDTQLVAPVTVRRGATIGAG
EEECCCCCCEEEEECCEEEEEECCCCHHHHHHHCCCEEECCCCEEEEEEEEECCCEECCC
TTLTKEAPADKLTLSRAKQMTLDAWQRPVKKAKQ
CEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA