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The map label for this gene is thiE [H]

Identifier: 73539915

GI number: 73539915

Start: 236271

End: 237407

Strand: Direct

Name: thiE [H]

Synonym: Reut_A0209

Alternate gene names: 73539915

Gene position: 236271-237407 (Clockwise)

Preceding gene: 73539914

Following gene: 73539916

Centisome position: 6.21

GC content: 67.81

Gene sequence:

>1137_bases
ATGACCCGCCACGCGGTGCACGCCCCGAGCGACGGCCCGCTGCGCCAGGCCGTGCTTGAACACTACGGCGAGACCTTCGG
CGTGTCGGATCATCCGTGGCAGGCCTGGCGGCTTGCCGATGCGCCCGCCAGCCCCGGCGTACACGACGTGCTGCTGTCGG
ATGGTGAGGCCGATGCCGACCTGATCGCACGTGTTGGGGCCGCCGGTGCCACGCTCATCGAGACAGATCGCGAAGGCGGT
CAATGGATCGACACCGTGCGTTCCCCGATGGGCACGTGGGTGCTTTCGTCGCACGCTGATGATGACCACGCGCACTCACC
GGCGTTTGTCGCGGTGCTGCTGGCCTGTCTGTCGCTGCATTTCCCGGCGCATGACGCACTGTGCCTCGCACGTGCGTGGC
GGCCGGGGTCGGCGGAATGGCCGTCCGAGTTCGAGCGCTTCCCGCGCGTGCGCCATGCCGCGCTGGTGGCGCCGGAAGCC
GCTACGCAAGCCTTCGCGCCGTGCCCGCCGGCACTGGGCCTCTACGCCGTGGTGCCGAGCGCCGAATGGATCGAACGCCT
GGTGCCGCTGGGCGTTCCGACGGTGCAGCTGCGCTTCAAGTCGGATGACCCGGCCGCTGTGCGCGCGGAAATCGCGCGTG
CCGCGCAGGCTGCAAAGGGATCGCAGTCCCGGCTGTTCATCAACGACCACTGGCAGGTGGCTGTTGAATTGCACGCCGCC
GCTGGCGGCGACAGCGGCATCTATGGCATCCACCTCGGTCAGGAAGACCTCGACAATGCGGATCTTGATGCGCTGCGCGC
TTCGGGGCTCAGGCTCGGCGTGTCGACGCATGGCTATGCGGAGATGCTGCGCGTGGCGGCGATTCGGCCTAGCTATCTGG
CGTTGGGCGCGATCTTCCCGACGACTACCAAGGTGATGCCTACGGCGCCGCAGGGGATGGGGCGGTTCCGGGCGTATGCG
AAGGTGATGCAGCCAGTGATTCCGTCGTTGGTGGGTATTGGGGGGGTGAATGCTTCCAATATGGGGCAGGTGCTGGCGGT
GGGCGTCCGCAGCGCTGCGGTGGTCCGTGCGATCACTGAAGCCGACGATGTCCCGGCGGCCGTCGCACACCTGATCAGCC
TTTTCCCGGCAGCGTAA

Upstream 100 bases:

>100_bases
CCTATCTGGCCGGCCCGATGCCCGAGCGCGAAGTCGCGCAGGCGAGCACCCCGGTGGTCGGCATGCCGTTCTGGCACGCC
GAAAACACGGAGCACCGCGC

Downstream 100 bases:

>100_bases
GCGCCGGCCCCCTTCGTCGTCCGCCAGGCCACGGCGGACGACGTGCCGCATGTTCTCGCCGCGATCTGGCTGCAACTGCG
CTGTTCCCGGATATCGACCT

Product: thiamine-phosphate diphosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTRHAVHAPSDGPLRQAVLEHYGETFGVSDHPWQAWRLADAPASPGVHDVLLSDGEADADLIARVGAAGATLIETDREGG
QWIDTVRSPMGTWVLSSHADDDHAHSPAFVAVLLACLSLHFPAHDALCLARAWRPGSAEWPSEFERFPRVRHAALVAPEA
ATQAFAPCPPALGLYAVVPSAEWIERLVPLGVPTVQLRFKSDDPAAVRAEIARAAQAAKGSQSRLFINDHWQVAVELHAA
AGGDSGIYGIHLGQEDLDNADLDALRASGLRLGVSTHGYAEMLRVAAIRPSYLALGAIFPTTTKVMPTAPQGMGRFRAYA
KVMQPVIPSLVGIGGVNASNMGQVLAVGVRSAAVVRAITEADDVPAAVAHLISLFPAA

Sequences:

>Translated_378_residues
MTRHAVHAPSDGPLRQAVLEHYGETFGVSDHPWQAWRLADAPASPGVHDVLLSDGEADADLIARVGAAGATLIETDREGG
QWIDTVRSPMGTWVLSSHADDDHAHSPAFVAVLLACLSLHFPAHDALCLARAWRPGSAEWPSEFERFPRVRHAALVAPEA
ATQAFAPCPPALGLYAVVPSAEWIERLVPLGVPTVQLRFKSDDPAAVRAEIARAAQAAKGSQSRLFINDHWQVAVELHAA
AGGDSGIYGIHLGQEDLDNADLDALRASGLRLGVSTHGYAEMLRVAAIRPSYLALGAIFPTTTKVMPTAPQGMGRFRAYA
KVMQPVIPSLVGIGGVNASNMGQVLAVGVRSAAVVRAITEADDVPAAVAHLISLFPAA
>Mature_377_residues
TRHAVHAPSDGPLRQAVLEHYGETFGVSDHPWQAWRLADAPASPGVHDVLLSDGEADADLIARVGAAGATLIETDREGGQ
WIDTVRSPMGTWVLSSHADDDHAHSPAFVAVLLACLSLHFPAHDALCLARAWRPGSAEWPSEFERFPRVRHAALVAPEAA
TQAFAPCPPALGLYAVVPSAEWIERLVPLGVPTVQLRFKSDDPAAVRAEIARAAQAAKGSQSRLFINDHWQVAVELHAAA
GGDSGIYGIHLGQEDLDNADLDALRASGLRLGVSTHGYAEMLRVAAIRPSYLALGAIFPTTTKVMPTAPQGMGRFRAYAK
VMQPVIPSLVGIGGVNASNMGQVLAVGVRSAAVVRAITEADDVPAAVAHLISLFPAA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=210, Percent_Identity=46.1904761904762, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 39926; Mature: 39794

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRHAVHAPSDGPLRQAVLEHYGETFGVSDHPWQAWRLADAPASPGVHDVLLSDGEADAD
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHEEECCCCCCCCHHHHEECCCCCCHH
LIARVGAAGATLIETDREGGQWIDTVRSPMGTWVLSSHADDDHAHSPAFVAVLLACLSLH
HHHHHCCCCCEEEECCCCCCHHHHHHHCCHHCEEEECCCCCCCCCCHHHHHHHHHHHHHC
FPAHDALCLARAWRPGSAEWPSEFERFPRVRHAALVAPEAATQAFAPCPPALGLYAVVPS
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEECCC
AEWIERLVPLGVPTVQLRFKSDDPAAVRAEIARAAQAAKGSQSRLFINDHWQVAVELHAA
HHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEEEEEC
AGGDSGIYGIHLGQEDLDNADLDALRASGLRLGVSTHGYAEMLRVAAIRPSYLALGAIFP
CCCCCCEEEEECCHHHCCCCCHHHHHHCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHCC
TTTKVMPTAPQGMGRFRAYAKVMQPVIPSLVGIGGVNASNMGQVLAVGVRSAAVVRAITE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ADDVPAAVAHLISLFPAA
CCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRHAVHAPSDGPLRQAVLEHYGETFGVSDHPWQAWRLADAPASPGVHDVLLSDGEADAD
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHEEECCCCCCCCHHHHEECCCCCCHH
LIARVGAAGATLIETDREGGQWIDTVRSPMGTWVLSSHADDDHAHSPAFVAVLLACLSLH
HHHHHCCCCCEEEECCCCCCHHHHHHHCCHHCEEEECCCCCCCCCCHHHHHHHHHHHHHC
FPAHDALCLARAWRPGSAEWPSEFERFPRVRHAALVAPEAATQAFAPCPPALGLYAVVPS
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEECCC
AEWIERLVPLGVPTVQLRFKSDDPAAVRAEIARAAQAAKGSQSRLFINDHWQVAVELHAA
HHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEEEEEC
AGGDSGIYGIHLGQEDLDNADLDALRASGLRLGVSTHGYAEMLRVAAIRPSYLALGAIFP
CCCCCCEEEEECCHHHCCCCCHHHHHHCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHCC
TTTKVMPTAPQGMGRFRAYAKVMQPVIPSLVGIGGVNASNMGQVLAVGVRSAAVVRAITE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ADDVPAAVAHLISLFPAA
CCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA